modeling compensation and control of smart devices with hysteresis
SUPERCONDUCTORS – MATERIALS, PROPERTIES AND APPLICATIONS pps
... of each holes due to existence of disorder Figure Schematic of interference between junctions with Josephson couplings J and J' This is a periodic function of Φ [cos2(2πΦ/Φ0)] with a period of ... FeSex with different Se contents, along with the standards Se metal and SeO2 and (d) the first derivative plots of the same spectra Figure 3(c) shows the Se K-edge spectra of the FeSex crystals and ... L2,3-XES of Fe2O3, FeSe1-yTey and Fe metal (b) Ratio of integrated intensities (I2/I3) of XES L2 and L3 lines X-Ray Spectroscopy Studies of Iron Chalcogenides 33 The similarity between XAS and XES of...
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Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
... allosteric control, and that this allosteric control arises from the homotropic effects of one of the substrates, i.e pantoate [8,9] One of the interesting features of the plant PSs is the presence of ... A-chain and B-chain of nPS, and in the case of His37, the v1 torsion angle changes from 63.34° to )67.80° and )52.11° for the A-chain and B-chain of nPS, respectively The imidazole ring of His34 ... shift of the proton, and DdN is the change in the chemical shift of the nitrogen [56] Modeling and comparison of the structures of PSs dali [57] was used to superimpose the crystal structures of...
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Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
... Ala and Glu, Glu and Asp, and Glu and Gln is efficient in wheat germ extract but can effectively be suppressed by inhibitors of transaminases and glutamine synthase [20] Among the multitude of ... observation and dynamics of specific amide protons in T4 lysozyme Biochemistry 24, 817–822 McIntosh LP & Dahlquist FW (1990) Biosynthetic incorporation of 15N and 13C for assignment and interpretation of ... cell-free systems allow the synthesis of [15N]-labelled proteins with very small quantities of [15N]-amino acids and they can be directly measured by NMR without chromatographic isolation or concentration...
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Báo cáo khoa học: Characterization of oligosaccharides from the chondroitin/dermatan sulfates 1 H-NMR and 13 C-NMR studies of reduced trisaccharides and hexasaccharides doc
... region of CS is a repeating disaccharide of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc) [-4)GlcA(b1– 3)GalNAc(b1-]n, which may be O-sulfated on the C4 and ⁄ or C6 of GalNAc and C2 of ... levels of 6-O-sulfation are found ( 70%), with 4-O-sulfation making up the balance along with 25% 2-sulfation of the uronic acid residues [4], and in tracheal cartilage lower levels of 6-Osulfation ... allowing an understanding of species, tissue, age and pathology related differences and the estimation of changes in CS ⁄ DS abundance and composition However, the reduction of the polymer to its...
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Báo cáo khóa học: C-, 15N- and 31P-NMR studies of oxidized and reduced low molecular mass thioredoxin reductase and some mutant proteins docx
... comparison with both TARFH2 and FMNH2, are C(5a), C(7), C(9a) and C(10a), and as already mentioned above, C(2) (Fig 7B) With respect to the 13C chemical shifts of FMNH2, C(2), C(5a), C(9a) and C(10a) of ... higher eld than those of FMN in water and TARF in chloroform (Table 3, Fig 6A) With respect to FMN, the chemical shifts of the N(5) and N(1) atoms are upeld shifted by 7.9 p.p.m and 7.8 p.p.m., respectively ... structures of FAD bound to native and mutant proteins Experimental procedures Purication of thioredoxin reductase and reconstitution with [U-15N4,U-13C17]FAD Fig Representation of TrxR in the FO and...
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Báo cáo khoa học: High-resolution NMR studies of the zinc-binding site of the Alzheimer’s amyloid b-peptide pdf
... HSQC spectra of the aromatic regions of 50 lM Ab(1–40) in 10 mM phosphate buffer at pH 7.4 and 286 K without (black) and with (red) 30 lM zinc as chloride (red) The d and e resonances of the histidines ... aromatic ring of Tyr10 is not involved in binding of the metal Instead, the e and d resonances of the histidines show significant intensity losses, of > 80% The d-proton signals of His6 and His13 ... interaction with zinc [25] Another N-terminal fragment of Ab, Ab(1–16), forms stable oligomers in the presence of both copper and zinc [26] On the other hand, binding of low concentrations of copper...
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Báo cáo khoa học: P NMR studies of energy metabolism in xanthosine5¢-monophosphate overproducing Corynebacterium ammoniagenes pot
... (UDP-glc), b and c phosphate of adenine nucleotide phosphates (ATPb, and ADPb plus ATPc, respectively) The spectrum consisted of 1280 scans and was acquired at 161 MHz with a spectral width of 16 kHz, ... 0.31 molar ratio of the glu/glc was standardized for the in vivo 31P NMR measurements to validate the effect of glu on XMP production Changes in D together with glc, glu, XMP, and hypoxanthine ... substrates, and products of C ammoniagenes in the XMP-production phase (A) Growth (j), residual glc (s), and residual glu (h) (B) XMP (j) and hypoxanthine (m) production (C) Glc (s) and glu (h)...
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Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx
... NP and MP and 2248 between HP and HPO in accordance with the similar shapes and sizes between NP and MP and between HP and HPO The different Kd values are proportional to the different values of ... formation of HP) using both DHNP and DHMP as the substrates and the reverse reaction (the formation of DHNP and DHMP) with HP and GA For the forward reaction, three concentrations each for DHNP and ... and DHMP, respectively, and measured the binding of HP in the absence of GA NP and MP are the oxidized forms of the pterins, with a double bond between C7 and N8 instead of a single bond as in...
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Báo cáo khoa học: NMR and molecular dynamics studies of an autoimmune myelin basic protein peptide and its antagonist Structural implications for the MHC II (I-Au)–peptide complex from docking calculations ppt
... ensemble of structures is the presence of two conformational families with different orientations of the side chain of Glu82 We termed these two families of conformers (#1) and (#2) (Fig 1A, black and ... chain of Arg78 The side chain of Arg78 is less well defined, as in the case of aqueous solution, because of the absence of interactions with the side chains of Asp81 (in the agonist analogue) and ... the backbone of the peptide The origin of this structural orientation in Me2SO is also the strong electrostatic interactions of the side chain of Arg78 with the side chains of Asp81 and Glu82 [this...
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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
... interacts with the region of H1 and the O-methyl group The crystal structure of EW29Ch with lactose showed that the O2, O3 and O4 atoms of the galactose residue of lactose formed hydrogen bonds with ... Interaction of EW29Ch with sugars Discussion Fig 1D STD–NMR spectrum for the mixture of b-Me-Gal (5 mM) and EW29Ch (50 lM) (A) Reference NMR spectrum of the mixture of b-Me-Gal and EW29Ch at a ratio of ... saturation with ms intervals and a total saturation time of s On-resonance irradiation of the protein was conducted at a chemical shift of –0.4 p.p.m and off-resonance at a chemical shift of 30...
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Structural and equilibrium unfolding studies of sam domain of DLC1 by NMR spectroscopy
... 3.10 Plots of the number of assigned NOEs of (a) SAM60 and (b) SAM76 as a function of the range of NOEs and the residue numbers (lower panel), respectively 82 Figure 3.11 Sequential and medium-range ... our lab and the basics of chromatographic and biophysical methods Thanks to Dr Xu Yingqi, Dr Lin Zhi, Dr Zhang Xu and Zheng Yu for helping me with the theoretical and practical basics of protein ... 1.4 Scope of research and outline of the thesis CHAPTER MATERIALS AND METHODS 2.1 Media 2.2 Expression vector construction 2.3 Expression and purification of DLC1-SAM 2.3.1 Expression of DLC1-SAM...
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Báo cáo y học: "Medical resource utilization among patients with ventilator-associated pneumonia: pooled analysis of randomized studies of doripenem versus comparators"
... the study and takes responsibility for the integrity of the data and the accuracy of the data analysis MK contributed to study concept and design, to analysis and interpretation of data and to drafting ... results of this pooled analysis of two phase III studies indicated that initial use of doripenem in patients with VAP was associated with shorter durations of mechanical ventilation and hospitalization ... drafting of the manuscript and critical revision for important intellectual content DN contributed to study concept and design, to analysis and interpretation of data and to drafting of the manuscript...
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Báo cáo y học: "Characterization of N200 and P300: Selected Studies of the Event-Related Potential Salil H. Patel 1 and Pierre N. Azzam 2"
... levels of active latency were associated with concentration ability, and passive latency with verbal proficiency and recall [72] The decreases in P3 and novelty P3 with increasing age, and indeed ... descriptive and limited in power and generalizability Still lacking are large-scale, blinded, randomized, prospective studies of ERP-guided therapy; however with decreasing costs of signal acquisition and ... state of CNS dis-inhibition involved in the pathophysiology of the condition [93] Genetic linkage studies involving families with a history of alcoholism show involvement of chromosomes and and...
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Tài liệu SURVEY OF CASE STUDIES OF THE USE OF KNOWLEDGE MANAGEMENT IN SOFTWARE ENGINEERING docx
... project with a peak manning of 50 people, and with an estimated eort of 7800 mandays The survey was done with a questionnaire, but it is unclear what kind of questionnaire was used, and how many ... ICL and Telenor Telecom Software has descriptions of technical work processes in them, and at sd&m and ICL Finland, we nd lists of employees skills, as well as lists of customers, partners and ... productivity and quality of the developed software The outcome of several of these improvement initiatives was summed up in an article in Communications of the ACM [10] Claims of order of magnitude...
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Tài liệu STUDIES OF AMERICAN FUNGI, MUSHROOMS, EDIBLE, POISONOUS, ETC ppt
... convex, and expanded or nearly flat, and often with an elevation or umbo in the center It is thin, smooth, whitish and often tinged more or less deeply with yellow (sulfur or ochraceous) and is ... the side of the cords These enlarge and elongate and make their way toward the surface of the ground They are at first very minute and grow from the size of a pinhead to that of a pea, and larger ... plants is hairy, of the same texture as the surface of the pileus, torn and mostly clinging to the margin of the pileus, and disappearing with age The general habit and different stages of development...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... Asp142, 2¢ and 3¢OH of the ribose and three water molecules with ˚ coordination distances of approximately 2.4 A The phosphate of the GMP is hydrogen bonded to main chain and side chain atoms of amino ... the base of bound GMP is stacked between Val143 and Phe194 N1, N2 and O6 of the nucleotide form hydrogen bonds to main chain atoms, whereas the side chain of Lys173 interacts with both O6 and N7 ... a b-ribbon with b5, stabilizing loop II The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix...
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Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc
... components of the reductive TCA cycle and catalyze the anabolic reductive carboxylation of acetyl-CoA and succinyl-CoA, respectively [17,18] Four of the five subunits correspond to those of the heterotetrameric ... assay with OGOR and lactate dehydrogenase (LDH; EC 1.1.1.27) Because the synthesis of pyruvate from acetyl-CoA and CO2 is an energetically unfavorable reaction with a reduction potential of )540 ... disappeared and a new signal attributed to the reduced S = ⁄ [4Fe-4S]1+ clusters was observed (Fig 3B) This new signal is an overlap of a major signal with g-values of 1.910, 1.922 and 2.040 and a...
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Tài liệu A REVIEW OF INDOOR AIR POLLUTION AND HEALTH PROBLEMS FROM THE VIEWPOINT OF ENVIRONMENTAL HYGIENE: FOCUSING ON THE STUDIES OF INDOOR AIR ENVIRONMENT IN JAPAN COMPARED TO THOSE OF FOREIGN COUNTRIES pptx
... heating and power generation is considered the main source of SO2 and particulate contamination, which damage human health.52) Domestic sources of SO2 are associated with the use of coal and other ... flammable and reactive compound that is miscible in water and one of the most common solvents, with the chemical formula C2 H4 O.104) It occurs naturally in ripe fruit, coffee, and bread, and is ... 2003, the Ministry of Land, Infrastructure and Transport of Japan amended the Building Standard Law to control indoor chemical concentrations This regulation restricts the use of formaldehydeemitting...
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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... twofold for both substrates Phosphorylation of nucleosides and nucleoside analogues Wild-type Dm-dNK and all mutants, with the exception of E52H, were tested in a phosphotransferase assay with ... Kinetics of dThd and dCyd phosphorylation for active-site mutants of Dm-dNK AraC, AraT and BVDU were tested only with the Y70W mutant kcat values were determined using a calculated mass of 26 785 ... mutation of deoxyribonucleoside kinase L Egeblad-Welin et al Mutants of substrate-binding residues: Y70 and Q81 Y70W showed an increase in Km values for both dThd and dCyd, of approximately 200- and...
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Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx
... without enzyme is in lanes 1, and 5, respectively (B) Substrate incubated with and without 14 nM VsEndA, as explained for VcEndA 10 30 40 50 Temperature (°C) 60 Fig DSC endotherms of VsEndA and ... characterization of the enzymes, we carried out an analysis of the NaCl requirements and pH optimum of VsEndA and VcEndA The optimum NaCl concentrations for activity were found to be 425 mm for VsEndA and ... sequences of VsEndA (Fig 1) The theoretical pI was 9.61 for VsEndA and 8.62 for VcEndA Expression and purification From L Escherichia coli culture, a total of 24 and 50 mg pure recombinant VsEndA and...
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