... Embryonic death and the creation ofhumanembryonicstemcells J Clin Invest 2004, 114:1184-1186 Voullaire L, Slater H, Williamson R, Wilton L: Chromosome analysis of blastomeres fromhuman embryos ... a substantial ethical obstacle for the advancement ofhuman ESC research With the vast therapeutic promise ofhuman ESC seen against the destruction ofhuman embryos required to realize such aspirations, ... Cosponsor the "Stem Cell Research Enhancement Act" [letter to Members ofthe Congress ofthe United States] January 19, 2005 Fact Sheet -Embryonic Stem Cell Research U.S Department of Health & Human...
... and MCs derived fromhuman ES cells (hESECs+MCs) The cell mixture was composed of almost the same number of ECs and MCs We resorted the VE-cadherin+ cellsfrom these expanded cells to obtain ... transplanted vascular cells derived fromhuman ES cells (HES-3) Characterization ofthe transplanted vascular cells derived fromhuman ES cells (HES-3) A, Flow cytometric analysis of VE-cadherin and ... vascular cells on the CBF in the ipsilateral side Effects ofthe transplanted vascular cells on the CBF in the ipsilateral side A-C: LDPI analysis ofthe CBF by LDPI evaluated in mice with the scalp...
... functionality of ESCs 2.2 HumanEmbryonicStemCells (hESCs) Embryonicstemcells (ESCs) are derived fromthe inner cell mass ofthe blastocyst-stage embryos and are characterized by their capacity ... characterized by the breakdown ofthe joint’s cartilage Cartilage is the part ofthe joint that cushions the ends ofthe bones and allows easy movement of joints The breakdown of cartilage causes the bones ... embryonicstemcells (hESCs) are stem cell lines ofembryonic origin, isolated fromthe inner cell mass ofthe blastocyst-stage embryo (Thomson et al., 1998) Human ESCs have the capacity to proliferate...
... of ATP in the dynamics ofthe actin filaments ofthe cytoskeleton Biol Chem 2006;387(4):401-6 Lopez LA, Bertini F The in vivo effect of low body temperatures on the degree of polymerization of ... VS, Jones JM Embryonicstem cell lines derived fromhuman blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonicstem cell lines fromhuman blastocysts: ... Virtually all ofthe detached cells were determined to be non-viable with tryphan blue staining (data not shown) All ofthe detached cells were washed off prior to the MTT assay Fromthe raw absorbance...
... are names ofhumanembryonicstem (hES) cells lines P denoted the number of passages ofthe cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates the time ... development of immune system [15] Signature miRNAs, such as the miR-302 family, the miR-200 family have been reported in human [16,17] and mouse embryonicstemcells [18-20] The unique patterns of miRNA ... names of hES cells lines P denotes the number of passages ofthe cell lines H9-EB denotes embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture HMVEC = human...
... embryonicstem cell; hESC, humanembryonicstem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of ... embryonicstem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of ... epigenomic profiling of a cohort ofhumanembryonicstem cell (hESC) lines can be used to generate hESC reference epigenomes (left panels) The extent of reprogramming and differentiation potential of individual...
... Characterization ofstemcells by derivation source Category EmbryonicstemcellsEmbryonic germ cells Fetal stemcells Umbilical cord stemcells Umbilical cord blood stemcells Umbilical cord matrix stemcells ... to their derivation sources, named embryonicstemcells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stemcells and induced pluripotent stem ... types ofcells which are in the closely related families (Sorgner, 2007) Embryonicstemcells Mesenchymal stem cells, Hematopoietic stemcells Oligopotent Can differentiate into only a few cells...
... 2006) 2.7 Embryonicstemcells and induced pluripotent stemcellsEmbryonicstemcells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable of long-term ... understanding ofthe role of SHH in humans, that is based largely on the mouse model Figure 2.6 Humanembryonicstemcells (hESC) derived fromthe blastocyst are able to differentiate into cellsfrom ... obtain the conditioned media (CM) used for daily hESC culture feeding 3.2.3 Humanembryonicstemcells and induced pluripotent stemcellsHumanembryonicstem cell line HES-3 (46, XX) was from...
... specific diseases in the course of ex vivo gene therapy 1.1.1 HumanEmbryonicStemCellsHumanembryonicstem (hES) cells are cells derived fromthe inner cell mass ofhuman blastocysts The hES cell ... thehumanembryonicstemcells were investigated The transduction ofhumanembryonicstemcells by baculovirus vectors had no effects on the cell growth and pluripotency The formation of teratoma ... in humanembryonicstemcells for up to moths We also characterized the integration sites ofthe transgene expression cassette VII Finally, the effects ofthe baculoviral transduction ofthe human...
... classes of pluripotent stemcells in humans, other primates and mice These are embryonicstem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of ... performed in thehuman Animal embryonicstemcellsEmbryonicstemcells were first derived from certain strains of mice (Evans & Kaufman 1981) Embryonicstem cell lines have been established fromthe ... behaviour to embryonicstemcells served as a model to isolate comparable cellsfrom mammalian embryos The first report on the growth of ICMs and theisolationofstemcellsfromhuman blastocysts...
... events was made possible with theisolationofembryonicstemcells Extensive investigation ofthe properties and capabilities of these cells has led to the development of several experimental approaches ... stagesofembryonic development from fertilization to E6.5 are represented in this image EmbryonicStem (ES) cells are derived fromthe inner cell mass while epiblast stemcells (EpiSCs) are of epiblast ... in the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin ofhumanembryonicstemcells and their in vitro characterisation Figure 1.2 Lineage tree of embryo- derived cells and cell lines Figure...
... understanding ofthe mechanism underlying the lineage specific differentiation of hES cells is mandatory 1.5 HumanEmbryonicstemcells As the name suggests, thehumanembryonicstemcells are derived from ... regenerative therapy include (1) the adult stemcellsfromthe adult tissue (autologous or allogenic) and fromthe bone marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonicstem ... and (2) embryonicstem and embryonic germ cells derived from discarded human embryos and germ line stemcells Adult stemcells can be derived fromvarious parts ofthe body including bone marrow,...
... Introduction 1.1 Humanembryonicstemcells 1.1.1 Overview and characteristics ofhumanembryonicstemcellsEmbryonicstem (ES) cells are isolated fromthe inner cell mass (ICM) of embryos of blastocyst ... HES3 HumanEmbryonicStemCells Undifferentiated HES4 HumanEmbryonicStemCells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_H13_normal_p22_CL_SHE15 ... indicated the uniqueness ofthehuman ES cellsfromthe rest ofthe cells, cancerous or normal and that human EC cells shared a common core set of pluripotency genes with human ES cells In the ES/EC...
... ES cells and show that these marks occur independently ofthe recruitment of any lineage-specific transcription factors such as PU.1 Furthermore, they observe the presence of components ofthe ... recruitment of factors to the a-globin gene locus during the differentiation of erythroid cellsThe regulatory elements located upstream ofthe a-globin genes also appear to initiate the activation ofthe ... reverse-transcribed total RNA extracted from differentiating embryonicstemcells at the indicated time points All primer sets span introns, with the exception of Rex-1, and the size of each RT-PCR product is...
... study ofembryonicstemcells Differences in DNA methylation profiles between ES and other stemcells Comparing ES cells with other stemcells established from developing embryos revealed the uniqueness ... between ES cells and other stemcells derived from developing embryos ES cells derived fromthe inner cell mass (ICM) of blastocysts and EG cells derived fromthe primordial germ cells (PGCs) ... Although there is an apparent ED between ES cells and EG cells, the ED of TS cells to ES cells (77) is greater than that of EG cells to ES cells (49), confirming the similarity of EG cells to ES cells...
... Megakaryocytes from Murine EmbryonicStemCells 11 Development of Lymphoid Lineages EmbryonicStemCells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Differentiation ofEmbryonicStemCells ... TABLE OF CONTENTS Development of Hematopoietic Repopulating CellsfromEmbryonicStemCellsThe In Vitro Differentiation of Mouse EmbryonicStemCells into Neutrophils 10 Development and Analysis of ... Mouse EmbryonicStemCells YIJING CHEN, JAY L VIVIAN, AND TERRY MAGNUSON 406 Section IV Differentiation of Monkey and HumanEmbryonicStemCells 29 Growth and Differentiation Monkey ES Cellsof Cynomolgus...
... examined the expression profile of HLA molecules on the surface ofhuman ES cells, EB cells and blast-like cells We demonstrated extremely low levels of HLA-A2 expression in the undifferentiated H9 human ... blast cellsThe bipotentiality of hemangioblast and blast cells provides the opportunity to use them in future cellular therapies ofhuman disorders Moreover, the blast cells can possibly find their ... Generation of functional hemangioblasts fromhumanembryonicstemcells Nat Methods 2007, 4(6):501-9 Zambidis ET, Peault B, Park TS, Bunz F, Civin CI: Hematopoietic differentiation ofhumanembryonic stem...
... markers on their surface such as CD34 and CD133 allowing for theisolationof relatively pure populations of these cells for in vitro study ofthe cellular processes in thalassemic stemcells While ... While previous attempts have focused on the cell culture system, in this study the phosphoproteome ofthe primary cellsfromthe immediate collection of HSCs from patient blood or bone marrow was ... characterization of phosphorylation is critical to the elucidation of signal transduction pathways, the understanding ofthe mechanism of disease progression, and the development of therapeutic applications...
... markers on their surface such as CD34 and CD133 allowing for theisolationof relatively pure populations of these cells for in vitro study ofthe cellular processes in thalassemic stemcells While ... While previous attempts have focused on the cell culture system, in this study the phosphoproteome ofthe primary cellsfromthe immediate collection of HSCs from patient blood or bone marrow was ... characterization of phosphorylation is critical to the elucidation of signal transduction pathways, the understanding ofthe mechanism of disease progression, and the development of therapeutic applications...