isolation of genes in aquatic animals using reverse transcription polymerase chain reaction and rapid amplification of cdna ends
Techniques in Aquatic Toxicology Volume II - Section 4 ppt
... (Sand Hill Engineering, Geneva, FL) software interface on a Macintosh operating system Dusk–dawn lighting systems are very useful in reducing stress in aquatic holding and testing facilities, and ... significant progress in the ease, utility, and affordability of obtaining, interpreting, and applying behavioral endpoints in a variety of applications from water quality monitoring to use in toxicity ... NH2 scissoring vibrations of cytosine In- plane ring vibrations of cytosine d, 1576 cm−1 Thymine ring vibrations; adenine NH2 bending and C N stretching vibrations e, 1525 cm−1 Cytosine residue;...
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báo cáo khoa học: " High resolution melting analysis for the detection of EMS induced mutations in wheat SbeIIa genes" doc
... ranging between 40 bp and 240 bp spanning a region of 10 kb; moreover each exon is separated by introns of up to kbp in size In order to limit the number of mutations detected in introns and noting ... substrate binding sites and in the protein secondary structure In Figure we show as an example the case of SBEIIa-D (V398I) : while in wild type protein residue 398 was involved in the b3 strand of the ... seven amino acids involved in the binding and catalytic activity in normal SBEIIa protein, respectively Arg465 and Asp467 Analysis of SBEIIa-transcripts in the knock out mutants Expression of the...
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Báo cáo sinh học: "A microsatellite-based analysis for the detection of selection on BTA1 and BTA20 in northern Eurasian cattle (Bos taurus) populations" doc
... genomic interval of significant LD is comparable with the findings of hitchhiking around two anti-malarial resistance genes in humans [58] and microsatellite hitchhiking mapping in the three-spined ... implemented in the software ARLEQUIN [40] using a Markov chain extension to Fisher’s exact test for R × C contingency tables [41] A total of 100 000 alternative tables were explored with the Markov chain ... http://www.gsejournal.org/content/42/1/32 Page of 14 Figure Detailed view of the extent and significance of LD in the cattle populations using the Haploview 4.0 program Numbers in the blocks indicate the percentage of the LD metric...
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Báo cáo khoa học: "Comparison of Diagnostic Methods for the Detection of Parasites in Fish" pdf
... PCR reaction of 50 µl volume (25µl of master mix, Comparison of Diagnostic Methods for the Detection of Parasites in Fish 2µl of each primer, or µl of template and 18 or 20 µl of water) - Polymerase ... between metacercariae in vitreous humour of eyes (one band) and metacercariae in lens of eyes (two bands) No difference between and µl of DNA was observed Figue Testing of NC2-NC5 primer pair ... humours, intestine, fins, skins and the abdominal cavity of fish The detected parasite types include eye flukes, tapeworm, roundworm, crustacean and metacercariae of trematodes However, names of cyst1,...
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Báo cáo khoa học: "Development of immunoassays for the detection of kanamycin in veterinary fields" pps
... ta nim 03 rof detabucni erew setalp eht ,yltneuqesbuS llew hcae ot dedda saw noitulos etartsbus )]dica cinoflus enilozaihtzneb lyhte-3[id-oniza-2,2( STBA lµ 001 ,gnihsaw retfA h rof esadixorep ... etis-A eht ni egnahc lanoitamrofnoc lacol a secudni gnidnib nicymomoraP DJ isilguP ,S awazihsoY ,D ymruoF 054-344 ,36 ,9791 scirtaideP yduts pu-wollof dellortnoc raey-ruof a fo stluser :nicymanak ... dohtem gnineercs elpmis dna dipar a sa ydobitna detagujnoc -dlog ladiolloc gnisu depoleved saw yassa cihpargotamorhc -onummi na ;klim dna amsalp lamina ni nicymanak fo noitceted eht rof yassa...
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Báo cáo y học: "Computerized two-lead resting ECG analysis for the detection of coronary artery stenosis" pps
... containing the abnormal index patterns of tens of thousands of patients with known and clinically verified diagnoses as well as with the patterns of several thousand normal individuals, male and ... Systematic review and modelling of the investigation of acute and chronic chest pain presenting in primary care Health Technol Assess 2004; 8: 1-158 23 Anthony D Diagnosis and screening of coronary ... screening or monitoring of CAD to determine the level of disease or quantifying the patient’s myocardial ischemic burden at the time of the testing Resting ECG analysis, including that of the...
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Báo cáo y học: " Computerized two-lead resting ECG analysis for the detection of coronary artery stenosis after coronary revascularization" doc
... to 2000 in more than 30 institutions in Europe, Asia, and North America on individuals of varying ages and degrees of disease state including normal populations [20,21] All ECG analyses in this ... Systematic review and modelling of the investigation of acute and chronic chest pain presenting in primary care Health Technol Assess 2004; 8: 1-158 Anthony D Diagnosis and screening of coronary artery ... the baseline, occurring two or more times, • a single radical deviation away from the baseline 80° episode of ≥ mm from the baseline A marginal tracing was defined by significant baseline fluctuations...
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Báo cáo khoa học: " Comparisons of Sampling Procedures and Time of Sampling for the Detection of Salmonella in Danish Infected Chicken Flocks Raised in Floor Systems" pptx
... numbers of Salmonella positive flocks in routine samples and in 5sock-samples for the remaining 26 broiler flocks In 16 flocks, where Salmonella was detected in the routine samples (ranging from ... weeks of age (Anon 1999a) and used for the primary identification of infected flocks to be included in the study, and in the follow-up samples, typically taken a few days before slaughtering the animals ... where Sal- Comparing routine sock samples and 5-socksamples in broiler flocks More than one Salmonella serovar was found in Table Comparison of results of sampling of 26 Salmonella infected broiler...
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Báo cáo y học: "Comparison of a Two-Lead, Computerized, Resting ECG Signal Analysis Device, the MultiFunction-CardioGramsm or MCG (a.k.a. 3DMP), to Quantitative Coronary Angiography for the Detection of Relevant Coronary Artery Stenosis (70%)
... http://www.medsci.org Int J Med Sci 2009, analyses included in the database were performed using the same “made in USA” equipment as in the included trials and were analyzed using the same software and hardware ... Systematic review and modelling of the investigation of acute and chronic chest pain presenting in primary care Health Technol Assess 2004;8: 1-158 Anthony D Diagnosis and screening of coronary artery ... is also an increasing cause of concern in the developing world [2] In the USA alone the prevalence of CAD is estimated at 5.9% of all Caucasians of age 18 and older [3] Accurate, non-invasive diagnosis...
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Genotoxicity of 255 chemicals in the Salmonella microsome test (Ames test) and 8-hydroxyguanine (8-OH-Gua) assay for the detection of carcinogens
... Identification of C8-modified deoxyinosine and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and the formation of the adducts in isolated ... Ames test using these sensitive strains (especially YG1041 and/ or YG1042) in combination with the standard tester strains (TA98 and TA100 series), in order to detect the mutagenicity of environmental ... genotoxicity of chemicals 8-OH-Gua is one of the most critical lesions generated from deoxyguanine (dG) in oxidized DNA which was observed after reaction of DNA, protein and lipids both in vitro and in...
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ag doped wo3-based powder sensor for the detection of no gas in air
... responses of the sensor materials were measured in a ¯owing stream of pure air or in 40 ppm NO in air (60:40 mixture from an air cylinder with another cylinder (BOC special gases) containing ca ... hump obtained in XRD) Typically, as seen in the ®gure, a region containing ca Ê 6.93 A inter-planar separation can be visualized It is interesting to note that these defective lines in WO3 are ... in a flowing stream of 40 ppm NO in air at 100 ml/min 8C at 300 8C The powder mixture was filtered, washed, dried and calcined at 600 8C for h Characteristic FTIR bands confirmed the nature of...
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Báo cáo sinh học: " Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" docx
... obtained using DNA from purified MneRV2 in macaque genomic DNA The RRV results were obtained using DNA from spleen of MmuA01111, naturally infected with RRV The MfaRV2 results were obtained using ... assayed in duplicate and the means were determined Standard deviations were calculated using the sum of the errors of the viral and OSM copy number determinations, as described in Materials and Methods ... against the interleukin-6 homolog of RRV found infrequent and low levels of RRV in PBMC of healthy and SIVinfected rhesus macaques Only two healthy macaques had detectable RRV DNA with levels of...
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báo cáo hóa học:" Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" pot
... obtained using DNA from purified MneRV2 in macaque genomic DNA The RRV results were obtained using DNA from spleen of MmuA01111, naturally infected with RRV The MfaRV2 results were obtained using ... assayed in duplicate and the means were determined Standard deviations were calculated using the sum of the errors of the viral and OSM copy number determinations, as described in Materials and Methods ... against the interleukin-6 homolog of RRV found infrequent and low levels of RRV in PBMC of healthy and SIVinfected rhesus macaques Only two healthy macaques had detectable RRV DNA with levels of...
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Báo cáo hóa học: " Unscented Kalman filter with parameter identifiability analysis for the estimation of multiple parameters in kinetic models" pptx
... similarly determined using a genetic algorithm (GA) and NLSQ Both alternatives were implemented in MATLAB, using the default implementations and settings A third alternative, simulated annealing, was ... to the matrix X L (L being the iteration number), in the order of the highest to the lowest sensitivity To make the adjustment of the net influence of each of the remaining parameters on the already ... recursion the availability of new information during the parameter estimation in UKF correlates to a general decrease in the uncertainty within the system [21], making the standard deviation a feasible...
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Báo cáo khoa học: "Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections" pdf
... in the intestinal tissues from naturally infected piglets Vet Pathol 2000, 37, 62-67 Kim O, Chae C Comparison of reverse transcription polymerase chain reaction, immunohistochemistry, and in situ ... study of pathogenesis and diagnosis of CHV infection References Appel MJG Virus Infections of Carnivores pp 515, Elsevier, Amsterdam, 1987 Kim O Development of in situ nest PCR and comparison of ... enzyme-concentrations, the time of hybridization and hybridization probes, were compared in formalin fixed and paraffin embedded CHV-infected cells The optimum result was obtained using digoxigenin-labeled probe,...
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Báo cáo khoa học: "Development and evaluation of indirect ELISA for the detection of antibodies against Japanese encephalitis virus in swine" pptx
... saw noitcaer cinegomorhc eht dna ,nim 51 rof detabucni erew setalp ehT dedda saw )ASU ,LPK ;tanoflusnilozihtneb-lyhte-3-id-oniza-'2.2( STBA etartsbus eht fo emulov lacitnedi na ,h rof Co73 ta noitabucni ... 1.0/05DICT 002 gniniatnoc noisnepsus suriv 9981VK a fo emulov lauqe na htiw dexim erew ares eht fo snoitulid dlof-owt laireS sllec 401FT gnisu setalporcim llew-69 a ni demrofrep saw nim 03 rof Co65 ta ... sisongaid -ores eht ni esu laitnetop sti rof detagitsevni saw ASILE-I VEJ eht rof depoleved saw dohtem lacigolores tneinevnoc dna elpmis a ,nosaer siht roF selpmas fo rebmun detimil a htiw noitazidradnats...
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Báo cáo khoa học: " A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples" potx
... with 200 μl of wash buffer (PBS containing 0.05% [v/v] Tween-20 and 0.01% bovine serum albumin), and once with 200 μl of PBS To each well, 100 μl of biotinylated capture probe (0.1 μM in 50 mM potassium ... requirements of JD These results indicated that this assay is highly sensitive and could be used to detect animals in the early stage of infection with very low MAP shedding In addition to the rapid ... USA) were cut into 4.5 mm × 7.5 cm strips Streptavidin was diluted in 0.4 M NaHCO3/Na2CO3 buffer (pH 9.0) containing 5% methanol in a final concentration of 20 pmol/μl Streptavidin was spotted...
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Báo cáo y học: "Reliability of computerized image analysis for the evaluation of serial synovial biopsies in randomized controlled trials in rheumatoid arthritis" pps
... advantage of DIA is standardization of image acquisition and processing, minimizing variance, and the ability to quantify the actual stained area together with staining intensity in a time efficient ... Although the reproducibility of measuring cytokine and cell adhesion molecule staining by DIA was reported to be within 10% [8], no formal studies investigating intrarater and inter-rater variability ... t0, OB2 and OB3) Table Estimates of the variance components (between and within patients) and of the intraclass correlations (single rater and average of raters) Cell type Intra-observer Inter-observer...
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Báo cáo khoa học: "Study protocol of the German "Registry for the Detection of Late Sequelae after Radiotherapy in Childhood and " ppsx
... not sufficient in view of answering open questions regarding the risks of radiotherapy in childhood and adolescence Methods/Design To overcome the described lack of information and to receive ... coordinator of RiSK, participated in the development of the study trial and drafted the manuscript AS, HP, CR, BP, BT, RDK and KD participated in the design of the registry study and are involved ... study and are involved in continuing optimization NW is the study chairman of RiSK, developed the design of the registry study, is involved in continuing optimization and helped to draft the...
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Báo cáo sinh học: "Optimal design for the detection of a major gene segregation in crosses" potx
... where both F2 and backcrosses were present The proportiont of individuals belonging to the ’segregating groups’ increased between C10 and C19, C20 and C26, and C3 and C5 The proportion of F2s to ... linear for power under 70% and, in this linear part, the slope (ie the gain in power = = = per extra individual measured) increases with a The resulting increase in size of the design required for ... gene for rapid postweaning growth in mice Genet Res Camb 44, 293-308 Dickerson GE (1973) Inbreeding and heterosis in animals In: Proc Anim Breeding Genet P Sym in honour of Dr JL Lush, Am Soc...
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