... mm) TV MUC (0.5 mm) Submersible box and tube corers (0.3 mm) Submersible box and tube corers (0.3 mm) Submersible box and tube corers (0.3 mm) Submersible box and tube corers (0.3 mm) Methods (mesh ... high and wide A collection of < /b> seven of < /b> these bushes yielded 66 species of < /b> which 18 are considered to be endemic (Bergquist et al 2003) and five (four bivalves and a sponge) appear to harbor symbionts ... understood to be microbially mediated (Barbieri & Cavalazzi 2004) Mats of < /b> filamentous bacteria and bioherms (reefs or aggregations of < /b> clams, tubeworms or mussels) provide biological evidence of < /b> seepage...
... and can be irreversibly adsorbed onto cellulosic substrates Water < /b> soluble cellulose derivatives, vegetable gums, and hemicelluloses, are adsorbed onto fibres in the absence of < /b> electrostatic interactions < /b> ... wood fibres and both the thickness and the microfibril angle of < /b> the S2 layer affect the mechanical strength of < /b> fibres (Burgert et al 2002; Page et al 1977) In delignified and bleached softwood ... these fibril-polymer layers form fibre bonding domains and upon drying, the interactions < /b> between the cellulose fibrils and the polymers will affect the development of < /b> the fibre-fibre bonds Hence,...
... obtained with < /b> both electrochemical methods such as cyclic voltammetry, which probe the electron flow between the immobilized cytochrome c and the electrode, and SERR spectroscopy with < /b> Soret band ... intrinsic stability of < /b> the Fe–S(Met) bond is not significantly affected by biologically meaningful electric fields [51], ˚ homogeneous fields of < /b> 25 mVÆA)1 are able to perturb flexible segments of < /b> the ... M, Heimburg T & Hildebrandt P (2004) Peripheral and integral binding of < /b> cytochrome c to phospholipid vesicles J Phys Chem B 108, 3871–3878 50 Basova LV, Kurnikov IV, Wang L, Ritov VB, Belikova...
... cells for binding of < /b> the extract to the DR1 probe As shown in Fig 3C, a specific complex was observed binding to the probe, which could be competed by unlabelled probe (self) DNA, but not by an unrelated ... binding by either purified recombinant dUSP, or by components of < /b> Sf9 nuclear extracts Binding conditions for recombinant dUSP were 1.0 lg of < /b> dUSP, lg dIdC, lL of < /b> 5· binding buffer (1·: 10 mm of < /b> ... Bombyx EcR (BmEcR) and Bombyx USP (BmCF1) combine to form a functional ecdysone receptor Insect Biochem Mol Biol 26, 217–221 Baker KD, Shewchuk LM, Kozlova T, Makishima M, Hassell A, Wisely B, ...
... redistribute However, for a semiconductor, a narrow band gap exists between the valence band and conduction band In the case of < /b> insulators, a wide energy band gap forbids the relocation of < /b> electrons ... and a metal The lowest unoccupied band is called the conduction band and the highest occupied one the valence band The energy spacing between the highest occupied and the lowest unoccupied band ... spectrum of < /b> the VBV-LDPE film after reaction with < /b> 500 ppm platinum chloride solution under UV < /b> irradiation for 15 Figure 5.8 UV-< /b> visible absorption spectra of < /b> the BV-PVA film before and after UV < /b> irradiation...
... !nn- - No symptoms typical of < /b> forest decline observed were Conclusions Both tures a better water < /b> stress tolerBoth features probably are reasons for the high productivity of < /b> Douglas fir in central ... analyses on shoots of < /b> Picea abies and leaves of < /b> Coflea liberica at various tem- peratures Physiol Plant.70, 189-195 Gross K & Pham-Nguyen T (198 7b) The influence of < /b> constant long-term water < /b> stress ... reduced (Figs 1, 2) The degree of < /b> reduction depended pri- upon intensity of < /b> water < /b> stress but also modified by water < /b> vapor saturation deficit (WSD) and the level of < /b> the turgor loss point (Figs...
... employees and stockholders of < /b> Eli Lilly and Co Ms Garnett is a contractor for Eli Lilly and Co Authors' contributions RB, FB, KR, and JJ participated in the conception and design of < /b> the registry RB, ... supported by Eli Lilly and Company and executed with < /b> the oversight of < /b> a steering committee with < /b> clear governance rules covering data access, ownership, and publication This publication was approved by ... characteristics, with < /b> the exception of < /b> patients with < /b> Gram-negative infections and the primary site of < /b> infection, baseline imbalances were present between patients who received DrotAA and those that...
... significant difference of < /b> the overall codon usage pattern of < /b> HBV between genotypes A, B, E and C, D, G HBV genotypes and subgenotypes have been associated with < /b> differences in clinical and virological ... pattern and evolutional dynamics of < /b> HBV as well as the relationship between codon usage pattern of < /b> HBV and its host Result Synonymous coodn usage in HBV The C% and U% were higher than A% and G%, and ... pattern of < /b> HBV is a mixture of < /b> coincidence and antagonism to that of < /b> host cell But the difference of < /b> genetic characteristic of < /b> HBV failed to be observed to its different epidemic areas or subtypes,...
... chlorinated PCB congeners (Ahlert and Peters, 2001) The solubility of < /b> Aroclor 1260 in water < /b> is reported to be about ppb Effect of < /b> initial H2O2 concentration To evaluate the effects of < /b> initial H2O2 ... PCB degradation may still be obtained even without adjusting the pH of < /b> the aqueous PCB solutions Effect of < /b> Initial PCB Concentration The results of < /b> the degradation of < /b> PCBs at different initial ... process Based from the results of < /b> this phase of < /b> the experiment, the optimum H2O2 is 24.71 mM or 2.8 ml per liter of < /b> the aqueous PCB solution containing about 40 ppm of < /b> pure PCBs Percentage PCB degradation...
... completely understand the fundamental principles of < /b> the photochemical process of < /b> VUV-induced water < /b> treatment In this work, the treatment of < /b> dye wastewaters by the VUV-only and VUV/H2O2 process ... requirements of < /b> the VUV photolysis of < /b> water < /b> according to Eq (1) are related to the formation of < /b> high local concentration of < /b> hydroxyl radicals (OH.) and a series of < /b> other species (Gonzalez and Braun, ... (Snell and Ettre, 1987) The UV < /b> light absorbance of < /b> reacting solutions were detected by a HITACH U-2000 UV/< /b> Visible spectrophotometer RESULTS AND DISCUSSION The experimental works were conducted and...
... disruption of < /b> a key interaction and the perturbation of < /b> flexibility of < /b> CA may be the most effective against HIV-1 The molecular details of < /b> LysRS, CyPA and TRIM5a interactions < /b> with < /b> CA remain incomplete and ... shaped like a b- barrel formed by eight antiparallel beta strands with < /b> two alpha helices that cap the top and bottom of < /b> the barrel (Fig 3) [36,37] The active site, in a hydrophobic pocket on the ... perturbations of < /b> residues in and around helix (211LEEMMT216) of < /b> CA-CTD were observed upon LysRS binding Residues T210, M214 and M215, along with < /b> a nearby H226, were implicated as critical by peptide...
... inhibition of < /b> MAO with < /b> a mechanismbased irreversible inhibitor did not prevent binding of < /b> the I2 ligand 2-(2-benzofuranyl)-2-imidazoline (2-BFI), suggesting that the I2BS was not the active site of < /b> MAO ... Table It has been proposed that I2BS ligands bind both to the MAO active site, thereby inhibiting the enzyme, and to a second site with < /b> substantially higher affinity present on a subpopulation of < /b> ... consistent with < /b> the IC50 values in the literature for membrane-bound enzyme (Table 1) The type of < /b> inhibition and the inhibitory constants (Ki) were determined by varying both substrate and inhibitor...
... photograph of < /b> the RT-PCR products run on B Fig (A) Bar diagrams showing mean of < /b> IOD of < /b> bands obtained by RT-PCR at caspase-8 and caspase-10 loci, along with < /b> error bars calculated on the basis of < /b> three ... 1/[DNA]µM III Fig In vitro binding assay of < /b> putative HIPPI-binding motif AAAGACATG and its mutant (A) EMSA of < /b> binding of < /b> the end-labeled bp motif AAAGACATG and two of < /b> its mutants with < /b> purified GST–HIPPI ... labeled with < /b> [32P]dCTP[aP] was used as probe Lanes and show results obtained with < /b> probe only and probe + 4.8 lM BSA (nonspecific protein), respectively Lane shows the result when 500 nM probe...
... dimerization of < /b> decorin and binding of < /b> both collagens to the inner surface of < /b> decorin (B) Interaction of < /b> type I collagen with < /b> the inner surface of < /b> the decorin core protein and type VI collagen binding with < /b> ... sheets with < /b> an air brush and rotary shadowed with < /b> platinium-carbon at an angle of < /b> about 7°, followed by pure carbon as described by Cohen et al [21] The replicas were placed on uncoated grids and ... index and nonspecific binding, the responses obtained with < /b> immobilized albumin were subtracted from Ó FEBS 2004 Decorin interacting with < /b> type I and VI collagen (Eur J Biochem 271) 3391 those obtained...
... with < /b> the issue First, the role of < /b> industrialisation in polluting freshwater of < /b> China and Brazil will be studied and compared In order to assess how China and Brazil deal with < /b> the problem of < /b> water < /b> ... is the backbone of < /b> the Chinese informal rules Guanxi is “based on a blend of < /b> exchanges and mutual affection that create feelings of < /b> responsibility and obligation on the one hand and indebtedness ... and Brazil to become one of < /b> the biggest contributors to global pollution Every year Chinese rivers, lakes and coastal waters receive about 36 billion tons of < /b> untreated industrial wastewater and...
... associated with < /b> 70S ribosomes and 30S and 50S subunits Table shows the amount of < /b> EF-P that binds to each subunit and to 70S ribosomes The labeled protein binds in an : molar ratio to 70S ribosomes and ... FEBS 673 Ribosome interactions < /b> with < /b> elongation factor EF-P H Aoki et al Table Stoichiometric binding of < /b> EF-P to 70S ribosomes and to 30S and 50S subunits EF-P protein was labeled as described ... followed by 1D SDS electrophoresis and western blotting As shown in Fig 1B, EF-P is bound to the 30S and 50S subunits, to 70S ribosomes and to polyribosomes The density of < /b> the immunoblots was...
... were supplied by Amersham Pharmacia Biotech AB Rabbit antisera pAb against the intracellular domain of < /b> human nephrin has been described previously [1,58] Affinity-purified polyclonal rabbit anti-podocin ... were mixed with < /b> 500–1000 lL of < /b> cell extracts, and incubated for h with < /b> end-to-end mixing at °C After washing with < /b> the lysis buffer, bound proteins were eluted by boiling in the SDS-sample buffer ... which the cells were permeabilized by incubating with < /b> 0.1% (v ⁄ v) Triton X-100 in NaCl ⁄ Pi for Non-specific binding was blocked by incubating cells with < /b> 2% BSA ⁄ 2% fetal bovine serum in NaCl ⁄...
... EGF, bound with < /b> high affinity to both ErbB-1 and ErbB-4 [24] When bound to the receptors, this artificial molecule uses the N-terminal andb sheet parts to bind with < /b> domains I of < /b> ErbB-4 and ErbB-1, ... between EGF and NRG1 -b1 Domain I of < /b> ErbB-1 binds the central b sheet of < /b> EGF, while that of < /b> ErbB-4 recognizes several N-terminal residues of < /b> NRG 1b1 Although the b sheet of < /b> the ligands is located adjacent ... EGF binding On the other hand, chimeras 4111-1 and 4411-1, generated by the replacement of < /b> domain I andof < /b> domains I and II, respectively, of < /b> ErbB-1 by the corresponding domain(s) of < /b> ErbB-4,...
... degree of < /b> b- 17 association with < /b> the membrane systems tested in leakage, a series of < /b> LUVs were made as previously described and aliquots of < /b> a suspension of < /b> LUVs were added sequentially to NBDlabeled ... cuvette, before addition of < /b> LUVs After every addition of < /b> vesicles, the system was allowed to incubate for a few minutes Controls with < /b> addition of < /b> LUVs to buffer only were also made and subtracted ... peptide with < /b> antimicrobial activity, as microbial membranes have a net negative surface charge Membrane binding Fig Extent of < /b> leakage from 25 lM LUVs induced by b- 17 after 300 s as a function of < /b> P/L...
... that HBX is both a substrate and a potential inhibitor of < /b> the proteasome complex The authors suggested that HBX may inhibit proteasomemediated proteolysis by binding two subunits (Sub4, Sub7) of < /b> ... subunit p42 with < /b> SARS-CoV NP in vitro Figure Expression and Identification of < /b> SNP2 2b (SARS-CoV NP) (A) Supernatants (lanes and 10) and pellets (lanes and 6) of < /b> E coli BL21 containing the pET2 2b- SNP2 2b ... polyclonal antibody (Biomol, USA) at 37°C for 60 min, coverslips were washed six times with < /b> 1× PBS After incubation with < /b> a 1:80 dilution of < /b> goat anti-rabbit IgG-FITC (Sigma) and a 1:200 dilution of < /b> goat...