in vitro expansion of alloantigen specific regulatory t cells and their use in prevention of allograft rejection
báo cáo hóa học:" Generation in vivo of peptide-specific cytotoxic T cells and presence of regulatory T cells during vaccination with hTERT (class I and II) peptide-pulsed DCs" pot
... potency in stimulating T cell responses, stability of the phenotype following in vivo administration, the ability to migrate to sites of T cell activation and generation of CTLs Activated and mature ... patients, rather than patient groups Maintaining the levels of TAAg -specific CD8+ T cells in the circulation is the summation of the capacity to generate them and their possible loss (apoptosis ... concluded that hTERT p540 is not expressed or is cryptic on the surface of tumour cells and that immunization of cancer patients with hTERT p540 leads to the production of T cells that not recognize tumour...
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Báo cáo y học: "A paragon of self-tolerance: CD25+CD4+ regulatory T cells and the control of immune responses" pps
... B, Mason D: Regulatory T cells in the control of autoimmunity: the essential role of transforming growth factor beta and interleukin in the prevention of autoimmune thyroiditis in rats by peripheral ... convincingly, retroviral transduction of Foxp3 into conventional CD25– Foxp3– T cells converted them into phenotypical and functional TR cells capable of effectively suppressing both in vitro and ... CD25+CD4+ cells after stimulation through the TCR and might therefore mediate its effects in a membraneproximal manner [53] The level at which these anti-inflammatory cytokines operate to maintain tolerance...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... factors in the supernatant inhibit the maturation of fusion cells and have a negative impact in the stimulation of T cells To determine the induction of WT1-specitic CD8+ T cells, a pentameric ... which Treg arise in vivo and exert their immunoregulatory effects remain to be defined and are the subject of intensive investigation In the present study, we first show that coculture of T cells ... supernatants in the generation of Treg in vitro is demonstrated in the present study, little is known about the impact of fusion cell vaccination on generating Treg The negative impact of fusion...
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Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
... This band spacing appeared to reflect the triplet repeat unit, implying that the in vitro DNA strand slippage syntheses of (AAT)3 ⁄ (ATT)5 and (ATT)3 ⁄ (AAT)5 tracts mainly occurred by triplet step ... greater than that of DDI-1B; (e) the enhancement effects of DDI-1A and DDI-1B on AAT, CAG and CA repeats are stronger than that of the ATT, CTG and GT strand, which may be attributed to the T T ... system, the expanded primary bands were almost all seen in the three-nucleotide unit, which indicated that the in vitro DNA strand slippage synthesis of (ATT)3 ⁄ (AAT)5 tract was mainly a triplet expansion...
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Báo cáo y học: "Nature of Regulatory T Cells in the Context of Allergic Disease." ppsx
... amounts of IL-4 and IL-10.35 Further studies are required to elucidate the in vivo role of these cells and their subsets Effects of SIT on Dendritic Cells To understand the mechanisms of action of ... applicability that will have persistent clinical effectiveness that can be built within a short duration of therapy time.1 107 Early and Late Effects of SIT on Mast Cells, Basophils, and Eosinophils SIT ... from the oral mucosa were not able to transfer tolerance, but they acted as antigen-presenting cells in senso stricto irrespective of the source and route of SIT Induces Specific T- Cell Tolerance...
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Báo cáo y học: "CD25brightCD4+ regulatory T cells are enriched in inflamed joints of patients with chronic rheumatic disease" potx
... enrichment of regulatory T cells was seen Thus, the simple extrapolation of the animal data into future therapeutic strategies that aim at reconstituting this population does not seem to hold true in ... influence the inflammatory processes in the joint These features were apparent in the vast majority of the patients despite the different treatments they received, indicating that the anti-rheumatic ... enrichment of CD25brightCD4+ T cells in the joint of patients with rheumatic disorders suggest that the immune system is actively attempting to control the inflammatory responses by recruiting regulatory...
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Báo cáo y học: "Regulating the immune system: the induction of regulatory T cells in the periphery Jane H Buckner1 and Steven F Ziegler2" pot
... death, TR cells lead to the induction of T regulator (Tr1) cells and Th3 cells, which feed back to inhibit inflammation, and the TR cells inhibit proliferation of antigen -specific and bystander ... differentiating into TR cells following activation are derived from a separate lineage of CD4+ T cells, and whether their induction is a result of the initial activation early in the inflammatory ... when the body is threatened by infection or injury In this setting T cells will become activated, and will recruit other T cells and other inflammatory cells and mediators to the site As the response...
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Báo cáo y học: "The role of regulatory T cells in antigen-induced arthritis: aggravation of arthritis after depletion and amelioration after transfer of CD4+CD25+ T cells" doc
... CD25-expressing cells or transfer of CD4+CD25+ cells, in the present study we demonstrated that Treg cells modulate the onset of AIA but are ineffective at later stages, calling into question their ... necrosis factor family-related gene (GITR) and GITR-ligand interactions, abrogating the suppressive effect of Treg cells [37] With this in mind, it could be interesting to investigate whether the accumulated ... 7b) The migration behaviour of CD4+CD25+ Treg cells does reflect their more activated phenotype, and their ability to enter inflamed joints makes it possible that they act directly at the site of...
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Báo cáo y học: "Role of regulatory T cells in experimental arthritis and implications for clinical use" potx
... might not be a feasible option treatment decreases the infiltrate in joints Furthermore, RA patients relapse shortly after withdrawal of anti-TNF-α [19] and thus, despite the dampening of joint inflammation ... that Treg isolated from patients with active RA before treatment with anti-TNF-α were unable to suppress proinflammatory cytokine secretion from activated T cells and monocytes After anti-TNF-α treatment ... CD4+CD25+ Treg had a suppressive function and accumulated in the inflamed joint [14–17] One of these studies even reported that CD4+CD25+ Treg isolated from joints of patients with active arthritis...
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Báo cáo y học: "Indoleamine 2,3-dioxygenase-expressing dendritic cells are involved in the generation of CD4+CD25+ regulatory T cells in Peyer''''s patches in an orally tolerized, collagen-induced arthritis mouse model" ppt
... determining immunity or immune tolerance; this determination is based on the maturation or activation state and the subset of DCs, and cytokine profiles in the microenvironment at the time of antigen ... expression and inhibit T- cell proliferation In this regard, it will be interesting to investigate IDO expression in different subsets of DCs from tolerized mice and to characterize their role in the induction ... PCR using IDO sense (5'-CACTGTACCAGTGCAGTAG-3') and antisense (5'-ACCATTCACACACT CGTTAT-3') primers, and using Foxp3 sense (5'-CAGCTGCCTACAGTGCCCCTAG-3') and antisense (5'CATTTGCCACGAGTGGGTAG-3')...
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Báo cáo y học: "Japanese Encephalitis Virus wild strain infection suppresses dendritic cells maturation and function, and causes the expansion of regulatory T cells" doc
... IFN-a and TNF-a was independent on viral replication DCs infected with P3 attenuated allostimulatory activities to T cells To test whether P3 infection will impair the ability of DCs to activate ... 0.05 Treg in vivo The results also demonstrated the expansion of Treg population after the co-culture of P3infected DCs and T cells It suggested that JEV infection of DCs might influence the mode ... attempted to address the possible pathogenesis of JEV wild strain infection by testing the interaction of JEV and DCs in vivo and in vitro Carrasco et al., [2004] discovered that CSFV could infect...
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Báo cáo y học: "Analysis of the 5''''UTR of HCV genotype 3 grown in vitro in human B cells, T cells, and macrophages" potx
... different strains or types of HCV We are reporting the isolation and replication of HCV from patients infected by type strains of HCV These new isolates can be cultured in both B and T cells By contrast ... circulating in their blood It is possible that these other genotypes may also prefer to infect specific cell types for replication in vitro Others have shown that different tissues in one particular ... 314 T1 and 314 T2 were the same, showing that consecutive transfers of HCV into the same cell type not affect the sequence The 314 T1 and T2 sequences were almost identical to genotype H77, therefore...
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Báo cáo y học: " CD39+ Regulatory T cells suppress generation and differentiation of Th17 cells in human malignant pleural effusion via a LAP-dependent mechanism" pps
... T cells after culturing in presence both IL-1b and IL-6 (C) The representative dot plots of CD39+Tregs and Th17 cells detected in naïve CD4+ T cells after culturing in presence of TGF-b1 (D) The ... consequence of activation status [34] Furthermore, these markers cannot be used to identify Treg poststimulation in vitro, since their expression patterns change toward the Treg phenotype upon activation ... correlated in MPE, and that the ratios of CD39+Tregs/Th17 cells were significantly lower in MPE than in blood, suggesting that there could be a dynamic interaction between Th17 cells and CD39 + Tregs...
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Báo cáo y học: "haracterization of regulatory T cells in urban newborns" pptx
... supervised patient recruitment for the study and obtained funding JEG participated in study design, data analysis, and obtained funding All of the authors participated in drafting the manuscript and approved ... using SAS, version (SAS Institute, Cary, NC) and the R system for statistical computing [27] Results Subject characteristics The subjects in this study consisted of a subset of newborns and mothers ... recently ignited interest in defining the role of Treg cells in allergy and asthma in humans Most studies on the association between Treg and asthma/allergy have focused on adults [5-8] with allergy...
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Báo cáo sinh học: " Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice" docx
... and mature DCs, resulting in tolerogenic interaction with T cells in SIT We found that the combination of DNA vaccination with Fc and OVA had better effects onOVAinduced alterations in the spleen ... pathway of regulation of type immunity in asthma Competing interests The authors declare that they have no competing interests Authors' contributions YL performed all analyses and wrote the initial ... directly to DCs may a new alternative of therapies for patients with allergic asthma These findings suggest that spleen DCs and Foxp3+Tregs prevents the generation and activation of Th2 effector cells...
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Báo cáo sinh học: " Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneratio" potx
... production through two mechanisms: 1) promoting the direct interaction between monocytes and T cells; 2) indirectly stimulating the production of IL-1b and IL-6 from monocytes C5a can bind to the trans-membrane ... patients C5a may be one of the many factors related to this observed effect Other unknown factors may also contribute to this T cell activation seen in AMD patients Interestingly, the findings ... systemic activation may ultimately be manifest in the eye remain to be defined To date there is no effective treatments other than attempts to slow the progression of geographic atrophy form of...
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Báo cáo y học: "Decreased effector memory CD45RA+CD62L– CD8+ T cells and increased central memory CD45RA–CD62L+ CD8+ T cells in peripheral blood of rheumatoid arthritis patients" ppt
... effector memory CD8+ T cells in RA may indicate an increase in the migration of these cells into sites of inflammation, and therefore may contribute to ongoing synovial inflammation Competing interests ... survival of these cells, or blockade in their differentiation Perturbations in the homeostasis of memory T cells may play an important role in the pathogenesis of RA by generating effector cells that ... http://arthritis-research.com/content/5/2/R91 individuals and in SLE patients, indicating that this perturbation in homeostasis of T cells is a specific feature of RA Although the naïve/memory phenotype of...
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Báo cáo y học: "The potential of human regulatory T cells generated ex vivo as a treatment for lupus and other chronic inflammatory diseases" pot
... activity that was neutralized by anti-TGF-β Activating both CD4+ and CD8+ cells in the presence of TGF-β thus induced them to develop cytokine-dependent The role of transforming growth factor-β ... favor the hypothesis that TGF-β promotes the death of mature Th1 and Th2 cells while protecting newly generated regulatory T cells from undergoing apoptosis This view is consistent with a report indicating ... CD4+ cells are CD25–, why the predominant TGF-β effect on T cells in this fraction is the generation of ‘professional’ regulatory T cells remains to be determined Our finding that both IL-2 and TGF-β...
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Báo cáo y học: "Regulatory T cells in systemic lupus erythematosus: past, present and future" pot
... Overproduction of type I interferons in SLE matures dendritic cells (DCs) into immunogenic antigen-presenting cells that strongly contribute to the T- cell and B-cell hyperactivity characteristic of this ... and the suppressive function of these cells was intact in cultures without antigen-presenting cells The functional activity of both patient and healthy control Tregs, however, was decreased in the ... not be able to overcome the strength of T- cell stimulation or the inhibitory effects of proinflammatory cytokines produced by other cells It is known that strong Toll-like receptor stimulation...
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