gene targeting in human pluripotent stem cells
Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders
... protein The naturally occurring fingers with known binding preference were disassembled to find fingers for all 64 different target triplets to enable the generation of zinc finger domains binding ... lt-NES cells 85 Gene corrected human neurons 86 Zinc finger nucleases for gene targeting in lt-NES cells 89 Zinc finger nucleases (ZFNs) in comparison to Transcription ... group developed a system of adenosine-releasing fibroblasts with inactivated adenosine kinase and adenosine deaminase These cells were transplanted into brain ventricles in a rat epilepsy model...
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Atlas of Human Pluripotent Stem Cells doc
... iPSCs ISCN IVF Inner cell mass Imprinting control region mice Immunohistochemistry Immunofluorescence Inner root sheath Induced pluripotent stem cells International system for human cytogenetic nomenclature ... differentiated human pluripotent stem cells is demonstrated in the subsequent chapter Karyotype stability of human pluripotent stem cells is sensitive to growth conditions and to the manner in which cells ... antibodies into the blastocyst, thus leaving the ICM cells intact Incubation with guinea pig complement-containing medium then lyses all antibodymarked cells The intact ICM is further rinsed and...
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Báo cáo y học: " Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses" doc
... interrupted by the viral components Cytokines and chemokines generally function in an autocrine (on the producing cell itself) or paracrine (on nearby cells) manner Cytokines released following ... for growing stocks of influenza virus isolates MDCK cells were grown and maintained in Eagles Minimal Essential Media (MEM) containing 2% FBS, 100 U/ml penicillin and 100 μg/ml streptomycin (all ... Cytokine/chemokine protein profiles following infection To verify whether changes at the mRNA level were translated to protein level, the protein concentrations of cytokines/chemokines in cell...
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Báo cáo y học: "Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells" pot
... reverse (decoy) IPI human protein database containing the sequences of the proteins harboring the porcine-specific proteins identified in this study Using the same filtering criteria used for ... differences in methods of CM generation A preliminary proteomics study demonstrated that NCA media conditions contained porcine alpha-2macroglobulin, clusterin and tenascin Intriguingly, these proteins ... species, including humans, during growth and aging, the NCs populating the NP disappear and are replaced by chondrocytic NP cells [33] The NP of some species retain notochord cells into maturity,...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) ... al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable ... forebrain, midbrain, hindbrain and the spinal cord This figure was reproduced from Epstein et al., 1999 2.4 SHH and neural development During the initial phase of neural induction, the ectoderm is induced...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain stem cells ... experiments involving blastocyst injections and ectopic grafting in adult hosts cannot be performed in the human Animal embryonic stem cells Embryonic stem cells were first derived from certain strains ... into a totipotent state Definition of a stem cell Three basic categories of cells make up the human body viz., germ cells, somatic cells and stem cells Somatic cells include the bulk of the cells...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly ... upregulated in hES cells only, node C contained the genes upregulated in EB only, and node D contained the genes that were upregulated in adult cells HMVEC = human microvascular endothelial cells, ... greater in hES cells than in EB cells and it was not even detected in adult cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... defects in humans [5,6] However, to date, the delivery of MSCs into cartilaginous lesions has neither clinically nor experimentally resulted in sustained regeneration of hyaline cartilage in vivo ... adenoviral delivery of individual cDNAs encoding BMP-2 or TGF-β1 into primary MSCs is capable of driving chondrogenesis in culture [19,20] In the present study, using adenoviralmediated gene transfer ... resuspended in complete DMEM (containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin), and plated at to × 107 nucleated cells per 75 cm2 flask (Falcon, Beckton Dickinson Labware, Franklin...
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Báo cáo khoa học: SOX10, in combination with Sp1, regulates the endothelin receptor type B gene in human melanocyte lineage cells pptx
... higher in HMV-II cells than that in HeLa cells This observation was also seen in normal human epidermal melanocytes, and 624mel human melanoma cell lines examined (Fig 3B), and other human melanoma ... simultaneous binding of the two proteins to the GC1 ⁄ CA2 SOX10 and Sp1 bind to the EDNRB promoter in vivo To investigate whether SOX10 and ⁄ or Sp1 bind to the EDNRB gene in vivo, chromatin immunoprecipitaion ... binding of SOX10 and Sp1 proteins to the GC1 ⁄ CA2, but were unable to detect the complexes, containing both SOX10 and Sp1 proteins (data not shown) It is conceivable that the in vitro binding...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... Translational Medicine 2008, 6:54 cells, the number (cells/ mm2/mouse) of single stranded DNA (ss-DNA)+ cells in one field in the ischemic core from each mouse in the saline- or hES-ECs+MCs-injected group ... human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of VEGF-R2, VE-cadherin and TRA-1 during the ... neuroprotection in the ischemic brain human embryonic stem cells diferentiationon OP9 feeder Day 10 Day VEGF-R2(+) / VE-cadherin(+) / TRA-1(-) cells VEGF-R2(+) / VE-cadherin (-) / TRA-1 (-) cells expansion...
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Báo cáo khoa hoc:" Human haematopoietic stem cells express Oct4 pseudogenes and lack the ability to initiate Oct4 promoter-driven gene expression" ppt
... particularly during carcinogenesis [31,18,19] Kandouz et al [18] revealed that a Connexin43 pseudogene [ψCx43] plays a functional role in some tumour cells The protein Connexin43, involved in intercellular ... cell lines HepG2 and Hek203T In summary our results suggest that Oct4 does not appear to be involved in adult stem cell multipotency, in human UCB progenitor cells In support of our findings, ... stem cells, using Oct4 gene ablation They show that Oct4 is dispensable for self-renewal in intestinal epithelium, bone marrow mesenchymal stem cells and HSC, hair follicle, brain and liver in...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein ... Belmonte JC: Methods for making induced pluripotent stem cells: reprogramming a la carte Nat Rev Genet 2011, 12:231-242 Takahashi K, Yamanaka S: Induction of pluripotent stem cells from mouse embryonic ... signatures Cell Stem Cell 2009, 5:111-123 93 Chin MH, Pellegrini M, Plath K, Lowry WE: Molecular analyses of human induced pluripotent stem cells and embryonic stem cells Cell Stem Cell 2010,...
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Human pluripotent stem cell derived cellular vehicles for cancer gene therapy
... by applying pluripotent stem cells including embryonic stem cells (Chapter 3) and induced pluripotent stem cells (Chapter 4) in the combination with gene delivery The tasks of these investigations ... Pluripotent stem cells 19 20 20 1.2.1.1.1 Embryonic stem cells 20 1.2.1.1.2 Induced pluripotent stem cells 21 1.2.1.2 Adult stem cells 23 1.2.1.2.1 Mesenchymal stem cells 23 1.2.1.2.2 Neural stem cells ... that contaminated cells are completely removed 1.2.1.1.2 Induced pluripotent stem cells Induced pluripotent stem cells (iPSCs) were first generated in the Takahashi and Yamanaka lab in 2006 by...
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Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1
... fetal mesenchymal stem cells through paracrine signalling mechanisms in vitro and induces neovasculogenesis in vivo prior to bone repair Tissue Engineering and Regenerative Medicine International ... Carmeliet and Jain (Carmeliet and Jain 2000) (B) Sprouting of the endothelial cells is induced, resulting in tip cells migrating towards the hypoxic stimulus, with stalk cells following behind New vascular ... J Chan Human endothelial progenitor cells & bone marrow-derived human fetal mesenchymal stem cells potentiate osteogenesis via paracrine activity & induce neovasculogenesis in tissue engineered...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... differentiation of human mesenchymal stem cells on laminin-5 J Cell Biochem (2):499-514 102 Lozito TP, Kuo CK, Taboas JM, et al 2009 ;Human mesenchymal stem cells express vascular cell phenotypes upon interaction ... colony-forming cell and the haemopoietic stem cell Blood Cells (12):7-25 32 Moore KA, Lemischka IR 2006 ;Stem cells and their niches Science (5769):1880-1885 33 Lemischka IR, Moore KA 2003 ;Stem cells: interactive...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
... staining (b) H1 von Kossa staining (c) hFOB H & E staining (d) hFOB von Kossa staining Figure At 4-week time point: H&E staining and von Kossa staining of sections showing scaffolds devoid of cells ... hFOB H & E staining (d) hFOB von Kossa staining Scaffold (e) hFOB H & E staining Figure At 2-week time point: H&E staining and von Kossa staining of sections showing scaffold and cells (e) hFOB ... time-point: (a) (c) nuclear staining with DAPI (b) Immunostaining with bone specific ALP (arrow showing the cells positive for ALP stain) (d) Immunostaining with osteonectin (arrow showing cells...
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Human embryonic stem cells as a cellular model for osteogenesis in implant testing and drug discovery
... brings in new hope in stem cell research especially in generating stem cells without any viral factors involved It also eases concerns on the safety use of induced pluripotent stem cells in clinical ... deriving new cell lines using embryo The third way is through genetic manipulation of somatic cells to creat iPSCs 1.2.2.3 Induced pluripotent stem (iPS) cells: Induced pluripotent stem cells, ... specialized somatic cells and maintaining normal turnover of regenerative organs such as blood, skin and intestinal tissues 1.2.1 Significance in the use of stem cells: Through research into human growth...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... of mitomycin C-inactivated murine embryonic fibroblast feeder (MEF) cells [7, 8] These were harvested from CF1 inbred mouse strain purchased from Charles River Laboratories Inc (Wilmington, MA, ... pre-equilibrated culture media [15, 16] Indeed, minimizing low temperature exposure of human embryos and oocytes is a critical factor in determining the success of clinical assisted reproduction [15, ... analyzed using ISIS mBAND/mFISH imaging software (MetaSystems GmbH, Altlussheim, Germany) In the mFISH technique, each chromosome (1–22 and X and Y) is painted a different color, using combinatorial...
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Tài liệu Báo cáo khoa học: Transcriptional upregulation of inflammatory cytokines in human intestinal epithelial cells following Vibrio cholerae infection pptx
... release of cytokines upon V cholerae infection in intestinal epithelial cells Reports have shown the induction of IL-8 in intestinal epithelial cells upon V cholerae infection [11–13] The induction ... Cytokine response in infected epithelial cells A Bandyopadhaya et al Fig Induction of cytokine expression in intestinal epithelial cell lines by V cholerae (A) Int407 and T84 cells were infected ... RT-PCR in Int407 and T84 cells showed that all the proinflammatory cytokines, except IL-1a and IL-8, were induced to a greater extent in Int407 cells than in T84 cells upon V cholerae infection...
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Báo cáo khoa học: Identification of carbonic anhydrase 9 as a contributor to pingyangmycin-induced drug resistance in human tongue cancer cells ppt
... containing Hepatocellular carcinoma-associated protein HCA10 Chromosome 20 ORF 30 Splicing factor proline ⁄ glutamine rich (polypyrimidine tract-binding protein associated) Metallothionein 1B ... protein), c11 Polyglutamine-binding protein FK506-binding protein Phosphoserine phosphatase-like Chromosome 15 ORF 24 Coiled-coil domain-containing protein 72 Dihydropyrimidinase-like ADP-ribosylation ... Bone morphogenetic protein Poly(A)-specific ribonuclease LY6 ⁄ PLAUR domain containing Inositol 1,4,5-trisphosphate receptor, type Metallothionein 2A Integrin a5 precursor Nucleolar protein 5A (56...
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