factor analysis for the cbi and rutter apos s scale
Báo cáo y học: " Psychopathological status, behavior problems, and family adjustment of Kuwaiti children whose fathers were involved in the first gulf war" ppsx
... Father 's anxiety Father 's PTSD severity Mother 's depression Father 's LOC pre war Mother 's anxiety Father 's military status Father 's PTSD severity Mother 's anxiety Father 's depression Father 's anxiety ... assistants for their professionalism in interviewing the subjects We thank the soldiers and their families for their kindness and patience in welcoming us to their homes and cooperating for the ... problems, etc The subscale score is 0–10 (iii) Statements on behavior (18 items): e.g., restless, destroys own or others' belongings, fights with others, has twitches, mannerisms or tics, sucks thumb...
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Tài liệu Báo cáo khoa học: Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity ppt
... Coomassie blue stained SDS ⁄ PAGE showing the purified GST-fusion proteins used in this study The order corresponds to lanes 2–17 above S( Q420A), GST-ExoS(G421A), GST-ExoS(L422A), GST-ExoS(L423A), ... This This This This This This FEBS Journal 273 (2006) 638–646 ª 2006 The Authors Journal compilation ª 2006 FEBS study study study study study study study study study study study study study study ... 14-3-3 and subsequently for their cytotoxicity and ADP-ribosylation potential using Ras as a substrate in vivo We show that the leucine residue at position 428 is necessary for both the ADP-ribosylation...
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Tài liệu Báo cáo khoa học: Inhibitory effects of nontoxic protein volvatoxin A1 on pore-forming cardiotoxic protein volvatoxin A2 by interaction with amphipathic a-helix doc
... which GSP-3 was used along with the sense primer AP-2 to obtain the 5¢-end of the VVA1 cDNA The products of the second PCR were used to design GSP-2 and GSP-4 specific sense primers corresponding ... corresponds to amino acid residues 163–168 of VVA1 The products of this second PCR were sequenced and used to design the specific antisense primers GSP-1, corresponding to amino acid residues 40–47 ... arrows represent b-strands, and the rods represent a-helices Secondary structural elements of VVA1-NTD with PROF scores below are shown in light orange The completely conserved residues are shaded...
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Tài liệu Báo cáo khoa học: Major phosphorylation of SF1 on adjacent Ser-Pro motifs enhances interaction with U2AF65 doc
... phosphorylation and by KIS Experimental procedures Plasmids and mutagenesis The plasmid for expression of the human SF1 fragment, SF1f (residues 1–255) was constructed in the pGEX6P-1 vector (Amersham Biosciences, ... trypsin, as previously observed by MS, and to the presence of single and double phosphorylation of the peptides as suggested by our analysis of SF1f phosphorylated by KIS to different extents in vitro ... of KIS, compared with other kinases and phosphatases, for regulating the phosphorylation state of the SF1 SPSP motif in vivo is an intriguing subject for further investigation By comparing the...
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Tài liệu Báo cáo khoa học: Plant a-amylase inhibitors and their interaction with insect a-amylases ppt
... also inactive against different classes of proteinases such as trypsins and chymotrypsins [53] BASI is deposited during grain ®lling and therefore found in the seed prior to AMY2, which is synthesized ... acarviosine-glucose, hibiscus acid and the cyclodextrins (Fig 2A) The two hibiscus acid forms, puri®ed from Roselle tea (Hibiscus sabdaria), the acarviosine-glucose, the isoacarbose and a-, b- and ... sources In these cases, an improved understanding of the structural bases for inhibition pro®les (as discussed later) may enable the rational design of mutants with more desirable characteristics...
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Tài liệu MECHANICAL PROPERTIES OF THE HEART AND ITS INTERACTION WITH THE VASCULAR SYSTEM ppt
... arteries, the pulmonary capillaries and pulmonary veins and the systemic vascular system which contains the left ventricle, the systemic arteries, the systemic capillaries and the systemic veins Each ... ventricular pressure just exceeds aortic pressure; this pressure therefore reflects the pressure existing in the aorta at the onset of ejection and is called the diastolic blood Page 11 of 23 pressure ... During the ejection phase, aortic and ventricular pressures are essentially equal; therefore, the point of greatest pressure on the loop also represents the greatest pressure in the aorta, and this...
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Báo cáo khoa học: Peptides from purified soybean b-conglycinin inhibit fatty acid synthase by interaction with the thioesterase catalytic domain pot
... doses as low as 12 lm To compare the potency of the FAS inhibitory response across all of the peptides and the C75 positive control, classical sigmoidal dose–response curves were plotted and used ... acid sequences were custom synthesized and their FAS inhibitory activities tested Figure shows the FAS inhibitory activity of the three synthetic peptides and the C75 positive control The FAS inhibitory ... and 35 lm NADPH and 0.3 lm FAS in 0.1 m potassium phosphate buffer Initial rates were calculated for the slope of the progress curves during the first FAS inhibition studies Synthetic peptides...
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Báo cáo khoa học: Ion-binding properties of Calnuc, Ca2+ versus Mg2+ – Calnuc adopts additional and unusual Ca2+-binding sites upon interaction with G-protein pdf
... based on the two isoforms of Calnuc present in them A phylogram, obtained from all the sequences, clusters lower organisms and mammals as separate groups The tree consists of two clades, representing ... parvalbumin, the J-band is simply lost and the bound dye is released In the case of crystallins (eye lens proteins), it has been shown that, whereas b-crystallin generates only the J-band, d-crystallin ... organism [40] (also see Table S1 for comparison of the Calnuc EF-hand sequence with the consensus sequence) The region between the two EF-hands, which is known to bind Gia, seems to be highly conserved...
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Báo cáo khoa học: SHIP2 interaction with the cytoskeletal protein Vinexin ppt
... we also compared the effect of SHIP2 with another construct that did not have the SHIP2 SH2 domain (DSH2 SHIP2) Cells expressing these constructs were detached from their culture dishes and then ... SHIP2 in transfected COS-7 cells The association of Vinexin a and SHIP2 was examined in COS-7 cells Vinexin a (the longest isoform) was chosen rather than Vinexin b because the construct 6054 ... attached and unattached cells on the dish were counted The number of attached cells was expressed as in Fig Data are expressed as means ± SEM (n ¼ 3; *P < 0.05) PtdIns(4,5)P2 were used as substrates...
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Báo cáo khoa học: tmRNA from Thermus thermophilus Interaction with alanyl-tRNA synthetase and elongation factor Tu pptx
... does not limit measurements by the initial stages of the reaction and allows to include into the analysis datapoints from other parts of the reaction progress curve On the other hand, the successful ... At the same time, alanyl-tRNA synthetase possesses slightly higher affinity towards tmRNA, mostly because of slower dissociation of the AlaRSase*tmRNA complex in comparison with the AlaRSase*tRNAAla ... hydrolytic decay was performed with the use of the ÔLSW Data Analysis ToolboxÕ add-in (MDL Information Systems, Inc) for Microsoft EXCEL Results The sequence of the ssrA gene of T thermophilus HB8 determined...
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Báo cáo khoa học: Concerted mutation of Phe residues belonging to the b-dystroglycan ectodomain strongly inhibits the interaction with a-dystroglycan in vitro pot
... plays an essential role as a scaffold for cells in muscle and in a wide variety of nonmuscle tissues [6,7], including the central and peripheral nervous systems, and several epithelial tissues ... with dystrophin, utrophin and other cytosolic proteins, such as rapsyn, caveolin-3 and Grb2 [3–5] Together with sarcoglycans, dystrobrevins, syntrophins, and sarcospan, DG forms the dystrophin–glycoprotein ... sitedirected mutations We performed amino acid substitutions using alanine, because this residue does not show any propensity for a specific secondary structure, and therefore does not perturb the overall...
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Báo cáo khoa học: Intermonomer cross-linking of F-actin alters the dynamics of its interaction with H-meromyosin in the weak-binding state ppt
... to Cys374 [12] among actin molecules within the same strand, Cross-linking affects the dynamics of F-actin and another cross-link between Cys374 to Lys191 [13] connects opposing strands in the ... from six monomers is shown with different colours using PDB 1MVW data Yellow and red spheres are assigned Cys374 and Gln41, respectively Black lines show cross-links between Gln41 and Cys374 residues ... Lys191–Cys374 cross-links [13], but on the basis of the positive cooperation of the cross-link formation between Gln41 and Lys113 some structural change was considered [15] For this reason, we...
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Báo cáo khoa học: Identification of amino acids in antiplasmin involved in its noncovalent ‘lysine-binding-site’-dependent interaction with plasmin pptx
... express wt-antiplasmin was established Mutagenesis of antiplasmin The cDNA of wt-antiplasmin, subcloned into the expression vector pMT/Bip/V5 at the BglII and XhoI sites, was used to produce the selected ... measured, and rate constants were calculated assuming pseudo-first-order kinetics The rate constants for the reactions between plasmin and the antiplasmin variants are shown in Table (in both the presence ... wt-antiplasmin and seven antiplasmin mutants were expressed in Drosophila S2 cells With the expression plasmid used (see Material and methods), the antiplasmin variants were exported via the endoplasmic reticulum...
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Báo cáo khoa học: Amino acid residues on the surface of soybean 4-kDa peptide involved in the interaction with its binding protein potx
... methods Phases before the asterisk (*) represent the association sensorgrams; phases after the asterisk represent the dissociation sensorgrams The kinetic parameters, association rate constant ... ( 3S S) E11A ( 3S S) V12A ( 3S S) R16A ( 3S S) R18A ( 3S S) D19A ( 3S S) R21A ( 3S S) V23A ( 3S S) I25A ( 3S S) L27A ( 3S S) F28A ( 3S S) V29A ( 3S S) F31A ( 3S S) I33A ( 3S S) H34A ( 3S S) T36A ( 3S S) 3916.72 ... Phases before the asterisk (*) represent the association sensorgrams; phases after the asterisk represent the dissociation sensorgrams Ó FEBS 2003 2588 K Hanada et al (Eur J Biochem 270) disulfide...
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Báo cáo khoa học: Crystal structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor potx
... as shown for SGRSGRSG-RATE) [16] GPPG-RATE, SGSGSG-RATE and SGRSGRSGRATE were successfully crystallized as described in Experimental procedures, all yielding the same crystal form The crystals ... RI and RATEs, the crystal structures of various RATEs differing in linker sequence length and amino acid composition (GPPG, SGSGSG, or SGRSGRSG) were solved On the basis of these structures, ... Table S1 Crystallographic data processing and refinement statistics for the RATEs with the linker sequences GPPG, SGSGSG, and SGRSGRSG This supplementary material can be found in the online version...
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Báo cáo khoa học: A L225A substitution in the human tumour suppressor HIC1 abolishes its interaction with the corepressor CtBP docx
... HDACs [30] When these experiments were performed in the presence of TSA, a specific inhibitor of class I and class II HDACs, the repression exhibited 2884 both by the wt and the L225A chimeras was ... autonomous repression domain Thus, the HIC1 CR represses transcription through CtBP-dependent and CtBP-independent mechanisms and involves the recruitment of class I or class II HDACs Discussion ... Using the HIC1 GxDLS motif as a paradigm for these nonconsensus motifs, we investigated the properties of its binding to CtBPs In their elegant work on the crystal structure of rat CtBP ⁄ BARS...
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Báo cáo khoa học: Contribution of a central proline in model amphipathic a-helical peptides to self-association, interaction with phospholipids, and antimicrobial mode of action ppt
... measurements from three independent assays Table Amino-acid sequences and molecular masses of the model peptides Observed mass was from Kratos Kompact MALDI TOF MS Structural analysis of the ... constants and affinity constants obtained from the two-state model analysis are listed in Table There were striking differences between PFPs and PCPs in the association rate (ka1) for first step and ... R1 and R2 are the response units for the first and second steps, respectively, CA is the peptide concentration, 4052 and Rmax is the maximal response unit (or equilibrium binding response) The...
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Báo cáo khóa học: Identification of domains on the extrinsic 23 kDa protein possibly involved in electrostatic interaction with the extrinsic 33 kDa protein in spinach photosystem II ppt
... and Lys14; one Lys among Lys27 and Lys38; one Lys at Lys40; one Lys at Lys90 or Lys96; one Lys at Lys143 or Lys152; two Lys between Lys166 and Lys174 (Table 2) These modified lysyl residues were ... propose that the candidates for electrostatic interaction of the 23 kDa protein with the 33 kDa protein associated with the PSII membranes are at least present in lysyl residues of the six domains ... least some of the positive charges of the lysyl residues in the six domains of the 23 kDa protein interact electrostatically with these negative charges of the 33 kDa protein Some of the positive...
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Báo cáo khoa học: Aptamers toEscherichia colicore RNA polymerase that sense its interaction with rifampicin, r-subunit and GreB ppt
... strength Discussion The principal result of this work is that the aptamers sense the interaction of RNAP with various ligands, including nucleic acids, antibiotics and protein factors Based on the mechanism ... the dissociation kinetics of the RNAP–aptamer complexes measured in the presence of MS and Rif followed the same time course as the kinetics measured in the presence of the unlabeled aptamers ... change if the measurements were done in the absence of the inhibitors Minimal nucleic acid scaffold (MS) The sequences of DNA and RNA oligos used to reconstitute MS are shown in Fig 1A MS was prepared...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25