... indicated by their amino acid substitution, were reacted with uPA (200 lgÆmL)1) at 37 °C in HBS, pH 7.4 for 30 and the products were separated by nonreducing SDS/PAGE (11% acrylamide) followed by staining ... Mutagenesis Kit (Stratagene) as described by the manufacturer, except that the final product was electroporated into E coli DH5a cells, and confirmed by sequencing using the Thermo Sequenase II ... HBS at 37 °C for 30 and quenched by boiling in SDS sample buffer The reaction products were subjected to nonreducing SDS/PAGE in 11% acrylamide gels followed by staining with Coomassie brilliant...
... generated by digestion of RNase A by subtilisin Carlsberg only Assigned RNase A sequence Molecular mass determined by MALDI-MS (Da) Suggested RNase fragment Fraction N-Terminal sequence determined by ... followed by fluorescence and CD spectroscopy, activity measurements and proteolysis fN represents the fraction of native protein as determined by fluorescence spectroscopy at 303 nm (s) or by CD spectroscopy ... trifluoroethanol (40%), samples were analysed by RP-HPLC, MALDI-MS and N-terminal protein sequencing A typical RP-HPLC chromatogram for the proteolytic digestion of RNase A by subtilisin Carlsberg is shown...
... sets of trees derived by IG's (like those of TAG's) are context-free languages Trees derived by IG's exhibit a property that is not exhibited by the trees sets derived by TAG's or CFG's Informally, ... is bounded by the grammar (in fact the maximum cardinality of a tree set determines this bound) Hence, trees shown in Figure can not be generated by any MCTAG (but can be generated by an IG) because ... Although this property is not structural, it depends on the structural property that sentences can be built from a finite set of clauses of bounded structure as noted by Joshi (1983/85) The property...
... Linear Complementarity Problem FRC = Fibre ReinforcedConcrete SFRC = Steel Fibre ReinforcedConcrete NSC = Normal Strength Concrete HSC = High Strength Concrete TPB = Three-Point Bending FEM = ... The safety and durability of concrete structures are significantly influenced by the fracture behaviour of the concrete There are many fracture formulations which assume concrete as a homogeneous ... microstructural analysis of concrete (Vonk, 1992; Stankowski et al., 1993), mortar joints in masonry structures (Lotfi and Shing, 1994), fibre -reinforced composite interfaces (Weihe et al., 1994), concrete- steel...
... number based on rank by length, and is preceded by a hash mark (e.g #1gag) The position of some mIMRs differ by only a few amino acids, so it is possible to simplify the data by discounting mIMRs ... coincide with key structural domains [1] Another type of mirror repeat is identified by progressively evaluating, from the start to the end of a sequence, symmetric sub-strings bounded by reverse dinucleotides ... are identified by sequentially evaluating, from 5' to 3', the symmetry of each substring delineated by each dinucleotide and the next downstream reverse dinucleotide They are nested by the same...
... materialized Franỗois Axisa August 2003 Introduction To understand what is meant bystructural elements, it is convenient to start by considering a whole structure made of various components assembled together ... importance to optimise the mechanical resistance of structuralelements is the curvature of the equivalent solid Based on these two properties structuralelements can be identified as: Straight beams, ... these two features in structural modelling is by far not a simple task can be amply asserted by recalling that it progressed, along with the necessary mathematics, step by step over a long period...
... materialized Franỗois Axisa August 2003 Introduction To understand what is meant bystructural elements, it is convenient to start by considering a whole structure made of various components assembled together ... importance to optimise the mechanical resistance of structuralelements is the curvature of the equivalent solid Based on these two properties structuralelements can be identified as: Straight beams, ... these two features in structural modelling is by far not a simple task can be amply asserted by recalling that it progressed, along with the necessary mathematics, step by step over a long period...
... fy edit box • Verify is entered in the Concrete Strength, fc edit box • Type 60 in the Shear steel Yield Stress, fys edit box • Verify is entered in the Concrete Shear Strength, fcs edit box • ... ä ä In the Element Class area select the Beam option In the Concrete Cover To Rebar Center area type 3.5 in the Top edit box In the Concrete Cover To Rebar Center area type 2.5 in the Bottom edit ... close the Analysis window 29 On the Design menu verify that the Concrete Design feature is active There should be a check mark next Concrete Design if it is active 30 From the Design menu click...
... shear strength contribution byconcrete shear resisting mechanism and Vs is the shear strength contribution by the truss mechanism provided by shear reinforcement Concrete shear contribution, ... bending must be used By using the equilibrium condition that the applied axial load is balanced by the resultant axial forces of concrete and reinforcement, the depth of the concrete compression ... for ideal elastoplastic materials In design, the actual behavior of reinforcedconcretestructural components is approximated by an idealized bilinear relationship, as shown in Figure 38.2 In such...
... Forest Ranges Kavalčanky, Samčanka and Bílá, Forest District Ostravice, which were heavily damagedby snow in winter 2005–2006 (windfalls and snowbreaks) Our analysis was concentrated only on ... procedures used by Kula and Ząbecki (1996) The group of analyzed trees (275) with breaks at the stem base (39), in the crown (113) and under the crown (123) is characterized by the mean original ... the phloem of breaks Qualitative changes in the phloem on standing breaks were affected by their height and by the presence of living branches In mid-August, 58% of the sections of crown breaks...
... METHODOLOGY RESEARCH RESULTS CONCLUSION General Overview of reinforcedconcrete corrosion under the effect of chloride ion The reinforcedconcrete structure is corroded very fast under the effect ... period until corrosion appeared In the samples with same concrete grade, the steel corrosion is increasing by the time and by Cl- content; Higher concrete grade, lower corrosion Effective content of ... are often used Calcium nitrite (CN) is used as the most effective inhibitors in preventing reinforcedconcrete from corrosion General The inhibition mechanism of CN A Oxidation of FeO to form...
... benefits of high performance concrete HIGH PERFORMANCE CONCRETESTRUCTURAL DESIGNERS’ GUIDE by the High Performance Concrete Technology Delivery Team HIGH PERFORMANCE CONCRETESTRUCTURAL DESIGNER’S ... this risk factor by specifying only the minimum concrete strengths required by the design and by communicating with the fabricators early on in the design When all three cost elements are summed ... three basic cost elements of a concrete structure are materials, labor, and markup Each cost element is affected when HPC is used The primary materials in a concrete structure are concrete, prestressing...
... affect on reinforcedconcrete structures, especially concrete bridges 1.10.2 Service life of reinforcedconcrete bridges at the coastal areas in Vietnam The service life of the reinforcedconcrete ... and CO2 into concrete are expected to significantly enhance the durability and service life of concrete bridges The causes of damages in concrete bridges were statistically presented by professor ... two-stage model of the service life of reinforcedconcrete structures, which considered D as a constant of time 1.2.6 Figure 1.14: Service life of reinforcedconcrete structures: Two-stage model...