... fractions on blood fasting glucose and plasma insulin secretion in obese-diabetic mice Glucose (mmol/l) Plasma insulin (pmol/l) Treatment with extract fraction Starting point Final Change of ... STZ induced HFD fed mice were expressed diabetic disease and a insulin resistance. However, plasma insulin in HFD-fed mice injected STZ was lower as compared to HFD mice without STZ injection. ... mice). While, HDLc in HFD mice decreased 34.63% in comparision with the control (ND mice). Moreover, blood glucose and insulin concentration unusually increase in obese mice in comparison with...
... the crystal packing could be maintained [18].Other methods are available that can provide struc-tural information on these proteins in solution. CDstudies of a-chymotrypsin in organic solvents ... protein aggregation, played an important role in the protein function. Our approach to the study ofprotein structure and dynamics in such media has beenfocused on understanding how proteins ... completeunderstanding of the structural properties of thisenzyme in organic solvents is shown in Fig. 1. Cu-tinase was simulated in five different organic solventsof increasing dielectric constant...
... synthase. The increased activities of argininosuccinate synthetase and argininosuccinate lyase suggest the increased and effective recycling of citrulline to arginine in anoxia, making nitric ... in brain in anoxia as observed by in- crease in NOx concentration in this study support the earlier findings of NO involvement in pathophysiol-ogy of hypobaric hypoxia in brain (42-43). In this ... arginine at the site of synthesis. Arginine is a semi essential amino acid and in CNS, its availability depends upon: (a) uptake from the circu-lating arginine and (b) recycling of citrulline...
... should be careful in negotiating and monitoring as well as learning in forming the joint ventures. As it is a common situation in Vietnam that almost joint ventures are going to be wholly ... procedures, individual batch or history records, and written schedules and procedures for cleaning and maintaining equipment.3. Packaging and Labeling Controls. Since packaging and labeling are ... pharmaceutical manufacturers in Vietnam, in terms of successes in doing business and goodwill. Recognizing the importance and benefits of product’s quality, since September 1996, the Vietnamese Ministry...
... purification.The tagged protein had a similar activity in crude yeastextract as the untagged protein, indicating that the tagwas not interfering with the protein activity. Thetagged protein was purified ... reading frame, and then expressed in S. cerevisiae. The tagged protein had a similar activ-ity in the crude extract as the nontagged protein, indi-cating that the tag did not affect the protein ... dl-glyceraldehyde in an extractof H. jecorina was about 3 nkatÆmg)1. The GLD1 pro-tein was tagged with a histidine tag at the N-terminalend by adding the coding sequence for six histidines tothe...
... arrows). (C–E) Staining of sPLA2-V in three active RA tissues. In all cases, intense staining of the granulation tissue in the sublining interstitium was evident. Staining of the granulation tissue ... v) bovine serum albumin (for blocking) containing0.1% (w ⁄ v) saponin (for permeabilization) in NaCl ⁄ Pifor1 h, with anti-sPLA2and anti-COX-2 1 h in NaCl ⁄ Picon-taining 1% albumin (1 ... sublining region showedintense staining (Cd, orange arrowheads). Although cartilage chondrocytes were negative, fibroblasts in ltrating into the cartilage wereintensely stained (Eb).sPLA2s in...
... low [19]. In this paper, weaddress the following questions: (a) can coupled fumaraterespiration be restored by incorporating the isolated enzymes into liposomes containing menaquinone; (b) ... criticalratio. In the experiment shown in Fig. 2, fumarate reductaseand hydrogenase isolated from W. succinogenes wereincorporated into sonic liposomes containing MK accord-ing to the method ... anasparagine (N110) and a glutamine residue (Q113) withinthe hydrophilic stretch c onnecting helices II and I II. Thesethree residues are conserved in HydC (H200, N128, andQ131 in Fig. 5) and in...
... theamino group of the main chain at Glu268. The oxygen ofthe main chain at Asp266 was bound to amino groups ofmain chain of Tyr269 and Ala270 by hydrogen bonds. Theinteractions should maintain ... in variousindustrial fields. In particular, subtilisins, serine proteasesfrom a variety of Bacillus species,aresomeofthemostinvestigated enzymes [1,2]. Subtilisins are classified intothree ... The alkaline subtilisin group contains the enzymes from alkalophilic Bacillus such as subtilisin YaB[8], no. 221 protease [9], Savinase [10], subtilisin Sendai(Sendai) [11]. Subtilisin ALP I...
... creatine kinase in the same malignant cells do not appear tobe sufficient for the building up of an effective creatine ⁄ phosphocreatinepool. Instead of supporting creatine biosynthesis, l-arginine:glycine ... of different enzymes in the metabolic cycle of arginine and methionine furtherstrengthens this view.Estrogen administration increases the expression ofAGAT in chick liver, indicating that AGAT ... unambiguously demonstrates the prominent expressionof the creatine-synthesizing enzymes l-arginine:glycine amidinotransferaseand N-guanidinoacetate methyltransferase in sarcoma, Ehrlich ascites carci-noma...
... understanding of their intrinstic heatingand denaturing resistance. Elucidating the stabilizingmechanisms has been one of the greatest challenges in biochemistry and biotechnology [3–5].This minireview ... essen-tial roles in proteins, modulating protein stability, folding and function.The dominant roles of the buried polar contacts and ion pairs have beenreviewed by distinguishing between the inside polar ... that cooperative interaction within the hydro-phobic protein core plays an integral role in increasingthe thermalstability. Another potential stabilizing fac-tor is the high packing density of...
... (S)-cheilanthifoline, not (S)-nandinine[7,30,31]. If we assume that (S)-nandinine is produced in E. californica, there may be no need for a catalystfrom (S)-nandinine to (S)-stylopine. However, nandi-nine ... for a variety of isoquinoline alkaloids. (S)-Scoulerine is an intermediate at the branch point leading to benzophe-nanthridine alkaloids in E. californica or berberine in C. japonica. The pathway ... reportedto increase in response to MeJA together with thoseof other P450s involved in sanguinarine biosynthesis in EBDACFig. 8. MeJA induces the expression of CYP719A2 and CYP719A3 genes in E....
... made; in the last wash, theresin containing the recombinant proteins was loaded intoa gravity-flow column. The column was washed once withlysis buffer containing 10 mm imidazole. The proteins ... time, the kinetic char-acterization of recombinant glycolytic enzymes involved in the pathway from glucose to pyruvate in E. histolytica. According to their catalytic efficiencies,several enzymes ... buffer containing 100 mm imidazole. Thepurified proteins were stored in 50% (v ⁄ v) glycerol at)20 °Cor4°C, or in 3.2 m ammonium sulfate at 4 °C.Protein determination was carried out according to...
... be integrated mechanisms.These findings indicate a divergence in the molecular designscontrolling trehalose metabolism in S. pombe from those in S. cerevisiae.MATERIALS AND METHODSStrains ... trehalase and proteinsinvolved in synthesis of trehalose, thus revealing a novelrelationship in trehalose metabolism. In E. coli, trehalases are monomeric enzymes [26].However, in S. cerevisiae ... SpainTrehalose metabolism is an essential component of the stressresponse in yeast cells. In this work we show that the prod-ucts of the principal genes involved in trehalose metabolismin...