0

enzymes in manufacture

Tài liệu Báo cáo

Tài liệu Báo cáo " Effect of Sweet potato (Ipomoea batatas (L.) Lam) leaf extract on hypoglycaemia, blood insulin secretion, and key carbohydrate metabolic enzymes in expermentally obese and STZ-induced diabetic mice " pptx

Báo cáo khoa học

... fractions on blood fasting glucose and plasma insulin secretion in obese-diabetic mice Glucose (mmol/l) Plasma insulin (pmol/l) Treatment with extract fraction Starting point Final Change of ... STZ induced HFD fed mice were expressed diabetic disease and a insulin resistance. However, plasma insulin in HFD-fed mice injected STZ was lower as compared to HFD mice without STZ injection. ... mice). While, HDLc in HFD mice decreased 34.63% in comparision with the control (ND mice). Moreover, blood glucose and insulin concentration unusually increase in obese mice in comparison with...
  • 7
  • 572
  • 0
Báo cáo khoa học: Modeling hydration mechanisms of enzymes in nonpolar and polar organic solvents potx

Báo cáo khoa học: Modeling hydration mechanisms of enzymes in nonpolar and polar organic solvents potx

Báo cáo khoa học

... the crystal packing could be maintained [18].Other methods are available that can provide struc-tural information on these proteins in solution. CDstudies of a-chymotrypsin in organic solvents ... protein aggregation, played an important role in the protein function. Our approach to the study ofprotein structure and dynamics in such media has beenfocused on understanding how proteins ... completeunderstanding of the structural properties of thisenzyme in organic solvents is shown in Fig. 1. Cu-tinase was simulated in five different organic solventsof increasing dielectric constant...
  • 13
  • 433
  • 0
enzymes in industry

enzymes in industry

Sinh học

  • 515
  • 268
  • 0
Báo cáo y học:

Báo cáo y học: "NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN"

Y học thưởng thức

... synthase. The increased activities of argininosuccinate synthetase and argininosuccinate lyase suggest the increased and effective recycling of citrulline to arginine in anoxia, making nitric ... in brain in anoxia as observed by in- crease in NOx concentration in this study support the earlier findings of NO involvement in pathophysiol-ogy of hypobaric hypoxia in brain (42-43). In this ... arginine at the site of synthesis. Arginine is a semi essential amino acid and in CNS, its availability depends upon: (a) uptake from the circu-lating arginine and (b) recycling of citrulline...
  • 8
  • 622
  • 0
The challenges for implementation of good manufacturing practices by local pharmaceutical manufactures in vietnam

The challenges for implementation of good manufacturing practices by local pharmaceutical manufactures in vietnam

Thạc sĩ - Cao học

... should be careful in negotiating and monitoring as well as learning in forming the joint ventures. As it is a common situation in Vietnam that almost joint ventures are going to be wholly ... procedures, individual batch or history records, and written schedules and procedures for cleaning and maintaining equipment.3. Packaging and Labeling Controls. Since packaging and labeling are ... pharmaceutical manufacturers in Vietnam, in terms of successes in doing business and goodwill. Recognizing the importance and benefits of product’s quality, since September 1996, the Vietnamese Ministry...
  • 72
  • 932
  • 2
Tài liệu Báo cáo khoa học: Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina doc

Tài liệu Báo cáo khoa học: Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina doc

Báo cáo khoa học

... purification.The tagged protein had a similar activity in crude yeastextract as the untagged protein, indicating that the tagwas not interfering with the protein activity. Thetagged protein was purified ... reading frame, and then expressed in S. cerevisiae. The tagged protein had a similar activ-ity in the crude extract as the nontagged protein, indi-cating that the tag did not affect the protein ... dl-glyceraldehyde in an extractof H. jecorina was about 3 nkatÆmg)1. The GLD1 pro-tein was tagged with a histidine tag at the N-terminalend by adding the coding sequence for six histidines tothe...
  • 7
  • 510
  • 0
Tài liệu Báo cáo khoa học: Various secretory phospholipase A2 enzymes are expressed in rheumatoid arthritis and augment prostaglandin production in cultured synovial cells docx

Tài liệu Báo cáo khoa học: Various secretory phospholipase A2 enzymes are expressed in rheumatoid arthritis and augment prostaglandin production in cultured synovial cells docx

Báo cáo khoa học

... arrows). (C–E) Staining of sPLA2-V in three active RA tissues. In all cases, intense staining of the granulation tissue in the sublining interstitium was evident. Staining of the granulation tissue ... v) bovine serum albumin (for blocking) containing0.1% (w ⁄ v) saponin (for permeabilization) in NaCl ⁄ Pifor1 h, with anti-sPLA2and anti-COX-2 1 h in NaCl ⁄ Picon-taining 1% albumin (1 ... sublining region showedintense staining (Cd, orange arrowheads). Although cartilage chondrocytes were negative, fibroblasts in ltrating into the cartilage wereintensely stained (Eb).sPLA2s in...
  • 18
  • 432
  • 0
Tài liệu Báo cáo khoa học: Reconstitution of coupled fumarate respiration in liposomes by incorporating the electron transport enzymes isolated from Wolinella succinogenes docx

Tài liệu Báo cáo khoa học: Reconstitution of coupled fumarate respiration in liposomes by incorporating the electron transport enzymes isolated from Wolinella succinogenes docx

Báo cáo khoa học

... low [19]. In this paper, weaddress the following questions: (a) can coupled fumaraterespiration be restored by incorporating the isolated enzymes into liposomes containing menaquinone; (b) ... criticalratio. In the experiment shown in Fig. 2, fumarate reductaseand hydrogenase isolated from W. succinogenes wereincorporated into sonic liposomes containing MK accord-ing to the method ... anasparagine (N110) and a glutamine residue (Q113) withinthe hydrophilic stretch c onnecting helices II and I II. Thesethree residues are conserved in HydC (H200, N128, andQ131 in Fig. 5) and in...
  • 10
  • 569
  • 0
Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx

Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx

Báo cáo khoa học

... theamino group of the main chain at Glu268. The oxygen ofthe main chain at Asp266 was bound to amino groups ofmain chain of Tyr269 and Ala270 by hydrogen bonds. Theinteractions should maintain ... in variousindustrial fields. In particular, subtilisins, serine proteasesfrom a variety of Bacillus species,aresomeofthemostinvestigated enzymes [1,2]. Subtilisins are classified intothree ... The alkaline subtilisin group contains the enzymes from alkalophilic Bacillus such as subtilisin YaB[8], no. 221 protease [9], Savinase [10], subtilisin Sendai(Sendai) [11]. Subtilisin ALP I...
  • 9
  • 489
  • 0
Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx

Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx

Báo cáo khoa học

... creatine kinase in the same malignant cells do not appear tobe sufficient for the building up of an effective creatine ⁄ phosphocreatinepool. Instead of supporting creatine biosynthesis, l-arginine:glycine ... of different enzymes in the metabolic cycle of arginine and methionine furtherstrengthens this view.Estrogen administration increases the expression ofAGAT in chick liver, indicating that AGAT ... unambiguously demonstrates the prominent expressionof the creatine-synthesizing enzymes l-arginine:glycine amidinotransferaseand N-guanidinoacetate methyltransferase in sarcoma, Ehrlich ascites carci-noma...
  • 11
  • 626
  • 0
Báo cáo khoa học: Implication for buried polar contacts and ion pairs in hyperthermostable enzymes pot

Báo cáo khoa học: Implication for buried polar contacts and ion pairs in hyperthermostable enzymes pot

Báo cáo khoa học

... understanding of their intrinstic heatingand denaturing resistance. Elucidating the stabilizingmechanisms has been one of the greatest challenges in biochemistry and biotechnology [3–5].This minireview ... essen-tial roles in proteins, modulating protein stability, folding and function.The dominant roles of the buried polar contacts and ion pairs have beenreviewed by distinguishing between the inside polar ... that cooperative interaction within the hydro-phobic protein core plays an integral role in increasingthe thermalstability. Another potential stabilizing fac-tor is the high packing density of...
  • 11
  • 533
  • 0
Báo cáo khoa học: Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica doc

Báo cáo khoa học: Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica doc

Báo cáo khoa học

... (S)-cheilanthifoline, not (S)-nandinine[7,30,31]. If we assume that (S)-nandinine is produced in E. californica, there may be no need for a catalystfrom (S)-nandinine to (S)-stylopine. However, nandi-nine ... for a variety of isoquinoline alkaloids. (S)-Scoulerine is an intermediate at the branch point leading to benzophe-nanthridine alkaloids in E. californica or berberine in C. japonica. The pathway ... reportedto increase in response to MeJA together with thoseof other P450s involved in sanguinarine biosynthesis in EBDACFig. 8. MeJA induces the expression of CYP719A2 and CYP719A3 genes in E....
  • 17
  • 376
  • 0
Báo cáo khoa học: Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and flux control analysis ppt

Báo cáo khoa học: Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and flux control analysis ppt

Báo cáo khoa học

... made; in the last wash, theresin containing the recombinant proteins was loaded intoa gravity-flow column. The column was washed once withlysis buffer containing 10 mm imidazole. The proteins ... time, the kinetic char-acterization of recombinant glycolytic enzymes involved in the pathway from glucose to pyruvate in E. histolytica. According to their catalytic efficiencies,several enzymes ... buffer containing 100 mm imidazole. Thepurified proteins were stored in 50% (v ⁄ v) glycerol at)20 °Cor4°C, or in 3.2 m ammonium sulfate at 4 °C.Protein determination was carried out according to...
  • 17
  • 589
  • 0
Báo cáo Y học: Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the fission yeast Schizosaccharomyces pombe pdf

Báo cáo Y học: Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the fission yeast Schizosaccharomyces pombe pdf

Báo cáo khoa học

... be integrated mechanisms.These findings indicate a divergence in the molecular designscontrolling trehalose metabolism in S. pombe from those in S. cerevisiae.MATERIALS AND METHODSStrains ... trehalase and proteinsinvolved in synthesis of trehalose, thus revealing a novelrelationship in trehalose metabolism. In E. coli, trehalases are monomeric enzymes [26].However, in S. cerevisiae ... SpainTrehalose metabolism is an essential component of the stressresponse in yeast cells. In this work we show that the prod-ucts of the principal genes involved in trehalose metabolismin...
  • 9
  • 428
  • 0

Xem thêm