dna rna and protein extractions
Báo cáo y học: "Inflammatory responses to acute pneumovirus infection in neonatal mice" pps
... by dissection and placed into ml RPMI 1640 with 5% fetal bovine serum (FBS) The lungs were teased and cut into pieces and then digested with mL RPMI with 5% FBS, 20 μg/mL DNAse I and mg/mL collagenase ... First strand cDNA was used for real-time PCR detection of transcripts encoding cytokines, chemokines and related inflammatory mediators and housekeeping genes; controls for genomic DNA contamination, ... containing 0.005 M EDTA and DNase I), then twice in Hanks balanced salt solution Isolated lung cells were counted and stained for flow cytometry using the following antibodies and dilutions (all from...
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Báo cáo sinh học: "Tissue specific promoters improve specificity of AAV9 mediated transgene expression following intra-vascular gene delivery in neonatal mice" pptx
... β-galactosidase enzyme detection assays and drafted the manuscript YS designed and cloned the plasmids and harvested tissues BDT performed the RNA transcript analysis and helped to draft the manuscript ... skeletal and smooth muscle [20] Two different isoforms of MLC-2 are typically expressed in the atria and ventricles of the mammalian heart during embryonic development and maturation, and later ... The α-MHC protein is a "fast" ATPase myosin and is located in the thick filaments of myofibrils It is important for cardiomyocyte contraction and relaxation [18] In mice, the α-MHC protein is...
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báo cáo hóa học: " Boosting with intranasal dendrimeric Aβ1–15 but not Aβ1–15 peptide leads to an effective immune response following a single injection of Aβ1–40/42 in APP-tg mice" ppt
... Aβx-40 and Aβx42 There was a non-significant reduction of Aβ40 and Aβ42 TBS and guanidine soluble levels (commonly referred to as soluble and insoluble fractions) in both the Aβ1–40/42 and dAβ1–15 ... measured in splenocyte supernatants using matching antibody pairs composed of a capture and detection antibody for IL-4 and IFN-γ (BD PharMingen) Aβ ELISA Both soluble and insoluble brain Aβ levels ... isolated and harvested using standard methods as previously reported [32] Aβ peptides were added to cultures in triplicate at a final concentration of 0, 0.5, or 50 µg/ml At 48 and 72 hours, supernatants...
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báo cáo hóa học: " Interleukin-1 receptor 1 knockout has no effect on amyloid deposition in Tg2576 mice and does not alter efficacy following Aβ immunotherapy" pot
... have been identified: type I and type II receptors (IL-RI and IL-RII) [26] IL-1β binds IL-1RI and upon IL-1 binding, IL-1RI recruits the accessory protein IL-1R-AcP, and initiates a stimulatory ... number not for citation purposes) Journal of Neuroinflammation 2006, 3:17 assays, including IL-1-induced IL-6 and E-selectin expression, and IL-1-induced fever and acute-phase responses to turpentine ... to five to a cage and maintained on ad libitum and water with a 12 h light/dark cycle Passive immunizations Groups of APP/IL-1 R1-/- mice and APP/IL-1 R1+/- littermates (males and females, 6-month-old...
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Báo cáo khoa học: "Molecular analysis of hprt mutation in B6C3F1 mice exposed to ozone alone and combined treatment of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone and/or dibutyl phthalate for 32 and 52 weeks" ppsx
... following exposure to ozone, NNK, and DBP, and combined treatments of NNK and DBP on ozone for 32- and 52-wk The procedures for isolating lymphocytes from spleen and culturing hprt mutant T-cell ... of mRNA from these clones For preparation of total RNA, frozen pellets of expanded clones were thawed on ice, and 1-10×103 cells were transferred into 500 µl Eppendorf tubes containing 10 µl RNase-free ... that additive and/ or synergistic responses occurred when both mice sexes were exposed to ozone, NNK, and DBP, and the combination of ozone, NNK, and DBP through chromosome aberration and supravital...
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Báo cáo y học: "Suppressive effect of secretory phospholipase A2 inhibitory peptide on interleukin-1β-induced matrix metalloproteinase production in rheumatoid synovial fibroblasts, and its antiarthritic activity in hTNFtg mice" ppt
... potently inhibits IL-1β-induced secretions of sPLA2 and matrix metalloproteinases (MMPs; 1, 2, 3, and 9) in RA synovial fibroblasts (SF), at protein and mRNA levels [11] Synovial fibroblast cell cultures ... Immunoassays and cell-based ELISA RA/OA SF samples were centrifuged briefly, and supernatants were stored at -20°C until used To assess the concentration of secreted proteins, supernatants of ... the standard curve (ng/ml sPLA2 on X-axis versus cpm/ml on Y-axis) and is expressed as ng/ml ± standard error of the mean Quantitative real-time RT-PCR After removal of supernatants for protein...
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Báo cáo y học: "Single amino acid change in gp41 region of HIV-1 alters bystander apoptosis and CD4 decline in humanized mice" potx
... experiments and wrote the paper AJ performed the experiments analyzed data and wrote the paper CY performed experiments PS and NM designed the study and wrote the paper All authors read and approved ... ligands J Virol 2007, 81:8180-8191 53 Mandl JN, Barry AP, Vanderford TH, Kozyr N, Chavan R, Klucking S, Barrat FJ, Coffman RL, Staprans SI, Feinberg MB: Divergent TLR7 and TLR9 signaling and ... TCID50 of viruses and bled every weeks for a total of weeks and then sacrificed (A) CD4 and CD8 levels in PBMCs from the mice were determined after staining with CD45, CD4 and CD8 antibodies...
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Báo cáo khoa học: " Systemic hypothermia increases PAI-1 expression and accelerates microvascular thrombus formation in endotoxemic mice" ppsx
... activation and temperature Statistics and graphics were performed using the software packages SigmaStat and SigmaPlot (Jandel Corporation, San Rafael, CA, USA) times of 255 ± 35 s and 238 ± 58 ... receptor GPIIb-IIIa, and, finally, platelet activation and clotting; impairment of the anti-thrombin, protein C and tissue factor pathway inhibitor anti-coagulative systems; and inhibition of fibrinolysis ... immunohistological sections and ELISAs, performed the statistics and drafted the manuscript BV conceived the study, participated in its design and coordination and helped to draft the manuscript MDM and EK participated...
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Báo cáo y học: "HIV DNA and Dementia in Treatment-Naïve HIV-1-Infected Individuals in Bangkok, Thailand"
... clades A, E, and B; and demonstrated equivalent amplification of the target gene Separation of PBMC Subsets and HIV DNA Analaysis Because HIV DNA is present in both lymphocytes and monocytes, ... Phatisawad, and Rapee Trichavaroj; Sean Hill, David Troelstrup, Erik Anderson, Andrew Williams, and Wichitra Apateerapong; and as well as acknowledge the SEARCH participants for their support and commitment ... 72°C/2 We used non-HIV-1 infected genomic DNA for a negative control and DNA from three HIV-1 infected cell lines (8E5, OM10.1, and ACH-2; NIH AIDS Research and Reference Reagent Program, NIH, Bethesda,...
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Phân tích, đánh giá chiến lược công ty TNHH 1 thành viên in trưởng thành công đến năm 2015 và khuyến nghị
... vực có liên quan đến an ninh trật tự biểu mẫu thống kê phục vụ mặt công tác nghiệp vụ lực lượng CAND,… Công ty có nhiệm vụ in ấn tài liệu tuyên truyền, thông báo tình hình an ninh trật tự hàng...
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Unit 1: A day in the life of. Listening
... WHILE-LISTENING Look at the pictures and describe them WHILE-LISTENING Pre order these pictures WHILE-LISTENING Listen and order these pictures WHILE-LISTENING Listen again and decide whether the statements ... to work T F POST-LISTENING V HOMEWORK - T asks ss to remember the story about Mr Lam and write about him and his daily routine ... stall - He drives passengers to everywhere they want HE IS A CYCLO DRIVER PRE-LISTENING Listen and repeat district:/'distrikt/ park:/pɑ:k/ routine:/ru:'ti:n/ pedal:/'pedl/ office:/'ɔfis/ food...
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An experimental investigation on hydrogen fuel injection in intake port and manifold with different EGR rates
... engine, International Journal of Hydrogen Energy, Volume 34, pp 9019-9032, 2009 [16] Addison Bain and Wm D Van vorst, The Hindenburg tragedy revisited: the fatal flaw found, International Journal of ... 2076-2895 (Print), ISSN 2076-2909 (Online) ©2010 International Energy & Environment Foundation All rights reserved International Journal of Energy and Environment (IJEE), Volume 1, Issue 2, 2010, ... 2076-2895 (Print), ISSN 2076-2909 (Online) ©2010 International Energy & Environment Foundation All rights reserved 224 International Journal of Energy and Environment (IJEE), Volume 1, Issue 2, 2010,...
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Unit 1-A day in the life of...
... the passage and find all the verbs that are used in past simple and the connectors in the story Simple past Regular verbs land stare announce Irregular verbs take begin S + Ved landed stared ... up a narrative about a hotel fire/ and / everybody / be / It/be/Saturday evening the discotheque / ground floor It / be / crowded /with people They / dance / and/ sing happily./ Suddenly / we ... up a narrative about a hotel fire/ and / everybody / be / It/be/Saturday evening the discotheque / ground floor It / be / crowded /with people They / dance / and/ sing happily./ Suddenly / we...
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The JSP Files (Part 1) - Purple Pigs in a Fruitbasket
... traffic increases and the load on the database and Web servers goes up The JSP architecture, on the other hand, involves more than one level, immediately making it more scalable and maintainable ... format float and long − used to store floating−point numbers boolean − used to store "true" and "false" values (note that unlike languages like C and PHP, JSP does not recognize => true and => false) ... " and " + beta + " is " + Sum); %> And the output is: The sum of 45 and 34 is 79 In this case, we've simply defined two variables as integer values, assigned values to them, and...
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Gián án Ụnit 1: A day in the life of...
... activities and time’s activities of Linh -2 ms for teams go to the board and write answers -team which more correct answer is winner * checking instruction (p.) *after ms T check answers and find ... BRAIN STORM -ask ss think about some their activities in a day in m -after m t call on some ss and ask them about their activities: T: Lan, what time you often get up? Lan:: I often get up at...
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Tài liệu Activity 5.1: Identifying Keys in the Logical Model pdf
... Identifying Keys In this exercise, you will identify primary, foreign, and composite keys for a logical data model based on the Ferguson and Bardell, Inc case study ! Specify the keys in a logical data ... Key: (FK) Composite Key: (CK) Draw a line under the attributes used to define the composite key, and then label their types as Primary or Foreign Next, you will discuss your answers with the class...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25