detection of mycobacterium tuberculosis complex in respiratory specimens by using the bd probetecet direct detection assay
Báo cáo khoa học: "Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India" docx
... esehT tnalugaocitna gniniatnoc ton sebut muucav ni derots dna niev raluguj eht morf )lm 01( nekat erew selpmas doolb noitazilibommi nim rof noitnetnoc knurt a ni lamina hcae gniniartser dna ,yad ... ,yad gnilpmas eht fo gninrom eht ni larroc a ot slamina gnidael yb demrofrep saw hcihw ,noitazilibommi retfa ht06 dna ,ht54 ,ht83 ,ht13 syad no nekat erew selpmas doolB )slamina fo rebmun eht yb ... lamina aigolotamilcoib erbos sartselap ed lanoicanretni olciC º1 )de( PRJM atsoC :nI lamina oãçudorp e essertsE OR oãçanracnE 11 0002 ,kroY weN ,gnihsilbuP IBAC ,011-77.pp erafleW laminA rof...
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Báo cáo y học: " Profile of subjective quality of life and its correlates in a nation-wide sample of high school students in an Arab setting using the WHOQOL-Bref" pot
... satisfied with their circumstances of living, - and their average QOL domain scores would be high, in comparison with the international data - In view of the robust findings in the literature, ... for the cooperation of the school’s psychologists At the preliminary stage of the study, the research team hosted the psychologists of the selected schools at meetings facilitated by the Ministry ... study schedule In the few days between explaining the nature of the study and the completion of the questionnaires, the students were requested to inform their parents about the study, in case any...
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Guidelines for Preventing the Transmission of Mycobacterium tuberculosis in Health-Care Settings, 2005 pot
... treatment of TB disease Training should also include the role of the HCW in educating patients regarding the importance of reporting symptoms or signs of TB disease and the importance of reporting ... absence of infection with M tuberculosis In the context of TST screening as part of a TB infectioncontrol program, the interpretation of TST results occurs in two distinct parts The first is the interpretation ... screening is performed in the setting, review the results of screening of other HCWs in the same area of the health-care setting or same occupational group If serial TB screening is not performed in...
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Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx
... Their reaction formally consists of the activation of the amino acid by adenylation (Eqn 1) followed by transfer of the amino acyl residue to the 2Â-OH or 3Â-OH position of the 3Â-OH end of the ... except that the concentration of one of the substrates was varied and the levels of the others xed In these assays the amount of enzyme was chosen in order to observe linearity up to 10 min, and ... [2,4] In these aaRS the binding of tRNA induces an ATP productive binding mode [5] GluRS are also distinguished on the basis of their ability to discriminate between tRNAGlu and tRNAGln The discriminating...
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Báo cáo khoa học: Molecular dissection of the biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and permeability of Mycobacterium tuberculosis doc
... that of DIM B, due to the reduction of the keto function of DIM B, followed by the methylation of the resulting hydroxyl group Thus, inactivation of the Rv2951c gene in M tuberculosis abolishes the ... determined by calculating the ratio of mutant to wild-type bacteria after correcting for the ratio of these strains in the inoculum It appeared that, 21 days after infection, the ratio of PMM56 ... containing Km, no colonies were obtained for four mice, indicating that fewer than 50 bacteria of the PMM56 mutant strain were present in the spleen of these mice For the determination of the...
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Báo cáo khoa học: "Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR" pot
... present in the porcine intestine and feces including swine genomic DNA The number of amplification cycles was one of the important factors for increasing the sensitivity of the PCR assay The increase ... methods include H E staining and silver staining of the intestinal sections 36 Dong-Kyun Suh et al Fig Section of the ileum from a pig infected with L intracellularis a : Presence of numerous intracellular, ... determine the accuracy of the PCR assay for the detection of L intracellularis infection (Table 1) Mucosal scraping and fecal samples were obtained from each pig Of 17 mucosal scrapings, 16 specimens...
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Báo cáo y học: "Glutathione and growth inhibition of Mycobacterium tuberculosis in healthy and HIV infected subjects" pot
... describing direct and indirect effects of GSH in growth control of H37Rv in blood cultures derived from healthy and Figure HIV-infected subjects Model describing direct and indirect effects of GSH in ... In other words, secretion of IL-6 by M tuberculosis- infected macrophages may contribute to the inability of IFN-γ to eradicate M tuberculosis infection [26] The high levels of IL-6 released by ... terminated at h & 48 h after infection, to determine the intracellular viability of H37Rv Intracellular viability of H37Rv was determined by plating the diluted blood cell lysates on 7H11 Data in...
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Understanding cofactor f420 dependent mechanism(s) in the activation of bicycle nitroimidazoles and in the physiology of mycobacterium tuberculosis
... substrates by investigating reoxidation of F420H2, production of NO and by determining binding constants of the analogs to the protein A direct chemiluminescence-based NO detection assay to measure the ... production, inhibition of Mtb FAS I (Ngo et al., 2007;Zimhony et al., 2007) or the inhibition of transtranslation by the binding of pyrazinoic acid to the ribosomal protein S1 (RpsA), thereby explaining ... the physiologic significance of their activating machinery in Mtb form the basis for the research work described in this thesis Before detailing the objectives of the thesis in section 1.6, the...
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Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf
... WhiB2, the amino acid immediately after the C-terminal cysteine of the CXXC motif is proline (Fig S6) In WhiB2 the absence of proline and therefore of the kink might not allow the protein to attain ... of cysteines The involvement of four conserved cysteines in the coordination of the [4Fe–4S] cluster has been confirmed in WhiB3 [9] and WhiB4 [15] The brown color of the purified protein and the ... resistance to intracellular killing, the scavenging of free radicals and the reactivation of degenerated proteins during infection by a family of proteins named ‘thioredoxins’ is one of the elegant...
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A genome wide association study of pulmonary tuberculosis susceptibility in Indonesians doc
... search across the genome, and suggests multiple targets working in novel pathways involved in the host containment of infection with TB, further providing insights on the mechanism of this disease, ... one another [22] The result of the lambda inflation factor calculated according to the method of Devlin and Roeder [19], had a value close to 1, which further confirmed that there was minimal ... is the primary site of infection by M tuberculosis where, responding to invasion, our body reacts by recruiting immune cells and proinflammatory cytokines to attack and control further dissemination...
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Management of pulmonary tuberculosis patients in an urban setting in Zambia: a patient’s perspective ppt
... volunteers in the implementation of DOTS has contributed to the improvement in cure rates over the past decade from 67% in 2000 to the global target of 85% by 2006 [3] The goal of the Zambian ... were also obtained from the Director of the NDHMT Consenting respondents were asked to sign an informed consent following an explanation of the study Interviewers were not part of the health care ... afford the time at the health centre Further, the period of the study, were the early days of scaling up of free antiretroviral therapy in Zambia and so people may still have been feeling helpless...
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Báo cáo khoa học: The sigma factors of Mycobacterium tuberculosis: regulation of the regulators potx
... point mutation in the 4.2 domain of rA (R515H) resulted in attenuation of virulence of the M bovis strain ATCC 35721 in a guinea pig model of infection [27] The mutated rA was reported to be incapable ... number of such results have been reported in the last few years, establishing the role of rA in host– pathogen interactions, in addition to its housekeeping function In a clinical strain (the M tuberculosis ... factor-a-a, interleukin-1b, interleukin-6 and interferon-c in the lungs of the infected animal [51] The attenuation of the sigC mutant may result from dysregulation of expression of several key...
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Báo cáo Y học: Trehalose-phosphate synthase of Mycobacterium tuberculosis Cloning, expression and properties of the recombinant enzyme pdf
... contained various amounts of the recombinant TPS After an incubation of 15 min, one set of incubation mixtures was assayed by the anthrone colorimetric method and the other set was assayed by the ... compared to the amount of NAD+ produced (nmol) in the coupled assay, as an indirect measure of the amount of trehalose-P synthesized However, for most of the studies described here, the anthrone assay ... TPS The effect of substrate concentration on the activity of the TPS was determined as shown in Figs and Fig shows the effect of increasing concentrations of either UDP– glucose or GDP–glucose in...
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Báo cáo khoa học: Characterization of Mycobacterium tuberculosis nicotinamidase/pyrazinamidase ppt
... residues (Fig 3B) On the basis of these criteria, nine residues were chosen for further study (Table 1), including the The presence of metal ions in PncA was determined using inductively coupled ... summarized in Fig The maximum restoration of activity was attained using approximately 200 lm of metal ion In the presence of Fe2+, however, the restoration of enzyme activity when using PZA as ... place by the Asp52, His54 and His71 residues, and the corresponding His58 (His57 in M tuberculosis) residue is not involved in metal ion binding [23] Furthermore, the enzymatic activity of M tuberculosis...
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Understanding Tuberculosis – Analyzing the Origin of Mycobacterium Tuberculosis Pathogenicity Edited by Pere-Joan Cardona potx
... process The colour of the arrows shows the ability to induce a process (in gray) or inhibit it (in red), and the thickness of the arrow is proportional to the intensity of this induction The upper ... Understanding Tuberculosis – Analyzing the Origin of Mycobacterium Tuberculosis Pathogenicity One growing issue is trying to lie Mtb by favoring artificially their growth stopping for instance the inflammatory ... differentiate into epithelioid cells The neutrophil influx increases and the centrum of the granuloma necrotizes and then caseates resulting in the formation of necrotizing and caseating granulomas In necrotizing...
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Báo cáo sinh học: " Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" potx
... by HSV probe specific binding assay HSV type was confirmed in samples in which an equivocal typing result was initially obtained HSV type was confirmed by repeating the realtime assay using the ... by these changes (Figure 1) Of the samples confirmed by Rotorgene, of these had no mutations in the probe binding area The remaining samples resulted in poor quality sequencing reactions and the ... effectiveness of any current intervention, determining the efficacy of drugs, assessing drug resistance and are useful research tools in the study of the epidemiology of transmission The discrimination of...
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báo cáo hóa học:" Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" pdf
... by HSV probe specific binding assay HSV type was confirmed in samples in which an equivocal typing result was initially obtained HSV type was confirmed by repeating the realtime assay using the ... by these changes (Figure 1) Of the samples confirmed by Rotorgene, of these had no mutations in the probe binding area The remaining samples resulted in poor quality sequencing reactions and the ... effectiveness of any current intervention, determining the efficacy of drugs, assessing drug resistance and are useful research tools in the study of the epidemiology of transmission The discrimination of...
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Báo cáo hóa học: " Research Article Genome-Wide Analysis of Intergenic Regions of Mycobacterium tuberculosis H37Rv Using Affymetrix GeneChips" docx
... understanding about promoter and cis-regulatory elements since the former is involved in binding of RNA polymerase and the latter in binding transcriptional factors but the DNA-protein binding process ... reported in the M tuberculosis H37Rv genome (Table 2) A hypothetical protein L M Fu and T M Shinnick Table 3: The locations of new hypothetical proteins found in the genome of Mycobacterium tuberculosis ... results in bioinformatics and genomics rather than the inconsistency in stringency of computational parameters used The integrity and advancement of the knowledge base in genomics would hinge upon the...
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UNDERSTANDING TUBERCULOSIS – ANALYZING THE ORIGIN OF MYCOBACTERIUM TUBERCULOSIS PATHOGENICITY docx
... process The colour of the arrows shows the ability to induce a process (in gray) or inhibit it (in red), and the thickness of the arrow is proportional to the intensity of this induction The upper ... Understanding Tuberculosis – Analyzing the Origin of Mycobacterium Tuberculosis Pathogenicity One growing issue is trying to lie Mtb by favoring artificially their growth stopping for instance the inflammatory ... differentiate into epithelioid cells The neutrophil influx increases and the centrum of the granuloma necrotizes and then caseates resulting in the formation of necrotizing and caseating granulomas In necrotizing...
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Báo cáo y học: "Early Detection of cytokine protein expression in mouse lung homogenates using suspension bead array" ppt
... protein expression in lung tissue homogenates using suspension bead array These findings correlated with cytokine protein expression profiles detected using antibody array and were confirmed using ... further elucidate diagnostic markers indicative of the LPS-evoked lung inflammatory injury response While, traditional immunohistochemical staining methods for detection of cytokine protein expression ... LPS-treated (n = 4) groups using the RayBio Mouse Cytokine Antibody Array kit according to the user manual The signals were detected using the ECL kit according to the user manual, exposed on...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng 9 tr 25