design of hammerhead ribozymes and allosterically controllable maxizymes for cancer gene therapy
Báo cáo Y học: A novel gain-of-function mutation of the integrin a2 VWFA domain docx
... have generated a mutant form of the a2 VWFA domain which shows a gain -of- function, and which may result in the promotion of the open form Helix a7 is therefore seen to be a valid target for such ... Kunkel [39] and the BIO-RAD Muta -Gene mutagenesis kit Essentially, single-stranded uracil-containing DNA was generated by helper phage infection of Escherichia coli strain CJ236 and then used ... identical for a2-E318W (s) and wild-type (d), and is shown for comparison Microtitre plates were coated with lgÆmL)1 VWFA domain and the binding of antibodies measured over a range of concentrations...
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Some aspects of American culture and society in the twentieth and twenty-first centuries through a number of selected short literary works
... writers for a worthy piece of literary work often come from real life Therefore, it can be understood that literature is a tool for the reflection of life and for the expression of viewpoints of the ... all-colored world of Amos and Andy” which is full of black lawyers, black doctors and nurses “We were starved for images of ourselves and searched TV to find them.” (p.1089) But for other fields, ... beautiful and lively with variety of colors such as purple, yellow, pink of the walls; dark green, white and yellow of the notocactus and cactus, and all kinds of fragrance from the flowers and herbs...
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Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx
... The activation of CD4+ cells, however, dramatically induces transcription of the ITK gene and is key for the productive infection of HIV in these cells Accordingly, the inhibition of ITK activity ... [67] Moreover, as a result of chromosomal translocation, a chimera, consisting of the dimerizing TEL protein and SYK, was formed and has been shown to cause a rare form of myelodysplastic syndrome ... family of cytoplasmic enzymes that are important for several aspects of leukocyte biology Both loss- and gain -of- function mutations in humans have been instrumental in our understanding of their...
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báo cáo khoa học: " pax1-1 partially suppresses gain-of-function mutations in Arabidopsis AXR3/IAA17" pdf
... type and for each backcross, the F2 generation was analysed for segregation of the mutant phenotypes 55 and 94 F2 plants were analysed from the first and second backcrosses, respectively, and ... analysis of AXR3::GUS expression, contributed toward the mapping and design of the study, and drafted the manuscript JJ performed the analysis of axr3 protein turnover, ARF19 allelism tests, and contributed ... plants and amplified with primers for SSLP, CAPS and SNP markers listed at The Arabidopsis Information Resource (TAIR) [60] Primer sequences and restriction sites for markers nga392, M59 and CAT3...
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Báo cáo khoa học: The transcription factor ZBP-89 suppresses p16 expression through a histone modification mechanism to affect cell senescence doc
... against Flag and GFP (Upstate; 06-896) with gentle shacking for h at °C, and this was followed by the addition of 40 lL of protein A–agarose for another h The beads were resuspended in 100 lL of 2· ... appears to inhibit gene activity by binding to DNA independently of Sp1 [7] Reversible acetylation of internal lysine residues of the N-terminal domains of nucleosomal histones and the resultant ... important epigenetic mechanisms in the regulation of gene transcription The interplay between histone acetyltransferases (HATs) and HDACs is critical to the dynamics of chromatin structure and function,...
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Báo cáo hóa học: " Existence results for a class of nonlocal problems involving p-Laplacian" pot
... real Banach space and X2 ≠ {0}, and is finite dimensional Suppose J Î C1(X, R) satisfies (PS) and (i) there is a constant a and a bounded neighborhood D of in X2 such that J|∂D ≤ a and, (ii) there ... http://www.boundaryvalueproblems.com/content/2011/1/32 Page of hypotheses (F0), (F1), (F2) and (1.2) are clearly satisfied f (x, u) = arctan u + u , + u2 hypotheses (F3), (F4) and (F5) and (1.2) are clearly satisfied Proofs of the theorems ... u)dx Proof of Theorem 2.1 By (F0), we know that f(x, 0) = 0, and hence u(x) = is a solution of (1.1) To complete the proof we prove the following lemmas Lemma 3.1 Any bounded (PS) sequence of J...
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CHARACTERIZATION OF INHIBITORS OF ALDEHYDE DEHYDROGENASE 2 IDENTIFIED THROUGH A HIGH-THROUGHPUT DOCKING APPROACH
... I74 and I78 on the dehydrogenase activity of ALDH2 Fig IC50 curves for Inhibitors I32, I72, I74, I76 and I78 on the esterase activity for ALDH2 Fig Competition for the NAD-catalyzed activation of ... peroxidation, and, thus, help maintain cellular homeostasis Increased expression and activity of ALDH isozymes are found in many human cancers and are often associated with poor prognosis (95, 96) Therefore, ... ALDH2 and ALDH3 isozymes in the function of conserved Glu333 and Glu209 residues and the differential effect of NAD+ binding on the esterase reaction (29) Exploitation of these functional and structural...
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Báo cáo y học: "Gain of a 500-fold sensitivity on an intravital MR Contrast Agent based on an endohedral Gadolinium-Cluster-Fullerene-Conjugate: A new chance in cancer diagnostics"
... agent.[60-62] Therefore a progress in the precision of therapy like the intensity-modulated radiation therapy (IMRT) and the use of heavy ions demands absolute reliability of new diagnostics and treatment ... Peter Lorenz and Heinz Fleischhacker (DKFZ) for technical assistance and for critical reading of the manuscript Conflict of Interest 17 18 19 The authors have declared that no conflict of interest ... appended to the C-termini of the NLS and the CPP peptides for disulfide bond formation between these modules This enables the intracellular enzymatic cleavage and dissociation of the CPP from the NLS...
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Tài liệu Báo cáo khoa học: C-Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism doc
... an isoform of ErvC which is present in the latex of the plant Materials and methods vector [16] A fragment of the original cDNA encoding the prodomain, the mature domain and CT-extension of Erv-C ... vector portion and ‘aa’ stands for amino acids (IV) The amino acid sequence of the open reading frame The sequence of CT-extension is in red (B) Lanes and 2, Purified proteins rproErvC+CT and rproErv-CDCT, ... activity of the enzyme against a small peptide Thermal stability Temperatures of maximum proteolytic activity (Tmax) for rproErv-C+CT and rproErv-CDCT are 50 and 45 °C (Table and Fig 4) and they...
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Tài liệu Controlling Fine Particulate Matter Under the Clean Air Act: A Menu of Options pdf
... meeting the current PM2.5 standards would avoid tens of thousands of premature deaths annually and save hundreds of thousands of people from significant respiratory and cardiovascular disease The ... for various types of sources and air pollutants between the states and localities on the one hand, and the federal government on the other Generally, the states and EPA share responsibility for ... standards for PM, SO2 and NOx for industrial and commercial boilers These disparities suggest that there is significant room for improvement in the emissions profile of this source category For...
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Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx
... induction Effects of a-T and its derivatives on the enhancement by TS of LPS/IFN-induced NO production We compared the effects of a-T and its derivatives TA and TN with that of TS on LPS/IFN-induced ... production with Ro31-2880 and GF109203X were approximately 70 and 30%, respectively Effects of TS on the amount of PKCa We further examined the effects of TS on the amounts of PKCa and PKCb by Western ... upregulation of PKCa DISCUSSION Fig Effects of PKA inhibitors (KT-5720 and H8) and PKC inhibitors (Ro31-8220 and GF109203X) on the enhancement by TS of LPS/IFNinduced NO production in VSMC (A), and Western...
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Tài liệu Playing through: A Guide to the Unwritten Rules of Golf potx
... Intricacies of Golf Attire 25 Respect the Course 36 Carts and Greens and Traps and Water 47 Where to Stand: The Theory of the “X” 57 The Biggest Frustration by Far: Slow Play 63 “Fore!” Doesn’t ... Collins for all her counsel and editing of the manuscript To Andy Pazder at the PGA Tour, who helped me understand the intricacies of spectator etiquette To the people at The Emily Post Institute for ... every day! I’ve seen our former golf professional work hours with our junior golfers at no charge He was a fantastic promoter of junior golf and of the game of golf in general Bottom line: The...
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Đề tài " Approximating a bandlimited function using very coarsely quantized data: A family of stable sigma-delta modulators of arbitrary order " pptx
... books [2] and [15] This literature is mostly concerned with the design of, and the study of good design criteria for, more complicated Σ∆-schemes The one given by (8) is the oldest and simplest ... argument formally, for schemes of arbitrary order; the proof consists essentially of careful repeats of the last paragraph at every level This then also leads to estimates for the bounds Mj , and ... ≤ a for all n, and (u0 , v0 ) ∈ Aa , then (un , ) ∈ Aa for all n ∈ N; see [19] APPROXIMATING A BANDLIMITED FUNCTION 693 It follows that we have uniform boundedness for the un if xn = f n for...
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THE EFFECTS OF MEGAMERGERS ON EFFICIENCY AND PRICES: EVIDENCE FROM A BANK PROFIT FUNCTION docx
... Humphrey, and pulley (1995), Berger, Cummins, and Weiss (1995)> PulleY a n d Humphrey (1995) for previous specifications of the nonstandard form of revenue and profit functions Note that the nonstandard ... Appendix standard profit efficiency model in equations (1) and (2) above, and Table A2 gives the estimated parameters of the model The level of profit efficiency the ratio of predicted profits to ... Columns and of Table show the same pre- and post-merger efficiency and profitability ranks and changes in rank for merging banks with in lowest and highest thirds, respectively, in terms of premerger...
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Báo cáo khoa học: Emergence of a subfamily of xylanase inhibitors within glycoside hydrolase family 18 pdf
... rXIP-I consisted of three molecular isoforms of pI 6.8–7.2–8.2 with a main band at pI 7.2 This value differs from native XIP-I of pI 8.7–8.9 [6], due to the insertion of the myc epitope and His-tag ... chitinase) for pRIXI and rXIP-I, respectively The specificity of pRIXI towards fungal and bacterial GH10 and GH11 xylanases was compared to that of rXIP-I (Table 1) The pattern of inhibition of rXIPI ... addition of the enzymes: A niger xylanase (53 pmol), XYNC (30 pmol) and T longibrachiatum M3 xylanase (40 pmol) and carried out at 30 °C for 10 for A niger xylanase or for for XYNC and T longibrachiatum...
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Báo cáo khoa học: The oxidative effect of bacterial lipopolysaccharide on native and cross-linked human hemoglobin as a function of the structure of the lipopolysaccharide A comparison of the effects of smooth and rough lipopolysaccharide ppt
... presence and absence of EDTA (Fig 1) The rate of oxidation was increased in the presence of the LPSs of both the smooth and rough E coli, but the rate of oxidation in the presence of the LPS of smooth ... fast phase of the reaction, but decreased the rate of the slow phase of oxidation in the presence of EDTA A comparison of rough and smooth LPSs of E coli and S minnesota in the presence of EDTA ... Hb, the rates of oxidation were identical for the LPSs of smooth and rough S minnesota, both in the presence and absence of EDTA For both the smooth and rough LPSs, the effect of EDTA was to...
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Báo cáo khoa học: Macrocypins, a family of cysteine protease inhibitors from the basidiomycete Macrolepiota procera pot
... Doc S1 and Doc S2 and Fig S1 and S2) Cloning and analysis of macrocypin cDNA and gene sequences The N-terminal sequence (H2N-GLEDGLYTIRHLVE GQPPNIPGGMYASSKDGKDXPVTAEPPLP) and the sequence of an ... contents of proline, of around 9.2% (the average overall protein content is 4.7 %), of tryptophan, of 3.6% (average 1.1 %) and of tyrosine, of 6.0–6.6% (average 2.9%), and low leucine contents of around ... (Table S1) Forward and reverse degenerate primers (forward 1N-mar-Clit, nested forward 2N-mar-Clit and reverse C-mar-Clit) were designed based on the N-terminal amino acid sequence and an internal...
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Báo cáo khoa học: Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ pdf
... eluted (14–22) with 360 lL of 4% (w/v) SDS (for analysis) or 0.1 M glycine/HCl, pH 2.3 (for purification) The amount of polypeptide in each 40 lL fraction and the ratio of the antigen-binding fraction ... (Fig 1Cb), 65% in (Fig 1Cc), and 85% in (Fig 1Cd), respectively Therefore, the DTT-deficient condition in the presence of PDI was effective for the disulfide formation and increased the soluble polypeptides ... describe here suitable conditions for the cell-free synthesis of the active disulfide-containing scFv Materials and methods Materials The gene encoding the scFv fragment of the Se155–4 IgG antibody,...
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synthesis and growth of hematite nanodiscs through a facile hydrothermal approach
... probably play an important role of in the formation of such nanostructures However, the nature is still not clear Therefore, further study needs to be performed for understanding the hollow nanostructures ... absence of surfactants under mild conditions, and no need of toxic precursors and high-temperature decomposition in organic solvents The nanodiscs (diameters of *50 nm and thickness of *6.5 nm) ... thickness of *6.5 nm Combining the structural analysis of TEM and AFM, the as-prepared hematite particles are of nanodiscs in 123 J Nanopart Res Particle nucleation and growth Fig a TEM image of hematite...
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