culturing and differentiating human mesenchymal stem cells for biocompatible scaffolds in regenerative medicine
Báo cáo hóa học: "Early combined treatment with sildenafil and adipose-derived mesenchymal stem cells preserves heart function in rat dilated cardiomyopathy" doc
... embryonic stems cells and bone marrow-derived mesenchymal stem cells, adiposederived mesenchymal stem cells (ADMSCs) have the distinct advantages of being abundant, easy to obtain with minimal invasiveness, ... lower in group than in groups 3, 4, and 5, significantly lower in group than in groups and 5, and also notably lower in group than in Page 10 of 16 group This finding indicates that early combined ... stained cells in LV myocardium were significantly higher in group than in other groups, significantly higher in groups and than in groups and 5, and also notably higher in group than in group Lin...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... spun at × 103 rpm for five minutes, resuspended in complete DMEM (containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin), and plated at to × 107 nucleated cells per 75 cm2 flask ... double labelling staining solution for 10 minutes at room temperature After staining, aggregates were washed five times with 100 μL of binding buffer, fixed overnight in PBS-buffered 4% paraformaldehyde, ... differentiation and apoptosis We used staining for ALP and immunohistochemistry for COL X as markers for chondrocyte hypertrophy (Figure 4) No detectable ALP and only weak COL X immunostaining was seen in...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... expression of insulin and proinsulin in stage IV cells Quantification of IHC staining shows FN and LAM increases the percentage of (B) proinsulin, and (C) insulin positive cells (mean ± S.D.; ** indicates ... differentiation with increases in insulin and Glut2 gene expressions, proinsulin and insulin protein levels, accumulation of cytoplasmic granules including a, b, and δ granules, and insulin release in response ... doi:10.1186/1423-0127-17-56 Cite this article as: Lin et al.: Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK Journal of Biomedical...
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Báo cáo y học: "Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells" pot
... I, III and X, the catabolic enzymes matrix metalloproteinases (MMPs) and A distintegrin and metalloproteinase with thrombospondin motifs (ADAMTSs) and inflammatory cytokines interleukin-1b (IL-1 ... Excitation and emission for Calcein and ETH-2 were 494/517 nm and 528/617 nm respectively with Calcein staining the cytoplasm of live cells green and ETH-2 staining the nuclear envelope of dead cells ... the intact pellet, pellets were first fixed in formalin and then incubated with 1% Alcian Blue in HCl for 30 minutes, followed by final fixation in 100% ethanol Pellets were embedded in freezing...
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Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
... nanofibrous scaffolds on day 14 Dual labeling includes tetramethylrhodamine isothiocyanate (TRITC)-conjugated phalloidin (staining red F-actin) and DAPI (staining blue nuclei) The staining showed the formation ... Chan and Seeram Ramakrishna Stem Cell Response to Biomaterial Topography In: Murugan Ramalingam, Seeram Ramakrishna and Serena Best, editors Biomaterials and Stem Cells in Regenerative Medicine ... Chan, and Seeram Ramakrishna Mineralized electrospun nanofibrous scaffolds for directing osteogenic differentiation of human mesenchymal stem cells Proceedings of the 241st ACS Meeting and Exposition,...
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Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1
... derived human fetal mesenchymal stem cells through paracrine signalling mechanisms in vitro and induces neovasculogenesis in vivo prior to bone repair Tissue Engineering and Regenerative Medicine International ... importance of continuous hypoxic exposure for the culture of human fetal mesenchymal stem cells in bone tissue engineering applications 3rd Tissue Engineering and Regenerative Medicine International ... Osteogenic Cells 46 2.8.2.2 Mesenchymal Stem Cells 48 2.8.2.3 Mesenchymal Stem Cells in Bone Tissue Engineering .49 2.8.2.4 Proposed Cell Type – Human Fetal Mesenchymal Stem Cells...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving ... necessary for the osteogenic differentiation of human mesenchymal stem cells on laminin-5 J Cell Biochem (2):499-514 102 Lozito TP, Kuo CK, Taboas JM, et al 2009 ;Human mesenchymal stem cells express...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... induced to form EBs in serum-supplemented medium for days prior to dissociation into single cells and co -culturing with human nasal chondrocytes in a transwell insert system for up to weeks in ... from adult stem cells and mature chondrocytes These include culturing in defined medium to which chondro-inductive cytokines, growth factors and chemicals have been added, co -culturing and the addition ... with lacunae These findings indicate the importance and relevance of biomaterials in cartilage tissue engineering and regenerative medicine An ideal scaffold for engineering a cartilage implant...
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Characterization of adult human bone marrow mesenchymal stem cells for effective myocardial repair
... Likewise, M-band proteins such as myomesin, M protein and the C-terminus of titin play a significant role in incorporating the myosin thick filaments into regular A bands19-25 Importantly, the coincident ... bodies composed of -actinin, the N-terminus of titin, nebulin, and telethonin contribute to the polarized organization of the thin actin filaments to form I-Z-I bands in developing sarcomeres11, 13-18 ... Scientific Meeting incorporating 14th SGH-Stanford Annual Joint Update and Annual Evidence-Based Medicine Seminar, 27-28 April 2007, Singapore v Genevieve MY Tan, Ling Qian, Ying Ying Chung, Yacui...
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Báo cáo y học: "An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue" pot
... and 3,3-diaminobenzidine disclosure) for aggrecan and the staining intensity increased with time post injection (Figures and 5b) Aggrecan matrix staining within the vicinity of the injected cells ... following injection, and the intensity of the matrix staining increased with time in culture (Figures and 5b) Weak staining for type II collagen was observed within a small number of cells in monolayer ... viability/metabolic activity of injected mesenchymal stem cells Photomicrographs representative of caspase immunopositivity and polyAcells mRNA staining in mesenchymal stem cells injected into tissue explants...
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Báo cáo y học: "Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells" ppsx
... replacement for degenerative OA or RA Synovial tissues were finely minced and digested with 0.2% collagenase in DMEM containing 10% fetal bovine serum, 100 U/ml penicillin and 100 µg/ml streptomycin (complete ... characterization For flow cytometry, cells were harvested by treatment with 0.05% trypsin and 0.53 mM ethylenediamine tetracetic acid, and were resuspended in PBS containing 0.1% BSA and Available online ... shown) Inversely, the mean induction of osteocalcin and alkaline phosphatase was statistically higher in cells from BM than in cells from the synovium In these conditions, the primary skin fibroblastic...
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Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
... healing in animal models [13-15] Recombinant human BMP-2 and BMP-7 are used clinically in spinal fusion and the healing of tibial fractures To obtain a clinically acceptable result, these proteins ... of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells ... small interfering RNA (siRNA) on chordin expression Mesenchymal stem cells (MSCs) were transfected with either control or chordin siRNA and then grown in OM for the or 10 days RNA was extracted and...
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Transplantation of mesenchymal stem cells for the treatment of parkinsons disease in a mouse model
... various methods for inducing MSCs into neuron-like cells have been reported in vitro and the systemic injection of MSCs in mice with brain injury had positive effects on injury repair, incompatible ... is therefore conceivable that in PD, as in humans, primates and rodents exposed to MPTP, an acute insult initiates an inflammatory reaction that becomes self sustaining after the initiating agent ... protein involved in the degradation of cytoplasmic proteins The function of α-synuclein is still not clear In the healthy brain, α-synuclein is present in the presynaptic terminals in the SN and...
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Human embryonic stem cells for genotoxicity testing
... Figure hESCs maintained at oC for 24h by PNA FISH hESCs maintained at 25 oC for 24h by PNA FISH 33 34 Figure hESCs maintained at oC for 48h by PNA FISH 34 Figure hESCs maintained at 25 oC for 48h by ... spread of hESCs maintained at oC for 48h by mFISH 38 Figure 14 Metaphase spread of hESCs maintained at oC for 48h by mFISH 39 Figure 15 Karyotype of hESCs maintained at oC for 48h by mFISH 39 ... by MTT assay following exposure of hESC to reduced temperature (4oC and 25oC) for 24h and 48h Table Percentage of aberrant cells in untreated controls and in MMCtreated IMR90 and hESCs by PNA-FISH...
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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt
... washes and incubation with the nuclear stain 4¢,6-diamidino-2-phenylindone for 20 min, the MSCs were washed in NaCl ⁄ Pi for 10 and mounted in gelvatol for microscopic imaging Protein extraction and ... plated in IMDM supplemented with 15% heat-inactivated fetal bovine serum and 100 UÆmL)1 penicillin ⁄ streptomycin and incubated at 37 °C in a humidified tissue culture incubator containing 5% ... The cells were then fixed in 2% paraformaldehyde in NaCl ⁄ Pi for 30 min, washed twice with NaCl ⁄ Pi and permeabilized with 0.3% Triton X-100 in NaCl ⁄ Pi for 10 Next, the MSCs were blocked in...
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Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc
... factor in determining stem cell transdifferentiation Discussion AT-MSCs may be good candidates as stem cells for cell transplantation and tissue engineering in regenerative medicine, as a large ... apolipoprotein A-I apolipoprotein A-II apolipoprotein B apolipoprotein C-I apolipoprotein C-II apolipoprotein D apolipoprotein E apolipoprotein F apolipoprotein H fatty acid binding protein 1, liver ... 43 °C Finally, the chip was rinsed in 0.05· SSC before drying by low-speed centrifugation For staining, the chip was immersed in an NaCl ⁄ Pi solution containing 10 lgÆmL)1 of streptavidin ⁄ R-phycoerythrin...
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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt
... ,etahpsohp lorecylg -β fo detsisnoc taht muidem noitcudni cinegoetso na desu ew ,sCSM eht morf noitaitnereffid cinegoetso ecudni oT laitnetop cinegoetso tibihxe sCSM doolb droC )A2 giF( nitnemiv dna ... ecnanetniam eht dna euqinhcet reporp eht ,erutluc yramirp ot gnilpmas morf emit eht taht thguoht saw tI ]72,22[ srotinegorp citeiopotameah rof esac eht sa emas eht ,srotinegorp lamyhcnesem ni ... ladiobuc wohs ton did sCSM BCU eht ,sCSM worram enob ekilnu ,revewoH )4 giF( )gniniats assoK-noV yb deifitnedi( laitnetop cinegoetso evah sCSM taht gnitseggus ,etitapayxordyh demrof sCSM BCU ehT enosahtemaxed...
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Báo cáo y học: "Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix" ppsx
... saline for 20 minutes and stored in 70% ethanol until processed for paraffin embedding Proteoglycan production in the ECM was visualised by toluidine blue staining (Sigma, St Louis, MO; 0.1% in ... Eberhardt M, Linscheid P, Christ-Crain M, Keller U, Muller B, Zulewski H: Human adipose tissue-derived mesenchymal stem cells differentiate into insulin, somatostatin, and glucagon expressing cells Biochem ... and assayed for sulphated proteoglycan production using the DMB assay [26] Scoring of immunohistochemistry and toluidine blue staining Scoring of slides from immunohistochemical staining of cellsurface...
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Báo cáo y học: "Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions" pdf
... Immunostaining of anti-a-actinin and anti-a MHC in cells at different time points of culturing A strong expression of both a-actinin and MHC proteins (A, B, D, E, G, H, J, K) was observed in CMCM and ... both a-actinin and MHC proteins, but not those cells from DMEM+S1P group Figure shows the time dependent expression and the percentage of cells expressing a-actinin and sarcomeric a/b myosin cardiac ... by spin coating using a single wafer spin processor (Laurell Technologies, North Wales) at 3000 rpm and the spin coating time of 20 s The coated films were dried in air for at least 30 and then...
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Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx
... Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Pittenger MF, Mosca JD, McIntosh KR: Human mesenchymal stem cells: progenitor cells for cartilage, bone, fat and ... tissue engineering by providing abundant seeding cells with high quality List of abbreviations ADSCs: adipose-derived mesenchymal stem cells; MSCs: mesenchymal stem cells; ALP: alkaline phosphatase; ... capacity in vitro for up to 12 passages Since no unique molecular marker for mesenchymal stem cells has been established we used multiple surface markers for hADSCs identification Mesenchymal stem cells...
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