construction purification and characterization of escherichia coli rna polymerases tagged with different fluorescent prote
Báo cáo hóa học: " Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus" pot
... the Protein and Proteomics Centre (NUS, Singapore) Tandem MS analyses for these peptides were carried out at the Laboratory of Protein and Proteomics Centre (NUS, Singapore) using a MALDI-TOF-TOF ... the production of aquatic animal feeds and compost In our study, an extensive purification of these proteases was carried out together with the characterization of temperature and pH optimum, ... chromatography of AEX and HIC was sufficient for the purification of FIII-1, FIII-2, and FII, since their SEC and SDS-PAGE profiles represented pure proteins Fraction FI actually contained two isozymes with...
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Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
... strains), C1-6 (strains of Cornell Univ.) and P1-7 (strains from E coli reference center of Pennsylvania State Univ.) b The presence of Stx1 and Stx2 “-” and “+” indicate negative and positive, respectively ... of Verocytotoxinproducing Escherichia coli O157 on Dutch dairy farms J Clin Microbiol 1998, 36, 3480-3487 22 Hitchins AD, Feng P, Watkins WD, Rippey SR, Chandler LA Escherichia coli and the Coliform ... activity of Bifidobacterium longum HY 8001 against Vero cytotoxin of Escherichia coli O157:H7 J Food Prot 2001, 64, 1667-1673 27 Kudva IT, Hatfield PG, Hovde CJ Characterization of Escherichia coli...
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Báo cáo khoa học: "Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus" doc
... citatsomreht a ot detcennoc )nedewS ,aicamrahP( tinu IIrohpitluM eht ni demrofrep saw sisylana citerohportcele ehT la te gnisucof cirtceleosi lacitylanA snoitacifidom thgils htiw ]42[ nibwoT fo dohtem ... ,BKL( rettolb yrd-imes tolBavoN a gnisu demrofrep saw sisylana tolbonummI la te gnittolbonummI ]31[ ilmmeaL yb debircsed snoitidnoc eht ot gnidrocca demrofrep saw siserohportcele dna ,selpmas ,sleg ... noitaraperP )ASU ,daRoiB( lleC balS-lauD II naetorP -iniM eht ni demrofrep saw sisylana citerohportcelE EGAP-SDS ]3[ dohtem drofdarB eht yb detamitse saw sPLG eht fo tnetnoc nietorp ehT fo sPLG...
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Báo cáo y học: "Production, purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virus" pps
... Zhang et al Virology Journal 2010, 7:241 http://www.virologyj.com/content/7/1/241 Page of Figure Expression and purification of the tgK protein M represented standard protein molecular weight ... were injected intradermally with a mixture of 0.5 mg purified His -tagged tgK protein mixed with an equal volume of complete Freund’s adjuvant (Promega) on the back and proximal limbs (100 μl per ... Virology Journal 2010, 7:241 http://www.virologyj.com/content/7/1/241 Page of Figure Purification of the rabbit anti-tgK polyclonal antibody and Western blot assay M represented standard protein...
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Báo cáo khoa học: "Expression, purification and characterization of the Lily symptomless virus coat protein from Lanzhou Isolate" doc
... diagnostics and epidemiologic surveys Methods Strains, plasmids, and enzymes Expression and purification of LSV coat protein Figure Expression and purification of LSV coat protein A, Expression and purification ... Alignment of the amino acid sequence of coat protein of different LSV isolates and other carlavirus Figure Alignment of the amino acid sequence of coat protein of different LSV isolates and other ... any species limitation and with peptide mass tolerance of ± 0.1 Da by using the Mascot search engine The first candidate protein, with a score of 192, was coat protein of LSV isolated from India...
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Inactivation and repair of escherichia coli following UV disinfection influencing factors and photolyase activity
... recovery of E coli ATCC 11229 and ATCC 15597 following h of fluorescent light and sunlight exposure with LP and MP UV disinfection 126 Table 5-2 Comparison of repair rates of E coli ATCC 11229 and ... 2.9.5 Dark repair 58 UV Disinfection and DNA Repair of Escherichia coli 59 2.10.1 UV disinfection of E coli 59 2.10.2 DNA repair of E coli 61 CHAPTER MATERIALS AND METHODS 66 3.1 Overview 66 3.2 ... disinfection and incubation under varying light and temperature conditions 130 Table 6-1 Rate of dimer repair of LP- and MP-UV irradiated photolyase with and without DTT addition 147 xii List of Figures...
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Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
... dismutation of superoxide [32] A least-square fit of Eqn (1) to the data of Fig yields the constants given in the legend of Fig The values of kcat ⁄ Km and kcat for catalysis by C140S– FEBS Journal 273 ... high level expression of foreign genes Gene 176, 269–272 Morimyo M, Hongo E, Hamainaba H & Machida I (1992) Cloning and characterization of the mvrC gene of Escherichia coli K-12 which confers ... method for the protein function of interest In order to construct a selection system for Q143A hMnSOD mutants with enhanced catalytic activity, we took advantage of the reliance of E coli on hMnSOD...
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preparation and characterization of high surface area nanosheet titania with mesoporous structure
... images of the nanosheet TiO2 calcined at 600 and 700 °C showed almost nanoparticles with a mixture of anatase and rutile TiO2 structure (about 10–50 nm in diameter, at 600 °C, Fig 6(g–h)), and rutile ... Isoda and Prof H Kurata, Institute for Chemical Research, Kyoto University for the use of TEM apparatus and to Prof T Yoko, Institute for Chemical Research, Kyoto University for the use of XRD ... loop), indicating the existence of mesopores The pore size distribution of the sample, as shown in the inset of Fig 4, showed that the nanosheet TiO2 with Fig SEM images of the as-synthesized flower-like...
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Báo cáo khoa học: Functional interaction of Escherichia coli heat-labile enterotoxin with blood group A-active glycoconjugates from differentiated HT29 cells docx
... investigated the nature of alternate LT-I receptors in differentiated cells Results Characterization of differentiated HT29 cells In order to analyze the interaction of LT-I with cells that resemble ... cells Effect of CT-B and Helix pomatia lectin Undifferentiated and differentiated HT29-ATCC and HT29-5F7 cells were pre-incubated at °C with 1.0 lM CTB or 10 lM H pomatia lectin and then cells ... Interaction of LT-I with differentiated HT29 cells the number of occupied binding sites of differentiated and control cells were similar We have no clear explanation for this observation and further...
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báo cáo khoa học: " Identification and characterization of the Nonrace specific Disease Resistance 1 (NDR1) orthologous protein in coffee" doc
... amino-acid sequence of CaNDR1a (Figure 4), with residues S189 and G190 being strong ω-site candidates (with P-values of 2.48 × 10 -6 and 2.76 × 10 -5 , respectively) Furthermore, CaNDR1a and its Arabidopsis ... Page of 17 Figure Structural similarities between the Arabidopsis and coffee NDR1 proteins Predicted structural domains and motifs of NDR1 proteins are represented The overall structure of both proteins ... used for transiently expressing HA- and HA-His -tagged CaNDR1a proteins in tobacco leaves (b) Detection of CaNDR1a -tagged proteins by immunoblotting The upper and lower panels show scanned films...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... coimmunoprecipitated with GroEL from 15 and 30 lg of the cell lysate without (lanes and 3) or in the presence of ATP (lanes and 4) was detected with anti-UP12 Ig Samples (3 and lg of total proteins) of the ... (A) Effect of addition of mM DNP on cell growth (h) and expression of UP12 (j) (B) Monitoring of cell growth (s) and amount of UP12 (d) before and after addition of 0.1 mM CCCP (C) Effect of temperature ... level of UP12 expression in E coli cells cultured in various media and under different experimental conditions Cultures of E coli MC4100 were grown at 37 °C in Luria–Bertani, and samples were withdrawn...
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Báo cáo Y học: Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli doc
... truncated fusion protein was puri®ed without any sign of proteolytic degradation Puri®cation The optimized large-scale puri®cation of the fusion protein M-A2aTr316-H10 from 100 g of E coli cells is ... Integrity and stability of the puri®ed protein M-A2aTr316-H10 The puri®ed receptor runs in SDS/PAGE gels as a single, slightly diffuse band with an apparent molecular mass of Fig E coli expressed and ... buer NiA and NiB; s, 0.2% CHS in solubilization buer and 0.02% in buers NiA and NiB The quanti®cation of interactions with speci®c ligands allows the structural integrity and homogeneity of a puri®ed...
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Báo cáo khoa học: Coexpression, purification and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli potx
... production and purification of the mutant protein were the same as those of the wild-type Protein determination and enzyme assay Protein concentrations were determined by the method of Bradford ... assembled in a second round of PCR using oligonucleotides 40 and 41 as primers and cotemplates The PCR product was purified, restricted with SpeI and BamHI, and ligated with SpeI/BamHI-restricted ... this result, because the E coli strain, Epicarian Coli -Codon PlusTM (DE3)-RIL, contains extra copies of the argU, ileY, and leuW tRNA genes The coprecipitation of OraS and OraE might imply that...
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Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
... 58 kD ( the sum of 55 + 3.4kD of MCoTI-II) As seen on Fig 10 the 58 kD protein band was found in induced sample as the major band, acounted for 80% of total protein (lane 1,3,5 and 6) 58 kD Fig ... growth and mortality of insect pests, Plutella xylostella (PX) and Spodoptera litura (SL).17th International Congress of Biochemistry and Molecular Biology Annual Meeting of the American Society of ... U.K.,Cleavage of structure proteins during the assembly of the head of bacteriophage T4, Nature V.227 (1970) pp 680-685 10 Laurent Chiche, A Heitz, J C Gelly, J.Gracy, T.C Pham, T H, Phan, J F Hernandez and...
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Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the specificity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf
... Activity and specificity of Fpg and OGG1 in buffers with different concentrations of KCl and MgCl2 (A–C) Fpg, (D–F) glycosylase activity of OGG1, (G–I) AP lyase activity of OGG1 The extent of cleavage ... of ionic strength and Mg2+ on the activity and specificity of OGG1, we measured the apparent values of k2 and k3 under conditions of low salt (KPi only) and no Mg2+, low salt and 20 mm Mg2+, and ... Activity and specificity of Fpg and OGG1 in buffers with different concentrations of KGlu and MgCl2 in the presence of KPi (A–C) Fpg, (D–F) glycosylase activity of OGG1, (G–I) AP lyase activity of OGG1...
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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
... interaction with one of the manganese ions This, together with the fact that the positions of other Fig Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and ... with and without a previous incubation with mM Mn2+ for 20 at 37 °C Activities are expressed as percentage of the corresponding control not preincubated with Mn2+ and assayed in the absence of ... thermostated at 22 °C CD spectra of wild-type and D153N mutant enzymes (5.5 lM) were measured in the range 200–250 nm, with a bandwidth of nm and a scan speed of 50 nmÆmin)1 The buffer solution...
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Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
... vector, were used: E coli K-12 AG1 for pCA24N–Pta; E coli BL21(DE3) for pET28–F1 and pET28–F2; and E coli M15 for pQE30–F3 Construction of the E coli acs pta deletion strain The E coli acs pta deletion ... Journal compilation ª 2010 FEBS 1959 Escherichia coli phosphotransacetylase V A Campos-Bermudez et al Fig Comparative CD spectra of E coli Pta and truncated Ptas CD spectra of Pta, Pta-F1 and ... obtained for Pta-F2 and Pta-F1 (B) Activation of the acetyl phosphate synthesis activity of E coli Pta by different concentrations of pyruvate and PEP Results are presented as percentage of activity...
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ANTIBIOTIC RESISTANCE OF ESCHERICHIA COLI ISOLATED FORM POULTRY WORKERS, PATIENTS AND CHICKEN IN THE EASTERN PROVINCE OF SAUDI ARABIA ppt
... Standards (NCCLS 1993) In addition, E coli organisms isolated from chicken and Table Comparison of pattern of resistance of E coli isolates from chicken and patients E coli- chicken isolates E coli- patient ... resistance of Escherichia coli isolates from the faecal flora of veterinarians with different professional specialties Veterinarians’ Quarterly 17, 146–149 Burkhanova KHK (1980) Properties of E coli ... References Allan BJ, Van-den-Hurk W & Potter AA (1993) Characterization of Escherichia coli isolated from cases of avian colibacillosis Canadian Journal of Veterinary Research 57, 146–151 Allen DG, Pringle...
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Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot
... assignment of H-7a/b and C-7 of L,D-Hepp residues F and H The chemical shift of H-6 of residue F could also be assigned in the latter spectrum The chemical shift of the H-6 proton of residue ... of anomeric protons and two pairs of signals originating from 3-deoxy protons characteristic of aKdop The assignment of H and 13C-resonances (Tables and 3) and determination of vicinal coupling ... chemical shift values MATERIALS AND METHODS Bacteria and bacterial LPS E coli strains 2513 and F653 were cultivated and used for the isolation of LPS by phenol/chloroform/petroletherextraction as...
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Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf
... Overproduction and molecular characterization of the modied variants of E coli CMP kinase The wild-type and various modied forms (S36A, R110M, D132A, D132H, D132N, D132S and R188M) of E coli CMP kinase ... NMPs (A37 and T39 in these enzymes are equivalent to S36 in E coli CMP kinase) Substitution of R110 with Met in E coli CMP kinase affected both kcat and Km The kcat Km ratio with CMP and dCMP ... catalysis Kinetic and nucleotide-binding properties of E coli CMP kinase variants with ATP as variable substrate Determination of the reaction rates of different modied CMP kinases with ATP as the...
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