chất lượng đào tạo nghề của huyện qua đánh giá của người lao động
Epigenetic regulation of normal and malignant hematopoiesis
... following the trimethylation of H3K27 by PRC2 or PRC3, which serves as a marker for PRC1 binding (Squazzo et al., 2006) PRC1-binding blocks the activating function of the ATP-dependent remodeling ... methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex Nature 393: 386–389 O’Geen H, Squazzo SL, Iyengar S, Blahnik K, Rinn JL, Chang HY et al (2007) Genome-wide analysis of KAP1 binding ... Target-based approach to inhibitors of histone arginine methyltransferases J Med Chem 50: 2319–2325 Squazzo SL, O’Geen H, Komashko VM, Krig SR, Jin VX, Jang SW et al (2006) Suz12 binds to silenced...
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Đề tài " Approximating a bandlimited function using very coarsely quantized data: A family of stable sigma-delta modulators of arbitrary order " pptx
... Σ∆ quantization is much cheaper to implement than binary quantization of less frequent samples However, the two algorithms behave very differently when imperfections, in particular imperfect quantizers, ... which would make such quantizers very expensive in practice (or even impossible if τ is too small) On the other hand [3], Σ∆-quantizers are robust under such imperfections of the quantizer, allowing ... it is possible to construct stable Σ∆-quantizers of arbitrary order The quantizers (36) are, however, very far from schemes built in practice for 1-bit Σ∆-quantization Often, such practical schemes...
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Báo cáo y học: " Reduced levels of two modifiers of epigenetic gene silencing" ppsx
... was tested in an open field (40 cm × 40 cm) Mice were scored for the number of 10-cm2 squares entered (Squares) and the number of times they reared on their hind legs (Rears) in a 2minute period ... TotalPrep RNA Amplification kit (Ambion, Carlsbad, CA, USA) Amplified cRNA was assessed for quantity and quality also using the Agilent Bioanalyzer 2100 RNA was hybridized to MouseRef-8 v2.0 Expression ... a 40 cm × 40 cm box with a grid dividing it into 16 squares (10 × 10 cm) Mice were placed in the open field and scored for the number of squares entered, and number of times the mouse reared...
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Báo cáo y học: "Reduced levels of two modifiers of epigenetic gene silencing, Dnmt3a and Trim28, cause increased phenotypic nois" pdf
... was tested in an open field (40 cm × 40 cm) Mice were scored for the number of 10-cm2 squares entered (Squares) and the number of times they reared on their hind legs (Rears) in a 2minute period ... TotalPrep RNA Amplification kit (Ambion, Carlsbad, CA, USA) Amplified cRNA was assessed for quantity and quality also using the Agilent Bioanalyzer 2100 RNA was hybridized to MouseRef-8 v2.0 Expression ... a 40 cm × 40 cm box with a grid dividing it into 16 squares (10 × 10 cm) Mice were placed in the open field and scored for the number of squares entered, and number of times the mouse reared...
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Báo cáo y học: "Evaluating dosage compensation as a cause of duplicate gene retention in Paramecium tetraurelia" pot
... the remaining duplicate will now be positively selected for The first part of this prediction is qualitatively satisfied by the observations from the P tetraurelia genome of the retention of genes ... retain duplicate genes in such high numbers as dosage compensation appears to be able to Eventually, quantitative models characterizing these various processes can be tested against the data to extend ... that the negative selective pressure for retention of each duplicate in a pathway would not be equally strong Interestingly, it has been argued that the neutral expectation for biological networks...
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Báo cáo y học: "Dynamic cumulative activity of transcription factors as a mechanism of quantitative gene regulation" docx
... of target genes (T1,T2 and T3) Figure used for quantification study of combinatorial gene regulation in a convergence mode Scheme Scheme used for quantification study of combinatorial gene regulation ... in 152 motifs The Yates chi-square test has been used to determine the difference between the success frequencies of the FFL and non-FFL groups The results (chi-square = 0.44, df = 1, P = 0.507) ... (additive) mode that is assumed in equation (see Materials and methods) is not fulfilled in such cases Given regulators might exhibit some similarities in quantitatively regulating the transcription...
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Báo cáo sinh học: " A trial of somatic gene targeting in vivo with an adenovirus vector" pdf
... 1):S164-171 Vrancken Peeters MJ, Perkins AL, Kay MA: Method for multiple portal vein infusions in mice: quantitation of adenovirusmediated hepatic gene transfer Biotechniques 1996, 20:278-285 Nakatani...
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A study of Stable models of stock markets
... coefficient (Table and Figure 6) for the Hurst exponent illustrates the adequacy of estimation Since we need a high adequacy, we take the Hurst estimates only with the correlation over 0.9 (Figure ... indexes in third and fourth rows are similar (theoretically they should be equal) The average absolute difference is equal to 0.132 (min 0.004 and max 0.27) In these cases (Figure 9), correlation ... , here k=1, 2…[N/m] m i =( k −1 )m +1 ∑ Self-similarity is often investigated not through the equality of finite-dimensional distributions, but through the behavior of the absolute moments Thus,...
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Báo cáo khoa học: Gene expression silencing with ‘specific’ small interfering RNA goes beyond specificity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx
... allowed quantification of the local background and evaluation of the array homogeneity, which is taken into account in the normalization Furthermore, in order to consider the purity and quality ... TBP ⁄ TFIID was used as the reference gene for normalization and relative mRNA steady-state level quantification Melting curves were generated after amplification, and data were analyzed using the ... peroxidase-conjugated anti-(mouse IgG) (Dako, Glostrup, Denmark) and an ECL system (Amersham) Equal protein loading was checked by the immunodetection of a-tubulin Low-density DNA microarray...
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báo cáo khoa học: "Atypical epigenetic mark in an atypical location: cytosine methylation at asymmetric (CNN) sites within the body of a non-repetitive tomato gene" ppsx
... associated with H3K27me3, a major repressive mark for gene expression Such a covalent modification quantitatively appeared to decrease with water stress (p < 0.05) (Figure 7) In contrast, H3K4me3, ... performed using Dynabeads protein A (Invitrogen) and anti-H3K4me3 or anti-H3K27me3 antibodies (Abcam) Quantitative Real-Time PCR was carried out as indicated in the Methods section Comparison between ... Figure Asr1 is induced upon stress Water-stress time course of Asr1 leaf mRNA steady-state levels quantified by real-time RT-PCR Actin mRNA (considered a constitutive transcript) was measured at...
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Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx
... epitope tags may constitute a measurable perturbation HA is less charged than His6, but a precise quantitative difference between these two tags remains to be established We believe that such discrepancies,...
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..GENE CLONING AND DNA ANALYSIS..GENE CLONING AND DNA ANALYSISAn IntroductionT.A. BROWNFaculty of Life Sciences University of Manchester ManchesterSixth EditionA John Wiley & Sons, Ltd., Publication. pdf
... PCR enables the amount of starting material to be quantified 158 9.4.1 Carrying out a quantitative PCR experiment 159 9.4.2 Real-time PCR can also quantify RNA 160 Contents xii Part II The Applications ... the gene cloning revolution was at its height, it hardly seemed possible that another, equally novel and equally revolutionary process was just around the corner According to DNA folklore, Kary ... important development of the last few years has been the introduction of real-time PCR as a means of quantifying the amount of a particular DNA sequence present in a preparation This technique is now...
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Elite Leadership: A collection of Leadership articles By Mark Wager doc
... skills Performance issues will not sort themselves out Courage is not a desirable quality of a leader, it’s an essential quality When I coach new Managers I often hear about the challenges they have ... have quality Managers; the kind of people who are committed technically, knowledgeable, task/process focussed individuals Yet the truly elite organisations have something more, an extra quality ... requires both managerial and leadership skills in order to perform their role adequately but it’s the leadership qualities that allow teams to perform at the truly elite level If you are still...
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Báo cáo khoa học: Improvement of a monopartite ecdysone receptor gene switch and demonstration of its utility in regulation of transgene expression in plants pdf
... (Fig 7) Quantitative RT-PCR (qRT-PCR) analysis of methoxyfenozide-inducible AtZFP11 expression level To further analyze methoxyfenozide-inducible AtZFP11 expression, AtZFP11 mRNA levels were quantified ... transcript levels from different transgenic plants, the average starting quantity of AtZFP11 was normalized to the average starting quantity of the a-tubulin gene, which is assumed to be at a constant ... AtZFP11 transcript in each sample was calculated using a standard curve (logarithm of the starting quantity versus threshold cycle) generated for AtZFP11– pGEM-T Easy plasmid dilutions by the iCylcer...
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Báo cáo khoa học: Loss of sense transgene-induced post-transcriptional gene silencing by sequential introduction of the same transgene sequences in tobacco pot
... However, in S44-end-2 leaves, the level was reduced in comparison with that of S44 plants (Fig 3B) Quantitative real-time RT-PCR analysis indicated that both transcripts for the NtFAD3 transgene ... incidence of irregular processing of transcripts, or RDR6 may not be able to recognize such a low quantity of transgene mRNA in S44-end plants 1700 Our results indicate an example in which the transgene ... Netherlands) The primers used and the amplified regions are shown in Table S1 (see Supporting information) Quantitative RT-PCR Total and nuclear RNAs were prepared from S44 and S44-end-2 plants grown at...
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Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx
... and sensitive method for Identification of a basic glycoprotein induced by ethythe quantitation of microgram quantities of protein utillene in primary leaves of azuki bean as a cationic perizing ... C roseus 35 Frangioni JV & Neel BG (1993) Solubilization and puriperoxidase gene from Senecio squalidus L (Asteraceae) fication of enzymatically active glutathione S-transferase Plant Mol Biol ... protein was electrophoresed in 12% SDS ⁄ PAGE [38] Samples (20 lg of each) were boiled for 10 in an equal volume of · SDS ⁄ PAGE sample buffer with 0.2 m dithiothreitol 26 Insoluble materials were...
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Báo cáo khoa học: The effect of ST2 gene product on anchorage-independent growth of a glioblastoma cell line, T98G doc
... in meningiomas Ó FEBS 2003 Fig Real-time quantitative PCR analysis of ST2 gene expression in meningiomas and glioblastomas Distribution of the relative quantity of ST2 mRNA of brain tumor samples ... 2, A172; lane 3, U251; lane 4, U373Mg; lane 5, T430; lane 6, T98G; lane 7, Onda11 (B) Relative quantities of ST2 mRNA to TM12 are shown All experiments were performed in duplicate and repeated ... level of GAPDH A normalized ST2 value of TM12 was taken as the standard, and the final relative quantity of ST2 mRNA was expressed relative to the standard Error bars designate the standard deviation...
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Báo cáo khoa học: Cloning of a rat gene encoding the histo-blood group A enzyme Tissue expression of the gene and of the A and B antigens potx
... of pathogens and the presence of protective antibodies mean that all individuals would not be equally sensitive to a given pathogen This would provide a mechanism of protection against the pathogenic ... of 94 °C 30 s, 64 °C 45 s, 68 °C The amplification yields a product of 929 bp The control of cDNA quality was performed by amplification of glyceraldehyde phosphate dehydrogenase (GAPDH) Transfection ... which are characterized by their ability to transfer galactose and N-acetylgalactosamine about equally well [16] That the rat enzyme described here is truly an A enzyme is confirmed by the fact...
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Báo cáo hóa học: " Prevention of hyperglycemia-induced myocardial apoptosis by gene silencing of Toll-like receptor-4" potx
... proteins) were incubated with 10 μl of DCF-DA (10 μM) for h at 37°C The fluorescent product formed was quantified by spectrofluorometer at the 485/ 525 nm Changes in fluorescence were expressed as an ... signal (arrows) from (a) sham treated mice (n = 6) or (b) STZ-treated diabetic mice (n = 6) (C) Quantification of TUNEL positive cardiomyocytes (D) Fas expression in the hearts of STZ mice Diabetes ... retrieved Total mRNA was extracted and used to detect the TLR4 transcripts by qPCR The relative quantity of TLR4 mRNA was expressed as mean ± SD (*) Statistical significance when compared with...
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Báo cáo sinh học: " Soilborne wheat mosaic virus (SBWMV) 19K protein belongs to a class of cysteine rich proteins that suppress RNA silencing" docx
... sucrose, 10% glycerol, and 10 mM β-mercaptoethanol) Extracts were centrifuged at 10,000 g for 10 Equal volumes of supernatants and protein loading buffer (2 % SDS, 0.1 M dithiothreitol, 50 mM Tris-HCl...
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