... concentration of 1.25 · 105 CFUÆmL)1 for each strain in a final volume of 500 lL The actual CFUs of each strain in both inocula were determined by plating serial dilutions on 7H11 plates containing either ... (DIM-less) and PMM74 (DIM A-less) mutant strains to that of the wild-type strain Mice were infected intranasally either with a mixture of strains H37Rv:pMV361H and PMM74 or with a mixture of strains ... H37Rv:pMV361H inoculum, as determined by growth on 7H11 plates containing either kanamycin (Km) or hygromycin (Hyg) Mice were killed or 21 days postinfection, and the mutant and wild-type loads in lungs and...
... and j Depending upon the directions of spin-spin interaction, spin chains can be classified into three types: Heisenberg spin chain, XY spin chain and Ising spin chain In the following, we will ... dynamics of spin chains viii List of Publications Vinitha Balachandran, and Jiangbin Gong, Adiabatic quantum transport in a spin chain with a moving potential, Phys Rev A 77, 012303 (2008) Vinitha ... STUDIES OF QUANTUM SPIN CHAIN DYNAMICS ANDTHEIRPOTENTIAL APPLICATIONS IN QUANTUM INFORMATION VINITHA BALACHANDRAN (M Sc., Cochin University of Science And Technology, India) A THESIS...
... manufacturers and other active classes of the minority of our voters may influence; and only twice in our history, from 1789 to 1808, and from 1846 to 1860, have enough of the minority found their interests ... because of the obviously unusual cause of sudden and enormous calling inof government bonds, the basis of bank-note issue.] and a very small reserve in specie and legal-tender notes and poor and ... secure and retain the attention of readers of this class of books This same conciseness has also been imposed on our author by the inherent dryness and minuteness of his faithful inquiry into hundreds...
... expression of a number of genes involved in the pathogenesis of OA, including IL-1β, TNF-α, MMP-1, MMP-13, inducible nitric oxide synthase, and microsomal prostaglandin E synthase-1 [25-28], and is ... each of aprotinin, leupeptin, and pepstatin, 1% NP-40, mmol/l Na3VO4, and mmol/l NaF) Lysates were sonicated on ice and centrifuged at 12,000 rpm for 15 minutes The protein concentration of the ... specificity of staining was confirmed using nonimmune control IgG (Figure 2c, f) These observations demonstrate the in vivo expression of 15-LOX-1 and -2 proteins in OA cartilage 13-HODE and 15-HETE inhibited...
... http://www.biomedcentral.com/1471-2229/11/5 Page 13 of 16 Figure GO analysis oftargetsof known and new miRNAs in this study Blue bars indicate the enrichment of miRNA targetsin GO terms Green bars indicate the percentage of total annotated ... Station of the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, in Beijing in May Seeds from soybeans 15 days after flowering (DAF) were collected and quickly frozen in ... specifically expressed in seeds Identification of the corresponding target genes and study oftheir roles will elucidate possible functions of miRNAs and target genes in relevant processes of seed development...
... protein kinases B (PKB) plays an important rolein mammalian cellular signaling Ara: α-L-arabinopyranosyl B16F10: Murine melanoma cancer cell line BrdU: Bromodeoxyuridine BSA: Bovine serum albumin ... Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples of soyasapogenol B measured in SIM mode with m/z values of 457.4 (negative) and panel ... B, E) and are found in various glycoside forms Soyasaponins A and soyasaponins B are the most abundant type of saponins found in soybean (Glycine max (L.)) and related products The saponin content...
... tenured Professor in 2006 He earned his B.S in Mathematics and Agricultural Engineering in India, a M.S degree in Food Engineering in Thailand, and completed his Ph.D in Agricultural Engineering from ... Bioprocess Engineering in the Department of Agricultural and Biosystems Engineering, South Dakota State University, Brookings, SD, USA He joined the faculty in 1997 as an Assistant Professor and was ... universities in SD, USA - SD School of Mines and Technology (Rapid City, SD, USA), and SD State University (Bookings, SD, USA) He holds a Masters degree in Chemical Engineering and a PhD degree in Biotechnology...
... chlorinated POPs) causing a small but significant increased risk of TDS disorders, but not being the major sole influence explaining the majority of cases In view of the effects in rats of certain ... trend is perhaps also emerging in Europe, as TGCT 13 incidence is increasing in Finland (where the incidence has been low) but appears to be plateauing or even declining in Denmark (Moller 2001; ... but again the percentage of cases arising because of TDS remains uncertain Even if it is accepted that many cases of TDS disorders have their origins in fetal life, identifying the causes of TDS...
... result in the ubiquitination of MBP–TRIM5a, whereas the incubation of MBP–TRIM5a in the reaction mixture containing UbcH5B resulted in both the monoubiquitination and polyubiquitination of MBP–TRIM5a ... Ubiquitination of TRIM5a and its role K Yamauchi et al A B Fig E3 activity of wild-type TRIM5a and its RING mutant in vitro (A) Schematic representation of the RING-finger domain of TRIM5a The amino ... structure of TRIM5a and Ro52 As shown in Fig 1C, both proteins possess RING-finger and B-box domains in the N-terminal region In the central region, Ro52 contains two separated coiled-coil domains,...
... image of the double immunofluorescent assay The expression of CD68+ (green) and CD147+ (red) cells, and the co-expression of CD68+CD147+ cells (yellow) in the lining and sublining layers of rheumatoid ... immunohistochemical staining and immunofluorescence were performed The immu- noreactivity of CD147 was more intense and more widespread in RA synovium than in OA and AS synovium, and the expression of CD147 ... 37°C in a humidified atmosphere of 5% CO2 The cells remaining in the upper compartment were completely removed with gentle swabbing The filter was fixed and stained with haematoxylin and eosin...
... components of the cartilage ECM including dermatopontin (DPT), asporin (ASPN), biglycan (BGN), cartilage intermediate protein (CILP2), fibromodulin (FMOD), tenascin C (TNC) and fibronectin (FN1) ... significant increase in expression (3.3 to 67fold) during 3D culture The expression of different genes coding for ECM degrading enzymes, such as a desintegrin and metalloproteinase with thrombospondin ... design and coordination of the study and drafted the manuscript JR participated in gene expression data processing, study design and coordination AL and MS conceived the study and participated in...
... [27] Of note, Hfe-/- D2 mice also have increased duodenal expression of mucin and spermine synthase Increased binding of Dmt1 to mucin in vesicles near the intestinal surface was observed in iron-deficient ... was recently found to be involved in the control of hepcidin expression [23], in the B6 strain Finally, and worthy of note within the context of modifiers of iron loading severity, wildtype D2 ... observed and is interesting within the context of recent reports indicating that Zip4 is a minor intestinal iron importer [26] and that Zip14 mediates non-transferrin-bound iron uptake into cells...
... understanding of origin of red-eared sliders and legislation against the release of wildlife .180 6.3.5 Perspectives and opinions regarding the release and feeding of turtles in Singapore ... bars) of time spent participating in a) swimming slowly and/ or remaining stationary, b) swimming fast, c) basking, d) courtship, e) cloacal sniffing, f) swimming along the bottom, g) feeding, h) intraspecies ... carried out in Asia on non-indigenous terrapins, including impacts of red-eared sliders on indigenous species, although there is ongoing research in Singapore 1.4 Environment of Singapore Singapore...
... Perspective of the Protein–RNA Interactions Involved in Virus-induced RNA Silencing and Its Suppression Summary RNA silencing regulated by small RNAs, including siRNAs, miRNAs, and piRNAs, results in ... protein complexes indicated that most of human AGO1 and AGO2’s partner proteins are RNA binding proteins [74] The interactions between AGOs andtheir partner proteins can be either direct protein-protein ... understanding of Argonautes Typical Argonaute proteins consist of four distinct domains from N-termini to Ctermini: N-terminal, PAZ, Mid and PIWI domains [68] (Figure 1-4A) 2.3.5.1 PAZ domain PAZ...
... fixed in buffered formalin, embedded in paraffin, and stained with hematoxylin-eosin-safran and Masson's trichrome Hepatic steatosis, stage of fibrosis and grade of disease activity were determined ... significance of liver biopsy in determining the causes of high ALT levels The most important limitation of this study is the lack of long-term follow-up and evaluation of response to antiviral therapy in ... the predictors of clinical and pathological characteristics in these patients with steatosis andtheir diagnostic values in hepatic fibrosis Patients and methods Patients A total of 204 HBeAg-negative...
... hSGLT1, suggesting that differences in the aglycon binding site play an important rolein the inhibitory effect of this substance In contrast, differences in the sugar binding site resulted in an preferen- ... metformin), to reduce insulin resistance (using glitazones like rosiglitazone and pioglitazone) and/ or to control hepatic glucose release (using biguanides) [7] In addition, manipulation of insulin ... phosphate-buffered saline (PBS; Invitrogen, Karlsruhe, Germany) and then incubated with 0.1 ml of a solution containing µM CC2-DMPE and 0.02% pluronic acid in PBS After in- cubation in the dark for...
... period in 2005, mostly winter, was stable Fig shows the changes in TOC and DOC concentrations with the change of flow in 2005, and Fig shows the changes in TOC and T-COD loadings with the change of ... events and storm runoff events which bring increases in pollutant loads during flooding-stages in large river catchments As the traverse water mixing in the wide Yodo River was relatively weak during ... differences in traveling time of river water from their main pollutant sources and the lack of uniformity of rainfall distribution in the broad catchment It was particularly evident that the values and...
... overexpression of a-synuclein significantly affected the levels of four proteins [stathmin 1, glutathione S-transferase 4912 d Not applicable (GST)p, Ran binding protein and C1q binding protein], related ... (Tables S2 and S3) In both cases, bioinformatic analysis revealed that the NF-jB pathway could be involved in determining the effects of dopamine treatment and a-synuclein overexpression Accordingly, ... Levels of the RAN binding protein were reduced in a-syn cells with respect to b-gal cells and RuvB-like is upregulated in a-syn cells in the absence of dopamine CRMP4, a member of a family of neuron-enriched...
... the interaction with the PDZ proteins: Mint-3, AMPA binding protein and glutamate receptor interacting protein (GRIP) It would therefore be interesting to determine whether this motif is also involved ... domain In HT1080, the intracellular trafficking of MT1/EYFP was indistinguishable from that of wild-type MT1-MMP and both constructs were found to accumulate in the Golgi apparatus and the TGN in ... Roghi et al Roleof GRASP55 in MT1-MMP activation l l l l l l l l l l l l l MINT-7897617, MINT-7897659, MINT-7897681, MINT-7897702, MINT-7897725, MINT7898032, MINT-7898011, MINT-7897907, MINT-7897884:...