... pharmacological management of above mentioned diseases and symptoms, to discover effective, alternative antiinflammatory reagents is still in need Several Chinese herbal medicines have anti-bacterial and ... Ultimately it is hoped that BAI will be a possible candidate for future development of novel antiinflammatory therapies In this study, we examined effects of BAI on the production of important ... the expression of inflammatory cytokines indicates its usefulness in the development of novel antiinflammatory therapies List of abbreviations BAI, Baicalein EMSA, electrophoretic mobility shift...
... near the N terminus [2] Based on this derived structure, HAT is thought to be a member of the type -II transmembrane serine protease family, which includes corin, enteropeptidase, MT-SP1 (also known ... protease J Biol Chem 273, 11895–11901 Hooper JD, Clements JA, Quigley JP & Antalis TM (2001) Type II transmembrane serine proteases-insights into an emerging class of cell surface proteolytic enzymes...
... Puttonen S, Kivimäki M, Elovainio M, Pulkki-Råback L, Hintsanen M, Vahtera J, Telama R, Juonala M, Viikari JS, Raitakari OT, KeltikangasJärvinen L: Shift work in young adults and carotid artery intima-media...
... patients with chronic bronchitis Am J Respir Crit Care Med 1994, 149(5):1311-6 Iikura M, Takaishi T, Hirai K, Yamada H, Iida M, Koshino T, Morita Y: Exogenous nitric oxide regulates the degranulation ... manufacturer's instructions First-strand cDNA was synthesized from µg total RNA with SuperScript II using Oligo (dT) as primers (Invitrogen, Carlsbad, CA, USA) PCR was performed on the reverse...
... cultured regional lymph nodes from another patient with lung cancer (48year-old man, T4N2M0, stage IIIB adenocarcinoma) in IL2 for month The significant cytotoxic activity of the activated killer ... 51Cr-releasing test One million tumor cells were labeled with 3.7MBq sodium chromate-51Cr (Na251CrO4; Daiichi Radioisotope Lab Ltd., Tokyo, Japan) for 45 minutes, washed three times with culture medium ... intraperitoneal chemohyperthermia in abdominal cancers with peritoneal carcinomatosis: a phase II study J Clin Oncol 2003, 21(5):799-806 Sugarbaker PH: Strategies for the prevention and treatment...
... EcoRI, BamHI and SmaI sites, respectively The 3¢-antisense primers contained XhoI, BamHI and HindIII sites, respectively, in addition to a stop codon Preparation of cDNAs encoding N-terminal deletion ... assay protocol supplied with the BacVector-2000 kit Sf9 cells were routinely propagated in Sf-90 0II serumfree medium (Invitrogen, Groningen, the Netherlands) Several purified plaques were examined ... each purified plaque at a multiplicity of infection (MOI) of 5–10, and cultured in 2.5 mL of Sf-90 0II serum-free medium for 48 h at 27 °C The culture supernatant fraction was recovered, and 500 lL...
... acid-releasing functions of the three heparin-binding group II subfamily enzymes (IIA, IID and V) in HEK293 transfectants, we noted that sPLA2IID released arachidonic acid after A23187 stimulation ... presence of SCF and IL-6 from cord blood cells [38] expressed only sPLA2-IID, but not the other sPLA2s including -IB, -IIA, -IIE, -IIF, -V and -X (Fig 5) The expression of cPLA2a was readily detected ... mast cells was subjected to RT-PCR (30 cycles) using specific primers for human sPLA2-IB, IIA, IID, IIE, IIF, V and X (left) and for cPLA2a (right) After staining of the gel with ethidium bromide,...
... FasL promoter (5¢-ATTGTGGGCGGAAACTTCCAG-3¢) with additional GATC motifs at the 5¢ end into the BglII site of the pGL2-promoter (Promega, Madison, WI) that carries an SV40 promoter The eukaryotic...
... metastasis Since IIIB is a locally advanced stage and the potential effects of mast cells may be stronger in earlier stages of colon cancer development such as stage I, stage II and their function ... Survival analysis with univariate analysis By the end of the 5-year follow-up, 66 patients with stage IIIB colon carcinoma were alive, so the 5-year survival rate was 70.9% Based on univariate analysis, ... cancers, the mixture of TNM stages, and small sample sizes [15-19] This study analyzed 93 stage IIIB colon cancer patients to avoid those shortcomings The results show that, although the mast...
... signalregulated kinases 1/2 control claudin-2 expression in MadinDarby canine kidney strain I and II cells J Biol Chem 2005, 280:3780-3788 Li XD, Lankinen H, Putkuri N, Vapalahti O, Vaheri A: Tula...
... metastasis Since IIIB is a locally advanced stage and the potential effects of mast cells may be stronger in earlier stages of colon cancer development such as stage I, stage II and their function ... Survival analysis with univariate analysis By the end of the 5-year follow-up, 66 patients with stage IIIB colon carcinoma were alive, so the 5-year survival rate was 70.9% Based on univariate analysis, ... cancers, the mixture of TNM stages, and small sample sizes [15-19] This study analyzed 93 stage IIIB colon cancer patients to avoid those shortcomings The results show that, although the mast...
... expression was quantified by real time PCR Reactions were carried out using a Smart Cycler version II (Takara Bio, Inc., Japan) with a SYBR ExScript RT-PCR kit (Takara Bio, Inc., Japan) PCR was started ... expression in adipocytes of C57BL/6 mice Biochem Biophys Res Commun 2005;329:1127–1132 [23] Shimba S, Ishii N, Ohta Y, Ohno T, Watabe Y, Hayashi M, Wada T, Aoyagi T, Tezuka M Brain and muscle Arnt-like...
... fluorogenic substrates J Biol Chem 1987;262:1363–73 106 Silver R, Silverman AJ, Vitkovic L, Lederhendler II Mast cells in the brain: evidence and functional significance Trends Neurosci 1996;19:25–31 107 ... Perdue M, et al Pavlovian conditioning of rat mucosal mast cells to secrete rat mast cell protease II Science 1989;243:83–5 112 Theoharides TC, Singh LK, Boucher W, et al Corticotropin-releasing ... suppression of contact hypersensitivity responses in mice J Exp Med 1998;187:2045–53 124 Alard P, Niizeki H, Hanninen L, Streilein JW Local ultraviolet B irradiation impairs contact hypersensitivity...
... anti-mouse CD16/CD32 (Fc␥III /II receptor) monoclonal antibody Coaggregation of Fc⑀RI with Fc␥RIIB in Mast Cells — Ekoff et al Figure Expression of Bim induced by Fc⑀RI and Fc␥RIIB or by Fc⑀RI alone ... threonine; 2.4G2 Ab = anti-mouse CD16/CD32 (Fc␥III /II receptor) monoclonal antibody Fc⑀RI-Induced Expression of Bim Is Not Inhibited by Coaggregation with Fc␥RIIB A key regulator of apoptosis is the ... coaggregation of Fc⑀RI with Fc␥RIIB (see Figure 1B) Results Phosphorylation of Akt Is Attenuated by Coaggregation of Fc⑀RI with Fc␥RIIB Coaggregation of Fc⑀RI with Fc␥RIIB Inhibits IgE-Dependent Mast-Cell...