... change ofthe electrostatic potential, etc Kind and strength ofthe formed bonds between metal and ligand depend onthe ionisation potential ofthemetal ion, its electron affinity and the donor properties ... Characteristic of both investigated systems is the strong compression of hydrogel volume with increasing amount ofthemetal ion Attention must be paid to the fact ofthe influence ofthe degree of macroligand ... macroligand ionization onthe character ofthe conformational change ofthe linear segments ofthe gel It is seen that under high pH the swelling coefficient ofthe PEI gel passes through a maximum in the...
... solutions were prepared The first was the gel-filtration elution buffer containing the 43-kDa protein incubated for 30 at room temperature; and the second was the gel-filtration elution buffer containing ... Representative surface plasmon resonance sensorgrams ofbinding between the 43-kDa protein and the 4-kDa peptide were dependent on concentration Details ofthe procedure are described in the Materials ... the function of these molecules is to bind to their target proteins to regulate or inhibit their activities, it is assumed that the function ofthe 4-kDa peptide also relates to the regulation...
... FACSCalibur (Becton-Dickinson, San Jose, CA, USA) Surface biotinylation of proteins To analyze the stability of CD38 onthe plasma membrane ofthe different Ba/F3 mutants, labeling ofthesurface proteins ... homodimers (data not shown) These results suggest that the stabilization of CD38 homodimers by crosslinkers depends strongly onthe conformation and orientation between the CD38 monomers It has been ... extended expression onthe plasma membrane Enzyme activity is not dependent onthe presence of stable homodimers As the mutations in the cytoplasmic region and the a1-helix of CD38 affected dimer...
... contributions YC contributes to the conception and design, the conduction ofthe experiment, the collection, analysis and interpretation of data, the drafting the manuscript and acquisition of ... also synthesized and secreted in other parts ofthe body such as in the peritoneal cavity and joints [4,5], where its adsorption onthe surfaces of tissues at these sites has been demonstrated ... in the lung regions ofthe body The present results offer further support to the Page of (page number not for citation purposes) Journal of Orthopaedic Surgery and Research 2007, 2:14 opinion...
... polarized along the direction ofthe vector (π × z), the magnetic field bends the trajectory ofthe electrons, resulting in a rotation ofthe spin ofthe transmitted electrons The reflected electrons suffer ... trajectories ofthe electrons and the outgoing electrons will propagate in the same direction as the incident ones The spin ofthe reflected electron, with reversed longitudinal wavevector and conserved ... barrier onthesurfaceof a three-dimensional topological insulator We found that (1) the propagating behavior of electrons in such system exhibits a strong dependence onthe direction ofthe incident...
... infection in ambient air fumigation The fumigation with higher ozone concentrations could be sterilizing The effect ofthe ozone fumigation and fungal infection onthe epicuticular wax structure seemed ... study The ozone concentration used in the experiment carried out by Schmitt et al (1987) was too low (100 ,ug) to cause significant surface erosion in the material analyzed in our study Ozone concentrations ... the needles is interesting at least as an intensifying factor in the erosion caused by other air pollutants In the current study, thesurface in the needles infection in ambient air fumigation...
... impact on improving the laboratory diagnosis of prosthetic hip joint infections Conclusion A wide range of bacteria can be found onthesurfaceof prosthetic hip joints removed at revision arthroplasty ... methods One ofthe major problems in accurately determining the infection rate is the difficulty in isolating, by traditional culture methods, the bacteria from thesurfaceofthe prosthetic hip ... culture ofthe same samples The clinical interest of this study is the presence of any organism onthe prosthetic hip joints and the role, if any, that they have in initiating, pro- Page of 11...
... of this epitope onthesurfaceof this HIV-1 subtype B However, this study is preliminary and further experiments will be required to confirm these observations Conclusion The differences amongst ... http://www.jibtherapies.com/content/7/1/5 Peptides corresponding to conserved neutralizable epitopes on C1 and C2 regions of gp120 (HIV-1 CRF01_AE) The designation of peptides corresponding C1 (C1E1 ... nm The cutoff is defined as the mean value of absorption of serum samples from mice immunized with normal saline or that of fresh culture medium Immunization and monoclonal antibody production...
... than the level of HLA-DR in the cell lysates (5ul of a in 10 dilution, from x 106 PBMC) The shed HLA-DR therefore seemed to represent a small fraction ofthe total HLA-DR ofthe cell, however the ... respective surface expression Our results support the observations seen in the study of Fumeaux et al (2002) demonstrating that a modification of HLADR, post-translationally, may be one mechanism responsible ... expression in regulated in sepsis The demonstration that GM-CSF increased the shedding of HLA-DR from the cell surface and also increased HLA-DR gene transcription may have implications for the therapy...
... mgZn/kg based onthe uniform distribution of these metals in the sediments ofthe lake The higher concentration in the northwest part ofthe lake is probably due to reverse flow ofthe polluted Pasig ... untreated wastewater contained higher concentrations of lead and zinc The data onthemetal concentration of wastewater in the Philippines is not available In the case of other countries, Karvelas ... Accuracy ofthe measurement was confirmed through the analysis of Laguna Lake samples, as shown in the result section ofthe paper In addition, the accuracy was also confirmed through the monitoring...
... because these are the most distant pair of haems in the structure and are therefore expected to have the weakest interaction [33] The pH dependence ofthe chemical shifts ofthe NMR signals ofthe ... contribution to the reduction potential ofthe centres is small [40,41], and also with the experimental observation for monohaem cytochromes that the effect of anion binding or complex formation onthe reduction ... undisturbed by the replacement of Fe by Zn in the rubredoxin given the identical structures ofthe two protein forms [38,39] Therefore, at the concentrations used in the NMR experiments over 90% of the...
... to the iron(III) of Fe(ToCPP) and Fe(ToCPP))13G10 The second part of our results concerns thebindingof imidazole derivatives onthe iron of Fe(ToCPP) either alone in solution or inside thebinding ... (Table 1) Thebindingofthe ®rst CN± ligand to the iron was thus more easy in the hydrophobic binding pocket ofthe antibody than thebindingofthe second one, most probably because ofthe steric ... iron of Fe(porphyrin))13G10 complexes: (A) bindingof H2O2 onthe iron of 13G10-(Fe(ToCPP), (B) bindingof two CN± ligands onthe iron of 13G10-(Fe(ToCPP), (C) bindingof H2O2 and imidazole on...
... demonstrating the utility ofthe grafting of functional peptides with randomized edge sequences for optimizing the orientation ofthe grafted peptide on a scaffold protein In addition, when the ... the design of and screening for small binding proteins The preparation ofthe small binding proteins with binding function further allows us to increase thebinding strength by multibinding approaches, ... structural analyses and the construction of artificial libraries allow constructive selection and functionalization not only of antibody fragments, but also of small, nonantibody proteins (Fig...
... than the M3 in MtSTP Crystal contacts at the active site The differential occurrence ofthemetal ions correlates with the conformation ofthe flap subdomain (132–174) and thebindingof adjacent ... bindingofthe third metal ion suggesting, like Pullen et al [9], that rearrangement ofthe flap domain in HsSTP might lead to bindingof a third metal ion The implications ofthe M3 binding and ... adjacent STP monomers over the active sites of monomers C and D, but not of A and B (Fig 3A) For monomer C, which contains three metal ions, the monomer binding over the active site is monomer A...
... for the in situ immobilization and containment of contaminant metals and radionuclides in aqueous waste streams and subsurface environments, as some of these metals precipitate upon reduction ... reduction, as characterized by IC50s of 10.7 lm and 81.4 lm for inhibition of OmcA and OmcB, respectively Modes of inhibition of either OmcA or OmcB by V(V) The modes of inhibition of either OmcA- ... no upregulation or downregulation of FR (P ¼ 0.83) Onthe basis ofthe fact that FR is not subjected to regulation under the applied conditions, and deducing from Fig 2C that all other c-type...
... additional contacts with the molecule A 2164 Catalytic site The catalytic reaction of glucoamylases proceeds with inversion of configuration at the anomeric carbon which requires a pair of carboxylic ... addition revealed the presence of a second acarbose (substrate analogue) ˚ binding site onthesurfaceofthe enzyme, 25 A remote from the catalytic site The key residues involved in thebinding at ... glucoamylases demonstrate that mutations ofthe amino acids proposed to be involved in bindingofthesurface acarbose caused reduction of enzyme adsorption on starch, proving that these amino acids...
... different positions onthe fatty acid chain The uorescence yields depend onthe spinlabel position and the concentration ofthe labelled lipids The relation of these results to the depth ofthe uorophore ... same residue ofthe enzyme This conclusion was corroborated by the inhibition of PCD labeling in the presence of Na+ which resembles the effect of this coupling ion onthe reaction of cE65 with ... accessibility ofthebinding site from the aqueous environment The modication ofthebinding sites by DCCD was specically protected by Na+ that conrms the direct access of Na+ to the c subunit...
... respect to the role of HSP70 in the inhibition ofthe release of Smac and apoptosis GAPDH Ratio of HSP70 to GAPDH 14 # 12 # 10 * The role ofthe ATP -binding domain of HSP70 in the prevention ofthe ... cytotoxicity The protective effects of HSP70 were correlated with the level of HSP70 expression because the clone with higher HSP70 expression demonstrated a more significant reduction ofthe apoptotic ... Fig ATP -binding domain of HSP70 is essential for the inhibition of H2O2-induced activation of caspases-9 and -3 and apoptosis (A) The effects of HSP70 and its deletion mutant proteins onthe activation...