application of atomic force microscopy measurements on cardiovascular cells
Báo cáo hóa học: " Atomic force microscopy investigation of the kinetic growth mechanisms of sputtered nanostructured Au film on mica: towards a nanoscale morphology control" pot
... This amount corresponds to an average thickness of about nm This calculation gives a reasonable confirmation of the mass conservation law validity Concerning the formation of the small holes ... growth in two dimensions and considered the formation of depletion zones A depletion zone around a large cluster, originates from the shrunken smaller clusters Such depletion zones would have circular ... Furthermore, from the observation of the formation of depletion zones around the largest clusters and by the quantification of their time evolution, the Au surface diffusion coefficient Ds(T) = (7.42...
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Báo cáo hóa học: " Atomic Force Microscopy Study of the Kinetic Roughening in Nanostructured Gold Films on SiO2" ppt
... sputtered Au films are more consistent with a conservative deposition process (i.e prediction of the SP equation) rather than a nonconservative one (i.e prediction of the KPZ equation) Other experiments ... equation for nonequilibrium and conservative systems yields a = and b = 0.25 for growth of a two-dimensional surface [8] The values of a and b predicted by the theories for nonconservatives and conservatives ... the sample with a thickness of nm b of nm, c of 14 nm, d of 20 nm, e of 26 nm, f of 32 nm of Au respectively The continuous lines represent the fit by Eq The values of ci reported as insets are...
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Tài liệu Báo cáo khoa học: Dimers of light-harvesting complex 2 from Rhodobacter sphaeroides characterized in reconstituted 2D crystals with atomic force microscopy docx
... exist as an intermediate packing configuration Furthermore, we find that LH-2, in all cases, show a tilted conformation One of the two types of LH2 dimer configurations is shown to possess a tilt that ... dialysis [26] On the basis of AFM imaging of both the periplasmic and cytoplasmic sides of LH2’s after reconstitution, ellipticity and tilt of the LH2’s have been reported [14,21,23] A comparison between ... dimeric configuration Detailed investigations of the observed LH2 packing patterns reveal that the different packing lattices consist of similarly interacting LH2’s The dimeric LH2 configuration is...
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Báo cáo khoa học: The proximity between C-termini of dimeric vacuolar H+-pyrophosphatase determined using atomic force microscopy and a gold nanoparticle technique ppt
... GNPs on the C-termini of the enzyme In contrast, the longer distance indicates a probable extension of the C-termini of V-PPase Verication of these possibilities requires further investigation ... respect to the concentration of V-PPase and reaction time under these conditions (data not shown) After incubation, the reaction was terminated with a stop solution [1.7% (w/v) ammonium molybdate, ... cross-section shown in (B) Two populations of V-PPase protrusions were observed: one with a mean height of 1.2 0.1 nm (n = 20), and one with a mean height of 2.8 0.3 nm (n = 17) (D) Histogram of V-PPase...
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Báo cáo Y học: Structural heterogeneity of pyrimidine/purine-biased DNA sequence analyzed by atomic force microscopy potx
... (1995) Atomic- force microscopy of nucleoprotein complexes Scanning Microscopy 9, 705–727 Yang, J., Mou, J., Yuan, J.Y & Shao, Z (1996) The effect of deformation on the lateral resolution of atomic force ... sequences Conformational variability of triplex structures can provide tuning of the interaction of the triplexforming regions In addition, triplex formation absorbs the negative supercoils of the ... function in some processes of genome regulation such as replication rate and timing, recombination and chromosome folding by modulating the local structure of DNA regions and the global topology of...
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Báo cáo hóa học: "Nanopatterning on silicon surface using atomic force microscopy with diamond-like carbon (DLC)-coated Si probe" pptx
... scratched at 10 μN of tip force, μm/s of speed (c) The cross-section profiles of the grooves at the position as indicated by the line (d) Schematic of oblique cutting, inclination angle θ defined ... cross-section area increases much faster than the depth Consequently, the nonlinear behavior of the relationship between groove dimension and tip force results from the nonlinear increments of the ... National Natural Science Foundation of China (nos 10874040, 90306010, and 20803018) and the Cultivation Fund of the Key Scientific and Technical Innovation Project, Ministry of Education of China...
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báo cáo hóa học: " Comparison of immature and mature bone marrow-derived dendritic cells by atomic force microscopy" potx
... contaminated at the first touch and continue with the following touches, and this contamination can influence the next interaction of the tip with the cells Therefore, contamination control of ... causes nuclear translocation of the nuclear factor (NF)-kappaB transcription factor The inhibition of NF-kappaB activation blocks maturation of DCs, followed by down-regulation of major histocompatibility ... morphologic change is the foundation of the phenotype and the function of BMDCs MHC-II is one of activation molecules expressed on the surface of BMDCs, representing a phenotype of mature BMDCs Flow cytometry...
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Báo cáo hóa học: " Evaluation of the nanotube intrinsic resistance across the tip-carbon nanotube-metal substrate junction by Atomic Force Microscopy" pptx
... functionalization of CNTs with gold nanoparticles (AuNPs) is also investigated as a possible mean to improve their electrical conductivity Finally, the contiguous question of local modification of ... approach of the conduction mechanism with the diamond tip, we adapt our resistance model (see Figure 3b) by introducing the additional contribution of a Schottky diode between the R tip-CNT contact ... controlled by the Schottky barriers that forms the metal-CNT junction, the nature and geometry of this contact can strongly «Tip-CNT-substrate» junction analysis model The current conduction of...
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Báo cáo hóa học: " Optimizing atomic force microscopy for characterization of diamond-protein interfaces" docx
... on nano-interfaces of semiconductors and organic materials towards opto-electronic and bio-electronic applications Main interests lie in characterization and modification of material, electronic, ... wear rate of FBS layer on O-diamond using medium k cantilevers as a function of oscillation amplitude A in solution and in air Energy of oscillation at specific amplitude is given on the top ... possibility of tailoring diamond surface properties by various atomic terminations, it may have even broader application for fundamental studies Page of 10 of molecules on surfaces in general Based on...
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Báo cáo hóa học: " Conductive-probe atomic force microscopy characterization of silicon nanowire" pptx
... characterization of Schottky diodes based on boron doped homoepitaxial diamond films by conducting probe atomic force microscopy Superlatt Microstruct 2006, 40:343 21 Lampert A, Mark P: Current Injection ... explanations are that the whole surface of the remaining piece of silicon in contact with the a-Si:H layer fully contributes to decrease the electrical contact resistance or that the friction of the ... Conducting probe atomic force microscopy applied to organic conducting blends Appl Phys Lett 2001, 79:2993 19 Alvarez J, Kleider JP, Houze F, Liao MY, Koide Y: Local photoconductivity on diamond...
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Báo cáo hóa học: " Atomic Force Microscopy Study of Protein– Protein Interactions in the Cytochrome CYP11A1 (P450scc)-Containing Steroid Hydroxylase System" docx
... deviation Control of Functionality of Monomeric CYP11A1 These assays were aimed toward demonstrating the functionality of monomeric CYP11A1 For this purpose, we investigated the conversion of 7-dehydrocholesterol ... on particles in solution by use of photon correlation spectroscopy (PCS) Measurements were made at the light-scattering angle of 90° Protein solution (the stock one or the one subjected to monomerization ... height was built AFM of Non-Monomerized and Monomerized CYP11A1 AFM was used for visualization of non-monomerized and monomerized CYP11A1 The distribution densities r(h) of CYP11A1 images at...
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Báo cáo hóa học: "Scanned Probe Oxidation on p-GaAs(100) Surface with an Atomic Force Microscopy" doc
... corresponding pulse durations of 50 ms In the present work, the presented data are an average of five measurements To investigate the conversion mechanisms of GaAsoxides, the chemical composition of ... ratio of oxide nanostructures The proper control of the oxidation reaction, improvement of reproducibility and increasing the accuracy are among the immediate objectives for further evolution of ... within the context of the anodic oxidation concepts, the anionic and cationic transport are important factors in determining the kinetics of oxidation In this scenario, the driving force is the...
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báo cáo khoa học: "Atomic force microscopy: a powerful tool for high-resolution imaging of spermatozoa" ppt
... region c Power spectrum of the above profile showing the scaling of roughness d Histogram plot of the height of the head region e 3D image of the head region of the spermatozoa mechanical response ... region may often lead to the loss of functional competence of the spermatozoa The major advantage of AFM in pathological studies of spermatozoa is that it allows the evaluation of position and ... position of the viral particles located on the sperm membrane and their merging on the surface of spermatozoa were detected with high precision Unlike electron microscopy, and other conventional...
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báo cáo khoa học: "Immobilization of different biomolecules by atomic force microscopy" pptx
... shown) Consequently cross contaminations are negligible with this method Conclusions A novel approach has been presented for the high resolution immobilization of multiple biomolecules on a Page of ... contact mode with 3.4 mN contact force The tip remained in contact for seconds and changed to the next spotting positions by retraction Ink was supplied by a hypodermic needle of Popper & Sons, ... accuracy of spot positions, optimization and adaptation steps for deposition are reduced to a minimum, since only one chemical compound, neutravidin, is to be deposited Results and Discussion We...
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Atomic force microscopy study of malaria infected red blood cells
... presence of external force thermodynamic enthalpy thermodynamic entropy the force acting on the bond Boltzmann's constant dissociation constant equilibrium constant dissociation rate or off rate ... dissociation of the bond in vacuum the natural vibration frequency factor of the association of the bond in vacuum Free energy barrier width or mechanical reaction coordinate life time of molecular bond ... bond x List of Tables List of Tables Table 2-1 Summary of different surface modification and conjugation methods commonly used in AFM tip functionalization 45 Table 3-1 Comparison of knob phenotypes...
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Atomic force microscopy study of emodin treated MCF 7 human breast cancer cells
... illustration of the AFM indentation test on cells 20 Figure The effects of deformation force on AFM images of cell cortex 23 Figure The effects of scan direction on AFM images of living MCF-7 cells ... preparation 16 3.4 Atomic force microscopy 17 3.5 AFM imaging 17 3.6 AFM force curves 18 3.7 Young’s modulus calculation of the cells 19 CHAPTER AFM IMAGING STUDY OF MCF-7 CELLS 21 4.1 Introduction ... indentation on living MCF-7 cells 35 vii Figure 13 Force versus indentation curves for AFM indentation on living MCF-7 cells 37 Figure 14 The effects of emodin on the micromechanical properties of...
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atomic force microscopy, biomedical methods and applications
... Introduction One can easily distinguish between two general modes of operation of the atomic force microscope (AFM) depending on absence or presence in the instrumentation of an additional device ... (2001) Subatomic features in atomic force microscopy images Science 291, 2509 Jarvis, M R., Perez, R., and Payne, M C (2001) Can atomic force microscopy achieve atomic resolution in contact mode? ... samples: in fact, quite often, so-called atomic resolution” on crystals is obtained best with standard silicon nitride tips In general, one can choose among one of three types of tip The standard...
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Atomic Force Microscopy in Cell Biology_Methods in Cell Biology Volume 68 pptx
... Introduction Scanning Tunneling Microscopy Atomic Force Microscopy Force Spectroscopy Photonic Force Microscopy References 13 21 30 The Atomic Force Microscope in the Study of Membrane Fusion and ... Introduction Active Q Control Application of Active Q-Control AFM Transverse Dynamic Force Techniques Conclusions References 337 340 344 351 354 354 ix Contents 17 Scanning Force Microscopy Studies on ... Cell Adhesion Measured by Force Spectroscopy on Living Cells Martin Benoit I II III IV V Introduction Instrumentation Preparations of the Force Sensor for Measurements with Living Cells Cell...
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Atomic Force Microscopy: Biomedical Methods and Applications doc
... Introduction One can easily distinguish between two general modes of operation of the atomic force microscope (AFM) depending on absence or presence in the instrumentation of an additional device ... (2001) Subatomic features in atomic force microscopy images Science 291, 2509 Jarvis, M R., Perez, R., and Payne, M C (2001) Can atomic force microscopy achieve atomic resolution in contact mode? ... samples: in fact, quite often, so-called atomic resolution” on crystals is obtained best with standard silicon nitride tips In general, one can choose among one of three types of tip The standard...
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Atomic Force Microscopy in Cell Biology ppt
... Introduction Scanning Tunneling Microscopy Atomic Force Microscopy Force Spectroscopy Photonic Force Microscopy References 13 21 30 The Atomic Force Microscope in the Study of Membrane Fusion and ... Introduction Active Q Control Application of Active Q-Control AFM Transverse Dynamic Force Techniques Conclusions References 337 340 344 351 354 354 ix Contents 17 Scanning Force Microscopy Studies on ... Cell Adhesion Measured by Force Spectroscopy on Living Cells Martin Benoit I II III IV V Introduction Instrumentation Preparations of the Force Sensor for Measurements with Living Cells Cell...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008