a guide for the dental team
Báo cáo sinh học: " Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... http://www.virologyj.com/content/2/1/7 Acknowledgements We gratefully acknowledge the excellent technical assistance of Delia Barz and Jung-Won Sim-Bandenburg The authors are grateful to Andreas Kurth for critical reading of the ... out the SARS experiments WS participated in the design of the study AN carried out the CAMP experiments, participated in design and coordination of the study and helped to draft the manuscript All ... recommendations for oligo(dT)20 primed cDNA-synthesis cDNA synthesis was performed using µg of RNA, at 50°C Finally, cDNA was diluted 1:5 before use in QPCR Quantitative TaqMan PCR Primers, TaqMan...
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báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... http://www.virologyj.com/content/2/1/7 Acknowledgements We gratefully acknowledge the excellent technical assistance of Delia Barz and Jung-Won Sim-Bandenburg The authors are grateful to Andreas Kurth for critical reading of the ... out the SARS experiments WS participated in the design of the study AN carried out the CAMP experiments, participated in design and coordination of the study and helped to draft the manuscript All ... recommendations for oligo(dT)20 primed cDNA-synthesis cDNA synthesis was performed using µg of RNA, at 50°C Finally, cDNA was diluted 1:5 before use in QPCR Quantitative TaqMan PCR Primers, TaqMan...
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Báo cáo y học: " Phosphodiesterase type 4 expression and anti-proliferative effects in human pulmonary artery smooth muscle cells" docx
... (cAMP) represents a potential target as it is one of the main intracellular factors regulating cell proliferation and apoptosis [5] Prostacyclin analogues are an established vasodilator therapy ... therapy for PAH that act mainly via IP receptors to stimulate adenylyl cyclase and intracellular cAMP levels [6], but also have anti-proliferative actions on human PASMCs, which may be important for ... demonstrated that PDE4 genes are expressed in human distal PASMCs In addition to attenuating DNA synthesis and cell proliferation, stimulation of the cAMP signalling pathway was accompanied by increased...
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Báo cáo y học: " Ancient, independent evolution and distinct molecular features of the novel human T-lymphotropic virus type 4" pot
... TGACAGGGACAACGACCCTCTCCCAGGGGCGACAGCAAGCCCCCAAGGACAAAA c-Myb CTAGCAGGGACTAGTCATCAGCCAAAAAGGTCAACTGTCTCACACAAATAAGGAT bZIP910 Distal 21R in PTLV-1/2 CCGAAGGTTCTGACGTCCCAGCCCAGCCTCAAAACCAGGAAATCCATAGAAATGC ... CCGAAGGTTCTGACGTCCCAGCCCAGCCTCAAAACCAGGAAATCCATAGAAATGC Middle 21R ACCTCGCCCTTACCCACTTCCCCTATCATGAAAAACAAAGGCTGTGACGACTACCC Proximal 21R CCTTCCCCAAAAAATTTGCTTAAACCATCAATAAAGACAGCCTAGCCTATATAAGC TATA box poly (A) signal U3 R ATGAGGATGGTTCAGGAGGGGGCTCGCTCTCTTGCCGATCGCCCTGCTCACCTCGA ... 5'GGGGATGGTAAAGGCGAAGTAGGG3', and 1863LF3 5'CGTCCCAGCCCAGCCTCAAAACCA 3'and 1863PR5 5'GGGAATCTGGAAGAAAGCGTCCGT3', respectively The external and internal primer sequences for the pol-env fragment are...
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AC R Sample Task Type 4 Task
... Sample task type 4 Question 9 – 13 Complete the table below. Choose NO MORE THAN THREE WORDS from the passage for each answer. Write your answers in boxes 913 on your answer sheet. ... climate Complementary species Start of active period Number of generations per year French 2.5 cm cool Spanish late spring 1 2 Spanish 1.25 cm 9 10 11 South African ... 2.5 cm cool Spanish late spring 1 2 Spanish 1.25 cm 9 10 11 South African ball roller 12 13 ……… ...
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A comparative study of discourse structures and some major linguistic features of international declarations and international conventions on human rights part 4
... protect the child from all forms of sexual exploitation and sexual abuse For these purposes, States Parties shall in particular take all appropriate national, bilateral and multilateral measures ... pornographic performances and materials Article 35 States Parties shall take all appropriate national, bilateral and multilateral measures to prevent the abduction of, the sale of or traffic in ... financial assistance in case of need; (c) Make higher education accessible to all on the basis of capacity by every appropriate means; (d) Make educational and vocational information and guidance...
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Tài liệu E-Human Resource Management 4 pptx
... delivering as promised on time, quality, and price, then a mistake has been made in assessing their technical capacity; however, there has been no personal betrayal Affect-based trust on the other hand ... encouraged among managers within organizations As the organization becomes less hierarchical and structured, so the manager’s role changes As a consequence of having to trust people to perform to the ... 17 In addition to creating a learning organization, other HRM practices can also be useful to encourage a culture of innovation For example, reward and performance evaluation systems can be devised...
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Báo cáo khoa học: The Saccharomyces cerevisiae orthologue of the human protein phosphatase 4 core regulatory subunit R2 confers resistance to the anticancer drug cisplatin pot
... CATAGTGGAAAGAGGGATATAAATTATCGCATAAAACAATAAACAAAAAGAAAAATG AGGGAACAAAAGCTGGAG GGGAGTCAGCTGTTCGGTAACAGCATCTTGCATAGGCACATCTAAATCTGTATCCTGTAGGGCGAATTGGG GCAAAGTAAAACAGCACGAAAAAAGTGATTACAAATTTCAAGGGAGATATGATGAGGGAACAAAGGCTGGAG ... GGCAATTGGAGTGACATAGCAGCTACTACAACTACAAAAGCAAAATCTCCACAAAGTAAT CGGATCCCCGGGTTAATTAA CCAAGTGCTTCAATCCTAGAGAAGAAGAAAGGTAAGAAAAAGAAAGGAAAGCAACTTAAT GAATTCGAGCTCGTTTAAAC pFA 6a- HIS3MX6 pFA 6a- HIS3MX6 pFA 6a- 13Myc-HIS3MX6 ... GTAACTTCAGGTAGTAACTGGGCCTTGTATAGCCTTTCTAAACATTCGTCCAACTGTAGGGCGAATTGGG GAAATACTATTGAAGCTCAAAAACATCCATAATAAAAGGAACAATAACAATGGTAAGGGAACAAAAGCTGGAG GCGCCTGGCATTTCTTTATTGTTTCAAGCCATTCGTCGGGGCCTCTAGACTGTAGGGCGAATTGGG GGCAATTGGAGTGACATAGCAGCTACTACAACTACAAAAGCAAAATCTCCACAAAGTAAT...
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Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot
... R Alarcon et al Agmatinase activity of the N130D mutant arginase I Fig Effects of dialysis against EDTA on the catalytic activity of wild-type and N130D variants of human arginase I Fully active ... the other hand, upon dialysis 5628 against EDTA, the N130Q variant behaved exactly as described for the wild-type species The presumed conformational change accompanying the Asn130 fi Asp mutation ... positioned for optimal nucleophilic attack by the metal-bound hydroxide in the conformationally altered active site of the N130D variant On the other hand, as a potential ligand for a primary amino...
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Báo cáo khoa học: Effect of heliquinomycin on the activity of human minichromosome maintenance 4/6/7 helicase pdf
... mixture was incubated at 37 °C for 40 and then heated at 95 °C for Thereafter, bacterial alkaline phosphatase (0.6 units) was added, and the mixture was further incubated at 65 °C for 30 The mixture ... The radioactivity at the sites to which Pi and ATP migrated was measured, and the ratio of the released Pi to ATP was calculated The ratio in the case of the reaction performed without the enzyme ... Pi and ATP migrated was measured, and the ratio of the released Pi to ATP was calculated The ratio obtained in the case of the reaction performed without the enzymes was subtracted from that...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human UDP-galactose 4-epimerase pot
... to the generalized form than the peripheral form of disease Some of the mutants have only small changes in the kinetic parameters In the case of N34S and D103G, disease-causation may be explained ... nm to 330 nm for the mutants) The absorbance at 340 nm was measured for a further 7–8 Rates (v) were calculated by fitting the linear part of the A3 40 vs time plot to a straight line and corrected ... determinations Error bars represent the standard deviation of these mean values Table Kinetic constants of human UDP-galactose 4-epimerase (GALE) and the consequences of disease-causing mutations Values were...
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Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" pot
... Imaging Scandinavia, Nacka, Sweden) The amount of radioactivity in proteins was measured using a Molecular Imager FX, and the QuantityOne program from Bio-Rad Laboratories (Hercules, CA, USA) ... (v/v) acetic acid The gel was washed with water, dried and scanned by a CanoScan 8400F (Canon Svenska AB, Solna, Sweden) Western blot analyses The primary antibodies used were anti-gp60/110 (MAB8537) ... Inc., Santa Cruz, CA, USA) The secondary antibodies used were horseradish peroxidase conjugated donkey anti-rabbit IgG (NA934), sheep anti-mouse IgG (NA931) (Amersham Pharmacia Biotech, Uppsala,...
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Báo cáo sinh học: " The simultaneous presence and expression of human hepatitis C virus (HCV), human herpesvirus-6 (HHV-6), and human immunodeficiency virus-1 (HIV-1) in a single human T-cell" docx
... U16-17R gac act cca cca tag atc act c cat gat gca cgc tct acg aga c ctg tga gga act act gtc ttc acg cag cac tcg caa cca ccc tat cag cca gcn cac aaa ggn ata gga gg acb acy gcn cct tch cct ttc ccc aat ... of HIV1 and HCV in the same cell may greatly aggravate the malady Our TEM analysis showed HHV- 6A and HCV in the same cells, with extracellular HIV-1 particles budding or adjacent to the infected ... ccc ttt tct tta aaa tt cgt aga aca gaa gac cgg c aga act gca aat cgt tcc g [5] [5] [5] [5] [33] [33] [33] [34] [34] Page of (page number not for citation purposes) Virology Journal 2007, 4:106...
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Báo cáo sinh học: " Thiol-reactive reagents inhibits intracellular trafficking of human papillomavirus type 16 pseudovirions by binding to cysteine residues of major capsid protein L" pot
... 5'-CTTGCAGATGCTGGTGGC GATGTCCAGGGGCAC-3' (R for C22 5A) , 5'-ACCAGCATCGCCAAGTACCCCGACTACATC-3' (F for C22 9A) , 5'-ATGTAGTCGGGGTACTTGGCGATGCTGGTGCAGAT G-3' (R for C22 9A) , 5'-CAACAACGGCATCGCCTGGGGC AACCAGCTGTTC-3' ... C22 5A, C22 9A 5'-AACCTGGCCAGCAGCAACTACTTCCC-3' and 5'-AACTAGAAGGCACAGTCGAGGCTG-3' were used similarly to produce C32 4A, C34 5A, C37 9A The resultant DNA fragments were inserted into ph16L1 after digestion ... CCGATCAGGCACAG-3' (R for C16 1A) , 5'-AACCCCGGCGACGCCCCCCCCCTGGAGCTG-3' (F for C18 5A) , 5'CAGCTCCAGGGGGGGGGCGTCGCCGGGGTTC-3' (R for C18 5A) , 5'-GTGCCCCTGGACATCGCCACCAGCATC TGCAAG-3' (F for C22 5A) ,...
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Báo cáo sinh học: " The E5 protein of the human papillomavirus type 16 down-regulates HLA-I surface expression in calnexin-expressing but not in calnexin-deficient c" pptx
... presented above, and together with the fact that HLA-I and calnexin associate during HLA maturation, we hypothesized that the formation of a trimeric complex between HLA-I heavy chain, calnexin and ... the manuscript FM performed the peptide translocation assay AA conceived and supervised the study and drafted the manuscript PT collaborated in the supervision of the study and helped draft the ... IP: anti-calnexin anti-calnexin anti-E5-tag (AU1) pCI E5 100 anti-E5-tag (AU1) pCDNA E5 100 IP: anti-E5-tag (AU1) anti-calnexin 10 C 100µg anti-calnexin anti-E5-tag (HA) 10 E pCI 100 10 IP: anti-E5-tag...
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Báo cáo sinh học: " Human herpesvirus 6A induces apoptosis of primary human fetal astrocytes via both caspase-dependent and -independent pathways" potx
... was associated with activation of caspase-3 and cleavage of poly (ADP-ribose) polymerase (PARP), which is known to be an important substrate for activated caspase-3 Caspase-8 and -9 were also ... proteases, regulate the initiation and the final execution of apoptosis in receptor-mediated and mitochondria-mediated pathways [12] In the receptor-mediated pathway, caspase-8 is the initiator caspase ... found that PARP was cleaved in HHV- 6A- induced apoptotic PHFAs Caspase-3 is a common effector of both death receptor and the mitochondrial signaling pathways Caspase-8 is activated by the death receptor...
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Báo cáo sinh học: " Human herpesvirus 8 – A novel human pathogen" potx
... Israel M, Liotta L: The Molecular Basis of Cancer Philadelphia, PA: WB Saunders Company; 1995 Kawano M, Hirano T, Matsuda T, Taga T, Horii Y, Iwato K, Asaoku H, Tang B, Tanabe O, Tanaka H, et al.: ... in the US AIDS KS samples, but the B variant was predominant in samples from Africa C variants were observed from samples from Saudi Arabia and Scandinavia The D subtype was uncommon and was ... 51(11):845-852 199 Ayuthaya PI, Katano H, Inagi R, Auwanit W, Sata T, Kurata T, Yamanishi K: The seroprevalence of human herpesvirus infection in the Thai population Southeast Asian Journal of Tropical Medicine...
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nghiên cứu phát hiện sự gắn chèn gene e6 và e7 của human papilomavirus type 16 vào bộ gene người bằng kỹ thuật multiplex rt-pcr
... AGG NNNNCNNGNANATNANCACACAACGGTTTGTT GTATTGCTGTTCNAATGTTGTTCCATACAAACT ATAACAATAATGTCTATACTCACTAATTTTAGA ATAAAACTTTAAACATTTATCACATACAGCATA TGGATTCCCATCTCTATATACTATGCATAAATC CCGAAAAGCAAAGTCATATACCTCACGTCGCA ... ACAGTTACTGCGACGTGAGGTATATGACTTTGC TTTTCGGGATTTATGCATAGTATATAGAGATGG GAATCCATATGCTGTATGTGATAAATGTTTAAA GTTTTATTCTAAAATTAGTGAGTATAGACATTAT TGTTATAGTTTGTATGGAACAACATTAGAACAG CAATACAACAAACCGTTGTGTGATTTGTTAATT ... CTATTAGCGGAGGCTCCAACGAGGGGTCCACGGACACCACCTCC ACTCACACAACCAACACTCAGAACAATGACTGGTTTTCAAAGCT GACCAGTTCCGCTTTTAGCGGTCTTTTCGGCGCTCTTCTTGCTGAC AAGAAAACCGAGGAGACCACTCTTCTCGAGGACCGCATCCTCAC TACCCGCAACGGACACACGACCTCGACAACCCAGTCGAGCGTTG...
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Nuclear Power Control, Reliability and Human Factors Part 4 docx
... development in Japan, USA, Canada, China, and the EU Robots, as “environmental” guards, have some advantages and disadvantages From one side, having limited “intellectual” abilities, a robot cannot find ... signal validation adds information about data quality For communications, the functional elements include device-level data and control signals, An Approach to Autonomous Control for Space Nuclear ... information and commands, and spacecraft-level status and demands The effective integration of data and information at each level requires a well-defined functional architecture with a capable...
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Báo cáo hóa học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" potx
... Imaging Scandinavia, Nacka, Sweden) The amount of radioactivity in proteins was measured using a Molecular Imager FX, and the QuantityOne program from Bio-Rad Laboratories (Hercules, CA, USA) ... (v/v) acetic acid The gel was washed with water, dried and scanned by a CanoScan 8400F (Canon Svenska AB, Solna, Sweden) Western blot analyses The primary antibodies used were anti-gp60/110 (MAB8537) ... Inc., Santa Cruz, CA, USA) The secondary antibodies used were horseradish peroxidase conjugated donkey anti-rabbit IgG (NA934), sheep anti-mouse IgG (NA931) (Amersham Pharmacia Biotech, Uppsala,...
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