... 1. 1.4 Subtypes of PPARs 1. 1.5 Ligands and physiological functions of PPARα and PPARδ 1. 1.6 Ligands of PPARγ 1. 1.7 PPARγ and adipogenesis 10 1. 1.8 PPARγ and ... 12 1. 1.9 PPARs and cancer 13 1.1 .10 PPARγ and breast cancer 15 1. 2 ESTROGEN RECEPTORS (ERS) 18 1. 2 .1 Identification and structures of ERs 18 1. 2.2 Mechanism ... 34 1. 3.5 NHE1 and cell migration 35 1. 3.6 NHE1 and heart disease 35 1. 3.7 NHE1 and cancer 36 1. 3.8 Regulation of NHE1 activity 39 1. 3.9 Regulation of NHE1...
... in NHE1 mRNA by NAC (Figure 11 C), further confirming that ROS/RNS 10 5 contributes approximately 20% of the overall down-regulation of NHE1 transcription by 5M of 15 d-PGJ2 10 6 Figure 11 : ROS/RNS ... PPARγ ligands: (A) 15 d-PGJ2, (B) troglitazone for 24h, and the protein expression of NHE1 was analyzed by Western blot (C) MDA-MB-2 31 (2X105 cells/6-well dishes), and T47D cells (3 X105 cells/6-well ... X 10 6 /10 0mm dish) and (B) MDA-MB-2 31 (1. 7 X 10 6 cells /10 0mm dish) cells were exposed to 3M 15 d-PGJ2 for 4h before harvesting for nuclear-cytosol fractionation as described in Materials and Methods...
... alpha 13 1 Figure 19 : Re-expression of ERα blocks PPARγ-mediated down-regulation of NHE1 expression in MDA-MB-2 31 cells (A) MDA-MB-2 31 (2X105 cells/6-well dishes), and (B) MDA-MB-2 31 ERα+ (2X10 cells/6-well ... (2X10 cells/6-well dishes) and MDA-MB-2 31 ERα+ (2X105 cells/6-well dishes) cells were treated with 15 d-PGJ2 for 16 h with or without 2h pre-incubation of 1 M E2, and the fold change of NHE1 mRNA ... with 15 d-PGJ2 for 24h with or without 2h pre-incubation of 1 M E2, and the protein expression of NHE1 was 13 2 analyzed by Western blot NHE1 band intensity was normalized to β-actin (C) MDA-MB-231...
... Diabetologia 51( 11) : 210 8 211 6 Jurka, J and A Milosavljevic (19 91) "Reconstruction and analysis of human Alu genes." J Mol Evol 32(2): 10 5 -12 1 Kallinowski, F., P Vaupel, et al (19 88) "Glucose ... Symp Proc (1) : 14 9 -16 1 Hartman, J., K Lindberg, et al (2006) "Estrogen receptor beta inhibits angiogenesis and growth of T47D breast cancer xenografts." Cancer Res 66(23): 11 207 -11 213 Hauser, ... 14 64 -14 76 Badawi, A F and M Z Badr (2003) "Expression of cyclooxygenase-2 and peroxisome proliferator-activated receptor-gamma and levels of prostaglandin E2 and 15 deoxy-delta12 ,14 -prostaglandin...
... MDA-MB-2 31 (2 X 10 5 cells/6-well dishes) and T47D (3 X105 cells/6-well dishes) cells were treated with 15 d-PGJ2, for 16 h before they were washed and reseeded at a density of 1. 0 X 10 4 cells /10 0mm ... MCF-7 (3 X10 cells/6-well dishes) cells were exposed to (A) 15 d-PGJ2 and (B) ciglitazone for 16 h before they were washed and reseeded at a density of 1. 0 X104 cells /10 0mm dish After 14 days of ... of 15 d-PGJ2 treatment for 16 h before the colony forming assay was performed as described in Materials and Methods Similar to what we observed in MCF-7, 15 d-PGJ2 resulted in a dose-dependent 11 1...
... regular serum were given 10 nM fulvestrant or 10 0nM raloxifene for 2h before the introduction of 15 d-PGJ2 The cells were trypsinized and reseeded onto 10 0mm dish after 18 h, and a 14 -day colony forming ... with 15 d-PGJ2 for 48h with or without 2h pre-incubation of 10 nM Fulvestrant in medium containing 10 % regular serum, and the protein expressions of NHE1 was analyzed by Western blot NHE1 band intensity ... (C) MDA-MB-2 31 ERα+ (1. 5 X10 cells/6-well dishes) cells were treated with 15 d-PGJ2 for 40h with or without 2h pre-incubation of 10 nM Fulvestrant in media containing 10 % regular serum, and the fold...
... 2, pUC19 and 10 mM DTT; lane 3, pUC19 and lM FeCl3; lane 4, pUC19, 10 mM DTT, and lM FeCl3; lane 5, pUC19, 10 mM DTT, lM FeCl3, and 50 lgÆmL )1 rBcp2; lane 6, pUC19, 10 mM DTT, lM FeCl3, and 50 ... thioredoxin reductase and thioredoxin) (Fig 1) Two archaeal Prxs from Aeropyrum pernix APE2278 [10 ] and Pyrococcus horikoshii PH1 217 [11 ,12 ] have A H2O 1- Cys Prx 1- Cys Prx H2O2 Sp OH 722 Identification ... solfataricus P2 [14 ] (http://www-archbac.u-psud.fr/ projects/sulfolobus/) revealed four ORFs homologues of Prxs and annotated as Bcp1 (SSO20 71) , Bcp2 (SSO 212 1), Bcp3 (SSO 225) and Bcp4 (SSO2 613 ) The S...
... two subunits in calpains [18 ] Indeed, the two subunits of 80 kDa (domains I–IV) and 30 kDa (domains V–VI), contain altogether 10 11 putative EF-hands motifs in domains IV, VI and II [1, 19,20], ... calcium (Figs 2A and 5B, 10 min) as in its presence (not shown) Furthermore, the 76, 50 and 30 kDa fragments issued from the 12 0 autolysis of Calp1, and recognized by anti-Calp1 (Fig 5Ba, 12 0 min), ... (19 97) Calpains: intact and active? Bioessays 19 , 10 11 10 18 Rutledge, T.W & Whiteheart, S.W (2002) SNAP-23 is a target for calpain cleavage in activated platelets J Biol Chem 277, 37009–37 015 ...
... accident: the combination of sharp increases in interest rates from late 19 79 until 19 81 and a severe recession in 19 81 19 82, both of which were engineered by the Federal Reserve to bring down ... Losses for savings and loan institutions mounted to $10 billion in 19 81 19 82, and by some estimates more than half of the S&Ls in the United States had a negative net worth and were thus insolvent ... legislation in 19 80 and 19 82 that made it easier for savings and loans to engage FYI The Principal-Agent Problem in Action: Charles Keating and the Lincoln Savings and Loan Scandal As we have...