Characterization of liver x receptor and retinoid acid receptor mediated response with transcriptome and histological analysis in zebrafish liver
Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modifications and activity-based analyses doc
... Gevaert et al COFRADIC and protein modifications analysis of purine-binding proteins in a total lysate of human Jurkat T-cells [40] primary separation mAU 1400 COFRADIC analysis of protein processing ... [34,36] and N-glycosylation [39] – and describe the use of COFRADIC for studying interactions between small molecules and proteins The latter is a particular application of ‘post-translational COFRADIC’, ... COFRADIC and protein modifications K Gevaert et al large number of species, and it now suffices to generate partial protein sequence information with which to access entire (predicted) protein...
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Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx
... to the 9th cycle from the N-terminus including the initiating Met: MHKTHSTMS for Sno1p and MTGEDFKIKS for Snz1p Properties of the complex of Sno1p and Snz1p When Sno1p with a His-tag and Snz1p ... SNO3, SNZ2 and SNZ3 complement the defect The relationship of all of these genes remains to be clarified Expression and purification of Sno1p and Snz1p In light of the similarity in the amino acid ... Sequencing of the mutated genes revealed that there was indeed a mutation on both of the genes In SNO1, the 199th G from the 5¢ terminus of the open reading frame was deleted to result in a frame-shift...
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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx
... blue, hematine, and p-chloranil were obtained from ICN; hydroxyethyl disulfide (HEDS) and vinylene trithiocarbonate was obtained from Aldrich EST identification and cDNA isolation The S mansoni EST ... (lane 4) electroblotted and immunodetected by a-SmGSTO serum (B) RT-PCR analysis of different S mansoni stages Reverse-transcribed RNA from miracidia (lane 1), sporocysts (lane 2), cercariae (lane ... growth of murine macrophages and fibroblasts and cytokine gene expression Immunol Lett 78, 127–134 O’Leary, K.A & Tracy, J.W (1988) Purification of three cytosolic glutathione S-transferases from...
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Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
... mansoni NR1 A B W Wu et al DNA binding domain Ligand binding domain Fig Sequence alignment (A) Alignment of DNA binding domain (C domain) and its C-terminal extension (B) Alignment of ligand binding ... assay in in vivo results Experimental procedures Parasites The NMRI strain of S mansoni was maintained in snails (Biomphalaria glabrata) and Syrian golden hamsters (Mesocricetus auratus) Cercariae ... conserved, the P-box (EGCKG), which is involved in determining DNA binding specificity, is identical to most members of nuclear receptor subfamily I, for instance retinoic acid receptor (RAR) and vitamin...
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Báo cáo khoa học: Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and flux control analysis ppt
... objective of understanding how the glycolytic ux in E histolytica is controlled, we cloned the genes, and overexpressed, puried and determined the kinetic parameters of the 10 glycolytic enzymes ... reconstruction of the nal stages of amoebal glycolysis Analysis of the kinetic properties of the recombinant glycolytic enzymes indicated that EhPPDK and EhPGAM had the slowest activity and were the ... Overexpression and purication of recombinant enzymes One-hundred millilitres of LB (LuriaBertani) [59] containing 30 lgặmL of kanamycin (for EhPPDK 100 lgặmL of ampicillin [11]) was inoculated with...
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Báo cáo Y học: Baboon cytochrome P450 17a-hydroxylase/17,20-lyase (CYP17) Characterization of the adrenal microsomal and cloned enzymes docx
... 16a-hydroxyprogesterone Both expressed enzymes had no lyase activity towards PROG or 17-OHPROG Understanding of the complexity of hydroxylase and lyase activity of CYP17 by way of primary sequence ... the metabolism of PREG by the two enzyme preparations Despite the large degree of homology between the human and baboon CYP17 the expressed baboon CYP17 was unable to catalyse the formation of ... CYP17 in the catalytic activity, making the baboon an important CYP17 candidate for the study of structure/function relationships CYP17 is a membrane-bound microsomal cytochrome P450 In the adrenal...
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Báo cáo khoa học: Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola A carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle ppt
... purification of the recombinant enzyme In order to construct an expression vector for Cl-idh, two oligonucleotides (sense, 5¢-AAAAACATATGGCAAGCA AATCGACCATCATCTACAC-3¢, and antisense, 5¢-AAA AAGGATCCCGGCTGAAAACCGGGCTGCATTA-3¢) ... dehydrogenase is known to predominantly catalyze the reduction of oxaloacetate to malate, and thereby lowering the possibilities of oxaloacetate accumulation Indeed, when we have analyzed the malate dehydrogenase ... during the decarboxylation of isocitrate, and indeed, the site-specific mutagenesis of K253 to Met led to an inactive protein [23] In addition, the alkylation of the adjacent M258 inactivated the...
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Báo cáo khoa học: The HS:19 serostrain of Campylobacter jejuni has a hyaluronic acid-type capsular polysaccharide with a nonstoichiometric sorbose branch and O-methyl phosphoramidate group docx
... structure for the HS:19 serostrain and have shown that these labile groups are an a- l-sorbofuranose branch attached at C2 of b-d-GlcA and a MeOPN modification located at C4 of b-d-GlcNAc There are very ... the disappearance of signals associated with the sorbose monosaccharide and the production of the same oxidation product as observed for the l -sorbose standard (Figs 5E,F) On the basis of these ... a- L-sorbofuranose branch located at C2 of GlcA and an MeOPN group at C4 of GlcNAc Structural heterogeneity is due to the nonstoichiometric sorbofuranose branch and the variably methylated nonstoichiometric...
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Báo cáo khoa học: Characterization of rat cathepsin E and mutants with changed active-site residues and lacking propeptides and N-glycosylation, expressed in human embryonic kidney 293T cells ppt
... haemagglutinin at the C-terminus and encoding the cathepsin D propeptide fused to mature cathepsin E, we have demonstrated that the propeptide of cathepsin E, likewise those of other aspartic proteinases, ... maturation of cathepsin E, we constructed a mutant lacking the propeptide (Leu23-Phe58) and expressed it in HEK -293T cells The transfected cells were pulse-labeled with [35S]methionine for 30 and ... Pulse–chase analysis of wild-type cathepsin E expressed in HEK -293T cells (A) The transfected cells were metabolically labeled with [35S]methionine ⁄ cysteine for 30 and chased for the times indicated...
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Báo cáo khoa học: Characterization of a Cry1Ac-receptor alkaline phosphatase in susceptible and resistant Heliothis virescens larvae potx
... kept at °C and used within 10 days Quantification of alkaline phosphatase and aminopeptidase activities Specific alkaline phosphatase (ALP) and N-aminopeptidase (APN) enzymatic activities of BBMV ... sugar Canavalis ensiformis (ConA) a- Man a- Glc Galb1 fi 3GalNAc Galb1 fi 3,4GlcNAc a/ bGalNAc a/ bGal Galb1 fi 4GlcNAc Gala1 fi 3Gal GalNAca1 fi 3GalNAc GalNAca1 fi 3Gal Galb1 fi 3GalNAc Galb1 fi 3,4GlcNAc ... amounts in BBMV from YHD2 larvae would result in reduced alkaline phosphatase activity Alkaline phosphatase activity in blots of BBMV proteins from YDK and F1 larvae was similar, and higher than activity...
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Báo cáo khoa học: Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins pptx
... We interpret this observation as the retainment of newly synthesized proteins in the cytosol due to the inability to import matrix proteins by the fraction of growing and dividing glycosomes The ... lower (not shown) In order to determine the in uence of the reduction of the expression of TbPEX14 on the import of glycosomal matrix proteins, the subcellular distribution of glycolytic enzymes ... identified in yeast) PEX17 Several other peroxins are involved in the subsequent steps of the import The import of matrix proteins seems to involve a cascade of interactions between the cargo-loaded...
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university of calgary press space and spatial analysis in archaeology dec 2005
... space and spatial reckoning are inextricably culture- (and language-) bound, and a better understanding of space and the experience of living in space should be one of the primary goals of the ... Settlement and Urbanism: Proceedings of a Meeting of the Research Seminar in Archaeology and Related Subjects Held at the Institute of Archaeology, London University Research Seminar in Archaeology and ... of Space (Hillier and Hanson 1984) has inherently Durkheimian underpinnings by conceiving of the organization of space in terms of organic and mechanical solidarity In archaeology, the work of...
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Báo cáo toán học: "A Hybrid of Darboux’s Method and Singularity Analysis in Combinatorial Asymptotics" potx
... Plan of the paper We start by recalling in Section the principles of two classical methods dedicated to coefficient extraction in combinatorial generating functions, namely Darboux’s method and singularity ... at the singularity z = can be determined, resulting in (7) (The representation in (7) is in fact exact and not merely asymptotic It has been obtained by Zagier and Cohen in [27, p 387], and is ... singular part of h(z) (up to O t terms) A basic result of singularity analysis theory enables us to extract coefficients of functions that admit of such expansions Lemma (Singularity analysis method) ...
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Characterization of liver x receptor and retinoid acid receptor mediated response with transcriptome and histological analysis in zebrafish liver
... CHARACTERIZATION OF LIVER X RECEPTOR- AND RETINOID ACID RECEPTOR- MEDIATED RESPONSE WITH TRANSCRIPTOME AND HISTOLOGICAL ANALYSIS IN ZEBRAFISH LIVER HENDRIAN SUKARDI NATIONAL UNIVERSITY OF SINGAPORE ... Transcriptomic response to liver X receptor (LXR) agonist T0901317 in zebrafish liver 28 3.1 29 3.2 3.3 3.4 Histological analysis of T0901317-induced effects and toxicity in zebrafish liver Microarray experiment ... 24 2.5 Histological processing and analysis 2.5.1 Histological processing, sectioning, and hematoxylin and eosin staining For the histological processing, adult zebrafish were treated with different...
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Từ khóa: Báo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt nam