Analysis of gonad differentiation in zebrafish by histology and transgenics

... ANALYSIS OF GONAD DIFFERENTIATION IN ZEBRAFISH BY HISTOLOGY AND TRANSGENICS WANG XINGANG (B Sc., Ocean University of Qingdao, China) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... be on discussing the mode of sex determination and candidate genes involved in gonad differentiation of zebrafish 1.2 Sex determination mechanism of some invertebrates (fruit fly and worm) The ... absence of a sex linked marker zebrafish cannot be sexed, which hinders the studies of molecular mechanism of gonadal differentiation Traditional method using Hematoxylin and Eosin staining requires...

Báo cáo vật lý: "Analysis of Trace Elements in Teeth by ICP-MS: Implications for Caries" pptx

... concentrations of trace elements found in the pulps of the teeth Trace Elements in Teeth: Implication for Caries Table 5: Concentration of trace elements (ppm) in permanent healthy and carious teeth ... Trace Elements in Teeth: Implication for Caries teeth has been suggested as being indicative of Pb toxicity.5,9 Here, we have investigated the importance of the location of trace elements in ... habits influence the accumulation of trace elements in the teeth For this purpose, they determined the concentrations of different elements in the enamel, dentine crown, dentine root and the pulp in...

FUNCTIONAL GENOMIC ANALYSIS OF GONAD DEVELOPMENT IN THE PROTANDROUS ASIAN SEABASS

... activation of the ‘profemale’ Wnt signaling pathway In order to understand the role of the brain in the sex VIII change process, a microarray analysis was also carried out on the brain of adult ... on the gonadal maturation stage Long-term treatment of GnRH could also promote the development of spermiating testis in juvenile seabass To test the hypothesis on the role of Wnt signaling in ... level in the zebrafish testis than in the ovary (von Hofsten and Olsson 2005, Sreenivasan et al 2008) According to the classical theory, the differentiation of the ovary in the absence of the male...

A PRAGMATIC CULTURAL ANALYSIS OF COHESIVE DEVICES IN SPEECHES BY BARRACK OBAMA AND MITT ROMNEY IN UNITED STATES PRESIDENTIAL ELECTION 2012

... DEBATE BY BARACK OBAMA AND MITT ROMNEY IN UNITED STATES PRESIENTIAL ELECTION, 2012 This chapter deals with the use of cohesive devices in the third debate by Barack Obama and Mitt Romney in Unites ... STATES PRESIDENTIAL ELECTION, 2012 AND “THE THIRD PRESIDENTIAL DEBATE” 16 CHAPTER 3: AN ANALYSIS OF COHESIVE DEVICES IN THE THIRD DEBATE BY BARACK OBAMA AND MITT ROMNEY IN UNITED STATES ... cohesion of a discourse in a particular context and detailed analysis of cohesive devices used in the third debate by Barack Obama and Mitt Romney in Unites States presidential election, 2012 Therefore,...

Báo cáo y học: "Systematic analysis of gene expression in human brains before and after death" docx

... any prolonged agonal conditions, however, death itself may alter gene expression patterns in postmortem human brains Study of expression levels of 14 genes in human brain autopsy and biopsy samples ... significantly increased after death, while the expression of genes involved in protein biosynthesis, rRNA processing, organelle organization and biogenesis and induction of apoptosis are significantly ... found in the categories 'organelle organization and biogenesis' and 'intra- cellular protein transport' (Table 2) The expression of genes involved in the ubiquitin cycle and protein ubiquitination...

Molecular analyses of gonad differentiation and function in zebrafish

... help in the study of gonad differentiation in zebrafish Studying gene function utilizing widely used molecular tools, such as gene knock-out, morpholinos, and cell culture, is also difficult in zebrafish ... during gonad differentiation: foxl2, cyp19a1a, cyp11a, hsd3b and cyp17a1 in ovarian differentiation and star, nr5a1a and cyp11b2 in testicular differentiation In order to broaden the understanding ... examine the gonad development 17 To explore the dynamic process of gonad differentiation, three transgenic zebrafish lines expressing EGFP in their gonads have been generated In one of these lines,...

An error analysis of using reference in written english by secondary school studend = phân tích lỗi của học sinh THPT tong việc sử dụng phép quy chiếu trong tiếng anh viết

... capability of using language Finding and analyzing errors by learners; therefore, are significant to improve learning and teaching quality Error analysis (EA) has been defined by many linguists in different ... knowledge of reference as well as good ability to use reference as follows: An analysis of errors made by secondary- students in using personal reference in English An analysis of errors made by secondary- students ... cataphoric reference is much more limited than that of anaphoric reference - The major causes of errors in using English reference are mother tongue interference (interlingual errors), intralingual errors,...

báo cáo sinh học:" Meeting human resources for health staffing goals by 2018: a quantitative analysis of policy options in Zambia" potx

... component of voluntary attrition [26] Training parameters Training parameter estimates were derived from a 2008 joint Ministry of Health and Clinton Foundation assessment of all 39 medical training institutions ... Expanding health training institutions in Zambia: operational scale-up plans through individual school assessment, in Human Resources for Health Results Addis Ababa, Ethiopia; 2009 28 Musonda ... currently a surplus of qualified applicants at the national training institutions, this may change if training institution enrolment were increased dramatically Adequate preparation in primary and...

Báo cáo y học: "Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad-range PCR, cloning and sequencing" pps

... using broad-range PCR and/ or reverse transcription PCR systems to search for bacterial DNA and RNA in synovial samples from patients with various forms of arthritis, including ReA [12,14,17] By ... bacterial DNA in synovial samples We extracted DNA from ST samples from 28 patients with arthritis We found bacterial DNA in 21 (75%) of these patients, using stringent sterility and anti-contamination ... studies, using PCR assays with universal 16S rDNA primers, identified lower proportions of human synovial samples containing bacterial DNA: 42% of synovial fluid and ST samples in one study [18], and...

báo cáo khoa học: "Quantitative analysis of nanoparticle internalization in mammalian cells by high resolution X-ray microscopy" pps

... this article as: Chen et al.: Quantitative analysis of nanoparticle internalization in mammalian cells by high resolution X-ray microscopy Journal of Nanobiotechnology 2011 9:14 Submit your next ... uptake microanalysis by EMT cells after co-culturing with 500 μM naked AuNPs for 48 h The size distribution in A) was obtained by analyzing individual cells such as that in B) Bars in B: 10 μm ... required staining Figure shows that even without staining this imaging method can readily explore in detail the dissimilarities in the internalization of different AuNPs by different cell lines Specifically,...

Báo cáo y học: " Identification of structural aberrations in cancer by SNP array analysis Stefan Heinrichs and A Thomas Loo" pot

... information provided by SNP arrays To uncover novel signaling pathways in human cancers, they first examined the structural genomic aberrations of a cell line panel by SNP array copy-number analysis ... significance curve and clustering of SNP- array- based loss -of- heterozygosity data Bioinformatics 2004, 20:1233-1240 26 dChip Software: Analysis and Visualization of Gene Expression and SNP Microarrays ... Biology 2007, Volume 8, Issue 7, Article 219 Heinrichs and Look Advantages of SNP array analysis A SNP is defined as a DNA sequence variation at one specific position in the genome that occurs in at...

Generation and analysis of KRAS v12 in driving liver tumorigenesis using transgenic zebrafish models

... Results….….….…….….….…….….….…….….….…….….….…….46 3.1 Analysis of constitutive liver- specific expression of oncogenic krasV12 in driving liver tumorigenesis in transgenic zebrafish .………… 47 3.1.1 Generation of Tg(fabp10:EGFP-krasV12) transgenic ... different sets of experiments were designed and performed  Generation and analysis of constitutive liver- specific expression of oncogenic krasV12 in driving liver tumorigenesis in transgenic zebrafish ... generation and analysis of zebrafish models of human diseases (Amatruda and Patton, 2008; Liu and Leach, 2011) Owing to the ease of housing maintenance, short generation time and fecundity, zebrafish...

Microarray analysis of host transcription in response to infection of arabidopsis thaliana protoplasts by coat protein mutants of TCV and HCRSV

... MICROARRAY ANALYSIS OF HOST TRANSCRIPTION IN RESPONSE TO INFECTION OF ARABIDOPSIS THALIANA PROTOPLASTS BY COAT PROTEIN MUTANTS OF TCV AND HCRSV LUO QIONG (M Sc., CAAS) ... virus mutants: HCRSV- CPTCV and TCV- CPHCRSV To transfect A thaliana and H cannabinus protoplasts with TCV, HCRSV, TCV- CPHCRSV and HCRSV- CPTCV and to detect their replication and CP expression To investigate ... TCV- CPHCRSV, HCRSV and HCRSV- CPTCV could replicate in Arabidopsis protoplasts Figure 5.2 TCV, HCRSV and HCRSV- CPTCV could replicate in Hibiscus protoplasts Figure 5.3 TCV, TCV- CPHCRSV, HCRSV and HCRSV- CPTCV...

Analysis of Phosphorus Behavior in the Giant Reed for Phytoremediation and the Biomass Production System

... the biomass of the giant reed and maintain the wetland potential is proposed as shown in Fig 10 In this method, the above-ground part of the giant reeds that will remain the following year are ... rhizomes remaining for the next year Thereafter, the giant reeds grow with the rhizome extension, and the giant reed community in the wetland is regenerated - 152 - Journal of Water and Environment ... accumulation, and translocation properties of the giant reed And based on the property, we propose a management method of a water treatment system using the giant reed Fig - Appearances of the Giant Reed, ...

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Energy efficiency and cost analysis of canola production in different farm sizes

... production in different farm sizes in terms of energy use efficiency and economical analysis They reported that, both the total energy input and output energy in apricot production decreased when farm ... the main objective of this study was to compare the energy use and economic efficiencies of canola production in different farm sizes in Golestan province of Iran Materials and methods The investigation ... using the Microsoft Excel spreadsheet and SPSS 17.0 software programs Results and discussion 3.1 Analysis of input-output energy use in canola production The amount of inputs and outputs for canola...

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