báo cáo khoa học: " Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leaves" pps

... 10RESEARC H ARTIC LE Open Access Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leavesLi-Jun Wang1, Ling Fan1,2, Wayne Loescher3, ... pro-tein aggregation, and help them regain normal functionfollowing stress [41]. In this study, HSP 21 levelsincreased in both SA-treated and control leaves during heat stress (Fig.7). Under ... in photosynthesis, but instead due to heat stress. SA did not alter Pnsignificantly in plantsmaintained at the normal grow th temperature, but itmitigated the decrease in Pn under heat stress...

Báo cáo khoa học: Salicylic acid and the hypersensitive response initiate distinct signal transduction pathways in tobacco that converge on the as-1-like element of the PR-1a promoter pot

... Tobaccotranscription factor TGA2.2 is the main component of as-1-binding factor ASF-1 and is involved in salicylic acid- and auxin-inducible expression of as-1-containing target promoters. J. Biol.Chem. ... coordinated induction of aheterogeneous group of proteins in the infected and uninfected leaves, commonly referred to as pathogenesis-related (PR) proteins.PR proteins were first described in ... beenshowntobindtothismotifin vitro [27–29], and aninducible in vivo footprint has revealed significant changes in DNA accessibility to the identified element upon SARinduction [26]. In addition,...

Báo cáo khoa học: Amino acid residues on the surface of soybean 4-kDa peptide involved in the interaction with its binding protein potx

... in maintaining the hairpin-b during interaction withsolvent.Interaction of insulin with the 43-kDa proteinSimilarly to the 4-kDa peptide, insulin is able to interactwith the 43-kDa protein ... Tager, H.S. (1987) Role of the COOH-terminal B-chain domain in insulin–receptor interactions:identiﬁcation of perturbations involving the insulin mainchain.J. Biol. Chem. 262, 12054–12058.20. ... the phenyl-alanine B25 side chain during insulin–receptor and insulin–insulininteractions. Biochemistry 30, 8222–8229.2592 K. Hanada et al. (Eur. J. Biochem. 270) Ó FEBS 2003 and the dissociation...

Báo cáo khoa học: Okadaic acid induces DNA fragmentation via caspase-3-dependent and caspase-3-independent pathways in Chinese hamster ovary (CHO)-K1 cells pot

... totalprotein. Lower panel: percentage of PS-exposing cells (annexinV+⁄ 7-AAD) and annexin V+⁄ 7-AAD+) was determined by doublestaining with annexin–phycoerythrin to detect PS-exposing cells, and ... cells and examined the effects of gene knockout and treatment with caspase-3 inhibitors. Okadaic acid (OA) is a potentinhibitor of the serine⁄ threonine protein phosphatases (PPs) PP1 and PP2A, ... knockout or inhibitionof caspase-3 activity is ineffective in preventingOA-induced DNA fragmentation in CHO-K1 cells,z-DEVD-fmk was successful at inhibiting OA-inducedDNA fragmentation in caspase-3)...

$Báo cáo khoa học: Protective effect of active oxygen scavengers on protein degradation and photochemical function in photosystem I submembrane fractions during light stress pdf$

Báo cáo khoa học: Protective effect of active oxygen scavengers on protein degradation and photochemical function in photosystem I submembrane fractions during light stress pdf

... proteins indicating mainly1O2was involved[28]. In spinach thylakoids, illumination at low lightintensity resulted in the degradation of PsaB geneproduct into two fragments of 51 and 45 ... better insight into the photo-inducedchanges in PSI. The submembrane particles used con-tain all the components of PSI, including the cyto-chrome b6 ⁄ f complex and plastocyanin [31,32]. In thepresent ... structural perturbationsinvolved altered pigment–protein and protein–proteininteraction, contributing to the observed decreasedefﬁciency of energy migration and capture, and leadingto Chl degradation.Experimental...

Báo cáo khoa học: The heterogeneity of mast cell tryptase from human lung and skin Differences in size, charge and substrate afﬁnity ppt

... hydrogenperoxide.Lectin binding studiesFollowing the standard blotting procedure, filters wereheated and blocked at 56 °C for 30 min in 100 mL TBScontaining 2% (w/v) BSA, then 0.2 mL Tween 20 wasadded and incubation ... purification and specificity of wheat germ agglutinin. Biochem. J. 131, 155–162.48. Gallagher, J.T., Harding, M. & Dale, R.E. (1983) Sialic acid as acell surface binding for wheat germ agglutinin. In ... for45 min at a concentration of 5 lgÆmL)1,andtheblotswashed and incubated with diaminobenzidine and hydrogenperoxide. A combination of the biotinylated lectins Sambu-cus nigra agglutinin (SNA;...

Báo cáo khoa học: "Effects of Electroacupuncture on Minimum Alveolar Concentration of Isoflurane and Cardiovascular System in Isoflurane Anesthetized Dogs" docx

... analysis and spirometry module (M-CaiOV, Datex-Ohmeda, Helsinki, Finland) of the anesthetic patient monitoringsystem (S-3, Datex-Ohmeda, Helsinki, Finland). MACdeterminations were made according ... experiments for recovery and anestheticclearance. MAC of isoflurane and cardiovascularparameters were determined after EA at nonacupoint and and at acupoints LI-4, SP-6, ST-36 and TH-8.Electroacupuncture ... systemIntroductionIsoflurane was developed in 1965 and approved in thelate 1970’s in many countries. It has been widely used in veterinary and human practice because of its chemicalstability and...

Báo cáo khoa học: " Multilobular tumour of the caudal cranium causing severe cerebral and cerebellar compression in a dog" doc

... frequently found in dogs, MTB has been reported in humans [5], cats [5], a horse and a ferret [3]. The clinical signs depend on the tumour location and aggressiveness, and may include neurological ... 6 cm in diameter concerning the right occipital, parietal and temporal bones and protruding from the right occipital bone (Figs. 1A and B). The mass severely compressed the cerebellum and the ... in the veterinary literature concerning MTB, due to its rarity, varying biological behaviour and varying levels of matrix production that may include osteoid tissue, bone or /and cartilage....

báo cáo khoa học: "Analysis of the expression pattern of the BCL11B gene and its relatives in patients with T-cell acute lymphoblastic leukemia" doc

... Guangdong Province, PR China.RNA extraction and cDNA synthesisRNA was extracted using the Trizol kit (Invitrogen,Carlsbad, CA, USA) and reverse transcribed into thefirst-strand cDNA using random ... 6FAM-TAMRA probe, and PCR buffer containing 4.5 mMMgCl2. After an initial denaturation at 95°C for 5 min,50 cycles consisting of 95°C for 15 s and 64°C for 1 minwere performed. Primers and probes ... wasperformed in a 25-μL total volume containing 1 μLofcDNA, 9 μL of 2.5× SYBR Green mix (Tiangen, Beijing,PR China), and 10 μmol/L primer pairs. The followingcycling conditions were used: initial...

báo cáo khoa học: "The use of some nanoemulsions based on aqueous propolis and lycopene extract in the skin’s protective mechanisms against UVA radiation" pps

... is standardized in polyphenol car-boxylic acids (caffeic acid) , responsible, among otheractive substances, for its healing and anti-inflammatoryaction upon tegument affected by dandr uff and ... incidence ofskin cancer has increased alarmingly. Exposure to UVirradiation has instantaneous effects (erythema and pig-mentation) and delayed effects (premature skin ageing and differe nt ... lycopene domain, using internal standardcalibration as calibration model. Lycopene area and standard area ratio were calculated for seven leve ls ofconcentration in the selected domain and used...

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