... plasmas and aborted fetuses of pigs.Materials and MethodsViruses and sample preparation The JEV isolate KV1899, Anyang 300 (attenuatedvaccine strain) and Nakayama strain were used as standardvirus ... byutilizing the 5-3 exonuclease activity of Thermus aquaticusDNA polymerase. Proc Natl Acad Sci USA 1991, 88, 7276-7280.10.Igarashi A, Tanaka M, Morita K, Takasu T, Ahmed A, Ahmed A, Akram ... generated by cleavage of the probe passed a fixed threshold above baseline. Standard deviation 10 abovebase line was used to determine the fixed threshold. For analysis of Ct values, less than 20 cycles...