báo cáo khoa học: " Transcriptomic analysis of pluripotent stem cells: insights into health and disease" potx

... 2011.doi:10.1186/gm284Cite this article as: Yeo J-C, Ng H-H: Transcriptomic analysis of pluripotent stem cells: insights into health and disease. Genome Medicine 2011, 3:68.Yeo and Ng Genome Medicine 2011, 3:68 http://genomemedicine.com/content/3/10/68Page ... pluripotent stem cells, pluripotency, regenerative medicine, therapy, transcriptional regulation, transcriptomics.© 2010 BioMed Central Ltd Transcriptomic analysis of pluripotent stem cells: insights ... self-renew, and to dierentiate into all cell types of the body. This unique capacity of ESCs and iPSCs to form all cell lineages is termed pluripotency. While ESCs and iPSCs are pluripotent and...

báo cáo khoa học: " Transcriptomic analysis of tomato carpel development reveals alterations in ethylene and gibberellin synthesis during pat3/pat4 parthenocarpic fruit set" pps

... a carpel into a fruit, and howthese processes can take place in absence of pollination. Transcriptomic analysis of tomato carpel development and fruit setTo identify the key steps and processes ... relevant and asses the impor-tance of GAs for parthenocarpic development and a newrole for ethylene in this process.Results Transcriptomic analysis of tomato carpel development and fruit ... auxins synthesis and the activation of fruit set, which innormal anthesis is in a state of temporary dormancy.Conclusion Transcriptomic analysis of tomato carpel development and fruit set provides...

báo cáo khoa học: "mRNA analysis of single living cells" docx

... contributionsTO conceived of the study and drafted the manuscript.HU carried out PCR and AFM. HK carried out AFM. AI par-ticipated in the design of the study and coordination. Allauthors read and approved ... 1: The detection of β-actin mRNA from single VNOf90 cells.The number of assays against single cellsThe number of β-actin positive casesThe number of cells The total number of β-actin positive ... sometimes fluctuate up and down. We expectthat these phenomena will aid in the understanding of theproperties of individual cells.MethodsPreparation of cellsRat fibroblast-like VNOf90 cells derived...

Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx

... acquired in H2O and D2O. Processing of the spectra was performed using the pro-gram TOPSPIN (Bruker). Analysis of the spectra, manualassignment of backbone and side-chain protons, and cross-peak ... pair of protons were converted into upper limits by cyana. Additionally, standard upper and lower limits for each of the two disulfide bonds (6–129, 57–108) were introduced during the rounds of ... structure of hirsutellin A – new insights into theactive site and interacting interfaces of ribotoxinsAldino Viegas1, Elias Herrero-Galań2, Mercedes Onãderra2, Anjos L. Macedo1 and Marta...

báo cáo khoa học: " Transcriptomic identification of candidate genes involved in sunflower responses to chilling and salt stresses based on cDNA microarray analysis" pps

... leaf, stem and flower at R1 and R4 developmental stage. A statistical analysis based on meancomparison by ANOVA and ordination by Principal Component Analysis allowed the detection of 80 candidate ... salinityProfile of gene expression by putative functional categoryFigure 5Profile of gene expression by putative functional category. The number and direction of transcriptional changes of the ... availability of Pi and phosphorylated intermediates in both the pathwayfor Suc synthesis and the Calvin cycle, and increasedcapacities of enzymes in both pathways [72]. However,one consequence of these...

Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

... Centre of Neuroscience, University of Insubria, Busto Arsizio, Italy2 Department of Biotechnology, University of Verona, Italy3 Department of Clinical and Biological Sciences, University of Insubria, ... Japan) and analyzed withimagemaster 2d platinum software, version 5.0 (GEHealthcare). Spots were detected automatically by the soft-ware and manually reﬁned; gels were then matched and theresulting ... manually excised and destained(1 · 10 min 50 lLofK3[Fe(CN)6]30mm and Na2S2O3100 mm;6· 10 min 100 lL of deionized water; 1 · 20 min100 lLofNH4HCO3200 mm;1· 20 min 100 lL of deion-ized...

Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf

... siteconcentration of wild-type BuChE was 0.16 ± 0.01 lm, and that of the D70G mutant was 0.196 ± 0.02 lm.Effects of ligands on steady-state kineticsThe effects of the ligands (L: tyramine, serotonin and ... ester).Results and DiscussionAction of reversible effectors on AAA activity of BuChEThe investigation of the effects of the ligands(tyramine, serotonin and benzalkonium) on the AAA and esterase ... the effects of thesecompounds on the AAA and esterase activities of human BuChE.The concentrations of tyramine and serotonin thatactivate or inhibit the AAA activity of BuChE (and also its...

Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... plastoquinone; PS, photosystem; QA and QB, primary and secondary quinone acceptors of photosystem II; t1 ⁄ 2 O–J, t1 ⁄ 2 J–I and t1 ⁄ 2 I–P, half-times of O–J, J–I and I–P phases, respectively.4770 ... thylakoidmembranes. Such analysis is needed for a a more thor-ough use of FI in the study of PSII electron transport and to obtain a more complete analysis of the O–J,J–I and I–P rises. This method ... superposition of the exponential rise to analyzequantitatively the contribution of each phase. Theamplitude and rate constant of each of the threephases can be calculated by deconvolution of thetraces...

Tài liệu Báo cáo khoa học: Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin pptx

... structure of MCL, and effect of porphyrin bindingCD spectra of MCL recorded in the far-UV region and near-UV region are given in Fig. 7A and 7B, respectively.Spectra obtained in the presence of a ... absorption bands.Fig. 2. (A) Absorption s pectra of CuTCPP in the absence and presence of different concentrations of MCL and (B) difference absorption spectraobtained by subtracting the spectrum of CuTCPP ... the presence of d ifferent c oncentrations of MCL. Tempera-ture ¼ 20 °C.Fig. 3. (A) Absorption spectra of CuTMPyP in the absence and pres-ence of different concentrations of MCL and (B) difference...

Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf

... ÔStains-AllÕ analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4.As the full-length Dp427 isoform of dystrophin does notenter the second dimension of conventional 2D gels, theexpression level of ... viscosity of t he extract as a result of D NA, 2 lLofDNase I (200 units) was added per 100 lL of buffer [30].Following ﬁltration through two layers of miracloth a nd theaddition of four volumes of ... alsequestrin ( CSQ), and calmodulin (CAM), respectively. The pH values of the ﬁ rst dimensiongel system and molecular mass standards (in kDa) of the seconddimension are indicated at the top and on the...

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