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báo cáo khoa học: " High-throughput sequencing identifies STAT3 as the DNA-associated factor for p53-NF-κB-complex-dependent gene expression in human heart failure" pps

Báo cáo khoa học: Fatty acid omega-oxidation as a rescue pathway for fatty acid oxidation disorders in humans pot

Báo cáo khoa học: Fatty acid omega-oxidation as a rescue pathway for fatty acid oxidation disorders in humans pot

... in amma-tion. The pro -in ammatory cytokines interleukin-1b,interleukin-6 and tumor necrosis factor- a are able toinduce CYP4Fs, whereas the anti -in ammatory cyto-kine interleukin-10 suppresses CYP4F expression ... known as peroxisome prolifer-ator responsive elements in the promoter regions oftarget genes, thereby inducing gene expression of the target gene. Most of these target genes are involved in lipid ... saturatedlong-chain FAs. These include very long-chain acyl-CoA dehydrogenase, medium-chain acyl-CoA dehy-drogenase and short-chain acyl-CoA dehydrogenase. The same is true for the third step in mitochondrialfatty...
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Báo cáo khoa học: Effects of juvenile hormone on 20-hydroxyecdysone-inducible EcR, HR3, E75 gene expression in imaginal wing cells of Plodia interpunctella lepidoptera ppt

Báo cáo khoa học: Effects of juvenile hormone on 20-hydroxyecdysone-inducible EcR, HR3, E75 gene expression in imaginal wing cells of Plodia interpunctella lepidoptera ppt

... aregivenontheleftandtheaminoacidnumberson the r ight. Letters in the r ight margin des-ignate d omains. The DNA binding domain(C region) is underlined and the ligand bindingdomain (E region) is u nderlined with dashes. The helix–turn–zipper ... cells for the expression of PIEcR-B1 and PHR3.Therefore,inthepresence of JH II, the increased accumulation of PH R3mRNA could inhibit the second increase in PIEcR-B1mRNA and block the 20E-induced ... th e initial 20E-induced increase in PIEcR-B1 mRNA whereas it p revented the second increase.This result was agreement with that obtained on EcRhomologous gene in the M. se xta epidermis. In...
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Báo cáo khoa học:

Báo cáo khoa học: "Upper abdominal body shape is the risk factor for postoperative pancreatic fistula after splenectomy for advanced gastric cancer: A retrospective study" ppsx

... POPF in this study. In contrast, we found that the shape of the upper abdomen significantly correlated withPOPF. One of the reasons for the inconsistent resultsmight be the difference in the ... takingparticular care to avoid injuring the pancreas. The splenicartery is divided at the end of the pancreatic parenchyma. The splenic vein is ligated and resected at the same level as the ... incidenceof intraabdominal complication such as anastomoticleakage or POPF. In case of having intraabdominal infec-tious complication, we changed drains under radio-graphic examination and lavaged the...
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báo cáo khoa học:

báo cáo khoa học: " Deep sequencing identifies novel and conserved microRNAs in peanuts (Arachis hypogaea L.)" pps

... miRNA genes,located mainly in the intergenic genomic region, byRNA polymerase II [10-12]. There are also a small num-ber of miRNA genes located inside the protein codinggenes. For these miRNAs, ... length for a majority of the small RNAs. Combining the deep sequencing andbioinformatics, we discovered 14 novel miRNA families as well as 75 conserved miRNAs in peanuts. All 14 novelpeanut miRNAs ... and development. In co ntrast, miRNAs nega-tively control gene expression by targeting protein cod-ing gene mRNAs for cleavage o r repressing proteintranslation [2,3].miRNAs are first transcribed...
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Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt

Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt

... conservedfunctional modules: the DNA-binding domain (DBD)at the amino-terminus and the oligomerization domain in the central region of the protein [1,4]. The HSFDBD belongs to the ‘winged’ helix-turn-helix ... Unlike other winged helix-turn-helix proteins, the wing of the HSF does not appear to contact the DNA. The wing of the Saccharomyces HSF is necessary for efficient binding of a single trimer to the ... DBD–DBD interaction is important in the HSF–HSF and HSF–HSE interactions. In HSF DBD–HSE co-crystals, the protein–protein interface consistsof the helix 2 amino-terminus, the turn and the wing[15]....
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Báo cáo khoa học: Semi-nested PCR analysis of unknown tags on serial analysis of gene expression potx

Báo cáo khoa học: Semi-nested PCR analysis of unknown tags on serial analysis of gene expression potx

... of genes predicted by Zhao based on the spermatozoa SAGE tags [12], whereas no. D was notmatched to the gene (Hs. 436980). The reason for thiswas further investigated, and it was found that the no. ... PCR using only a single primer (the tag-specific primer no. 10). Sequencing could onlydetermine the single primer-prone product. The sequencing results (Table 1) were analyzed using the blast program ... pro-cess, these nucleotides position the primer at the startof the poly(A)+tail, thereby eliminating the 3¢-heter-ogeneity inherent in conventional oligo(dT) priming[14]. As the PrimeScript...
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Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt

Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt

... °CÆmin)1. The melting point (Tm) was obtaineddirectly from the DSC curve. The enthalpy change (DHcal)was calculated by the integration of the curve covering area(Tmwas taken as the ... exists in these mutants (Fig. 1).Thermal analysis of protein unfoldingThermal denaturation determined by DSC was used tomeasure the stability of the mutant proteins. Based on the measurements ... protein unfold-ing for wild-type SNase and 20 glycine mutants suggestthat the negatively charged aspartic acid and glutamicacid residues in the protein play unequal roles in main-taining the...
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Báo cáo khoa học:

Báo cáo khoa học: "Using Similarity Scoring To Improve the Bilingual Dictionary for Word Alignment" doc

... example, if the maximumnumber is 2, then a word can align to 0, 1, or 2 words in the parallel sentence. In other settings, we en-forced a minimum score in the bilingual dictionary for a link to ... words. The similarity score of and is then computed using the following formula:This similarity scoring provides the basis for ournewly built dictionary. The algorithm proceeds as follows: For ... 50performanceminscore’Precision3-Dict0.02-Cog7.3’’Precision3-Dict0.02-Baseline’’Recall3-Dict0.02-Cog7.3’’Recall3-Dict0.02-Baseline’’F-measure3-Dict0.02-Cog7.3’’F-measure3-Dict0.02-Baseline’Figure 9: Performance of dictionaries Dict0.02 for up to three links per wordProceedings of International Workshop on Informa-tion...
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Báo cáo khoa học: Directed evolution of formate dehydrogenase from Candida boidinii for improved stability during entrapment in polyacrylamide ppt

Báo cáo khoa học: Directed evolution of formate dehydrogenase from Candida boidinii for improved stability during entrapment in polyacrylamide ppt

... °C for 30 min. The reactionwas started by injecting 100 lmol of formate into the vialand stopped after 60 min by injecting 1 mL of HCl(1 molÆL)1). After another 30 min of incubation, a gas ... mmolÆL)1NAD+ in 0.1 molÆL)1potassium phos-phate buffer (pH 7.5). The reaction was started by addingFDH and was monitored over 2 min. From the increase in extinction, activity was calculated using the ... second generation increasedwith increasing concentration of PAA. These findingswere in very good accordance with the behaviour of the variants under screening conditions. Of course,based on the...
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Báo cáo khoa học: Ets-1/ Elk-1 is a critical mediator of dipeptidyl-peptidase III transcription in human glioblastoma cells pdf

Báo cáo khoa học: Ets-1/ Elk-1 is a critical mediator of dipeptidyl-peptidase III transcription in human glioblastoma cells pdf

... immunoprecipitation assays. Mutationsof these binding sites abolished not only binding of the Ets protein, butalso the intrinsic promoter activity. Increased DNA-binding activity ofEts-1 ⁄ Elk-1 by v-Ha-ras ... in the 5¢ flanking regionof the human DPP-III gene in different cell lines. The PCR-amplified5¢ flanking region of human DPP-III gene (1038 bp) was clonedupstream of the luciferase reporter gene ... regulation of the gene. In thisregard, we identified the region that plays an impor-tant role in determining the basal promoter activity of the gene. Furthermore, with the help of binding assaysand...
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