báo cáo khoa học: "The ACR11 encodes a novel type of chloroplastic ACT domain repeat protein that is coordinately expressed with GLN2 in Arabidopsis" pptx

... the ACT domains of the ACR11 andACR12 proteins may serve as amino acid bindingdomains. Upon binding to specific amino acids, the ACR11 and ACR12 proteins may regulate the activities of amino acid ... of four novel ACT domain- containing proteins, namely ACR9 to ACR12, in Arabidopsis. The ACR9 and ACR10 proteinscontain three copies of the ACT domain, whereas the ACR11 and ACR12 proteins have ... glutamine [21].The ACT domain, named after bacterial aspartatekinase, chorismate mutase and TyrA (prephenate dehy-drogenase), is a regulatory domain that serves as anamino acid-binding site in...

Tài liệu Báo cáo khoa học: ˚ The 1.8 A crystal structure of a proteinase K-like enzyme from a psychrotroph Serratia species docx

... domain and two similar C-ter-minal domains with unknown structure and function.The N-terminal pro domain is autocatalytically cleavedoff when the enzyme has obtained its active conforma-tion, ... and the two C-terminal domains are cleaved offby heat treatment at 50 °C. An Ala-Pro-Thr sequence is located in the ﬁrst C-terminal domain. The tripeptide is not located in the primary bindingsite ... peptidases [1]. Neutral pep-tidases are active within a relatively narrow range(pH 5–8) and are often used in the food industry.Alkaline peptidases are generally highly active aroundpH 10, have...

Tài liệu Báo cáo khóa học: The PAS fold A redeﬁnition of the PAS domain based upon structural prediction ppt

... thaliana proteins alsocontain a PAC motif, and conversely that all PAC-annotated A. thaliana proteins contain a PAS domain. Therefore, in the case of A. thaliana,thePASandPACmotifs are inseparable, ... future.3Arabidopsis,Escherichia,CaenorhabditisandAzotobacter– a case studySome of the PAS domains have been analysed in detail.We chose four representative organisms from the animal,bacterial and plant kingdoms, A. thaliana, ... contain a PAS domain based upon amino acid sequence alignments. One of the problems with the current annotation of the PAS domain is that this domain exhibits limited similarity at the aminoacid...

Báo cáo khoa học: The calpain 1–a-actinin interaction Resting complex between the calcium-dependant protease and its target in cytoskeleton doc

... Location of the bindingstructures shows that the C-terminal domain of a- actininand each calpain subunit, 28 and 80 kDa, participates in the interaction. In particular, the autolysed calpain form(76/18) ... the cleavage of asm by calpain 1 generates a fragment of 95 kDa which is unable to interact with Calp1.This indicates the presence of a calpain binding site in theC-terminal domain of smooth ... muscle a- actinin;Calp1,calpain1(l-calpain); Calp2, calpain 2 (m-calpain);CaM, calmodulin; ELISA, enzyme-linked immunosorbant assay;FITC, ﬂuorescein 5-isothiocyanate; Seph-ask, Sepharose-insolubilizedskeletal...

Báo cáo khoa học: Oxidative stress induces a reversible ﬂux of cysteine from tissues to blood in vivo in the rat pptx

... Indeed, the factors that main-tain the redox homeostasis of plasma thiols as well asthe mechanisms that regulate the exchange of LMM-SH among cells need to be clariﬁed.Here, we have analyzed the ... levels increased in plasma, indicating that Cys wasreleased from an extra-hematic compartment.As in vivo studies based on treatments with diamideare essentially limited by its reaction with ... and blood withdrawal. Blood was withdrawn 15 minafter the end of diamide infusion and maintained at 37 °C. Timezero indicates the time of withdrawal. Data are the means ± SD of three experiments....

Báo cáo khoa học: The molecular chaperone a-crystallin incorporated into red cell ghosts protects membrane Na/K-ATPase against glycation and oxidative stress ppt

... show that the protection that a- crystallin providedagainst Na/K-ATPase inactivation was not due to theremoval of fructose by binding to a- crystallin, BSA wasresealed (in the same manner and ... test inactivation of the Na/K pump. Intracellular a- crystallin protected against the decrease in ouabain sensi-tive86Rb uptake, and therefore against inactivation inducedby all external modiﬁers, ... of a range of enzymes in vitro [7–9], andthe inactivation was prevented by a- crystallin. a- Crystallin, a lens structural protein, comprising of aAandaB subunits is a ubiquitous molecular chaperone,...

Báo cáo khoa học: The consensus motif for N-myristoylation of plant proteins in a wheat germ cell-free translation system ppt

... fused with a DNA fragment encoding themyristoylation motif Met-Gly-Ala-Ala-Ala-Ala-Ala-Ala-Ala-Ala or Met-Gly-Ala-Ala-Ala-Ser-Ala-Ala-Ala-Ala wasampliﬁed by PCR with the primers AGG1 RV and 3A6 (A ... N-terminus to the myristoylationmotifs Met-Gly-Xaa-Ala-Ala-Ala-Ala-Ala-Ala-Ala(Myr–AGG1-3X 6A) or Met-Gly-Xaa-Ala-Ala-Ser-Ala-Ala-Ala-Ala (Myr–AGG1-3X6S), with position 3 of each motif separately ... Structure of mature AGG1 fused at its N-terminus to themyristoylation motifs Met-Gly-Xaa-Ala-Ala-Ala-Ala-Ala-Ala-Ala (Myr–AGG1-3X 6A) orMet-Gly-Xaa-Ala-Ala-Ser-Ala-Ala-Ala-Ala(Myr–AGG1-3X6S)....

Báo cáo khoa hoc:" The chicken as a model to study microchromosomes in birds: a review" potx

... is essential to allow a precise localization of genes and markers,leading to completed genetic maps. For this purpose, a collection of large insertBAC (bacterial artificial ... authors, the actual standard karyotype description by GTG- andRBG-banding for the chicken, established by the International Committeefor the Standardization of the Avian ... R., Billault A. , Severac V., Fillon V., Vignal A. , Auffray C., Twochicken genomic libraries in the PAC and BAC cloning systems: organization andcharacterization, Anim. Genet....

báo cáo khoa học:" The ICF as a common language for rehabilitation goal-setting: comparing client and professional priorities" ppt

... clientsregarding the important domains for inclusion indicate that this is a reliable method f or assisting individuals with an acquired communication disability to participate in the identification of ... ajority of the domains which are viewed as important for inclu-sion at this stage of the rehabilitation process can beclassified as activity domains (learning and thinking,Harty et al. Health ... and participation domains.Certain authors state that a lack of conceptual clarityexists between the concept of activity and the concept of participation as operationalized within the ICF, andargue...

Báo cáo khoa học: The product chain length determination mechanism of type II geranylgeranyl diphosphate synthase requires subunit interaction pptx

... geneencoding GGPS was ampliﬁed using PCR with KOD DNApolymerase (Toyobo, Osaka, Japan) and the primers: PaGGPS-Fw, 5¢-AAGAAACATATGACGGTCTGCGCAAAAAAACACG-3¢, and PaGGPS-Rv, 5¢-TGCAGAGGATCCTTAACTGACGGCAGCGAGTTTTTTG-3¢. ... interface. It is conceivable that a similarscenario also occurred in the I12 1A and V12 5A mutants of P. ananatis GGPS.The role of a- helix E in the mechanism of chain lengthdetermination also has ... deter-mine whether this mechanism of chain length deter-mination also operates in type II GGPS fromP. ananatis, alanine 89 at the ﬁfth position upstreamfrom FARM was replaced with bulky amino acids(Fig....

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