Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx

... selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase Wayne M. Patrick1,2 and Jonathan M. Blackburn1,3,41 Department of Biochemistry, University of Cambridge, ... of trPRAI-HisThe far-UV and near-UV CD spectra of trPRAI-Hiswere recorded and analyzed in a manner identical withthose for PRAI and FLAG-PRAI (vide supra). In addi-tion, the thermal melting ... thermostable and monomeric, implying that it repre-sents a genuine subdomain of PRAI and providing further evidence thatsuch subdomains have played an important role in the evolution of the(ba)8-barrel...

Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf

... stimulation effect of DDI- 1A containing a right-handed aglycon helix wasgreater than that of DDI-1B; (e) the enhancementeffects of DDI- 1A and DDI-1B on AAT, CAG and CA repeats are stronger than ... polynucleotide kinase.DNA polymerase assays A standard reaction (15 lL) contained 4 lm each of theprimer and template and 1 mm each of deoxynucleoside tri-phosphate, DNA polymerase and the corresponding ... not affect DNA slippage (data notshown). Interestingly, there was a similar hierarchy of intensities for the three bands in the (AAT)3⁄ (ATT)5 and (ATT)3⁄ (AAT)5systems (Fig. 1A, B), each...

Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf

... Calcaterra NB, Palatnik JF, Bustos DM, Arranz SE &Cabada MO (1999) Identiﬁcation of mRNA-bindingproteins during development: characterization of Bufoarenarum cellular nucleic acid binding ... wereadded to a ﬁnal concentration of  2nm, and accuratemolar amounts of fusion proteins were added as indicated.Final reaction volume was 20 lL. Binding reactions wereincubated for 30 min at ... protein. DevGrowth Differ 41, 183–191.12 Yasuda J, Mashiyama S, Makino R, Ohyama S, SekiyaT & Hayashi K (1995) Cloning and characterization of rat cellular nucleic acid binding protein (CNBP)...

Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx

... cleavages by beta-site APP cleaving enzyme and c-secretase to form amyloid-b (Ab). The beta-siteAPP cleaving enzyme cleavage releases an APP ecto-domain leaving a 99-amino acid membrane spanningC-terminal ... 5¢-GGGGGGCCATGGCGACAGTGATCGTC-3¢; reverse, 3FLAG HindIIIcreating the plasmid c-3FLAG standard. Finally, the pri-mer pairs forward, 5¢-GGGGGGCCATGGTGATGCTGAAGAAGAACAG-3¢ and reverse 3FLAG HindIII ... [10,11], and haveaided in the ﬁnding of activity-modulating factors[12,13]. These assays have shown that peripheral mem-brane proteins are not necessary for activity as car-bonate washing retains...

Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc

... AnI19R5¢-AATTCATGAGGAGGTTTCTCTGTAACA-3¢;AnI19H5¢-AGCTTGTTACAG-AGAAACCTCCTCATG-3¢; AnI17R5¢-AATTCACGAGGAGGTTTCTCTGTACTA-3¢;AnI17H 5¢-AGCTTAGTACAGAGAAACCTCCTCGTG-3¢;AnI15R5¢-AATTCACAGGAGGTTTCTCT-GTCTA-3¢;AnI15H5¢-AGCTTAGACAGAGAAACCTCCTGTG-3¢. Each annealed ... Coralville, IA, USA)were annealed together to make recognition site variantscontaining 5¢-EcoRI and 3¢-HindIII sticky ends: AnI19R5¢-AATTCATGAGGAGGTTTCTCTGTAACA-3¢;AnI19H5¢-AGCTTGTTACAG-AGAAACCTCCTCATG-3¢; ... thecomplete inability of a saturating amount of DNA substrateto inhibit RNA splicing (Fig. 6B) and therefore it seemsreasonable to make the working hypothesis that the RNA and DNA binding sites are...

Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot

... maintain the active SPase I at the highest level in the exponential growth phase.MATERIALS AND METHODSMaterials and bacterial strainsRestriction enzymes, T4 DNA ligase and Taq DNApolymerase ... template and two oligonucleo-tides as primers (5¢-GCAATGTTCGCGTACATATGCATTCCATGGATCCGACC-3¢ and 5¢-TGGTGGTGCTCGAGAAATGTTCCGATACGGGTGATTGGCCAGAAGCG-3¢), which were designed to contain NdeIandXhoIrestriction ... the membranewere excised and the N-terminal amino acid sequence of each protein was determined by automated Edmandegradation.Cleavage of prestreptokinase byS. pneumoniaeSPase I and SPase37–204We...

Báo cáo khoa học: "Investigating Cue Selection and Placement in Tutorial Discourse" ppt

... this analysis into a database. The technique of representing an analysis in a database and then using database queries to test hypotheses is similar to work using RST analyses to investigate ... have applied RDA to our corpus of tutorial explanations, producing an exhaustive analysis of each explanation. By doing such an extensive analysis and representing the results in a database, ... appears as the mother of all the relations it participates in. Each relation is labeled with both its intentional and informational relation, with the order of relata in the label indicating the...

Báo cáo khoa học: Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis A key factor participating in natural rubber biosynthesis pdf

... (5¢-GCAAATGCAACTGGAAGCGG-3¢)andA1(5¢-ACAGCCTGCTAGCAAAGAGG-3¢) for amplification of HRT1, and primers S2(5¢-GAAGAATCCTCTAAGGATAA-3¢)andA2(5¢-TACAAGGATTAATCCCTTGC-3¢) for amplification of HRT2. ... Wititsuwannakul4,Seiji Takahashi1, Atiya Rattanapittayaporn4 and Tanetoshi Koyama11Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Japan;2Department ... containing 50 pmol of amplification primer pair for 45 cycles of 30 s at 95 °C, 30 sat 45 °C and 1 min at 72 °C with a 5 min preheat and a 10 min final extension at 72 °C. The resulting band of...

Báo cáo khoa học: "Test Collection Selection and Gold Standard Generation for a Multiply-Annotated Opinion Corpus" potx

... High agreement To see how the generated gold standards agree with the annotations of all annotators, we analyze the kappa value from the agreements of each anno-tator and the gold standard ... N3.3 Kappa value for agreement We further assess the usability of the annotated corpus by Kappa values. Kappa value gives a quantitative measure of the magnitude of inter-annotator agreement. ... are those sentences which are annotated as opinionated by all three annotators, while cases D, E are those sentences which are annotated as opinionated only by two annotators. In case A and...

Báo cáo khoa học: "SVM Model Tampering and Anchored Learning: A Case Study in Hebrew NP Chunking" pdf

... Anchored Learning is toadd a unique feature a i(an anchor) to each trainingsample (we add as many new features to the modelas there are training samples). These new featuresmake our data ... SVMchunker on anchored data (as the anchored data isguaranteed to be linearly separable, we can set averyhigh value to the C parameter, preventing any mis-classiﬁcation), and then investigating either ... adapting results established in English toanother language: (1) acquiring high quality anno-tated data; (2) adapting the English task deﬁnitionto the nature of a different language, and (3) adapt-ing...

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