Báo cáo khóa học: High level cell-free expression and specific labeling of integral membrane proteins doc
Báo cáo khóa học: High level cell-free expression and specific labeling of integral membrane proteins doc
... advan-tages of the cell-free expression of IMPs were the high level production of insoluble protein and the efficient selective labeling. This is the first report of the solubilization of SugE,YfiK and ... folded conformation of the proteins. Keywords: amino acid specific labeling; cell-free expression; integral membrane proteins; multidrug transporter; solutionNMR. Integral membrane proteins (IMPs) ... 73–80.580 C. Klammt et al. (Eur. J. Biochem. 271) Ó FEBS 2004 High level cell-free expression and specific labeling of integral membrane proteins Christian Klammt1, Frank Lo¨hr1, Birgit Scha¨...
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Tài liệu Báo cáo khoa học: Molecular cloning, recombinant expression and IgE-binding epitope of x-5 gliadin, a major allergen in wheat-dependent exercise-induced anaphylaxis ppt
... QQFPQQQ,QQSPEQQ, QQSPQQQ, QQYPQQQ and QQPPQQ.The serum of patient two had specific IgE antibodiesto QQIPQQQ, QQFPQQQ, QQSPEQQ, QQSPQQQ and YQQYPQQ. The serum of patient three had specific IgE antibodies ... shown inFig. 3 lane 2 a high level of expression of recombinantprotein, designated rO5GC, was obtained. The x-5gliadin purified from wheat flour is slightly soluble inwater and soluble in 70% ethanol, ... place. Therefore identification and charac-terization of major allergens for each clinical mani-festation is important and the use of standardizedrecombinant proteins might reduce inaccurate...
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Báo cáo khoa học: Voltage-gated sodium channel isoform-specific effects of pompilidotoxins doc
... tested VGSC isoforms.ResultsScreening of peptides for robustness of theslowing of the inactivation process both inmammalian isoforms and in one insect isoformAs only a limited amount of each compound ... analogs of PMTX and the eight VGSC isoforms. A maximalconcentration of 46 lM was used (n = 4 for each data point). See[17] and Experimental procedures for details of the sequences of the other ... potential of )110 mV in controls and duringperfusion of b-PMTX at 46 and 140 lM. (B) Fractional change of thefast and steady-state components versus [b-PMTX] (n = 4). (C)Dose–response curve of the...
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Báo cáo khoa học: The Janus-faced atracotoxins are specific blockers of invertebrate KCa channels ppt
... M. Nicholson and G. F. King, and DP0774245to G. F. King) and Australian Postgraduate Awardsto S. J. Gunning and M. J. Windley. Requests forsamples of J-ACTX-Hv1c and ⁄ or the expression system ... bonds and b-strands are shown in red and cyan, respec-tively. (D, E) Surface representation of J-ACTX-Hv1c (D) and ChTx (E), highlighting the primary pharmacophore residues. In the case of ChTx(a-KTx ... fetal bovine serum and l-glutamine (1 mm). Expression of pSlo and mSlo was performed by transfection of HEK293 cells with a construct containing the codingregion cloned into the expression vector...
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Báo cáo khoa học: Mutant recombinant serpins as highly specific inhibitors of human kallikrein 14 ppt
... & Dia-mandis EP (2003) Human kallikrein 13: production and purification of recombinant protein and monoclonal and polyclonal antibodies, and development of a sensi-tive and specific immunofluorometric ... development of highly specific inhibitors for hK14. Using two differentbackbones, we developed four recombinant inhibitors,two of which demonstrated high specificity. Prelimin-ary studies on hK14 expression ... afinal concentration of 0.5 mm and 0.1 mm for production of rACT proteins and rAAT proteins, respectively. Therecombinant serpins were expressed for an induction per-iod of 16 h at 18 ° C. Cells...
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Báo cáo khoa học: Bovine tryptases cDNA cloning, tissue specific expression and characterization of the lung isoform ppt
... Hellman, L. (1993) Expression of amast cell tryptase in the human monocytic cell lines U-937 and Mono Mac 6. Scand. J. Immunol. 38, 359–367.Ó FEBS 2003 Tissue -specific expression of bovine tryptases ... cloning, tissue specific expression and characterization of the lung isoformAlessandra Gambacurta1*, Laura Fiorucci1*, Paolo Basili1, Fulvio Erba1, Angela Amoresano2 and Franca Ascoli11Department ... model substrates are 10- to 60-fold higher in the case of BLT. The tissue -specific expression of the two tryptaseswas evaluated at the RNA level by analysis of their dif-ferent restriction patterns....
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Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc
... relative expression level of the GFP-tagged proteins is indicative of that of the equivalent untagged proteins. Lipid anchoring of C-Vrp1p bypasses therequirement for the LBD for endocytosis and growth ... but we expect that the relative expression level of the tagged proteins is indicative of that of the equival-ent untagged proteins. To assess the function of these proteins in endo-cytosis we ... a direct effect of the loss of residues465–492 rather than an indirect consequence of reduced C-Vrp1p493)817 expression levels. We were notable to assay the expression level of the untagged...
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Tài liệu Báo cáo khoa học: "Exploiting Readymades in Linguistic Creativity: A System Demonstration of the Jigsaw Bard" docx
... data-base of Web ngrams – snippets of Web text (of oneto five words) that occur on the web with a fre-quency of 40 or higher (Brants and Franz, 2006).Each is likely a literal description of a real ... comple-ment of adjectival properties used by Veale and Hao (2007), we harvest all instances of the patterns“as ADJ and * as” and “as * and ADJ as” fromGoogle, noting the combinations that are found and their ... form of creativity that is poorly served by exploratorymodels of creativity, such as that of Boden (1994), and better served by the investment models such asthe buy-low-sell -high theory of Sternberg...
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Báo cáo khoa học: The cytochrome P450scc system opens an alternate pathway of vitamin D3 metabolism docx
... Department of Biochemistry and Molecular Biology, School of Biomedical and Chemical Science, The University of Western Australia,Crawley, AustraliaThe predominant source of the main form of vitamin ... alternatepathway of vitamin D3 metabolismAndrzej Slominski1, Igor Semak2, Jordan Zjawiony3, Jacobo Wortsman4, Wei Li5,Andre Szczesniewski6 and Robert C. Tuckey71 Department of Pathology and ... furthermetabolism of dihydroxycholecalciferol. Based on NMR analysis and known properties of P450scc we propose that hydroxylation of vitamin D3by P450scc occurs sequentially and stereospecifically...
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Báo cáo khoa học: Dehydroepiandrosterone inhibits the proliferation and induces the death of HPV-positive and HPV-negative cervical cancer cells through an androgen- and estrogen-receptor independent mechanism pptx
... Montan˜oLF&Lo´pez-Marure R(2005) Dehydroepiandrosterone inhibits the prolifera-tion of HUVEC by enhancing the expression of p53 and p21, restricting the phosphorylation of RB, and isandrogen- and estrogen-receptor ... estrogen and androgen,because use of antagonists to androgen and estrogenreceptors (flutamide and ICI 182,780, respectively), and letrozol, an inhibitor of the aromatase responsiblefor converting androgen ... humans of both sexes, as the incidence of most cancers rises. Low levels of these adrenalsteroids have been associated with the presence and risk of development of cancer. Oral administration of DHEA...
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