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Báo cáo khoa học: Benzo[a]pyrene impairs b-adrenergic stimulation of adipose tissue lipolysis and causes weight gain in mice A novel molecular mechanism of toxicity for a common food pollutant doc

Báo cáo khoa học: Benzo[a]pyrene impairs b-adrenergic stimulation of adipose tissue lipolysis and causes weight gain in mice A novel molecular mechanism of toxicity for a common food pollutant doc

Báo cáo khoa học: Benzo[a]pyrene impairs b-adrenergic stimulation of adipose tissue lipolysis and causes weight gain in mice A novel molecular mechanism of toxicity for a common food pollutant doc

... The Authors Journal compilation ª 2006 FEBS Benzo [a] pyrene impairs b-adrenergic stimulation of adipose tissue lipolysis and causes weight gain in mice A novel molecular mechanism of toxicity for ... significant weight gain and increasedfat mass without detectable changes in food intake.Upon withdrawal of this PAH, the excess weight gain was not corrected.Our data indicate that inhibition of lipolysis ... that micromolar concentrations of a common food pollutant, B [a] P, caused a rapid,direct and profound inhibition of adipose tissue lipo-lysis stimulated by epinephrine, dobutamine, salbuta-mol,...
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Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt

Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt

... mitochond-rial b-ketoacyl-ACP synthase, bacterial plus plastid b-ketoacyl-ACPsynthases I and II, and a domain of human fatty acid synthase, have a Cys-His-His triad and also a completely conserved Lys in ... extracts of this strain are unableto extend radiolabeled acetate (lane 5) in 30 min assaysat 42 °C. Addition of wild-type KAS I resulted in syn-thesis of long-chain saturated and unsaturated ... vicinity of the active site (Fig. 2). Three of the six cation lig-ands are main-chain oxygen atoms, and three are theside-chain oxygen atoms of N296, E342 and S387.The glutamic acid and asparagine...
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Tài liệu Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products pptx

Tài liệu Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products pptx

... Watanabe,T.,Kobori,K.,Kiyotaka,Miyashita,Fujii,T.,Sakai,H. & Makot, Uchida and Tanaka, H. (1993) Identification of glutamic acid 204 and aspartic acid 200 in chitinase A1 of Bacilluscirculans WL-12 as essential residues for chitinase activity. ... which increases in molecular mass and DA resulted in efficient loading and minimization of therelease of entrapped lipase [30]. The observed increase in specific activity with increasing molecular ... precipitate (LMWC) obtainedafter centrifugation at 1000 g, was dialyzed against waterusing a membrane with a molecular mass cut-off of 2 kDa(Sigma Chemicals Co.) and freeze-dried.The supernatant...
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Báo cáo khoa học: Tumor suppressor p16INK4a ) modulator of glycomic profile and galectin-1 expression to increase susceptibility to carbohydrate-dependent induction of anoikis in pancreatic carcinoma cells ppt

Báo cáo khoa học: Tumor suppressor p16INK4a ) modulator of glycomic profile and galectin-1 expression to increase susceptibility to carbohydrate-dependent induction of anoikis in pancreatic carcinoma cells ppt

... (snowdrop) GNA Man Mana6(Mana3)ManaRGlycine max (soybean) SBA GalNAc GalNAca3Galb6GlcGriffonia simplicifolia I GSA I GalNAc GalNAca3Gal, GalNAca3GalNAcb3Gala4Galb4GlcGriffonia simplicifolia II GSA II ... Gal Galb3GalNAcaCanavalia ensiformis (jack bean) Con A Man ⁄ Glc GlcNAcb2Mana6(GlcNAcb2Mana3)Manb4GlcNAcDatura stramonium (thorn apple) DSA GlcNAc (GlcNAc)n, Galb4GlcNAcb6(Galb4GlcNAcb2)Man ... order.Latin name (common name) AcronymMonosaccharidespecificity Potent oligosaccharide a Artocarpus integrifolia (jack fruit) Jacalin (JAC) Gal ⁄ GalNAc Galb3GalNAcaArachis hypogaea (peanut) PNA...
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Báo cáo khóa học: Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line docx

Báo cáo khóa học: Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line docx

... it appears that at least three oligomannosidestructures may be involved as degradation signal for ERAD(Man8GlcNAc2 and Man6GlcNAc2 for glycoproteinsbearing Man9 species and Man4GlcNAc2 for ... Man9 glycans and glycoproteinsbearing Man5 glycans can be efficiently degraded. A widevariety of variables can lead to defective synthesis of lipid-linked oligosaccharides and, therefore, in ... Endoplasmic reticulum-associated degradation of glycoproteinsbearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5CHO cell lineFranc¸ois Foulquier, Sandrine Duvet, Andre´Klein, Anne-Marie...
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Báo cáo khoa học: Purple membrane lipid control of bacteriorhodopsin conformational flexibility and photocycle activity An infrared spectroscopic study docx

Báo cáo khoa học: Purple membrane lipid control of bacteriorhodopsin conformational flexibility and photocycle activity An infrared spectroscopic study docx

... Bose1 and Ira W. Levin21Laboratory of Cell Biology, National Heart, Lung, and Blood Institute and 2Laboratory of Chemical Physics,National Institute of Diabetes and Digestive and Kidney Diseases, ... dynamic parameters derived from intermolecular and intramolecular motions. Band shapes are often analyzed in the context of statistical mechanical theories of irreversibleprocesses. Interactive ... bacteriorhodopsinphotocycle data, after an actinic light flash, were obtained and analyzed as previously described [8,9].Infrared spectroscopic measurements were obtainedthroughfilmscastat35°ContoaBaF2window from75...
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Báo cáo khoa học: Discriminating between the activities of human cathepsin G and chymase using fluorogenic substrates potx

Báo cáo khoa học: Discriminating between the activities of human cathepsin G and chymase using fluorogenic substrates potx

... lineextract and in sputumMast cells contain substantial amounts of a variety of proteases, including chymase, tryptase, carboxypepti-dase A3 and dipeptidyl peptidase I (cathepsin C), thatparticipate ... does not cleave P1-Lys containing substrates [24] and, more recently, as a result of a phylogenetic analysis of mammalianCGs [37]. Human chymase also has an Ala residueat position 226 and this ... residue, although via a different mechanism thatinvolves Arg41 and Lys192 in CG and Arg143 and Lys192 in chymase. We have tested the in uence of negative and positive residues at P2¢ in the serpinB1-derived...
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Báo cáo khoa học: DYRK1A phosphorylates caspase 9 at an inhibitory site and is potently inhibited in human cells by harmine pptx

Báo cáo khoa học: DYRK1A phosphorylates caspase 9 at an inhibitory site and is potently inhibited in human cells by harmine pptx

... (Caspase 9) and DYRK1BSmartPool (Dharmacon) comprising four different duplexes:5¢-GAAAUUGACUCGCUCAUUGrUrU-3¢,5¢-ACACGGAGAUGAAGUACUArUrU-3¢,5¢-GCCAGAGGAUCUACCAGUArUrU-3¢,5¢-GCACAUCAAUGAGGUAUACrUrU-3¢. ... DYRK 1A (sense strands): 5¢-UAAGGAUGCUUGAUUAUGAdTdT-3¢(DYRK 1A# 1), 5¢-AAACUCGAAUUCAACCUUAdTdT-3¢(DYRK 1A# 2). Other siRNAs used were 5¢-CGUACGCGGAAUACUUCGAdTdT-3¢ (Luciferase), 5¢-CGACCUGACUGCCAAGAAAdTdT-3¢ ... pcDNA3. A portion of each cell lysate was retained as an inputsample and FLAG-immunoprecipitations were performed on the remainder. *Indicates bands resulting from IgG; arrows indicate bands of interest....
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Báo cáo Y học: Monitoring the structural consequences of Phe12 fi D-Phe and Leu15 fi Aib substitution in human/rat corticotropin releasing hormone Implications for design of CRH antagonists pdf

Báo cáo Y học: Monitoring the structural consequences of Phe12 fi D-Phe and Leu15 fi Aib substitution in human/rat corticotropin releasing hormone Implications for design of CRH antagonists pdf

... chain of the human/rat CRH analogueseems to fold to an almost linear tertiary structure with themain features being the high degree of helical character and a small bend along the principal molecular ... meaningful NOE and 32 H-bondsdistance limits) and 21 angle constraints. A family of 40energy-minimized conformers was obtained with averagermsd of 0.39 ± 0.16 A ˚ and 0.99 ± 0.13 A ˚ for backbone and ... Spyridon Papazacharias, George Pairas and Paul CordopatisDepartment of Pharmacy, University of Patras, Greece A new human/rat CRH analogue has been synthesizedusing the Fmoc/tBu solid-phase synthetic...
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Tài liệu Báo cáo khoa học: The tandemly repeated domains of a b-propeller phytase act synergistically to increase catalytic efficiency doc

Tài liệu Báo cáo khoa học: The tandemly repeated domains of a b-propeller phytase act synergistically to increase catalytic efficiency doc

... InsP6gradually into InsP5, InsP4, and the final product – Ins(2,4,6)P3 and Ins(1,3,5)P3–via two alternative pathways [14].Bacterial BPPs containing two tandemly repeateddomains (dual domains) ... can increasethe amount of available phosphate by interacting together. Additionally,fusing PhyH-DI to a single-domain phytase appears to be an efficient wayto improve the activity of the latter.IntroductionPhytate ... minimum around 255 nm and a large positive maximum around 298 nm. The structurewas intact after 12 h of incubation and failed to refoldafter 5 min of boiling.The kinetic values for PhyH and PhyH-DII...
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