... Watanabe,T.,Kobori,K.,Kiyotaka,Miyashita,Fujii,T.,Sakai,H. & Makot, Uchida and Tanaka, H. (1993) Identification of glutamic acid 204 and aspartic acid 200 in chitinase A1 of Bacilluscirculans WL-12 as essential residues for chitinase activity. ... which increases in molecular mass and DA resulted in efficient loading and minimization of therelease of entrapped lipase [30]. The observed increase in specific activity with increasing molecular ... precipitate (LMWC) obtainedafter centrifugation at 1000 g, was dialyzed against waterusing a membrane with a molecular mass cut-off of 2 kDa(Sigma Chemicals Co.) and freeze-dried.The supernatant...