... IGVL1-44*01 and IGLJ1*01. The closest germ line, diver-sity and joining segments for the VHdomain of clone 3F wereIGHV3-9*01; IGHD2-8*02; IGHJ3*02. For the VK, the germ line and the joining segments ... closestgerm line, diversity and joining segments for the VHdomain of cloneC1 were IGHV3-30*18, IGHD2-21*01 and IGHJ2*01, respectively.For the VLdomain, the germ line and the joining segments corres-ponded ... consequence of direc-ted evolution [36] or shortening of the linker peptide(unpublished results), resulted in an improvement in the stability of the single chain. The diabodies of evolved clones 6F and...