Báo cáo khoa học: Thyroid hormone induces the expression of 4-1BB and activation of caspases in a thyroid hormone receptor-dependent manner pptx
Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
... specificity and activity of ervatamins, the papain-like cysteine proteasesfrom a tropical plant, Ervatamia coronariaRaka Ghosh, Sibani Chakraborty, Chandana Chakrabarti, Jiban Kanti Dattagupta and Sampa ... ervatamin -A and ervatamin-C The modes of binding of E-64 with ervatamin -A and ervatamin-C have been analyzed and compared with the structures of other complexes of the same family.E-64 binds ... and other members of the papain family, and is made up of two domains, L and R, with a V-shaped active sitecleft at the interface of the domains (Fig. 1).Amino acid sequence of ervatamin -A and comparison...
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Báo cáo khoa học: Microcin J25 induces the opening of the mitochondrial transition pore and cytochrome c release through superoxide generation doc
... that is active against certain human pathogenssuch as Salmonella and Shigella [1], has an unusuallasso distinctive structure [2–4] and a dual mechanism of action. Microcin J25 inhibits transcription ... showing an initial rate of 7.02 4 light scattering ⁄ min · 104. The initial rate of swelling increased as a function of MccJ25 concentra-tion (inset in Fig. 2) and became saturated at about60 ... a small increase in the internal calciumconcentration, which in turn activates the uniporter of calcium, increasing even more the calcium in ux and ROS production. Both the increase of intramitochond-rial...
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Tài liệu Báo cáo khoa học: Oxygen tension regulates the expression of a group of procollagen hydroxylases docx
... time-dependent increase of the mRNA abun-dance in A7 r5 cells incubated at 1% oxygen for P4ha1 and P4ha2, starting around 4 h of hypoxia, the induction of P4ha2 mRNA being stronger than that of P4ha1 (Fig. ... 12 h of stimulation (Fig. 5A) . The stimu-latory effect of hypoxia and of deferoxamine on P4ha1 and P4ha2 mRNA and PDI mRNA was almost abrogated in Hepa1C4 cells (Fig. 5B), supporting the assumption ... mouse and rat subset of the NCBInr.fasta protein database.RNA isolationTotal RNA was extracted from freshly harvested cells and from frozen tissues according to the protocol of Chomczynski and Sacchi...
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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
... spectro-metry.SDS/PAGE was carried out in separating gels containing15% acrylamide. The following proteins were used asstandards: phosphorylase B (97 kDa), bovine serum albu-min (67 kDa), ovalbumin (45 kDa), ... [23] and Beinert [24], respectively.Kinetic constants The catalytic activity of PHR was evaluated both in the presence and in the absence of PHI.Km and kcatwere determined from Hanes–Haldane ... electron transfer toPHO; (c) PHI–PHO interaction, possibly causing a conformational change leading to the opening of the PHO active site.Materials and methodsBacterial strain The A. radioresistens...
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Tài liệu Báo cáo khoa học: Isoprenoid biosynthesis via the methylerythritol phosphate pathway Mechanistic investigations of the 1-deoxy-D-xylulose 5-phosphate reductoisomerase ppt
... byincubating the aldehyde in the presence of NADP+.Asmethylerythrose and DX, as well as the diacetate of methylerythrose and the triacetate of DX, have the sameRf, the presence of DX triacetate ... concentrations of NADP+ and MEP 5 ,the substrates of the reverse reaction, and of NADPH and DXP3, the products of the reaction, on the total amount of NADPH produced were analysed. The apparent ... to the reaction medium did not induce anyadditional absorbance increase. Ceasing NADPH forma-tion was not due to the inactivation of the enzyme, butrather to the fact that the equilibrium had...
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Báo cáo khoa học: New insights into the P-glycoprotein-mediated effluxes of rhodamines doc
... that there was noaccumulation of TMR inside the cells and therefore that the potentials were eliminated.Table 1. Kinetic parameters for rhodamine and anthracycline derivatives. k a , the rate ... P-gp-associated efflux of rhodaminewas calculated at the steady-state, taking into account the following points: (a) the diffusion (in ux and efflux) of rhodamine through the membrane is passive, ... substratetransport, is a powerful approach to enhancing ourunderstanding of their function and mechanism. In thispaper, we present data that further characterize the transport of several rhodamine analogues....
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Báo cáo khoa học: Peptides that bind the HIV-1 integrase and modulate its enzymatic activity – kinetic studies and mode of action pptx
... sameslope was maintained (Fig. 2A C and Table S2). Theseresults further suggest that these peptides enhance the dissociation of IN and its DNA substrate or inhibit the initial IN DNA binding, an observation ... mgÆL)1purified IN. The reaction was initiated by the addition of 400 nm of the 3¢-processing substrate (5¢-[FAM]-ACTGCTAGAGATTTTCCACGTGGAAAATCTCTAGCAGT-[DABCYL]-3¢) or control substrate (5¢-[FAM]-TGCTAGAGATTTTCCACGTGGAAAATCTCTAGCA-[DABCYL]-3¢). ... demonstrating that the LEDGF 361–370 unique binding site on IN participates in the IN tetramerization interface. (C) INSuniquely binds the IN sequence that participates in the binding of the drug raltegravir...
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Báo cáo khoa học: Heme binding to the second, lower-affinity site of the global iron regulator Irr from Rhizobium leguminosarum promotes oligomerization potx
... Brucella, Rickettsia) and plants (Agrobacterium), symbionts (the N2-fixing rhizo-bia), many of the most abundant bacteria in the oceans (the Roseobacters and the SAR11 clade, includ-ing Pelagibacter) ... remaining after the addition of heme (Fig. 4A) . A simple analysis of absorbance changes indicated that oligomerization of H4 6A and H6 6A IrrRl in the presence of heme wasapproximately 60% of ... members of the Rhizobiaceae family, including the closely related Sinorhizobium and Agrobacterium,as well as in strains of Mesorhizobium (in the familyPhyllobacteriaceae). Interestingly, all of these...
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Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): reactivity and structure of metal–thiolate clusters* doc
... domains (a b-domain for the N-terminal cluster and an a- domain for the C-terminal cluster). In the absence of metals, apo-thionein (apo-T) is predominantly unstruc-tured and only upon metal ... cysteines and the absence of His and aromatic amino acids. MT3 contains 68amino acids with 70% sequence identity to the MT1 and MT2 (MT1 ⁄ 2) isoforms. The MT3 sequencecontains two inserts: an acidic ... speculate that higher structural dynamics shouldalso result in a faster metal transfer from and to MT3 and hence is in line with an involvement in the traffick-ing of Zn(II) in particular in zinc-containing...
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Báo cáo khoa học: Enzymatic toxins from snake venom: structural characterization and mechanism of catalysis ppt
... catalytic subunit has similarlight and heavy chains to trocarin D. The non-enzy-matic subunit has a heavy chain (consisting of A1 and A2 domains) and a light chain (consisting of A3 , C1 and ... Typical ADAMs are type-1integral membrane proteins and have an epidermal-growth-factor-like domain, a transmembrane domain and a cytoplasmic domain, in addition to the metallo-proteinase ⁄ disintegrin ... products of the processing of P-IIIb SVMPs. Jararhagin-C, catro-collastain-C and leberagin-C, which are D ⁄ C-domain-containing fragments isolated from Bothrops jararaca,C. atrox and Macrovipera...
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