Báo cáo khoa học: Amino acid biosynthesis and metabolic ﬂux proﬁling of Pichia pastoris ppt

... Amino acid biosynthesis and metabolic flux profiling of Pichia pastoris Aina Sola`1,2, Hannu Maaheimo2,3, Katri Ylo¨ nen3, Pau Ferrer1 and Thomas Szyperski21Department of Chemical ... study of amino acid biosynthesis and central carbon metabolism of the yeastP. pastoris. In the framework of this study, we haveestablished the BDF13C-labeling approach of proteinogenic amino acids ... Germany) and dried through a silica gelÓ FEBS 2004 Metabolic flux profiling of Pichia pastoris (Eur. J. Biochem. 271) 2463column. Concentrations of oxygen and carbon dioxide inthe exhaust gas of bioreactor...

Báo cáo khoa học: Proteoglycans in health and disease: the multiple roles of syndecan shedding ppt

... residues and have a common stemtetrasaccharide of xylose (xyl), two galactose units (gal) and a glucuronic acid residue (GlcA). The repeating disaccharide of HS is N-acetylglu-cosamine and uronic acid, ... 6-0NNN6-0NNFig. 1. Schematic of the four vertebrate syndecans. Syndecans-1 and -3 core proteins are larger than those of syndecan-2 and -4, and canbear both heparan and chondroitin sulfate chains. ... SA, Copeland RA,Decicco CP, Wynn R et al. (1999) Puriﬁcation and cloning of aggrecanase-1: a member of the ADAMTSfamily of proteins. Science284, 1664–1666.53 Echtermeyer F, Bertrand J, Dreier...

Báo cáo khoa học: Amino acid limitation regulates the expression of genes involved in several speciﬁc biological processes through GCN2-dependent and GCN2-independent pathways ppt

... Usingtranscriptional proﬁling, the objective of this work wasto gain a better understanding of the amino acid con-trol of gene expression. As our aim was to study theeffects of short-term amino acid starvation, ... recognition of protein quality actsthrough the sensing of free circulating amino acidsresulting from the intestinal digestion of proteins [4,5].Another example of the detection of the lack of an amino ... avoid the long-term and secondary effects of amino acid starvation.Our data demonstrate that a 6 h amino acid starva-tion regulates the expression of a speciﬁc set of genes: of the 25 000 genes...

Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural speciﬁcity variants doc

... thatrequire tRNA for amino acid activation, the potentialfor misrecognition of related amino acids has beeninvestigated [9–13] and modulated by amino acid replacements and active site redesign ... stability of the canavany-lated species.The role of tRNA as a cofactor for aminoacylationin those aminoacyl-tRNA synthetases that requiretRNA for amino acid activation is well documented[9], and ... Germany).AminoacylationAminoacylation was performed at 30 °C in a volume of 50 lL containing 50 mm Hepes ⁄ KOH pH 7.5, 10 mmMgCl2,4mm ATP and the appropriate amount of [14C]- amino acid, tRNA and arginyl-tRNA...

Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx

... (Q9BQS6) and ODFP (Q14990).Table 1. Frequency of amino acid residues in the flexible region of the C-terminal extensions of human and murine sHsps. Residues presentin the flexible region of the ... of wild-type and mutant Hsp25The binding of ANS and other hydrophobic probes to aprotein enables the comparative determination of theTable 2. Summary of changes in structure and function of ... shown), and is a predominant residue inthe flexible regions of both human and murine sHsps.The majority of residues present in the flexible regions of the extensions (71% and 74% for human and mur-ine,...

Báo cáo khoa học: Amino acids Thr56 and Thr58 are not essential for elongation factor 2 function in yeast potx

... [2–4].Yeast eEF2 is a protein of 842 amino acids [5]. Theprotein is evolutionary conserved and the amino acid sequence is 66% identical and 85% homologous to thesequence of human eEF2 [5]. eEF2 is ... forms of eEF2.Fig. 4. Comparison of the amino acid context surrounding the puta-tive phosphorylation site in eEF2 from various fungi, plants and metazoans. The position of threonines T56 and ... function of speciﬁc amino acids in bac-terial EFG [26–29]. To date, there are only a fewreports in which this technique has been used toacquire information on the importance of speciﬁc amino acids...

Báo cáo khoa học: Amino acid residues on the surface of soybean 4-kDa peptide involved in the interaction with its binding protein potx

... equal volumes of a 1–10 lMsolution of the digests and a saturated solution of a-cyano-4-hydroxycinnamic acid as a matrix in 50% CH3CN with 0.1% triﬂuoroacetic acid. Four microlitres of this mixture ... Phe31 and Ile33) play acritical role in binding to the 43-kDa protein.Role of amino acids in the 4-kDa peptideBy alanine-scanning mutagenesis of the 4-kDa peptide, weidentiﬁed that 13 amino acids ... hydrophobic and two arearomatic. The higher number obtained of aromatic and hydrophobic residues emphasized the importance of these amino acids in the interaction between the 4-kDa peptide and the...

Tài liệu Báo cáo khoa học: Hypothalamic malonyl-CoA and CPT1c in the treatment of obesity pptx

... user of long-chain fattyacids making a brain-specific isoform intriguing.CPT1c was identified and cloned from in silicosequences as a highly homologous member of theCPT1 class of enzymes and ... fluctuations in the levels of blood glucose [13], fattyacids [12,14,15] and certain amino acids [16].A linkage between fatty acid synthesis in the CNS and feeding behavior was uncovered with the findingthat ... lyase producing acetyl-CoA – the sole precursor of fatty acids. The initial and committed step of de novofatty acid synthesis is the carboxylation of acetyl-CoAto form malonyl-CoA, catalyzed by...

Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc

... 6. Control of the period and amplitude of Src oscillations bythe activities of the activatory phosphatase RPTP and inhibitorykinase Csk. Dependence of the oscillation amplitude (A) and period(B) ... coexistence of oscillations (limit cycle) and a stablesteady state implies subcritical Hopf bifurcation and the appearance of an unstable limit cycle. The unstable and stable limit cycles collide and ... large activities of Csk and RPTP, demonstrating robustness of thebistable behavior.Oscillations occurring within regions 3 and 4 corre-spond to lower concentrations of active Csk and RPTPthan...

Tài liệu Báo cáo khoa học: Second messenger function and the structure–activity relationship of cyclic adenosine diphosphoribose (cADPR) doc

... function and the structure–activityrelationship of cyclic adenosine diphosphoribose (cADPR)Andreas H. GuseUniversity Medical Center Hamburg-Eppendorf, Center of Experimental Medicine, Institute of ... the anomeric carbon of the ribose,which in the precursor NAD is linked to nicotinamide, and the amino ⁄ imino group at C6 of the adeninemoiety [2]. Spectroscopic data [3] and ﬁnally a crystalstructure ... foundin various cell types, tissues and organisms. Receptor-mediated formation of cADPR may proceed via transmembrane shuttling of the substrateNAD and involvement of the ectoenzyme CD38, or via...

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