Báo cáo khoa học: Anti- and pro-oxidant effects of quercetin in copper-induced low density lipoprotein oxidation Quercetin as an effective antioxidant against pro-oxidant effects of urate potx

Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt

... searches of b-amino acids wereinitially carried out at intervals of 30° for each torsion angle/, h,andw and were subsequently reﬁned by varying /, wangles in intervals of 10° and setting h angle ... Torrens, Y., Beaujouan, J C. & Glowinski, J. (1993)Importance of the leucine side-chain to the spasmogenic activity and binding of substance P analogues. Int. J. Peptide Protein Res.42, 270–277.43. ... Parameters of SP and SP analogues.Ó FEBS 2003 Eﬀects of b-amino acid insertion in Substance P (Eur. J. Biochem. 270) 949Structural and biological effects of a b2-orb3-amino acid insertion in a...

Báo cáo khoa học: Anti- and pro-oxidant effects of quercetin in copper-induced low density lipoprotein oxidation Quercetin as an effective antioxidant against pro-oxidant effects of urate potx

... Anti- and pro-oxidant effects of quercetin in copper-induced low density lipoprotein oxidation Quercetin as an effective antioxidant against pro-oxidant effects of urate Paulo Filipe1,2, ... that•UH–wouldalsobeinvolved in the pro-oxidant action of urate. Interestingly,at high Cu2+concentration, in the presence of 0.5 lM quercetin and 10 lM urate, the LDL oxidation measured in terms of MDA and ... that in the absence of quercetin the pro-oxidant activity of urate masks its antioxidant activity as mentioned above, whereas, in the presence of quercetin, the pro-oxidant activity of urate...

Tài liệu Báo cáo khoa học: Temperature and phosphate effects on allosteric phenomena of phosphofructokinase from a hibernating ground squirrel (Spermophilus lateralis) pptx

... area of the White Moun-tains of California. Details of animal holding, feeding and hibernation were described in [15]. All possible measureswere taken to minimise pain and discomfort during animaleuthanasia ... stability of plasma glucose levels [1,3,4]. In hibernators, the aci-dotic conditions found in muscle during hibernationcan lead to an increase in histidine protonation and these effects may be translated ... Kavalue of 2.0 ± 0.2 mm. However, inorganicphosphate was unable to elicit an activation of PFKactivity at 5 °C; in fact, the addition of inorganic phos-phate resulted in an inhibition of PFK...

Báo cáo khoa học: Spectroscopic and kinetic properties of the horseradish peroxidase mutant T171S Evidence for selective effects on the reduced state of the enzyme potx

... UKHorseradish peroxidase (HRPC) is a member of classIII of the plant peroxidase superfamily and is cap-able of utilizing hydrogen peroxide to oxidize a widerange of phenols, anilines and other synthetic ... features can also exert an important in uence in determining the electronic structure of the haem pocket. Toextend our understanding of the signiﬁcance of proximal characteristics in regulating haem ... are assigned by anal-ogy to myoglobin and cytochrome c peroxidase (CCP)[22,23] to out -of- plane modes of the porphyrin ringitself together with the bending modes of the propionyl and vinyl...

Báo cáo khoa học: Anti-HIV-1 activity of 3-deaza-adenosine analogs Inhibition of S-adenosylhomocysteine hydrolase and nucleotide congeners pot

... by:hEINTi¼EmincomplexþNcomplexkBT2À EminproteinÀNproteinkBT2À EminligandÀNligandkBT2ð2Þ and as Ncomplex¼ Nprotein+Nligand,hEINTi¼DE ¼ EmincomplexÀ EminproteinÀ ... reduction in the Kifor theinhibition of AdoHcy hydrolase and a corresponding25- and 13-fold decrease in the inhibition of HIV-1 A012 and A018 p24 antigen in PBMC, respectively. These resultsindicated ... Helland, S. & Ueland, P.M. (1981) Interaction of 9-b-D-arabino-furanosyladenine, 9-b-D- arabinofuranosyladenine 5¢-monophos-phate, and 9-b-D-arabinofuranosyladenine 5¢-triphosphate...

Báo cáo khoa học: Cholinoceptive and cholinergic properties of cardiomyocytes involving an ampliﬁcation mechanism for vagal efferent effects in sparsely innervated ventricular myocardium ppt

... activities of donepezil and other cholinesterase inhibitors on acetylcholinesterase and butyrylcholinesterase in vitro. Methods Find ExpClin Pharmacol 22, 609–613.28 Papandreou I, Cairns RA, Fontana ... JapanIntroductionOur previous studies showed that an oral low dose of donepezil, an acetylcholinesterase inhibitor and anti- Alzheimer’s disease drug, prevented ventricular remod-eling and ... staining technique. This studywas supported by a grant -in- aid for scientiﬁc research(19590251, 19659355) from the Ministry of Education,Science, Sports, and Culture of Japan, and by Healthand...

Báo cáo khoa học: Temperature and salts effects on the peptidase activities of the recombinant metallooligopeptidases neurolysin and thimet oligopeptidase pdf

... of neu-rolysin and TOP was reported using internally quenchedfluorescent (IQF) peptides derived from bradykinin [5] and neurotensin [6]. An outstanding feature of the hydrolyticactivities of ... unspecificcleavage of other proteins and it is of particular relevancefor TOP and neurolysin as they are not expressed as inactiveprecursors and they are present in high amount as solubleenzymes in cytosol. ... determined in the presence of 2MNaCl. In this condition, the Eyring plots obtained forTOP and neurolysin gave two linear fittings, above and below % 22 °C, which contrast with linear plots obtained...

Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt

... inducer of neovascularization, and plays an important role in tumor growth [5]. Eosinophil-derived neurotoxin(RNase 2) and eosinophil cationic protein (RNase 3)have antibacterial and antiviral activities. ... regarding a new class of surrogates for the phosphoryl group.Results and DiscussionSulfonimides as inhibitors of RNase AWe began by determining the ability of three backboneanalogs of RNA to inhibit ... and structural analyses of N-acylsulfonamide-linked dinucleoside inhibitors of RNase ANethaji Thiyagarajan1, Bryan D. Smith2,*, Ronald T. Raines2,3 and K. Ravi Acharya11 Department of...

Tài liệu Báo cáo khoa học: Active and regulatory sites of cytosolic 5¢-nucleotidase doc

... activity and is measured at saturating concentrations of IMP and sub-saturating concentrations of inosine. The K50for ATP wasmeasured as phosphotransferase activity, while for Mg2+, it was measured ... behav-iour of recombinant wild-type enzyme in the presence of ATP and Mg2+ as an activator and Pi as an inhibitor.Figure 5 shows that the enzyme exists and functions as atetramer irrespectively of ... dramaticincrease in the ratio of nucleotidase to phosphotrans-ferase activity. The afﬁnity for the substrates inosine and IMP remains unaltered, and the effect of theenzyme activators ATP and Mg2+is...

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... with guanine and hypoxanthinebases, respectively, indicating that Asp137 functions as a catalytic base [12]. However, a tight binding of N7 of the purine base and Asp137 was observed in HPRT in ... PRTFDC1 and hypoxanthine. (B) HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine.Table 2. Km and Vmaxvalues for Hx and G in the presence of 1 mM PRPP, determined using the DE-81 ... activity of PRTFDC1towards hypoxanthine and guanine is only 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metabolism remainsunclear and it is uncertain...

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