Báo cáo khoa học: Crystal structure of human cystatin C stabilized against amyloid formation pdf

... of papain-like cysteine proteases.AbbreviationsAS, appending structure; HCC, human cystatin C; HCCAA, hereditary cystatin C amyloid angiopathy; HCC-stab, monomer -stabilized human cystatin C; ... models of type 2 cystatins, such as chicken cystatin (PDB code:1CEW), cystatin D (PDB code: 1RN7), or cystatin F(PDB code: 2CH9). In addition, it is possible tocompare the monomeric fold of HCC-stab1 ... Expression of a selenomethionylderivative and preliminary crystallographic studies of human cystatin C. Acta Crystallogr D Biol Crystallogr55, 1939–1942. Structure of monomeric cystatin C R. Kolodziejczyk...

Báo cáo khoa học: Crystal structure of the halotolerant c-glutamyltranspeptidase from Bacillus subtilis in complex with glutamate reveals a unique architecture of the solvent-exposed catalytic pocket docx

... LaJolla, CA, USA) with forward primer 5¢-GAAACGATGCATTTGTCCTATGCCGACCGTGCGTC-3¢ and reverseprimer 5¢-GACGCACGGTCGGCATAGGACAAATGCATCGTTTC-3¢. The sequence of the second PCR product wasalso conﬁrmed. ... signiﬁcance of the lid-loop in GGTcatalysis.During the preparation of this article, the crystal structures of Bacillus anthracis CapD, a GGT-relatedenzyme, in the absence and presence of a ... autocatalytic cleavage of the inactive pre-cursor protein ( 60 kDa) [7]. Crystallographic studies of GGTs from Escherichia coli and Helicobacter pylorihave revealed the detailed molecular mechanisms...

Báo cáo khoa học: Solution structure of human proinsulin C-peptide ppt

... structure of proinsulin C- peptide in aqueous solution and in thepresence of lipid vesicles. Cell Mol Life Sci 57, 337–342.27 Xie L & Tsou C- L (1993) Comparison of secondarystructures of ... folded con-formations, it can be used to predict the secondary- structure content of the time or ensemble average. Thedifferences DdHa of the measured chemical shifts fromrandom coil conformation ... these conditions. The chemical shifts indicatethat with increasing 2,2,2-triﬂuoroethanol concentra-tion the content of local secondary structures increases.The best-resolved NOESY spectra were...

Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... pocket are completelydifferent, possibly accounting for the clear differencesin reaction kinetics between basidiomycete laccasesand asco-laccases.DimerizationIn the TaLcc1 crystal structure, ... basidio-mycete laccases, such as T. versicolor, L. tigrinus and C. cinereus laccases, have Asp residues here. In the crystal structure of the basiodiomycete T. hirsuta lac-case (PDB code3FPX), the corresponding ... (asco-laccase) MaL, thus giv-ing a comprehensive view of the structure and function of asco-laccases.Results and DiscussionOverall structure The crystal structure of TaLcc1 was solved to 2.5-A˚resolution...

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... peptide complex (observed structure factorsFpepo) with an apo structure of importin-a (70–529)(observed structure factors Fapoo). This structure is describedin ref. [42]. The structure factors ... integration of macromoleculardiffraction data. Acta Crystallogr D: Biol Crystallogr62, 48–57.36 Collaborative Computational Project Number 4 (1994)The CCP4 suite: programs for protein crystallography.Acta ... demonstrated an interactionbetween importin-a and the chloride intracellular chan-nel (CLIC) protein, CLIC4 [2,3].The structure of a soluble form of CLIC4 showsthat it adopts the canonical glutathione...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... metalcofactor, respectively. SODs are grouped into fourclasses according to their metal cofactors: copper andzinc-containing SOD (Cu,Zn-SOD), iron-containingSOD (Fe-SOD), manganese-containing ... ﬁrst time, we describe the crystal structure of ApeSOD. In particular, we focuson the coordination of the metal cofactor in the activesite as well as the changes it experiences in response todifferent ... from the aerobichyperthermophilic archaeon Aeropyrum pernix K1.Acta Crystallogr Sect F: Struct Biol Cryst Commun61, 323–325.32 Edelhoch H (1967) Spectroscopic determination of tryptophan and...

Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

... the structure of PhyK and four in PhyK H25A),these structural differences are not caused by crystal lattice contacts.Another conformational change in AppA involvesGlu219. The side chain of ... LaCount MW & Lebioda L (2003) Crystal structures of human prostatic acid phosphatase in com-plex with a phosphate ion and a-benzylaminobenzyl-phosphonic acid update the mechanistic picture ... U & Umbach P(2003) Facilities and methods for the high-throughput crystal structural analysis of human proteins. Acc ChemRes 36, 157–163.K. Bo¨hm et al. Crystal structure of Klebsiella...

Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

... regulators of the proteolyticactivity of DESC1. The reactive site loop sequences of PAI-1 and PCI fit to the active site geometry of human DESC1, without matching the optimal dockingsequence of DESC1. ... mainchain of the catalytic domain (B-chain) of DESC1 is welldefined. Only a few side chains on the surface of the mole-cule are partially undefined in the electron density. Theoccupancy of all ... enteropeptidase,Scheme 1. Domain organization of human DESC1.O. J. P. Kyrieleis et al. Crystal structure of the catalytic domain of DESC1FEBS Journal 274 (2007) 2148–2160 ª 2007 Max-Planck-Insitute of Biochemistry...

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... Deacetylase activity of the BcZBP protein on N-acetylchi-tooligosaccharides.Substrate Deacetylation (%)GlcNAc 100(GlcNAc)289.82(GlcNAc)310.51(GlcNAc)45.51(GlcNAc)512.19(GlcNAc)63.93Fig. ... Model of the BcZBP–GlcNAc com-plex. Stereoview of the energy minimizedputative BcZBP–GlcNAc complex. A slicethrough the active site cavity shows thequality of ﬁt of the N-acetylglucosaminemolecule ... (2004) Concerted action of diacetylchitobiose deacety-lase and exo-b-D-glucosaminidase in a novel chitinolyticpathway in the hyperthermophilic archaeon Thermo-coccus kodakaraensis KOD1. J Biol Chem...

Tài liệu Báo cáo khoa học: Crystal structure of a subtilisin-like serine proteinase from a psychrotrophic Vibrio species reveals structural aspects of cold adaptation docx

... pooled and concentrated in centrifugal concentrators(Centricon and Minicon from Millipore) for crystallizationtrials.Crystallization and data collectionRecombinant Vibrio proteinase was crystallized ... reﬁnement of protein molecules. Acta Crystallogr Sect D 49, 129–147.78 Kleywegt GJ (1996) Use of non-crystallographic sym-metry in protein structure reﬁnement. Acta CrystallogrSect D 52, 842–857.79 ... known structure of proteinase K (PDB accession number, 1IC6) as asearch model. The crystallized 30 kDa catalytic domain of Vibrio proteinase encompasses amino acids 140–420 of the 530 amino acid...

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