Báo cáo khoa học: Crystal structure of the halotolerant c-glutamyltranspeptidase from Bacillus subtilis in complex with glutamate reveals a unique architecture of the solvent-exposed catalytic pocket docx
Báo cáo khoa học: Crystal structure of the halotolerant c-glutamyltranspeptidase from Bacillus subtilis in complex with glutamate reveals a unique architecture of the solvent-exposed catalytic pocket docx
... deeplygrooved catalytic pockets in the asymmetric unit, and the glutamate- binding modes are identical to eachother (Fig. 2A) . The a- carboxyl and a- amino groups of the bound glutamate are at the bottom of ... report the crystal structure of B. subtilis GGT in complex with glutamate, a product of the enzymatic reaction, at 1.95 A ˚resolution, revealing the unique structure of the catalytic pocket. We also ... the plasmid pCY167 (Suzuki H & Yamada C, Unpublished),using forward primer 5¢-CATATGGATGAGTACAAACAAGTAGATG-3¢ and reverse primer 5¢-GGATCCTCGAGCTCATTTACGTTTTAAATTAATGCCGAT-3¢ (underlin-ed...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... 13% of the accessible surface area of each dimer. Neighboring tet-ramers came into loose contact with each other in the crystal packing. Similar molecular arrangements havebeen found in the crystal ... bound to the metal, in the company of a water oxygen, from the apical positions. The manga-nese was only 0.06 A ˚out of the equatorial plane(Table 3). The angles around the metal cofactor sug-gested ... UegakiK, Hagihara Y, Ataka M & Ishikawa K (2005)Crystallization and preliminary X-ray diffractionanalysis of thioredoxin peroxidase from the aerobichyperthermophilic archaeon Aeropyrum...
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Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx
... medi-ate interaction of the two b-barrels, formation of the catalytic machinery and structures required for binding of the main chain of the substrate peptide and properpositioning of the scissile ... mainchain of the catalytic domain (B-chain) of DESC1 is welldefined. Only a few side chains on the surface of the mole-cule are partially undefined in the electron density. The occupancy of all ... analysis of DESC1 suggests a possible rigiddomain association between the N-terminal SEAdomain and the back site of the proteinase domain.This interaction would fix the SEA domain in a loca-tion...
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Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc
... concentra-tion justifies the presence of acetate in the crystal structure. The binding of acetate in the active site is a further indication that the enzyme may be involved in deacetylation because acetate ... present the crystal structure of BcZBP at 1.8 A ˚resolution. The N-terminal part of the 234amino acid protein adopts a Rossmann fold whereas the C-terminal partconsists of two b-strands and two a- helices. ... Ivanova N, Sorokin A, Anderson I, Galleron N,Candelon B, Kapatral V, Bhattacharyya A, Reznik G,Mikhailova N, Lapidus A et al. (2003) Genomesequence of Bacillus cereus and comparative analysiswith...
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Báo cáo khoa học: Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator Tris pdf
... l-glutamine, with that of Erwinia carotovora asparaginase, which has substrates other thanl-glutamine, shows that Mglu has a larger substrate-binding pocket thatprevents the binding of l-asparagine ... the structure of E. carotovoraasparaginase in complex with l -glutamate (PDB 2hln)[18]. Mglu binds l -glutamate in an extended form(Fig. 7A) with a contact area of 288 A ˚2. Asparaginasebinds ... gluta-minase to gain insight into the mechanism of salt toler-ance. The liganded (TG) structure was also compared with that of Erwinia carotovora asparaginase, whichshows a broader substrate...
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Báo cáo khoa học: Crystal structure of the parasite inhibitor chagasin in complex with papain allows identification of structural requirements for broad reactivity and specificity determinants for target proteases pptx
... of the interactions of papain with loop L6 of chagasin and loop L2 of cysta-tin B. (C) Zoom -in view of the interactions of papain with loop L4 of chagasin and the N-terminal segment of cystatin ... Od1atom of N64e forms a water-mediated contact with the main-chain N atom of G68, and the main-chainnitrogen of G66 of chagasin forms a water-mediated A BCDFig. 2. Interactions of chagasin and ... of chagasin in complexes with cathepsin L, cathepsin B and falci-pain allowed the creation of a consensus map of the structural features thatare important for efficient inhibition of papain-like...
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Báo cáo khoa học: Crystal structure of the tetrameric inositol 1-phosphate phosphatase (TM1415) from the hyperthermophile, Thermotoga maritima docx
... quaternary organization maybe critical in creating regulatory abilities. Crystal packingBoth crystal forms of TM1415 have a tetramer present in the asymmetric unit with similar packing of individ-ual ... on the structure of the eukaryotic FBPase takes a 30° rotation in the twist direction of the archaealenzyme. In comparing the dimer orientation of allthese IMPases, we see that the human IMPase ... containing the act-ive site. The lower domain is reminiscent of the AMP-binding domain of eukaryotic FBPases (supplementaryFig. S2). The active site is located at the bulge of the C-terminal...
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Báo cáo khóa học: Crystal structure of the chi:psi subassembly of the Escherichia coli DNA polymerase clamp-loader complex doc
... by a core DNA polymerase assembly that is attached to a slidingclamp. Although a single sliding clamp may remainattached to the polymerase during replication of the leadingstrand, each Okazaki ... nuclear antigen (PCNA) in eukaryotes and archaebacteria] that tether the DNApolymerase to DNA [2]. The interaction between the DNA polymerase and the sliding clamp enables the activesite of the ... Monocliniccrystals, containing two v:w complexes in the asymmetricunit, diffract X-rays to % 2A ˚Bragg spacings on a rotatinganode X-ray source. The crystal structure of the v:w complex was...
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Báo cáo khoa học: Crystal structure of the soluble form of the redox-regulated chloride ion channel protein CLIC4 doc
... were used: the Ramachandran distance and a real-space distance. The Ramachandran distance, D, wascomputed by comparing the Ramachandran plots forCLIC1 and CLIC4(ext) using the equation:D ¼fðUCLIC4À ... coordi-nates and structure factors have been deposited in the Protein Data Bank (accession code 2AHE).Ramachandran and real-space distancesTo locate structural changes in an unbiased fashion, twomeasures ... sequences of the invertebrate CLICs from Drosophila melanogaster (AAF48326), Anopheles gam-bia (EAA45365) and Schistosoma japonica (AAP06293),however, it may be present in the sequence of the Caenorhabditis...
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Báo cáo khoa học: Crystal structure of the second PDZ domain of SAP97 in complex with a GluR-A C-terminal peptide ppt
... between bB and aB,which may arise from binding of the C-terminus of a neighboring PDZ domain in the crystal. We thereforeused chain A, which does not contain anything in the ligand binding site, ... typical architecture for PDZ domains. In the structure of the peptide complex, only the last four C-terminal residues of the GluR- A are visible, and align as an antiparallel b-strand in the binding ... C-terminal peptide and SAP97PDZ2[19]. In an attempt to understand the structural basis of GluR -A SAP97 interaction, we have determined the crystal structure of SAP97PDZ2 in the presence andabsence...
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