Báo cáo khoa học: Expression of CYP2E1 increases oxidative stress and induces apoptosis of cardiomyocytes in transgenic mice pdf

Báo cáo khoa học: The essential tyrosine-containing loop conformation and the role of the C-terminal multi-helix region in eukaryotic phenylalanine ammonia-lyases docx

... models. In these 40-A˚sphere portions, calculations were performed onthe following selection of residues: 26–121 and 325–343(chain A), 266–295 (chain B) and 385–399 (chain C) in SmPALout ⁄ in and ... decrease in intracellular cinnamate levels, suggesting feedback inhi-bition (i.e. autoregulation) of PAL at a certain level of endogenous cinnamate [16].PAL in higher plants is coded by a family of ... taxolbiosynthesis in Taxus chinensis [27] or T. cuspidata[28] and tyrosine 2,3-aminomutase (TAM), which isinvolved in biosynthesis of a natural product havingpotent antimicrobial and antitumor activity in...

Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx

... Apo-ACP (O) and holo-ACP (h) in each fraction was quantiﬁed by measuring the intensity of the silver-stained protein bands usingQUANTITY ONE software and plotted against the fraction number (AU, ... divided into four parts (I, II, III and IV):I, interactions with amino acids 30–33; II, interactions with amino acids 33–34; III, interactions with amino acids 35–37; IV, interactions withamino ... thrombin cleavage site was engineered in the pET28a vector immediately after the N-terminal His-tag.The N-terminus His-tag of ACP was cleaved by the addition of 1 U of thrombin from bovine plasma...

Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt

... transplantation in rat models of myocardial infarction has anti -in ammatory effects,decreasing protein production and gene expression forIL-1b and TNF-a [11]. To address these paradoxes of both pro- and ... 11-7082 inhibitedhypoxia ⁄ SD-induced NF-jBp65 translocation, withsubstantial levels of NF-jBp65 staining remaining in the cytoplasm of most cells. These results demonstratethat hypoxia ⁄ SD induces ... kinase 1 ⁄ 2 (ERK1 ⁄ 2) and p38 mitogen-activated protein kinase (p38) signal-ling pathways play important roles in hypoxia ⁄SD-induced apoptosis of MSCs [20,21] and may alsoaffect IL-1b and...

Tài liệu Báo cáo khoa học: Expression of poly(A)-binding protein is upregulated during recovery from heat shock in HeLa cells doc

... lg of plasmid DNAusing 10 lg of lipofectamine (Invitrogen, Burlington,Canada), according to protocols supplied by the manufac-turer. In short, the DNA was mixed with lipofectamine and PABP expression ... basis of ligand recognition by PABC, a highly speciﬁc peptide-binding domain found in poly(A)-binding protein and aHECT ubiquitin ligase. EMBO J 23, 272–281.9 Craig A (1998) Interaction of polyadenylate-bindingprotein ... step in protein syn-thesis, and is usually regulated by several importantinitiation factors, including poly(A)-binding protein(PABP) 1 and eukaryotic initiation factors (eIFs) 4E,4G, 2a, and...

Tài liệu Báo cáo khoa học: Expression and function of Noxo1c, an alternative splicing form of the NADPH oxidase organizer 1 doc

... (PX)domain in the N-terminus. PX domains occur in avariety of proteins involved in cell signaling, mem-brane trafficking, and polarity establishment, and func-tion as phosphoinositide-binding modules ... in the testis and fetal brain. The Noxo1c protein contains anadditional five amino acids in the N-terminal PX domain, a phosphoinosi-tide-binding module; the domain plays an essential role in ... phosphoinositide binding[31]. The five-amino-acid insertion in the loop of Noxo1 might alter the configuration of the phospho-inositide-binding region, affecting the affinity for Expression and function...

Tài liệu Báo cáo khoa học: Expression of an a-1,3-glucanase during mycoparasitic interaction of Trichoderma asperellum docx

... chloride and water, and transferred to a new flask containing Mandel’sminimum medium supplemented with different carbonsources (replacement medium). In the purification of AGN13.2, 0.5% of B. cinerea ... properties of AGN13.2Physicochemical and kinetic parameters of AGN13.2are summarized in Table 1. The release of reducingsugars was detected only when using mutan as sub-strate. The main product ... due to its obliged linearcharacter.Protein sequenceThe N-terminal peptide of the purified protein was sub-jected to sequencing and an eight amino acid sequencewas obtained. These residues...

Tài liệu Báo cáo khoa học: Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions doc

... reinhardtii HydA1 and HydA2) upstream of the H-cluster motif 1 and (b) asecond amino acid insertion (16 residues in C. fusca,16 residues in S. obliquus HydA1 and HydA2, 45 residues in C. reinhardtii ... differences in the regulation of expression of the two genes.Gene expression during anaerobic inductionThe coexpression of the HydA1 and HydA2 transcripts wasstudied by Northern blot analysis. In Fig. ... the HydA1 and HydA2 genes were cloned intothe corresponding site of the pET-16b expression vector(Novagen, Madison, WI, USA) producing pET-HydA1 and pET-HydA2. The inserts of pET-HydA1 and pET-HydA2...

Báo cáo khoa học: Expression of cholinesterases in human kidney and its variation in renal cell carcinoma types pdf

... erythrocytes in the extent of binding with thelectins concanavalin A, Lens culinaris agglutinin(LCA), and Ricinus communis agglutinin (RCA)(Fig. 3), ruled out the blood origin and supported therenal ... proposal is in agreementwith the cytochemical staining of acetylcholinesterase in the capsule of Bowman [41] and of acetylcholines-terase and butyrylcholinesterase in the glomerulus and the tubule, ... pathological signs of Alzheimer’s disease in the brain in vivo.Neurobiol Aging doi:10.1016/j.neurobiolaging.2010.04.028.26 Vidal CJ (2005) Expression of cholinesterases in brain and non-brain tumours....

Báo cáo khoa học: Acidic extracellular pH increases calcium inﬂux-triggered phospholipase D activity along with acidic sphingomyelinase activation to induce matrix metalloproteinase-9 expression in mouse metastatic melanoma pot

... (2002) Solu-ble Fas ligand activates the sphingomyelin pathway and induces apoptosis in luteal steroidogenic cells indepen-dently of stress- activated p38 (MAPK). Endocrinology143, 4350–4357.23 ... G (1992) In vitro and in vivo enhancement of ricin-A chain immu-notoxin activity by novel indolizine calcium channelblockers: delayed intracellular degradation linked to lipi-dosis induction. ... pH and that the speciﬁc acidic sphingomyelinase inhibitors(perhexiline and desipramine) and siRNA targeting aSMase ⁄ smpd1 couldinhibit acidic extracellular pH-induced matrix metalloproteinase-9...

Báo cáo khoa học: Expression and physiological role of CCN4⁄Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes potx

... reported in a few in human gastrointestinal cancer tissues, thus sug-gesting the involvement of the variant in malignant phe-notypes of certain cancers [17–20]. However, because of its initial ... roles of WISP1v in normal cells. In par-Fig. 6. Immunohistochemical staining of CCN4 ⁄ WISP1 and WISP1v in the developing tibia of a mouse embryo. The tibia at embryonicday of 17 was probed using ... constitutive expression of the full-length and thedifferentiation stage-dependent expression of WISP1v in vitro.Effect of overexpression of CCN4/WISP1 and itsvariants on the phenotypes of humanchondrocytic...

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