Báo cáo khoa học: Selective modulation of protein C afﬁnity for EPCR and phospholipids by Gla domain mutation pdf

... (Phe4, Gla7 , Leu8, Gla2 5 and Gla2 9) and EPCR (Leu82,Table 3. Interaction of protein C Gla domain variants with recom-binant wildtype sEPCR. Affinity of protein C Gla domain variants for sEPCR was ... nM). (C) Complete EPCR ⁄ protein C binding cycle. 1, Wildtype sEPCR (800 ng) wasinjected across the flow cell of a CM5 sensor chip coated withRCR-2. sEPCR was injected for 2 min at a flow rate of ... [16,26,27] and is there-fore crucial for its activity.The crystal structures of recombinant sEPCR, and sEPCR in complex with the Gla domain of protein C, have recently been solved [25]. As predicted...

Báo cáo khoa học: The optimization of protein secondary structure determination with infrared and circular dichroism spectra docx

... (1995) Comparison of and limits of accuracy for statistical a nalyses of vibrational and e lectronic circular d ichroismspectra in terms of correlations to and predictions o f protein sec-ondary ... between transmembranehelices and peripheral helices by the deconvolution of circulardichroism spectra of membrane proteins. In Circular Dichroism and the Conformation of Biomolecules (Fasman, G.D. ... theefﬁciency of the secondary structure prediction. Clearcomplementarities between IR and CD spectra allow afurther enhancement of the secondary structure accuracy.Experimental proceduresInput...

Báo cáo khoa học: Methylene analogues of adenosine 5¢-tetraphosphate Their chemical synthesis and recognition by human and plant mononucleoside tetraphosphatases and dinucleoside tetraphosphatases pot

... bis(phosphonodichloromethyl)phosphi-nic acid, pCCl2pCCl2p free acid.Synthesis of adenosine-5’-[b ,c, c,d-bis(dichlorometh-ylene)]tetraphosphate, pCCl2pCCl2ppA. Synthesis of a,b;b ,c- bis(methylene)-ATP, ... b ,c- methyleneATP.Neither of these explanations accounts for the muchreduced activity of pppCH2pA for the human p4AFig. 3. Time course of p4A (A) and pppCH2pA (B) hydrolysis catalysed by yeast exopolyphosphatase. ... exceed15%. For details of assays see Experimental procedures.Compound Ki(lM)ppppA 10.5pppCH2pA 6.7ppCH2ppA 16.2pCH2pppA 21.8ppCH2pCH2pA 20.0pCH2ppCH2pA 34.0pCCl2pCCl2ppA...

Tài liệu Báo cáo khoa học: Allosteric modulation of myristate and Mn(III)heme binding to human serum albumin Optical and NMR spectroscopy characterization pptx

... 4.3 and 8, in the presence of allosteric effectors, and at pH greater than 8, in the absence of ligands,HSA changes conformation to the basic (B) form withloss of a-helix and an increased afﬁnity ... protons,17O nuclei are negligiblyaffected by dipolar coupling with nearby unpairedelectrons, and the paramagnetic broadening of the17Oresonance is diagnostic of the occurrence of a directcoordination ... homologous domains (labelled I, II, and III), each composed of two (A and B) subdomains.The three domains have different binding capacity for a broad variety of ligands such as aminoacids(Trp and Cys),...

Báo cáo khoa học: Identiﬁcation of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf

... Plasmids for the production of recom-binant protein were constructed by a PCR, where forward and reverse primers encoded the desired parts of the C- and N-termini of GIRK1. These sequences were each ... regulated by cytosolic injections of PKA activators and inhibitors,suggesting it to be the basis for the functional effects of cAMP injections on GIRK currents. PKA-inducedphosphorylation of the recombinant ... phosphorylation of recombinant, truncated, GIRK1 by the catalytic subunit of PKA. In the lower panels, the mean values and standard error of mean values of relative speci c radioactivity of the different constructs...

Báo cáo khoa học: Dual modulation of prothrombin activation by the cyclopentapeptide plactin pptx

... stericallyrestricted arrangement of four hydrophobic aminoacids and a basic amino acid is essential for plactinactivity [2]. Therefore, we speculated that coupling of plactin-14 to CNBr-activated ... ueLehPHNHN2NHNHHNHNNHNHOOOOOHN2syLHNHN2NHNHHNHNNHNHOOOOONHSOONSND-syL01.02.03.04.0u-PA activity (fluorescence intensity)aX + TPaXTPlortnoCD nitcalPFig. 2. Identiﬁcation of prothrombin as a plasma cofactor required for plactin activity. (A) Structures of plactin D and ... (B) The activities of plactin D, plactin-14 and plactin-14–DNS to enhance cellular u-PA activity were measured by incubating eachcompound with U937 cells at the concentrations shown. (C) Human...

Báo cáo khoa học: The modulation of metal bio-availability as a therapeutic strategy for the treatment of Alzheimer’s disease pptx

... precursor protein; CHO, Chinese Hamster Ovary; Cp, ceruloplasmin; CQ,clioquinol; CNS, central nervous system; COX, cytochrome c oxidase; CSF, cerebrospinal ﬂuid; Cu ⁄ Zn-SOD, copper ⁄ zinc superoxidedismutase; ... R, Duce JA, Ciccotosto GD, Li QX,Barnham KJ, Curtain CC, Cherny RA, Cappai R,Dyrks T et al. (2005) Copper-dependent inhibition of human cytochrome c oxidase by a dimeric conformer of amyloid-b1)42. ... McIntire TM, MiltonSC, Cotman CW & Glabe CG (2003) Common struc-ture of soluble amyloid oligomers implies commonmechanism of pathogenesis. Science 300, 486–489.48 Atwood CS, Scarpa RC,...

Báo cáo khoa học: "The Modulation of Cooperation and Emotion in Dialogue: The REC Corpus" pdf

... The idea of evaluating utterance choices in rela-tion to task success can be traced back to Ander-son and Boyle (1994) who linked utterance choic-es to the accuracy of the route performed on ... automatic calculated as number of heart beats per minute. Galvanic Skin Conduc-tance (SK) was recorded with Ag AgC1 elec-trodes attached to the palmar surface of the second and third fingers of ... Language and Speech, 34:351-366 Anderson A. H., and Boyle E. A. 1994. Forms of in-troduction in dialogues: Their discourse contexts and communicative consequences. Language and Cognitive Process...

Báo cáo khoa học: Selective inhibition of ADAMTS-1, -4 and -5 by catechin gallate esters ppt

... native codingsequence. External primers 5¢-GAAGATCTGACTACAAGGACGACGATGAC-3¢ (sense) and 5¢-CCTCTAGATTACTAACATTTCTTCAACAAGCATTG-3¢ (antisense)containing a BglII and XbaI restriction site, respectively,were ... pIB/V5-His/TOPOÒ vector using two primers, 5¢-CCGATCTCAATATGAAGTTATGC-3¢ (sense) and 5¢-CCTCTAGATTACTAACATTTCTTCAACAAGCATTG-3¢(antisense) and the TOPO TA cloningÒ methodologyaccording to the manufacturer’s ... 1.7% for ADAMTS-1, -4 and -5, respectively.In summary, the addition of type-speci c covalentinactivators of serine and cysteine proteinases, the lack of cleavage of a quenched ﬂuorescence substrate...

Báo cáo khoa học: "SELECTIVE PLANNING OF INTERFACE EVALUATION" pdf

... and some of the discus=dons of the heat of re(retry, but the critical details are completely out of reach. Rather than carefully controlled evaluations, the public needs competent and honest ... SELECTIVE PLANNING OF INTERFACE EVALUATION~ William C. Mann USC Information Sciences Institute 1 The Scope of Evaluations The basic ides behind evaluation is 8 simple one: An object is ... effects. The effects come from the threat of an evaluation, and they are like the threat of a military inspection. All of the valuable effects are complete before the ins~oection takes piece....

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