Báo cáo khoa học: Microcin J25 induces the opening of the mitochondrial transition pore and cytochrome c release through superoxide generation doc

... MccJ25 on the respira-tory chain forces the opening of the MTP pore, whichis mediated by an increase of the matrix calcium con-centration, with the concomitant release of cyto-chrome c. The ... intramitochond-rial calcium concentration and the opening of MTPtrigger mitochondrial swelling, with the concomitant release of the apoptotic inducer cytochrome c [24]. The sequence of these events ... 5-(-6)-carboxy-2¢,7¢-dichloro-dihydrofluorescein; CsA, cyclosporin A; DNP, 2,4-dinitrophenol; FITC, fluorescein isothiocyanate;MccJ25, microcin J25; MccJ25F*, fluorescent derivative of microcin J25; MTP, mitochondrial...

Tài liệu Báo cáo khoa học: Phylogenetic relationships in class I of the superfamily of bacterial, fungal, and plant peroxidases pptx

... pseudomalleiCandalbCCP Contig19–10046* Cytochrome c peroxidase Candida albicansCapsannAPX Q84UH3 Ascorbate peroxidase Capsicum annuumCaulcreCP O31066 Peroxidase/catalase Caulobacter crescentusChlamspAPX ... formation of the c orresponding CCP compound I [ 37]. Site-directedmutagenesis was performed in the proximal heme cavity of SyncyspCPn [38] and SacchceCCP [34] and focused on the function of the two ... Site-directed mutagenesis in E_coliHPIn [31],MycotubCPn [32], and SyncyspCPn [29], a s well a s inPisusatAPX [33] and SacchceCCP [34], supported the role of the catalytic triad by affecting the...

Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx

... environments in the two molecular species. In the case of the short Tat CPP, the cluster of b asic amino acids is likely to be fully ac cessible tocellular components inducing the translocation event, ... concentration of 1 lgámL)1in the presence of 100 lMchloroquine in the cellculture medium. For FACS analysis, the concentration of the recombinant protein was increased to 5 lgámL)1. The ... alter the uptake of the Tat CPP. Thesebiochemical evidences con®rmed a pathway for the e ntry of the Tat peptide unrelated to the HS proteoglycan receptors.Internalization of the Tat CPP did...

Báo cáo khoa học: O-MACS, a novel member of the medium-chain acyl-CoA synthetase family, speciﬁcally expressed in the olfactory epithelium in a zone-speciﬁc manner doc

... (5¢-GAATTCatgaaggttctcctccactg-3¢) and rO-MACS-XhoI-D (5¢-CTCGAGtgcccgtccccactcctggt-3¢)for o-macs; EcoRI-mMACS1-U (5¢-GAATTCatgcagtggctgaagagttt-3¢) and mMACS1-XbaI-D (5¢-TCTAGAtagctgaccaaactccttg-3¢)forMACS1, and cloned ... o-macs,5¢-atgaaggttctcctccgctg-3¢(forward), 5¢-gcctttcgggacaaggagcc-3¢ (reverse); SA 5¢-aggtgttttcagcgcctagc-3¢ (forward), 5¢-caccattactctgtctcctc-3¢ (re-verse); KS 5¢-ccttctggggcactgagatg-3¢ (forward), 5¢-agaacgcatgcagccgaggg-3¢ ... OCAM5¢-gagaagtggtgtcccctcaa-3¢ (forward), 5¢-cctccatcatcttgcttggt-3¢ (reverse); NCAM 5¢-cttcctgtgtcaagtggcag-3¢ (for-ward), 5¢-gttggcagtggcattcacga-3¢ (reverse); and NeuroD5¢-aagacgcatgaaggccaatg-3¢...

Báo cáo khoa học: "GRAMMATICAL AN ALYSIS BY COMPUTER OF THE LANCASTER OSLO/BERGEN (LOB) CORPUS OF BRITISH ENGLISH TEXTS." potx

... solved if the system is to become completely automatic and capable of correct tagging of standard text. Constituent ;alalysis. The high success rate of word tag selection achieved by the one-step ... weighted according to whether the form occurs at the beginning of the sentence or elsewhere. Knut Hofland estimated a success rate of about 9a.3 per cent without pre-editing (Leech, Garside and ... LOB CORPUS. The LOB Corpus (Johansson, Leech and Goodluck, 1978), like its American ~/gl~sh counterpart, the Brown Corpus LKucera and Francis, 196a; Hauge and ;Iofland, 1978), is a collection...

Báo cáo khoa học: Broad antibiotic resistance proﬁle of the subclass B3 metallo-b-lactamase GOB-1, a di-zinc enzyme potx

... reverse:(5¢-GATCTTGCTGCTTACTCATGCTCACTACGACCATACAGG-3¢)(5¢-GCACCTGTATGGTCGTAGTGAGCATGAGTAAGCAGC-3¢)Production and puriﬁcation of the zincb-lactamaseLB medium (100 mL) containing 50 lgÆmL)1kanamycinwas ... reverse:(5¢-GATCTTGCTGCTTACTGCGGCTCACTACGACCATACAGG-3¢)(5¢-GCACCTGTATGGTCGTAGTGAGCCGCAGTAAGCAGC-3¢)For the Q116N mutant forward and reverse:(5¢-GATCTTGCTGCTTACTAACGCTCACTACGACCATACAGG-3¢)(5¢-GCACCTGTATGGTCGTAGTGAGCGTTAGTAAGCAGC-3¢)For the Q116H mutant forward and reverse:(5¢-GATCTTGCTGCTTACTCATGCTCACTACGACCATACAGG-3¢)(5¢-GCACCTGTATGGTCGTAGTGAGCATGAGTAAGCAGC-3¢)Production ... and Xho1 restriction sites were introduced at eitherend of the blaGOB-1gene by PCR using the oligonucleotideprimers (5¢-GGGGGGGGATCCATGAGAAATTTTGCTACACTGTTTTTCATG-3¢) and (5¢-CCCCCCCTCGAGTTATTTATCTTGGGAATCTTTTTTTATTTTGTC-3¢),...

Báo cáo khoa học: Cellulose crystallinity – a key predictor of the enzymatic hydrolysis rate pptx

... slowdown[14,29,30].One of the most controversial theories concerns the inﬂuence of crystallinity and the change of the degree of crystallinity during enzymatic hydrolysis. It isaccepted that the initial degree of ... about the details of the mecha-nistic action of cellulase and the hydrolysable ⁄ reactive fractions of cellulosechains. X-ray diffraction provides insight into the mode of action of Cel7Afrom ... rate[51]. A recent study using phosphoric acid to increasecellulose accessibility in lignocellulosics suggested the presence of a critical point in the phosphoric acid con-centration below which enzymatic...

Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf

... described [47]. Probes for this assay wereoligonucleotides representing the CT element from the human c- myc promoter: CT (sense): 5¢-CACCCTCCCCACCCTCCCCATAAGCGCCCCTCCCGGGTTCCCAAA-3¢; and Comp-CT ... organization and amino acid sequence sharing the classical arrangement of seven tandem canonicalCys-Cys-His-Cys (CCHC)-type zinc knuckle domains (C- /-X -C- G-X3-H-X4 -C, where / is an aromatic aminoacid ... single-stranded nucleic acid bind-ing capability. Instead, it promotes in vitro the annealing of complementaryoligonucleotides representing the CT element (CCCTCCCC) from the human cellular myelocytomatosis...

Báo cáo khóa học: Nerve growth factor mediates activation of the Smad pathway in PC12 cells doc

... Infection of the target cells was performed 48 h and 72 h after transfec-tion. Because the retroviral sequences contain the gfp gene,infected cells could be selected by FACS sorting.Luciferase ... Smad4,translocation to the nucleus and gene expression. NGF-mediated transcriptional activation of TGF-b responsivereporter constructs requires the presence of functionalTrkA receptors and can be ... proteins, the inhibitory Smad7, mediates its antagon-istic effects by stable interaction with TbRI, thus preventing the transient contact of R-Smads with the receptor and blocking the proceeding cascade...

Báo cáo khoa học: ERS1 encodes a functional homologue of the human lysosomal cystine transporter pptx

... examine the functional consequence of itsdeletion. The second mutant is ctns-CD82, which lacks the last 82 amino acids at the COOH-terminus of cys-tinosin. This C- terminal domain of CTNS encompasses the ... localized in the vacuole, coincident to the pattern observed by the vacuolar marker, CMAC. However, unlike CMAC,which localizes in the lumen of the vacuole, Gtr1–GFPappears to localize to the ... in these cells of the vacuole lumen ﬂuorescent marker, CMAC. WhileMeh1–GFP and CMAC colocalize to the same com-partment, Meh1 is found at the membrane, whileCMAC is within the lumen (Fig. 4C) ....

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