Báo cáo khoa học: Characterization of the glutamyl endopeptidase from Staphylococcus aureus expressed in Escherichia coli pptx

... nonheatedsamples, the mature form of GluV8 could be renaturedeven after exposure to heat in the presence of SDS.Role of N-terminal Val69 in processing of the GluV8 proformFinally, the role of N-terminal ... for the correct folding of GluV8, indicating its role as an intramolecular chap-erone. Furthermore, the four amino acid residues at the C-terminus of the prosegment were sufﬁcient for both of these ... prosequence of thermo-lysin in enzyme inhibition and folding in vitro. Thisgroup further demonstrated that the prosequence of thermolysin acts as an intramolecular chaperone, evenwhen expressed in...

Báo cáo khoa học: Characterization of c-tocopherol methyltransferases from Capsicum annuum L and Arabidopsis thaliana pptx

... analysis of fractions obtained during subsequentsteps of c-TMT purification from Capsicum fruits. From each step, 1 lg of protein was loaded on the gel. Proteins were visualized by silverstaining. The ... 4A).Comparison of the properties of the partially purifiedc-TMT from Capsicumfruits with the recombinantc-TMT from ArabidopsisRecombinant Arabidopsis c-TMT containing a His-tag waspurified from E. coli ... linesconverging to the left of the ordinate axis. Secondary plots of V)1intercepts and of slopes were linear for either of the substrates. These results indicated that the c-TMT methy-lation reaction...

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... with the ﬁrst 88 aminoacids of PPI1 [22], indicating that the PM H+-ATPasebinding site of PPI1 is localized therein. Indeed, fusionproteins containing the ﬁrst 88 amino acids of PPI1,linked ... suggested by the parallel increase of the number of FC-bindingsites [18–20]. As to auxin, a soluble auxin receptor hasbeen reported to bind and activate the PM H+-ATPase,but the site of binding has ... activity of the enzyme whoseC-terminus has been displaced by low pH or byFC-induced binding of 14-3-3s.Results The C-terminus of isoform 1 of the PM H+-ATPase of A. thaliana (AHA1) interacts...

Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

... of the genes. In SNO1, the 199th G from the 5¢ terminus of the open readingframe was deleted to result in a frame-shift and appearance of stop codons in the downstream. As a result, a protein ... or two of the recombinant plasmids. The resulting cells over -expressed the desired protein(s) up to15% of the total cellular proteins. When Sno1p was expressed alone, it was kept in the inclusion ... that the nitrogen of pyridoxine is derived from the amide group of glutamine in yeast [4], while glutamate is the source of the ring nitrogen in E. coli [5]. Recently, two independent groups...

Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc

... binding factors. The introduction of mutation in one of the Ets-bindingsequences greatly decreased its promoter activity, suggestingthat the transcription of the a3 integrin gene in these cells ... includingEts, GATA, and MyoD/E-box binding factors. The introduction of mutation into one of the putative Ets-binding sequences suppressed the promoter activity. In addition, the speciﬁc binding ... assay (EMSA) using the Ets consensus site at )133As the involvement of the Ets consensus binding site at )133 in the promoter activity of the mouse a3 integrin gene wassuggested by the luciferase...

Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt

... suggested a single cut at the C9–C10 double bond of the ring-bearing side of 3-OH-c-carotene in combination with several cleavageoptions in the linear half of the molecule, namely at the C9¢–C10¢,C7¢–C8¢ ... temperature of 340 °C, which was maintained for an addi-tional 5 min. The He carrier gas ﬂow was maintained at1mLÆmin)1using a split ﬂow of 1 : 20. The splitless timewas 3 min, and the injector ... investigate the activity of OsCCD1, the correspond-ing cDNA was cloned and expressed as a glutathioneS-transferase (GST)-fusion protein in Escherichia coli cells. However, the insolubility of the fusion...

Báo cáo khoa học: Characterization of the NADH:ubiquinone oxidoreductase (complex I) in the trypanosomatid Phytomonas serpens (Kinetoplastida) ppt

... arrows).Owing to the horizontal smear, however, the presence of the B14 subunit in the lower band cannot be ascer-tained at this point. A cross-reaction of the antibodyagainst the 39 kDa subunit of ... Duve Institute of Cellular Pathol-ogy) for critical reading of the manuscript. This workwas supported by grants from the Grant Agency of the Ministry of Education of the Slovak Republicand the ... are speci-ﬁc inhibitors of complex I in eukaryotes, we assumethat they bind to the large form of the complex,which contains a homolog of the B14 subunit of mammalian complex I. The residual...

Báo cáo khoa học: Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli pot

... the N ter-minus of ribosomal protein L11 in regulation of the RelA protein of Escherichia coli. J Bacteriol 183, 6532–6537.11 Thompson J, Cundliffe E & Stark M (1979) Binding of thiostrepton ... [7].tRNA-dependence of pppGpp synthesisBinding states of tRNAsIt was important to determine the binding states of tRNAs in ribosomal complexes that stimulatedpppGpp synthesis by SF as tRNAs could either ... retained on the ﬁlter in the presence of SF (Table 2). The effect was speciﬁc to SF, as incu-bation of unacylated tRNA with recombinant ribo-somal protein L10 or ovalbumin did not increase the amount...

Báo cáo khoa học: Characterization of a prokaryotic haemerythrin from the methanotrophic bacterium Methylococcus capsulatus (Bath) ppt

... NorwayHaemerythrin proteins comprise a family of O2-carrry-ing proteins mainly found in a few phyla of marine invertebrates. Members of this family differ from haemoglobin and haemocyanin in that they ... probe made from the PCR fragmentampliﬁed with the primers used in the cloning of MCA0715. The arrowhead indicates the 540 bp transcript, corresponding to the predicted length of the MCA0715 ... haemerythrinFEBS Journal 272 (2005) 2428–2440 ª 2005 FEBS 2433C-terminal end of helix III, pointing towards the inside of the bundle, and Cys128 was located to the randomcoil of the C-terminal end of...

Báo cáo khoa học: Characterization of the bioactive conformation of the C-terminal tripeptide Gly-Leu-Met-NH2 of substance P using [3-prolinoleucine10]SP analogues pdf

... structure. The confor-mations of residues 8–9 are ill deﬁned in the peptides. Thus, the introduction of a prolinoamino acid in the C-terminaltail of SP does not affect the overall structure of the ... spacebeside the absence of c-turn minima.Discussion The nonsubstituted proline analogue [Pro11]SP is only aweak competitor of SP binding sites. Reintroducing the methionine side chain in the pyrrolidine ... cis-andfortrans-prolinoamino acids).Our NMR and modeling studies show that the v1transconformers are stabilized on increasing the bulkiness of the b-substituent. The w angle of the prolinoamino acid...

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