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Solar and Space Physics and Its Role in Space Exploration doc

Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx

Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx

... mutations in the AUH gene. This indi-cated that AUH, in addition to its RNA binding func-tion, must play an important role in leucine catabolism.AUH was overproduced in E. coli and characterizedby ... of leucine catabolism at the protein and DNA levels and developed a novel assay forenzyme analysis in a diagnostic setting. The humanAUH protein was first recognized by its ability to bindA ... For thekinetic characterization of AUH described in the workat hand, AUH was overproduced in Escherichia coli asa maltose binding protein fusion (MBP-AUH). Thecomplete AUH gene (producing MBP-AUHp40...
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Báo cáo khoa học: Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells docx

Báo cáo khoa học: Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells docx

... digest-ible protein substrate, and finally calpastatin.The finding that calpastatin was the most efficientligand at promoting calpain transition, detected as anincrease in mAb that bound to calpain, is ... the intensity of calpain staining increased 7–8-fold, indicating that the protease was now mainlypresent in the high-affinity form. In these conditions, although the highest amount ofcalpain ... only indicate that calpastatin inducesa pronounced Ca2+-independent change in calpainconformation, but also provide strong support for pre-vious observations indicating that calpain and calpast-atin...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... with GST-CD2 (MAP2 v-KIND binding core).Input (cerebellum)GSTv-KIND KIND1v-KIND KIND2Spir-2 KINDPTPN13 KINDInput (MAP2)GSTv-KIND KIND1v-KIND KIND2Spir-2 KINDPTPN13 KINDPD from cerebellar ... stain)EGFPEGFP-KIND2-1EGFP-KIND2-2EGFP-KIND2-3EGFP-KIND2-4EGFP-KIND2-5EGFP-KIND2EGFP-KIND1KIND2-1487KIND2-2521KIND2-3555KIND2-5589KIND2-4488456 620KIND2v-KIND+++––InteractionEGFPEGFP-KIND2-1InputIB: ... structural and func-tional determinants of the protein–protein interactionbetween v-KIND and MAP2 (i.e. the MAP2-bindingcore module in v-KIND and the v-KIND-binding coremodule in MAP2) across...
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Tài liệu Báo cáo khoa học: A novel splice variant of occludin deleted in exon 9 and its role in cell apoptosis and invasion docx

Tài liệu Báo cáo khoa học: A novel splice variant of occludin deleted in exon 9 and its role in cell apoptosis and invasion docx

... findings, our research provides new insights intothe role of exon 9 in the regulation of apoptosis and invasion in liver cell lines.ABFig. 7. Effects of occludin shRNA on apoptosis and invasiveness. ... levelincreased in OccWT-overexpressing cells, and that2-APB inhibited the effects of OccWT. Therefore,the increase in intracellular calcium may explain theinduction of apoptosis and reduction ... Furthermore, in a comparative analysisbetween OccWT and OccDE9, the exon 9 region playeda significant role in promoting apoptosis and inhibitinginvasion by regulating signaling pathways....
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx

Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx

... places, but in particular, stefin B has a numberof histidines in the C-terminus (box in Fig. 1) at sites:92, 75, 66 and 58. As histidine residues are central tocopper binding in many proteins they ... competitor to nonspecific protein copper interactions.A control ITC experiment of titrating stefin B protein withglycine alone was performed and no binding was observed. In our hands, as others [54], ... and Manca Kenig forcloning and isolating the recombinant proteins. Wealso are grateful to Sabina Rabzelj and Sasˇa JenkoKokalj for performing certain SEC experiments and for activity measurements....
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Tài liệu Báo cáo khoa học: Inorganic pyrophosphatase in the roundworm Ascaris and its role in the development and molting process of the larval stage parasites doc

Tài liệu Báo cáo khoa học: Inorganic pyrophosphatase in the roundworm Ascaris and its role in the development and molting process of the larval stage parasites doc

... Pireleased min)1Æmg)1of protein.Enzyme kinetic studyThe Km(Michaelis constant) and Vmax(maximum velocity)values were determined by incubating the diluted recombin-ant proteins in the standard ... at 26 000 g for 30 minat 4 °C. The supernatants containing recombinant proteinsof AsPPase were purified using ProBondTMresin (Invitro-gen) under nondenaturing conditions and subsequentlyeluted ... deparaffinized and dehydrated using a graded series of alcohol and thenrehydrated in NaCl/Pi. The slides were blocked for 30 min in 1% H2O2 in NaCl/Picontaining 10% ethanol to inacti-vate...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

... a proteincontaining a single ankyrin repeat that interacts withHIV-1 viral protein R (Vpr) and we designated thisprotein as Vpr-binding ankyrin repeat protein(VBARP). This interaction was ... aprotinin, and protease inhibitors leupep-tin, chymostatin, and pepstatin (1 lgÆmL)1; Sigma) as des-cribed [14] for 15 min in ice. Cell lysates were centrifugedat 22 000 g for 10 min at ... play an important role in protein–protein interactions. ANKHD1 proteins are known to possessmultiple ankyrin repeat domains and a single KH domain with no knownfunction. Using yeast two-hybrid...
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Tài liệu Corporate Ownership structure and the Informativeness of Accounting Earnings in East Asia doc

Tài liệu Corporate Ownership structure and the Informativeness of Accounting Earnings in East Asia doc

... (Jacobson and Aaker, 1993; Ali and Hwang, 2000). In addition, instead of using NI in our regression model, we have also used ∆NI, change in earnings (current earnings minus lagged earnings all ... complex earnings-generating process, may have weaker earnings-return relations than firms operating in a single industry.15 Finally, we include firm size as a control for other missing factors ... examining how earnings informativeness is affected by the controlling owner’s entrenchment. Using the degree of divergence between voting and cash flow rights as a proxy for controlling owner...
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Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt

Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt

... (4NPGlc), incubating for 30 min at 37 °C, and measuring the rel ease of 4NP.Protein assays and protein sequencingTotal protein in crude and semipuri®ed samples wasestimated using the Pierce Protein ... ncreasingD t o 2 0% (10 min), 50% ( 15 min), 100% (5 min), held at100% (5 min); cyanodin glycosides; 5% B/95% A initial(5 min), increasing B to 20% (10 m in) , 90% (10 min), heldat 90% (5 m in) ; ... (rutin)Quercetin-4¢-GlAKaempferol-3-GlcIsorhamnetin-3-GlcFlavanones Naringenin-7-GlcRha (naringin)Hesperetin-7-GlcRha (hesperidin)Dihydrochalcones Phloretin-7-Glc (phlorizin)Secoiridoids OleuropeinAnthocyanidins Cyanidin-3-Glc (kuromanin)Cyanidin-3,5-diGlcFig....
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The Sun to the Earth -- and Beyond: A Decadal Research Strategy in Solar and Space Physics potx

The Sun to the Earth -- and Beyond: A Decadal Research Strategy in Solar and Space Physics potx

... Earth and BeyondA Decadal Research Strategy in Solar and Space Physics Solar and Space Physics Survey CommitteeCommittee on Solar and Space Physics Space Studies BoardDivision on Engineering and ... future directions of U.S. ground- and space- based programs in solar and space physics research. The NRC’s Space Studies Board and its Committee on Solar and Space Physics orga-nized the study, ... for Solar and Space Physics 53The Sun’s Dynamic Interior and Corona, 54The Heliosphere and Its Components, 57 Space Environments of Earth and Other Solar System Bodies, 58The Role of Theory and...
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