... (4NPGlc), incubating for 30 min at 37 °C, and measuring the rel ease of 4NP.Protein assays and protein sequencingTotal protein in crude and semipuri®ed samples wasestimated using the Pierce Protein ... ncreasingD t o 2 0% (10 min), 50% ( 15 min), 100% (5 min), held at100% (5 min); cyanodin glycosides; 5% B/95% A initial(5 min), increasing B to 20% (10 m in) , 90% (10 min), heldat 90% (5 m in) ; ... (rutin)Quercetin-4¢-GlAKaempferol-3-GlcIsorhamnetin-3-GlcFlavanones Naringenin-7-GlcRha (naringin)Hesperetin-7-GlcRha (hesperidin)Dihydrochalcones Phloretin-7-Glc (phlorizin)Secoiridoids OleuropeinAnthocyanidins Cyanidin-3-Glc (kuromanin)Cyanidin-3,5-diGlcFig....