Báo cáo khoa học: Assessment of porcine and human 16-ene-synthase, a third activity of P450c17, in the formation of an androstenol precursor doc

... Assessment of porcine and human 16-ene-synthase, a third activity of P450c17, in the formation of an androstenol precursor Role of recombinant cytochromeb5 and P450 reductasePenny ... showedelution peaks at 4.70, 2.30 and 2.50 min, respectively(Fig. 2). In both porcine and human assays using preg as a substrate, an additional peak of elution appeared at15 min (panels C and D, respectively). ... DHEA – and analyzed androstadienol formation from each substrate. As observed in Fig. 3, the biosynthesis of androstadienol in humans (A) and pigs (B)does not require prior formation of 1 7a- OH-preg...

Tài liệu Báo cáo khoa học: Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart ppt

... kDa, 622 amino acids), the iron–sulfur protein(Ip, 29 kDa, 252 amino acids), and two membrane-anchor proteins (CybL, 15 kDa, 140 amino acids, and CybS, 11 kDa, 103 amino acids) with a total of ... Lane 1, nucleotide acid markerDL2000 (TaKaRa); Lane 2, CybS subunit. The FP band is not very clear in the agarose gel, and the longest band was used to runanother PCR reaction for ampliﬁcation.X. ... as an effective amphimictic small mole-cule to modulate the interaction between membraneprotein and detergent.Fig. 1. SDS-PAGE analysis of the crystallization preparation and the crystal of...

Tài liệu Báo cáo khoa học: Hypothalamic malonyl-CoA and CPT1c in the treatment of obesity pptx

... increase in malonyl-CoA promotes a decrease in neuropeptide Y and agouti related peptide in hypo-thalamic malonyl-CoA while promoting an increase in proopiomelanocortin and cocaine and amphetamineregulated ... accompanied by a suppression in energyexpenditure. These data suggest that CPT1c canintegrate carbohydrate and lipid nutrient sensing in the brain and is an example of an enzyme that can sense and ... in various organ systems. Even in organismslacking a brain, such as Caenorhabditis elegans, the nervous system plays a key role in maintaining energybalance [1–4]. In more advanced, mammalian...

Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

... in A. thaliana PS. Val111 and Gly113, on the other hand,are both affected by both pantoate and ATP. Theseresidues have their positional equivalents in Val137 and Arg139 in A. thaliana PS, as ... toD-pantoate and ATP are show-n in green. The arrows indicate the sign of change in chemical shift of the panto-ate-bound (P; in blue) and pantoate +ATP-bound (PA; in green) protein ascompared ... is a pantoate bound to the canonicalpantoate-binding site, and Pt* is a second molecule of pantoatebound to the ATP-binding site. ATP at high concentrations candisplace Pt*, and the reaction...

Tài liệu Báo cáo khoa học: Activation loop 3 and the 170 loop interact in the active conformation of coagulation factor VIIa pdf

... afﬁnity, and incorporation of an active site inhibitor into FVIIa and G37 2A- FVIIa increased the afﬁnity for sTF in an indistin-guishable manner. The differences and similaritiesbetween G37 2A- FVIIa ... region and the S1pocket, whereas the intramolecular connections, forinstance between the TF-binding region and the activesite and between the TF-binding region and the activa-tion domain, appear ... enzymatic activity and S1 pocket maturation but at the same time an unaltered conformational distribution of the N-ter-minal tail and a normal response to TF.DiscussionFVIIa contains the canonical activation...

Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc

... bifurcation and the appearance of an unstable limit cycle. The unstable and stable limit cycles collide and annihilate in a global bifurcation near the oscillatory borders.Saturability and consequent ... the SH2 and kinase domains at the back of the small lobe, preventing the formation of a produc-tive catalytic cleft [29]. Thus, these interactions clamp the kinase domain in an inactive conformation ... 3kinase/Akt and MAPK signaling to PDGF-inducedDNA synthesis in mesangial cells. Cell Signal 18, 1854–1864.58 Goryanin I, Hodgman TC & Selkov E (1999) Mathe-matical simulation and analysis...

Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf

... polyclonal anti-bodies raised against each of the WhiB proteins. The absorbanceobtained in the positive control (where the antibody used wasraised against that particular antigen) was set to ... The other impor-tant point is that although the WhiB proteins werepredicted to attain a a-helical structure [16], WhiB5 and WhiB6 appeared to have signiﬁcant amounts of b strand. In WhiB5 and ... conditions indicate importance of each memberseparately in mycobacterial physiology. The availableinformation on WhiB proteins demands careful investi-gation of the biochemical and biophysical properties...

Tài liệu Báo cáo khoa học: Substrate speciﬁcity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt

... cultivated around the world,including the oilseeds rapeseed and canola (Bras-sica napus and Brassica rapa) and vegetables such asecabbage (Brassica oleraceae var. capitata), cauliflower(B. oleraceae ... out using integration of peak areas of 3-indolylmethanamine and a calibration curve of indolyl-3-methanamine (3).Kinetic analysis and protein measurementsKinetic parameters of the purified enzyme ... L. maculans and A. brassicicola catalyse the detoxification of brassinin by hydrolysis of its dithiocarbamate groupto indolyl-3-methanamine. The purification and characterization of brassi-nin...

Tài liệu Báo cáo khoa học: Identiﬁcation, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

... zinc-containing enzymes catalyzing the reversible hydration of CO2to bicarbonate. Ubiqui-tous in a wide range of eukaryotic organisms, they arealso widespread in the Archaea and Bacteria domains[11]. ... sections of the branchial plume and of the trophosome asshown in Fig. 2A. The branchial plume is composed of a central obturaculum, mainly made of extracellularmatrix, supporting many branchial ﬁlaments ... overexpression approach of RpCAbr and RpCAtr, we cannot know the functionaleffect of changes of some key amino-acids.Origin and number of nonvertebrate CAsAlthough the bootstrap values of the deep branches...

Tài liệu Báo cáo khoa học: ATP-dependent modulation and autophosphorylation of rapeseed 2-Cys peroxiredoxin docx

... using first the 5¢-pri-mer 5¢-GGGATTCAAGCCTGGGGAGAAATC-3¢ and the 3¢-flanking T7 terminator and subsequently the 3¢-mega-primer and the 5¢-flanking for the T7 promoter. Afteramplification, all ... suchas reductants and ROS. An imbalance between thesedual functions is probably associated with many human pathologies, such as thyroid tumors, breast and lung cancer, Alzheimer’s disease and ... addition of EGTA to a final concentration of 5 mM, the protein solution was further incubated for 5 min and the peroxidase activity was assayed as in (A) . (D) Effect of ATP and Mg2+on the chap-erone...

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