Báo cáo khoa học: Homoadenosylcobalamins as probes for exploring the active sites of coenzyme B12-dependent diol dehydratase and ethanolamine ammonia-lyase docx
Báo cáo khoa học: Homoadenosylcobalamins as probes for exploring the active sites of coenzyme B12-dependent diol dehydratase and ethanolamine ammonia-lyase docx
... usethem as probes for exploring the active sites of enzymes, the coenzymicproperties of homoadenosylcobalamins for diol dehydratase and ethanol-amine ammonia-lyase were investigated. The kcat and ... [24] and AdoEtCbl[29] were reported to be totally inactive as coenzyme for ribonucleotide reductase, and the latter inactive for diol dehydratase [27]. The X-ray structure of the diol dehydratase AdePeCbl ... 4787–4796 ª 2005 FEBS Homoadenosylcobalamins as probes for exploring the active sites of coenzyme B12-dependent diol dehydratase and ethanolamine ammonia-lyase Masaki Fukuoka1, Yuka Nakanishi1,...
- 10
- 444
- 0
Báo cáo khoa học: Peroxiredoxins as cellular guardians in Sulfolobus solfataricus – characterization of Bcp1, Bcp3 and Bcp4 pot
... Fe(NH4)2(SO4)2 and 0.1 mL of 2.5 m KSCN,using H2O2 as a standard. The amount of the ferrithiocya-nate complex present was determined by measurement of A490. The percentage of peroxides removed was ... from the S. solfa-taricus P2 genome: we report on the cloning of bcp1,bcp3 and bcp4 and the characterization of the recombi-nant products and, next, we investigate the possiblerole of the ... structure.Peroxidase activity The peroxidase activity of recombinant Bcps was testedin vitro via a metal ion catalyzed oxidation (MCO)assay. The assay was based on the ability to protect plas-mids...
- 11
- 439
- 0
Báo cáo khoa học: C fi G base mutations in the CArG box of c-fos serum response element alter its bending flexibility Consequences for core-SRF recognition potx
... poly-morphism of the (A ⁄ T) domain and the dynamics of the bent form one determinant for specific complexformation. They appear to be as important as the con-servation of the DNA base sequence. Therefore, ... tem-peratures. The increase in intensity of the 926 (924),1444 and 1462 cm)1vibrational bands of deoxyribose and of the 790 and 1056 cm)1bands of backbonereflects the disappearance of this linear ... modulates the inducible con-formational properties of SRE [23,24]. The origin of the adequate conformational deformation of the CArGboxes lies in the polymorphism of the (A ⁄ T) domain.Yet within the...
- 16
- 538
- 0
Tài liệu Báo cáo khoa học: "Enhanced word decomposition by calibrating the decision threshold of probabilistic models and using a model ensemble" pdf
... Hafer and Weiss, 1974). Another way of classifying ap-proaches is based on the learning aspect during the construction of the morphological model. If the data for training the model has the same ... precision=f -measure·recall2recall−f -measure and are hy-perboles. For increasing f-measure values the iso-metrics are moving further to the top-right corner of the plot. For a threshold of h = 0.50 ... net increase of 0.0819 of correct boundaries which led to the in-creased recall. Since the deduction of precisionis less than the increase of recall, a better over-allperformance of PROMODES-H...
- 9
- 557
- 0
Báo cáo khoa học: Nanoparticles can induce changes in the intracellular metabolism of lipids without compromising cellular viability docx
... are known sites of nanoparticle accumulation, could provide useful measures of nanoparticlesafety. Such assays would expand the current platform of tests for the determination of the biocompatibility ... lgÆmL)1)in the presence of the fatty acid synthase (FAS) inhibi-tor cerulenin (5 lgÆmL)1). FAS is responsible for the synthesis of FFAs, which can then be esterified toform components of cell membranes ... release of FFAs, which thenwould be available for esterification and the formation of triacylglycerols (the main components of LDs). Weconsidered such a possibility; however, the inhibitionof...
- 14
- 540
- 0
Báo cáo khoa học: A ribonuclease zymogen activated by the NS3 protease of the hepatitis C virus potx
... conformational stability of the 1Czymogen increases on activation, perhaps as the result of the release of strain.Affinity for ribonuclease inhibitor and cytotoxicityRI recognizes members of the ... provides a measure of the therapeutic index of a ribonuclease zymogen. For the 1C zymogen, the (kcat⁄ Km)activated⁄ (kcat⁄ Km)unactivatedratio is 105 for the 1C zymogen and 13 for the 2Czymogen. ... by the NS3 protease, the ribonucleolytic activ-ity of the RNase A zymogen increased 105-fold. Both before and after acti-vation, the RNase A zymogen displayed high conformational stability and evasion...
- 9
- 391
- 0
Báo cáo khoa học: DNA polymerase e associates with the elongating form of RNA polymerase II and nascent transcripts pot
... replicases in the eukary-otic cell [22]. Therefore, we tested the effect of the replicative state of the cell on the interaction betweenPol e and RNA pol II. A possible confinement of the interaction ... between the hypophosphorylated initiation isoform IIA and the hyperphosphorylated elongating isoform IIO thatexhibits reduced electrophoretic mobility comparedwith the IIA isoform. Whereas the ... PDG-1E8, and a combination of G1A, H3B and E24C wereused for detection of Pols a, d and e, respectively.Indirect immunofluorescenceT98G cells were grown on glass slides to logarithmic phase and washed...
- 15
- 584
- 0
Báo cáo khoa học: Akt-dependent phosphorylation negatively regulates the transcriptional activity of dHAND by inhibiting the DNA binding activity pdf
... indicate the positions of dHAND mutants. (B) Coimmunoprecipitation assay of dHAND and Akt. AU5-dHAND was immunoprecipitated from the cell lysate of 293T c ells e xpressing AU5-dHAND and HA-Akt. Thesesamples ... of dHAND-WT/E47. The activity of dHAND-Ala-X/E47was p artially reduced by coexpression of Akt. But thisinhibitory effect of Akt was not detected in the case of dHAND-Ala/E47 (Fig. 6A).Luciferase assay was ... phosphorylation of these sites might affect the DNAbinding and/ or dimerization activities of dHAND. We firstanalyzed the effect of phosphorylation on the transcriptionalactivity of dHAND using reporter assay...
- 10
- 483
- 0
Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
... significantlyincreases the cost of the purification, and the require-ment for HPLC increases the length and complexity of the purification procedure. Moreover, as with the studies by Lee et al. [21] and Subramanian ... Subramanian and Shree[42], there was no rigorous assessment of the purity of the product or the correctness of the sequence. In con-trast, the purification protocol that we have developedis quick and ... USA), and purified using reverse-phase HPLC. For both synthetic and recombinant Ab peptides, the correct mass was confirmed by MALDI-TOF MS and LC-MS.PCR and cloning procedureSynthetic genes for...
- 16
- 691
- 0
Tài liệu Báo cáo khoa học: "Translation Model Adaptation for Statistical Machine Translation with Monolingual Topic Information" doc
... phrase tables with these two meth-ods, and then respectively use them in place of the out -of- domain phrase table to test the system perfor-mance. For the purpose of studying the generality of our ... probabilities, has a greateffect on the performance of SMT system. Phraseprobability measures the co-occurrence frequency of a phrase pair, and lexical probability is used to vali-date the quality of the ... emphasis on solving the translationambiguity by the exploitation of the context in-formation at the sentence level, we adopt the topical context information in our method for the following reasons:...
- 10
- 533
- 0
Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Báo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíChuong 2 nhận dạng rui roQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)chuong 1 tong quan quan tri rui roNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)