Báo cáo khoa học: High negative charge-to-size ratio in polyphosphates and heparin regulates factor VII-activating protease pdf

... low-molecular-weight heparin and N-acetyl heparin promotean increase in FSAP activity rather than inhibitingit. Thus, polyanion binding to SERPINs, over and above their binding to FSAP, plays a decisive role in ... potential for binding to and activating FSAP.The heparin homologues N-acetyl heparin, de-N-sul-fated heparin and N-acetyl-de-O-sulfated heparin, which have the same size but reduced negative charge,neither ... Polyanions and FSAPFEBS Journal 276 (2009) 4828–4839 ª 2009 The Authors Journal compilation ª 2009 FEBS 4839 High negative charge-to-size ratio in polyphosphates and heparin regulates factor VII-activating...

Tài liệu Báo cáo khoa học: Cytokinin oxidase/dehydrogenase genes in barley and wheat docx

... chmu¨lling, T. (2003) Cytokinin-induced upregulation ofcytokinin oxidase activity in tob acco includes changes in enzymeglycosylation and secretion. Physiol. Plant. 11 7, 11–21.41. Tingay, ... seedlings.The measured v alues are summarized in Table 2. The totalcytokinin content in the grains was approximately threefoldlower than in young seedlings. The increase was mainlyobserved in ... Werner,T.,Motyka,V.,Laucou,V.,Smets,R.,vanOnckelen,H.&Schmu¨lling, T. ( 2003) Cytokinin-deﬁcient transge nic Arabi-dopsis plants show multiple developmental alterations indicatingopposite fun ctions of cytokinins in the regulation of shoot and root...

Tài liệu Báo cáo khoa học: Evidence for proteasome dysfunction in cytotoxicity mediated by anti-Ras intracellular antibodies pdf

... obtained to date indicate thatinhibition of Ras activity suppresses cell proliferation and induces regression in a broad range of tumors.Intracellular antibodies, in particular single-chain ... c), anti-(Ras 1) (d, e and f) and anti-Ras 6 scFv (g, h and i) fragments. Cells were ﬁrst costained in vivo withannexin V (b, e and h) and Hoechst 33342 dye (c, f and i) and, successively, viewed ... lMlactacystin (compare the scPs band in lanes 3 and 6 ofFig. 3C), and anti-(Ras 5) induced stronger inhibition ofproteasome activity than that obtained with 1 lMlactacys-tin (compare lanes 2 and...

Tài liệu Báo cáo khoa học: "An Alignment Algorithm using Belief Propagation and a Structure-Based Distortion Model" pdf

... co-occurrences. A greedy Viterbitraining is then applied to improve this initialguess. In contrast, our BP/EM training do not needto compute correlation scores and start the trainingwith uniform parameters.We ... (Chauch´e,1984) and for Japanese, the KNP parser (Kuro-hashi and Nagao, 1994).The line SDM:Parsing (SDM standing for“Structure-based Distortion Monolink”) shows theresults obtained by using P-sets ... 2 AER points) by using the sum-product BP,choosing links with high beliefs, and cutting-offlinks with very small beliefs (the cut-off was cho-sen roughly by manually looking at a few alignedsentences...

Tài liệu Báo cáo khoa học: High-afﬁnity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

... (1986) Analysis of serum insulin-like growth factor binding proteins using western blotting: use of themethod for titration of the binding proteins and competi-tive binding studies. Anal Biochem ... demon-stration of rapid insulin-like growth factor II Receptorinternalization and recycling in rat adipocytes. Insulinstimulates 125I-insulin-like growth factor II degradationby modulating the ... decrease in PMP-BSA binding. It thusappears that tethering of the C-terminal end of theectodomain is important for both IGF-II and PMP-BSA binding. In summary, the major ﬁndings obtained in thepresent...

Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx

... procedures. Finalprotein concentration was in all cases 45 lM and final concentration of Cu2+46 lM, leading to 1 : 1 of protein to Cu2+ ratio. Results for 1 : 3 pro-tein to Cu2+ ratio have ... places, but in particular, stefin B has a numberof histidines in the C-terminus (box in Fig. 1) at sites:92, 75, 66 and 58. As histidine residues are central tocopper binding in many proteins they ... competitor to nonspecific protein copper interactions.A control ITC experiment of titrating stefin B protein withglycine alone was performed and no binding was observed. In our hands, as others [54],...

Tài liệu Báo cáo khoa học: Single phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-afﬁnity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein ppt

... Tyr304 in the cytoplasmic tail of ephrin B2confers high- affinity and bifunctional binding to both the SH2 domainof Grb4 and the PDZ domain of the PDZ-RGS3 proteinZhengding Su, Ping Xu and Feng ... CPHYEKVSGDYGHPVYIVQEMP(301–322)for ephrin B2, which was shown to be responsible forGrb4 binding and is identical in both ephrin B2 and ephrinB1 [11]. Strikingly, the Grb4 SH2 domain shows strongbinding to tyrosine-phosphorylated ... site in ephrin B2 thatcreates high- affinity binding to the Grb4 SH2 domain usingsynthetic ephrinB2(301–333) fragments containing single ordouble phosphorylations at five candidate tyrosine residues,Tyr304,...

Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx

... serine proton is the catalytichistidine. Most models use the histidine to shuttle protons:ﬁrst, between the serine and the leaving group; and thenbetween the attacking water and the serine. In ... on pH involving a single pKa[29]:pH ¼ pKaÀ logk À kAkHÀ kThe term k stands for the observed rate, kAstands for the limiting rate at high pH, and kHstands for the limiting rate ... activity will remain. Our results in Fig. 2 show that for A328F 71% remains, for A328I32% remains, for wild-type 40% remains, and for A328G42% remains. In all four cases, the remaining activity...

Báo cáo khoa học: High resolution structure and catalysis of O-acetylserine sulfhydrylase isozyme B from Escherichia coli pot

... Germany In bacteria, archaea and plants, the biosynthesis ofl-cysteine involves l-serine and inorganic sulfur com-pounds [1–5]. In higher animals, however, l-cysteineis derived from l-methionine ... lies in the usualrange and does not change for T68S and R210A. In contrast, mutants Q140A and T68A showed muchhigher temperature dependence factors, correspondingto an appreciable increase in ... Structural insights into catalysis and inhibition ofO-acetylserine sulfhydrylase from Mycobacterium tuber-culosis: crystal structures of the enzyme-a-aminoacrylateintermediate and an enzyme–inhibitor...

Báo cáo y học: " Carotid Intima-media thickness in childhood and adolescent obesity relations to abdominal obesity, high triglyceride level and insulin resistance"

... assessment-insulin resistance were inde-pendent determinants of mean IMT level. Conclusion: Obesity especially abdominal obesity, high TG and insulin resistance may be the main risk predictors of increased ... lg (HOMA-IR) and TG in both bivariate correlation and multiple stepwise linear re-gression analysis, suggesting a link between IMT, insulin resistance and dyslipidemia. Insulin resistance ... adiposity-associated inflammatory factors such as C-reactive protein (CRP), interleukin (IL)-6 and tumor necrosis factor (TNF)-α may play a role in promoting adverse vascular outcomes. The intima media...

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