Báo cáo khoa học: Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme–albumin doc
Báo cáo khoa học: Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme–albumin doc
... Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme–albumin Alessandra di Masi1, Francesca Gullotta2,3, Alessandro ... indicates that the redox and the (un)ligated state of the heme Fe atom allosterically affects ibuprofen binding to (t) HSA–heme.lValues of K2 and K3for ibuprofen binding to tHSA–heme-Fe ... for the first time,that ibuprofen allosterically modulates the spectro-scopic and reactivity properties of tHSA–heme and HSA–heme by binding to the low-affinity secondary sites FA2 and FA6 rather...
- 9
- 489
- 0
Tài liệu Báo cáo khoa học: Calcium-binding to lens bB2- and bA3-crystallins suggests that all b-crystallins are calcium-binding proteins pptx
... cal-cium displaces the dye bound to calcium -binding sites of the protein. Other proteins of this superfamily,namely c-crystallin [20] and AIM1-g1 [15] also induce the J band of the dye indicating ... 492 and 547 nm (Fig. 3). The enhancedluminescence of terbium in the presence of these crys-tallins indicates that Tyr and Trp residues are in the vicinity of the calcium -binding site. The sequence ... filtration and dynamic lightscattering of the apo and holo forms of bA3- and bB2-crystallins. There was no significant change in the stability and hydrodynamic radius of the both forms of proteins...
- 13
- 449
- 0
Tài liệu Báo cáo khoa học: Covalent binding to glutathione of the DNA-alkylating antitumor agent, S23906-1 doc
... the bionucleophiles DNA and GSH. The covalent binding of the drug to GSH reduced the formation of DNA adducts and slightly decreased the cytotoxic potential of the molecule. There is now little room ... residues at the N2 position in double-stranded DNA [11]. The covalent binding to DNA isapparently responsible for the cytotoxic action [12] and the capacity of the drug to trigger apoptosis in ... the cellular level, the presence of GSH slightly reduces the cytotoxic potential of S23906-1 towards KB-3-1 epidermoidcarcinoma cells. The GSH-induced threefold reduction of the cytotoxicity of S23906-1...
- 12
- 701
- 0
Báo cáo khoa học: Nucleotide binding to human UMP-CMP kinase using fluorescent derivatives ) a screening based on affinity for the UMP-CMP binding site potx
... screening of newcompounds.Results and DiscussionCompetitive fluorescence experiments to determine the binding of natural substrates to the donor and the acceptor sites of human UCK The ATP -binding ... 6. Model of the acceptor -binding site in the closed form of human UCK. (A) Superposition of the acceptor site with bound CMP (blue) and cidofovir (red). (B) Superposition of the acceptor site ... the phosphategroup of dCMP. This prevents the transfer of the c-phosphate from ATP. The flexibility of the acyclicpart of the tested compounds may prevent these inter-actions. The size of the...
- 11
- 468
- 0
Báo cáo khóa học: Emerin binding to Btf, a death-promoting transcriptional repressor, is disrupted by a missense mutation that causes Emery–Dreifuss muscular dystrophy pdf
... digested withEcoRI and SalI, and inserted into the p-GBK vector(Clontech Laboratories, Inc., Palo Alto, CA, USA) using the SalIandBamHI sites in the vector. The DNA sequences of all fusion plasmids ... as binding to other proteins at the inner nuclear membrane.Two of emerin’s known binding partners are lamin A and barrier -to- autointegration factor (BAF) [4,5]. The 40-residue ÔLEM-domainÕ of ... required for binding to BtfA functional map of emerin was defined previously, withrespect to binding partners BAF and lamin A [5], and twoother binding partners [30,31]. To map the binding site...
- 11
- 377
- 0
Báo cáo khoa học: "From Single to Multi-document Summarization: A Prototype System and its Evaluation" pptx
... bigrams, and trigrams1, using the on- topic document collection as the relevant set and the off-topic document collection as the irrelevant set. Figure 1 shows the top 5 concepts with their ... score in the upper range of 2 to 3 and all others had scores lower than 2.5. Some of the systems (including B2) fell into the range of 1 to 2 meaning some or hardly any cohesion. The lead ... 20.69% 15.57% 3.04% the best, followed by N, and then T; if we choose averaged weighted retention (Rw all), T is the best, followed by N, and then Y. The reversal of T and Y due to different metrics...
- 8
- 288
- 0
Báo cáo khoa học: Well-defined secondary structures Information-storing molecular duplexes and helical foldamers based on unnatural peptide backbones pot
... oligoamidesconsisting of meta-substituted benzene rings linked byglycine residues. The amide O and H atoms act as H-bonddonor and acceptor sites. Various arrangements of the amide O and H atoms lead to Ôhydrogen ... 0.10) and the 1 : 1 mixture (Rf¼ 0.96) clearlyindicated the formation and high stability of the duplex.Further NMR studies showed significant downfield shifts of the amide protons of the 1 ... 1 mixture of 1 and 2in comparison to the separate solutions of the single strands.Attempts to determine the dimerization constant by NMRfailed as no upfield shift of the amide protons was detectedupon...
- 10
- 390
- 0
Báo cáo khoa học: Tetracysteine-tagged prion protein allows discrimination between the native and converted forms pptx
... arsenic atoms in FlAsHenables it to bind with high affinity and specificity to the TC motif introduced into a variety of different proteins[15]. The binding of FlAsH reagent and also the quan-tum ... quantify the amount of nonconverted TC-tagged PrP by measuring the fluo-rescence after the addition of FlAsH. The total initialamount of TC-tagged PrP in the sample required for the normalization and ... method to determine the fraction of converted PrP, based on the ability of the fluorescein arseni-cal helix binder reagent to differentiate between the monomeric and fibril-ized form of TC-tagged...
- 13
- 447
- 0
Báo cáo khoa học: Retrocyclin RC-101 overcomes cationic mutations on the heptad repeat 2 region of HIV-1 gp41 ppt
... RC-101 doc- kings to dimer and comparative monomer macromoleculeswere prepared using autodocktools (ADT) and accompa-nying scripts, and then run with autodock 3.0 and auto-grid 3.0 [28,29]. The ... near the a-helix to which they were docked. The RC-101 mole-cules docked to HR1 are much more dispersed than the RC-101 molecules docked to HR2. The color of each residue of HR1 and HR2 in (A)correlates ... preferentially docks to the HR1 -binding domain of HR2. (A) The top five docked RC-101 molecules for a representative dock-ing, as measured by the final docked energy, are shown as gray loops near the a-helix...
- 11
- 351
- 0
Báo cáo khoa học: What’s in a covalent bond? On the role and formation of covalently bound flavin cofactors doc
... bothcovalent bonds of FAD to the polypeptide chain of ChitO are required for correct positioning of the flavin to facilitate efficient catalysis. The double anchoring of FAD allows the protein to evolve ... stability, ensure cofactor binding, and orinduce a relatively high redox potential of the cofactor.Although the experimental data may show that a cova-lent bond is there to increase the redox potential, ... of MAO B. The isoalloxa-zine ring of FAD is in yellow. The axis of the pink helix pointsdirectly at the C2-O of the isoalloxazine. Cys397, covalently bound to the 8a-carbon of the isoalloxazine...
- 23
- 564
- 0
Từ khóa: Báo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018chuyên đề điện xoay chiều theo dạngNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015Đổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namMÔN TRUYỀN THÔNG MARKETING TÍCH HỢP