Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx
Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx
... isolate the first reaction step (substrate cleavage) by preventing the second reaction step (exon ligation). In addition, the product of the first reaction step is actually the intermediate in the ... 2008 The Authors Journal compilation ª 2008 FEBS Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction P. Patrick Dotson II*, Joy Sinha* and Stephen ... substrate-cleavage reaction is that the second reaction step of TES (exon–ligation) proceeds immediately after the first step [20]. To prevent the second reaction step while allowing the first, we previously...
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Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding specificity and target candidates doc
... Scheme of the three-hybrid strategy used in the screen. (B) Number of colonies identified in each step of the screen. (C) Distribution of the 63 distinct sequences in relation of their position in the ... similar to that of other well-characterized members of PUF family. These proteinsmust bind to transcripts in the plant and regulate theirtranslation and ⁄ or turnover.A large number of APUM target ... in the interactions between APUM-2 and the FAS-2 transcripts with substitutions in the nucleotides down-stream of the APBE. (D) Quantitative analysis of LacZ activation in the interactions of...
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Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... superoxide in the course of the reaction. The principle of the assay is that the presence of aSOD inhibits the reduction of cytochrome c. This was vali-dated by adding a total of 2.5 U of commercial ... (1971) The kineticsand mechanisms of the reactions of iron(III) with b-dik-etones. The formation of monoacetylacetoatoiron(III)and the effect of copper (II) on the formation of monothenoyltrifluoroacetonatoiron ... fittedbackground and the spectrum was integrated. The apparentedge energy of the sample was determined as the maximum of the first derivative of the XANES spectrum. The extracted Fe K-edge (25–730 eV) EXAFS...
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Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf
... the titrant. The active siteconcentration of wild-type BuChE was 0.16 ± 0.01 lm,and that of the D70G mutant was 0.196 ± 0.02 lm.Effects of ligands on steady-state kinetics The effects of the ... 1) and therefore b ⁄ a >3. The difference in the extent of activation can beexplained by differences in the rate-limiting steps.Because the rate-limiting step for the hydrolysis of o-NAC ... (bkcatwith b > 1). The degree of activation in the presence of tyraminewas higher for the hydrolysis of o-NAC than for the hydrolysis of o-NPA. This was determined from the nonactivated and...
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Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx
... con-centration of 4 mm, HpSDH displayed no activity,either in the presence of NADP or NAD. In compar-ison with the kinetic parameters of SDH enzymes from the other bacteria shown in Table 1 [23,26,27], the ... Kmvalues of HpSDH are similar to those of A. fulgidusSDH (AfSDH), but the kcatvalue of HpSDH is the lowest, thus the catalytic efficiency of HpSDH is lowerthan those of other SDHs. Notably, the ... system. The enzymatic characterization of HpSDH demonstrates its activity with kcat of 7.7 s)1and Km of 0.148 mmtoward shikimate, kcat of 7.1 s)1and Km of 0.182 mm toward NADP, kcat of 5.2...
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Tài liệu Báo cáo khoa học: Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean) ) coordination structure of the heme complex and catabolism of heme docx
... information on the heme pocketstructure. As shown in Table 2, the hyperfine splittingconstants of the 15N nucleus of the distal NO and of the 14N nucleus of the proximal His of the GmHO-1complex ... characteristics of the heme pocket of GmHO-1 might be associatedwith the smaller values of Kazidefor GmHO-1 andSynHO-1, and with the ferric character of the heme. The Kimidazolevalues of the three ... the heme–rHO-1 reaction, because chelating of the ferric ironenhances the bilirubin yield of the GmHO-1 andSynHO-1 reactions to a similar level as that with the rHO-1 reaction (Table 5). The...
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Tài liệu Báo cáo khoa học: Thermodynamic characterization of interleukin-8 monomer binding to CXCR1 receptor N-terminal domain ppt
... interactions of apolar resi-dues in the complex, and further propose that the ther-modynamic basis of the binding of all chemokineligands to their receptor N-domains is similar to thatobserved for the ... to the receptor CXCR1 N-domain. The binding isotherm of IL-8(1–66) and the trappedL25NMe monomers to the CXCR1 N-domain areshown in Fig. 2. The upper panels show the thermo-grams, and the ... this case, the measured enthalpy of binding is linearly related to the intrinsic ionizationenthalpy of the buffer. The relationship between the ionization enthalpy of the buffer and the measuredenthalpy...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... 4 °C. The supernatant was transferred to an HPLC vial. The products of the reaction were detected at 260 nm using the HPLC system described above for the synthesis of the CoA-esters. The assay ... deficiencies of each of the six dif-ferent steps within the leucine degradation pathway(Fig. 1) cause their own characteristic disease [1]. The enzymes of this pathway are primarily located in the mitochondria. ... 2 mM of the respective isomers of 3-MG-CoA in a total volume of 25 lL. The reaction was started by addition of AUH (1 lg), incubated for 1 h and the CoA products were HPLC-detected by their...
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Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc
... Myco-plasma penetrans and Spiroplasma kunkelii, the genomes of the members of the mollicutes group (total number, 7 out of 10) are devoid of genes for the biosynthesis of IPP andDMAPP. The same is ... of the enzyme for that cofactor and the apparent absence of a redox process as part of the catalyticcycle. The substrate binding site of the type II enzymeremains veiled. However, a patch of ... 2661FMN, we observed the appearance of the signals of bothmethyl groups and of the methine group of the enzymat-ically formed DMAPP (Fig. 4A). Concomitantly, the signals of IPP were progressively...
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Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebrafish (Danio rerio) doc
... symmetry axis. The labe-led specialized large and elongated cells of the anter-ior part of the floor-plate were positioned at the base of the commissure separating the two lobes of the mesencephalic ... between them, in the central portion of the fin, or in the epithelial cell layer surrounding the fin (Fig. 6C, insert). Lateral line neuromasts of the head region coexpressed prp2 and prp3 transcripts ... in the process of oral prion infection, this find-ing might help clarify the entry and routing of PrPScin the early steps of infection. Our observations of a prp2hybridization signal in the...
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