... of Met8P has shown that this protein hasan aspartate residue (Asp141), which is importantfor both chelatase and dehydrogenase function [17];interestingly, this aspartate, Asp129, is also conserved98kDaM ... Authors Journal compilation ª 2010 FEBS 4955 NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis Shilpa Bali1, Martin J. Warren2and Stuart ... encodes a ( 42 kDa) protein that has anN-terminal signal sequence suggestive of a location in the periplasm. On the other hand, in Ps. aeruginosa(PAO1), NirF has no apparent signal sequence andtherefore...