β and smad family proteins in bmmcs
Báo cáo y học: " Differential expression of Toll-like receptors on human alveolar macrophages and autologous peripheral monocytes" pdf
Ngày tải lên :
12/08/2014, 14:20
... cells/mL in three mL culture medium and incubated in six-well plates for one, four and seven days Cells were harvested using cell lifters (Corning Inc., Acton, MA), resuspended in culture medium, and ... specific antibody staining and in order to block nonspecific Fc receptor binding, 10 bronchoalveolar cells and monocyte-derived macrophages were incubated in × phosphate buffered saline (Cambrex, Walkersville, ... from infected and dying cells) and that the ligand is then transferred from the cell surface into the intracellular compartment This may provide an extra safety step prior to inducing the inflammatory...
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Báo cáo y học: "Strong inhibition of TNF-α production and inhibition of IL-8 and COX-2 mRNA expression in monocyte-derived macrophages by RWJ 67657, a p38 mitogen-activated protein kinase (MAPK) inhibitor" ppt
Ngày tải lên :
09/08/2014, 01:23
... cells and in cell lines [9] In the RA synovium, p38 MAPK is predominantly activated in endothelial cells and in the lining layer [10] Inhibition of p38 MAPK therefore could provide an interesting ... p38 α and β, and has no activity for other kinases [13] The first study investigating the pharmacokinetics and pharmacodynamics of RWJ 67657 to be done in humans showed that a single oral intake ... induced by LPS stimulation, and no inhibition by p38 MAPK inhibitor was observed This latter finding is in accordance with the study by Lai and co-workers showing that LPS induction of MMP9 in...
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Báo cáo khoa học: "Time Course Study of Cytokine mRNA Expression in LPS-Stimulated Porcine Alveolar Macrophages" pot
Ngày tải lên :
07/08/2014, 15:20
... 98 In- Soo Choi, Na-Ri Shin, Sung-Jae Shin, Deog-Yong Lee, Young-Wook Cho and Han-Sang Yoo L-glutamine-containing RPMI 1640 (Gibco BRL, Grand Island, NY) supplemented with 10% fetal bovine serum ... against the stimulation times of the kinetics of porcine cytokine mRNA expression in alveolar macrophages In this study, we examined seven porcine cytokine expression levels over a time course in ... each cytokine gene was normalized against that of the cyclophilin A gene The final expression of each cytokine gene was determined by subtracting the expression level of a cytokine in control...
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Báo cáo y học: "DHEA-dependent and organ-specific regulation of TNF-α mRNA expression in a murine polymicrobial sepsis and trauma model" pps
Ngày tải lên :
13/08/2014, 18:22
... pro- and anti-inflammatory cytokines influence the expression levels of each other Thus, high initial TNF-α level may result in an increased production of antiinflammatory cytokines that in turn ... receptor β by DHEA has been determined [34] Plasma levels of TNF-α peak a few hours after a traumatic or septic insult and decline afterwards At the time points determined in this study (48 hours and ... were inverted in liver, showing an increased expression of TNF-α in the CLP-vehicle group DHEA caused a return to levels as observed in the sham groups (Figure 5a) We determined differences in...
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báo cáo khoa học: " Increased IL-10 mRNA expression in tumorassociated macrophage correlated with late stage of lung cancer" pps
Ngày tải lên :
10/08/2014, 10:21
... IL-10, cathepsin B and CD68 in macrophage A-B, High IL-10 expression in macrophage, A, IL-10 staining in macrophage (strong positivity); B, CD68 staining C-D, Cathepsin B expression in macrophage; ... cathepsin B staining in macrophage (most cells were moderate positivity, only a few cells were strong staining); D, CD68 staining Scale bar indicates 50 μm Original magnification, × 400 and stromal(including ... examine the expression of IL-10, cathepsin B in TAMs Samples were fixed in 4% formaldehyde in PBS (pH 7.2) and paraffin embedded 4-μm thickness was cut from each paraffin block After dewaxing and...
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Retrovirology Research BioMed Central Open Access APOBEC3G mRNA expression in exposed ppt
Ngày tải lên :
12/08/2014, 23:20
... hA3G by inducing its ubiquitination and degradation through the Vif protein [5-7] In vivo, the significance of hA3G-induced hypermutation as a protecting agent of clinical pathogenesis and HIV ... and significance marked Horizontal lines show the median, sloped solid line is the linear regression, and the flanking dashed lines show the 95% confidence interval for the mean Horizontal lines ... increased hA3G mRNA levels are in agreement with previous reports that examined CD3 and CD28-stimulated PBMCs in long term non-progressors[8] and in ES individuals[10] Here we found that ES and...
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Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt
Ngày tải lên :
21/02/2014, 15:20
... function and regulation of mitochondrial uncoupling proteins and J Biol Chem 274, 26003–26007 Ó FEBS 2002 19 Echtay, K.S., Winkler, E., Frischmuth, K & Klingenberg, M (2001) Uncoupling proteins and ... The efficacy of infection for varying viral loads was determined by staining for b-Gal (data not shown) Metabolic [35S]methionine labeling and membrane preparation L6 cells were infected with hUCP3, ... three times in NaCl/Pi, then fixed in 4% (w/v) paraformaldehyde/NaCl/Pi for 60 The cells were washed four times in NaCl/Pi and then incubated for 60 in NaCl/Pi containing 0.1 M glycine, pH 8.6...
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Báo cáo khoa học: Ascorbic acid-pretreated quartz enhances cyclo-oxygenase-2 expression in RAW 264.7 murine macrophages pdf
Ngày tải lên :
23/03/2014, 10:20
... production in the RAW cell line and BAL macrophages The transcription factors involved in COX-2 induction and the possible role of specific ROS in triggering the COX-2 synthetic pathway were also investigated ... quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 34 Singh IS, He JR, Calderwood S & Hasday JD (2002) A high affinity HSF-1 binding site in the 5¢-untranslated ... or 18 h (white bars) induced a greater increase in PGE2 production in all samples ranging between a 2.8fold and a 19.6-fold increase compared with the corresponding values in the absence of IFN-c...
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báo cáo hóa học: " Hypoxia-inducible factor-1 (HIF-1) is involved in the regulation of hypoxia-stimulated expression of monocyte chemoattractant protein-1 (MCP-1/CCL2) and MCP-5 (Ccl12) in astrocytes" pdf
Ngày tải lên :
19/06/2014, 22:20
... poly-lysine The cells were cultured in an atmosphere of 5% CO2/95% air at 37°C in D-MEM containing 4.5 g/L glucose, mM glutamine, 25 μg/ml gentamycin (Invitrogen, Burlington, ON), and 10% fetal bovine ... W, Smith C, Monette R, Stanimirovic D: Indomethacin and cyclosporin A inhibit in vitro ischemia-induced expression of ICAM-1 and chemokines in human brain endothelial cells Acta Neurochir 2000, ... HIF-1-binding sites, and the underlined small-letter sequences in the sense and anti-sense primers are KpnI and BglII cutting sites, respectively The PCR fragment was cleaved with KpnI and BglII (Invitrogen)...
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Báo cáo y học: "Expression of CD147 on monocytes/macrophages in rheumatoid arthritis: its potential role in monocyte accumulation and matrix metalloproteinase production" ppt
Ngày tải lên :
09/08/2014, 06:23
... than in OA and AS synovium CD147 was expressed predominantly on the macrophage-like cells and fibroblast-like synovial cells in the lining and sublining layers (Fig 2) MMP-1 was expressed predominantly ... CD147), matrix metalloproteinase-1 (RA MMP-1), tissue inhibitor of metalloproteinases-1 (RA TIMP-1) and blank contrast (RA blank) in the lining and sublining layers in RA synovium (× 400) The ... (green) and CD147+ (red) cells, and the co-expression of CD68+CD147+ cells (yellow) in the lining and sublining layers of rheumatoid arthritis synovium is apparent Immunohistochemistry staining of...
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Báo cáo y học: "Identification of intra-group, inter-individual, and gene-specific variances in mRNA expression profiles in the rheumatoid arthritis synovial membrane" pot
Ngày tải lên :
09/08/2014, 10:23
... IL13 and CXCL13 (cytokine–cytokine receptor interactions) The mean gene expression (blue line) and the intra-group inter-individual variances in RA and NC synovial membrane (red bar) are indicated, ... [2-8] and participate in maintaining joint inflammation, degradation of extracellular matrix (ECM) components, and invasion of cartilage and bone [2,4] as well as fibrosis of the affected joints ... levels of extracellular matrix proteins in response to interleukin J Clin Invest 1992, 90:1479-1485 Firestein GS, Manning AM: Signal transduction and transcription factors in rheumatic disease Arthritis...
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Báo cáo y học: " Early Heat shock protein and heat shock factor 1 expression and localization in vaccinia virus infected human monocyte derived macrophage" ppt
Ngày tải lên :
11/08/2014, 08:21
... Figure virus-induced HSE binding activity in macrophages and a human hepatoma cell line Vaccinia3 Vaccinia virus-induced HSE binding activity in macrophages and a human hepatoma cell line (A) Macrophages ... interleukin 12 and interleukin 10 expression in vaccinia virus-infected human monocytes and U-937 cell line Cytokine 2000, 12:900-908 Jindal S, Young RA: Vaccinia virus infection induces a stress ... heat shock proteins in many cell lines [9] The heat shock proteins can be induced in vitro following infection by a variety of viruses [10] such as Ad5 and HSV-1 which have been shown to induce synthesis...
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Báo cáo y học: "Collagen V-induced nasal tolerance downregulates pulmonary collagen mRNA gene and TGF-beta expression in experimental systemic sclerosis" doc
Ngày tải lên :
12/08/2014, 14:20
... collagen chain Complete primary structure, expression domains in adult and developing tissues, and comparison to the structures and expression domains of the other types V and XI procollagen chains J ... collagenous fibers in peribronchovascular and septal pulmonary interstitium, Massons trichrome was used to stain the collagen-containing fibers in blue Also, the Picrosirius staining method [19] ... works [21] To quantify stained cells, a point-counting stereologic method [19] was employed using a reticulum formed by 100 points and 50 lines, each measuring 25 m in length, adapted to a conventional...
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Báo cáo y học: "Differential gene expression in HIV/SIV-associated and spontaneous lymphomas"
Ngày tải lên :
02/11/2012, 11:08
... DLBCLs (# 3, 7, 8, 10), and in some FL (# 4) and HD (# 14) The SMG-1 gene was highly transcribed in DLBCL # 7, in Int J Med Sci 2005 FLs # 5, and in HDs # 13, 14 In lymphomas # and SMG-1 transcripts ... of immunoglobulin gene, γIg – the γ-chain of immunoglobulin gene, IFN-ind – the interferone-inducible gene 6-16, IL4R – the interleukin receptor gene, TAP2 – the transport protein gene, hnRNP ... protein SET/TAF- 1β is a member of the INHAT (inhibitor of histone acetyltransferase) complex SET is also an inhibitor of protein phosphatase 2A [32] Inhibition of this phosphatase induces an increase...
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Influence of Polyferric Sulfate Coagulant on the amoA mRNA Expression of Ammonia Oxidizer in Activated Sludge
Ngày tải lên :
05/09/2013, 10:15
... experiment are shown in Fig Gradual decrease in NH4+-N and increase in NO2+3 N were observed just after the beginning of the experiment in control and both runs However, decrease in nitrification ... difference in the phosphorus removal efficiency in Runs and Effect of polyferric sulfate on DOC removal was also examined Almost all DOC was removed in 200 min, and there was no difference in DOC ... from 100 after starting the experiment Although complete nitrification occurred in control and Run at 600 min, nitrification rate was slow in Run and 40% of ammonia remained in Run Therefore, nitrification...
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Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
Ngày tải lên :
12/02/2014, 10:20
... following IMPACT-CN system manufactures instruction by using chitin beads column chromatography The obtained purified recombinant MCoTI-II showing inhibitory activity against trypsin ,and proteinases ... the 58 kD protein band was found in induced sample as the major band, acounted for 80% of total protein (lane 1,3,5 and 6) 58 kD Fig 10 SDS PAGE pattern of PI-17 proteins Mc Standard molecular ... Standard molecular markers 2, Uninduced 1, 3, 5, Induced (containing 58kD fusion protein band) Cells were broken by sonication in 10mM Tris-HCl buffer pH8 containing 1mM EDTA (TE buffer) After...
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Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt
Ngày tải lên :
18/02/2014, 18:20
... molecules and adhesion molecules, and to serve as antigen-presenting cells [18–20] These chemokines and in ammatory proteins may recruit macrophages from the blood vessels, leading to local in ammation ... SCs have been implicated in human in ammatory demyelinating neuropathies such as ´ Guillain–Barre syndrome and chronic in ammatory demyelinating polyneuropathy [31] In experimental autoimmune ... of SAPK ⁄ JNK in mediating LPS-induced ICAM-1 expression in SCs JNK phosphorylates c-Jun and ATF-2 and increases their ability to activate transcription, leading to c-jun induction and subsequent...
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Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx
Ngày tải lên :
19/02/2014, 02:20
... Ne-carboxymethyl-lysines and Ne-carboxyethyl-lysines and pentosidine) being known to accumulate with age on tissue proteins, and at an increased rate in LDL and atherosclerotic lesions in people with ... MF, Klein RL & Virella G (1996) Modification of lipoproteins in diabetes Diabetes Metab Rev 12, 69–90 10 Tomkin GH & Owens D (2001) Abnormalities in apo B-containing lipoproteins in diabetes and ... (1994) Binding and modification of proteins by methylglyoxal under physiological conditions A kinetic and mechanistic study with Nalpha-acetylarginine, Nalpha-acetylcysteine, and Nalpha-acetyllysine,...
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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
Ngày tải lên :
19/02/2014, 16:20
... barbed-end actin and determine the level and distribution of F-actin [34] Therefore, it is tempting to speculate that interactions between monomeric G-actin and actin-binding proteins are a potential ... data are in line with previous observations indicating that physiological activators of the p38 and PtdIns 3-kinase pathways including thrombin, dexamethasone, angiotensin II and prostaglandins stimulate ... for 30 and then fixed, permeabilized and stained for F-actin with rhodamine-conjugated phalloidin The effects of toxin B and latrunculin B on the actin filaments was examined by preincubating the...
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Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf
Ngày tải lên :
19/02/2014, 16:20
... role in the regulation of cell proliferation, including SMC, by inhibiting signalling molecules [45–47] Caveolin-3 is the main isoform in muscle cells, including SMC [30] Caveolin-1 and caveolin-3 ... n-6 and n-3 PUFAs on IL-1b-induced Egr-1 mRNA Caveolins are scaffolding proteins that interact and negatively regulate many components through the p42/44 MAP kinase pathway [28] Interestingly, ... of cyclin D1 it until it was barely detectable As the retinoblastoma protein, Rb, is a key target of cyclin D1/cyclin-dependent kinase complexes, we investigated whether inhibiting cyclin D1 with...
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