use of differentiating embryonic stem cells in the parkinsonian mouse model pdf

báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc

Ngày tải lên : 18/06/2014, 15:20

... expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly targeted by a specific miRNA is a major obstacle in understanding the miRNA ... names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture ... detected in adult cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in two types of adult cells HMVEC and HUVEC Differences in the...

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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx

Ngày tải lên : 07/03/2014, 12:20

... analyzed the interaction of RNA Pol II and TATA binding protein (TBP) as well as the appearance of modiﬁed histones within the globin locus during the course of differentiation using the ChIP ... analyzed the interaction of RNA Pol II with the b-globin gene locus in mouse embryonic ﬁbroblasts (MEFs) and OP9 stromal cells (OP9) These cells were used in our subsequent studies to support the ... chromatin-opening activity In other words, HS3 could maintain a small accessible region in the globin locus during the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3...

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Báo cáo y học: "Therapeutic potential of human umbilical cord mesenchymal stem cells in the treatment of rheumatoid arthritis" pps

Ngày tải lên : 12/08/2014, 15:22

... T cells in vivo [38] The upregulation of IL-10 is in line with the induction of Tregs in vitro and in vivo in our study Moreover, in our experiments we did not find UC-MSCs in the joint of the ... damage [31] The MMPs are key mediators of the invasive phenotype of FLSs [32] Therefore, we further investigated the effect of UC-MSCs on the invasive behavior of FLSs by the Cell Migration/Invasion ... UC-MSCs maintained such inhibitory effects, suggesting that the transplantation of these cells is practicable and effective for treatment of RA Interestingly, the invasive behavior of FLSs was inhibited...

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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx

Ngày tải lên : 13/08/2014, 23:20

... were obtained for further analysis Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic stem cells without production of whole ... The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics ... which are totipotent In contrast to the pluripotent cells obtained from the blastocyst ICM, one or two of these blastomeres may be biopsied without compromising the integrity of the sampled embryo...

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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity

Ngày tải lên : 11/09/2015, 09:16

... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving the ... expansion Cytotherapy 75 Du F, Zhou Z, Mo ZY, et al 2006;Mixed macromolecular crowding accelerates the refolding of rabbit muscle creatine kinase: implications for protein folding in physiological...

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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders

Ngày tải lên : 16/09/2015, 15:55

... categories of cells make up the human body viz., germ cells, somatic cells and stem cells Somatic cells include the bulk of the cells that make up the human adult and each of these cells in their ... practice involves either the injection of stem cells directly into the targeted tissue, or injection of the stem cells into the bloodstream without any guarantee that they will actually home in the ... organism thereby ensuring the continuation of the germ line In fact the zygote is at the top of the hierarchical stem cell tree being the most primitive and producing the first two cells by cleavage...

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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS

Ngày tải lên : 09/10/2015, 11:24

... understanding of the mechanism underlying the lineage specific differentiation of hES cells is mandatory 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are ... be eliminated very soon Other challenges in the use of ESC include controlling lineage specific differentiation and elimination of residual stem cells Thus to obtain a purified population of osteoblasts ... Culture and maintenance of Human Embryonic Stem Cells Human embryonic stem cells (hESCs) used in this study were the H1 cell lines (isolated and established at the University of Wisconsin) with normal...

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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"

Ngày tải lên : 31/10/2012, 16:57

... culture within bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton ... layer of mitomycin C-inactivated murine embryonic fibroblast feeder (MEF) cells [7, 8] These were harvested from CF1 inbred mouse strain purchased from Charles River Laboratories Inc (Wilmington, ... Virtually all of the detached cells were determined to be non-viable with tryphan blue staining (data not shown) All of the detached cells were washed off prior to the MTT assay From the raw absorbance...

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báo cáo hóa học:" Serous papillary adenocarcinoma possibly related to the presence of primitive oocyte-like cells in the adult ovarian surface epithelium: a case report" docx

Ngày tải lên : 20/06/2014, 08:20

... VASA-positive cells (arrows) in the ovarian surface epithelium and cortex (A, B) Smaller cells with diameters of to μm in the epithelium (C-F) Bigger cells with diameters of 10 μm in the epithelium ... search for the presence of putative stem cells The analysis confirmed the presence of round, SSEA-4 and SOX-2-positive primitive oocyte-like cells in the ovarian surface epithelium These cells resembled ... deeper into the ovarian cortex The nuclei of hypertrophic/necrotic cells consisted of degraded chromatin as revealed by DAPI staining (Figure 6C) This type of cells is usually not present in the...

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Báo cáo khoa học: " Changes of gastrointestinal argyrophil endocrine cells in the osteoporotic SD rats induced by ovariectomy" ppt

Ngày tải lên : 07/08/2014, 17:23

... their density would reflect the change in the capacity of producing these hormones [8] In the present study, the changes of the argyrophil endocrine cells in the GI tract of SD rat after ovariectomy ... type cells which were situated in the intestinal regions In addition, the endocrine cells are regarded as the anatomical units responsible for the production of gut hormones, and a change in their ... technique, the Grimelius method The general distribution of the argyrophil cells in the GI tract 186 Sae-Kwang Ku et al Fig Argyrophil endocrine cells in the GI tract of OVX; Most of argyrophil cells...

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Báo cáo khoa học: "Changes of gastrointestinal argyrophil endocrine cells in the COLO205 tumor-implanted Balb/c-nu/nu mice" pps

Ngày tải lên : 07/08/2014, 18:21

... lihporygra ,yduts siht nI stluseR 962 giF ecnereffid tnacifingis a deredisnoc saw 50.0 naht ssel fo eulav-p a dna )ASU ,SSPS ;3.1.6 esaeleR( swodniW rof SSPS htiw atad fo ecnacifingis eht ezylana ot ... etanobracylop rep detacolla erew slaminA syad rof noitazitamilcca retfa desu erew )napaJ ,reviR selrahC ;tpiecer nopu dlo kw-6( ecim elamef un/un-c/blaB FPS neT o slamina latnemirepxE sdohteM dna slairetaM ... eht nI mueli dna munujej eht rof tpecxe tcart lanitsetni eritne eht ni desaerced )50.0 < ro 10.0 < ( yltnacifingis erew sllec lihporygra ,noitatnalpmi -502OLOC fo stluser sa ,revewoH mahS fo taht...

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Báo cáo y học: "Clinical use of a modified release methylphenidate in the treatment of childhood attention deficit hyperactivity disorder" pps

Ngày tải lên : 09/08/2014, 01:21

... licensed in the UK for the management of ADHD in school-age children and young people, and are effective in controlling ADHD symptoms [9] The guidelines produced by the UK National Institute of Clinical ... by the ADHD nurse specialist, using a structured form The process includes observation of the child in the playground to observe social interaction with peers, observation of the child during ... shown the relative efficacies over different periods of the day, depending on the release profile [21-23] Having different MPH-MR proportions with differing release profiles allows tailoring of...

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$Báo cáo y học: " Use of intra-medullary stacked nailing in the reduction of proximal plastic deformity in a pediatric Monteggia fracture: a case report" pptx$

Báo cáo y học: " Use of intra-medullary stacked nailing in the reduction of proximal plastic deformity in a pediatric Monteggia fracture: a case report" pptx

Ngày tải lên : 11/08/2014, 00:23

... resulting in a tight intra-medullary fit that reduced Page of the ulna and the radio-capitellar joint concomitantly (Figure 4) A plaster of Paris cast was applied to maintain the right forearm in ... K-wires into the proximal fragment These were used as a joystick to reduce the plastic bow of the ulna and were then advanced down the medulla (Figure 3) A third K-wire was inserted in a similar ... inserted longitudinally into the proximal fragment were used as a joystick to partially reduce the ulnar bowing, which was further reduced by passing the K-wires distally A third K-wire was then...

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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx

Ngày tải lên : 11/08/2014, 12:21

... CTCF Figure The combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation ... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein ... through the incomplete erasure of marks characteristic of the somatic cell type of origin (somatic memory) during iPSC reprogramming, or defects in the re-establishment of hESC-like patterns in iPSCs,...

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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt

Ngày tải lên : 11/08/2014, 20:21

... cultivation in the presence of osteoinductive stimuli Numerous living cells were detected in the mineralized centre of the spheres by means of toluidine blue staining (Figure 1) Generally, the mineralization ... totipotent embryonic stem cells cultured in three-dimensional micromass technique in the absence of any scaffold The osteogenic differentiation of the cells was induced by the addition of dexamethasone, ... toluidine blue followed by counterstaining with alizarin red Shown is evidence for the mineralization in the centre of the micromasses, which were stained in red alizarin red (Figure 1) The SEM...

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Báo cáo y học: " In situ detection of Gag-specific CD8+ cells in the GI tract of SIV infected Rhesus macaques" pptx

Ngày tải lên : 12/08/2014, 23:23

... localization of virus specific CD8+ T cells may prove useful in the study of the pathogenesis of SIV infection and the role vaccines and immunotherapy may play in altering the CD8+ T cell response in the ... T cells in these locations to have the potential to control the infection at its initial site In two of the three RMs, a similar percentage of Gag CM9 positive cells was found in PBMCs as in ... section of the ileum were counted using the particle counting algorithm of public domain imaging software ImageJ Total cells/ mm , CD8 + cells/ mm , Gag CM9 positive cells/ mm and the percentage of Gag...

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Báo cáo y học: " The use of chiropractors by older adults in the United States" potx

Ngày tải lên : 13/08/2014, 14:20

... Among the Oldest Old (AHEAD), which is sponsored by the National Institute on Aging, of the National Institutes of Health (NIH), in the United States The design and sampling approach in the AHEAD ... two final categories of covariates were the use of health services, and the supply of chiropractors in the community There were two measures of self-reported health services use – the number of ... prior studies of chiropractic use in the United States not provide multivariable modeling of factors associated with the use of chiropractors, or the volume of chiropractic visits Finally, although...

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Báo cáo y học: "Stem cells in the genomic age" docx

Ngày tải lên : 14/08/2014, 16:21

... comment stem cells, following the changes in their transcriptional profile as they escape from the stem- cell state and commit to differentiation When two cells chose different fates they must of course ... Reprogramming for pluripotency Austin Smith (Institute for Stem Cell Research, Edinburgh, UK) has developed similar genetic reporter lines for investigating the question of how ES cells commit to the ... fusion system, Smith has found an unexpected role for the homeodomain-containing protein Nanog in this process By boosting levels of Nanog it is possible to increase the efficiency of nuclear...

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Baculovirus mediated genetic modification of human embryonic stem cells

Ngày tải lên : 11/09/2015, 14:38

... expression in human embryonic stem cells for the purpose of genetic modification, thus facilitating the realization of the great potential of these cells in basic researches and clinical applications ... Nevertheless, the promoting effect of including the WPRE or the CMV immediate early gene enhancer in the EF1α promoter-containing expression cassettes was observed in HES-3, especially the combined ... the combined use of the two elements significantly increased the percentage of eGFP-positive cells, even at an MOI of 100, over that seen using the cassettes containing either of the two elements...

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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages

Ngày tải lên : 04/10/2015, 16:03

... development in the mouse In the mouse, haematopoietic lineages first appear in the form of blood islands in the yolk sac, a derivative of the extraembryonic mesoderm by embryonic day E7.5 of gestation ... accomplished in the mouse in 1981 (Evans and Kaufman 1981; Martin 1981) The ease with which mouse embryonic stem cells (mESCs) can be derived and manipulated has made them an ideal model system for the ... Available information on knock-out phenotypes in the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells and their in vitro characterisation Figure 1.2 Lineage tree of...

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